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Q99952

- PTN18_HUMAN

UniProt

Q99952 - PTN18_HUMAN

Protein

Tyrosine-protein phosphatase non-receptor type 18

Gene

PTPN18

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
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    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 2 (25 Nov 2008)
      Previous versions | rss
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    Functioni

    Differentially dephosphorylate autophosphorylated tyrosine kinases which are known to be overexpressed in tumor tissues.

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei197 – 1971SubstrateBy similarity
    Active sitei229 – 2291Phosphocysteine intermediatePROSITE-ProRule annotation
    Binding sitei276 – 2761SubstrateBy similarity

    GO - Molecular functioni

    1. non-membrane spanning protein tyrosine phosphatase activity Source: ProtInc
    2. protein binding Source: IntAct

    GO - Biological processi

    1. peptidyl-tyrosine dephosphorylation Source: GOC
    2. protein dephosphorylation Source: ProtInc

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tyrosine-protein phosphatase non-receptor type 18 (EC:3.1.3.48)
    Alternative name(s):
    Brain-derived phosphatase
    Gene namesi
    Name:PTPN18
    Synonyms:BDP1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 2

    Organism-specific databases

    HGNCiHGNC:9649. PTPN18.

    Subcellular locationi

    Nucleus By similarity. Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA33991.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 460460Tyrosine-protein phosphatase non-receptor type 18PRO_0000094773Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei389 – 3891PhosphotyrosineBy similarity
    Modified residuei426 – 4261PhosphotyrosineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ99952.
    PaxDbiQ99952.
    PRIDEiQ99952.

    PTM databases

    PhosphoSiteiQ99952.

    Expressioni

    Tissue specificityi

    Expressed in brain, colon and several tumor-derived cell lines.1 Publication

    Gene expression databases

    ArrayExpressiQ99952.
    BgeeiQ99952.
    CleanExiHS_BDP1.
    HS_PTPN18.
    GenevestigatoriQ99952.

    Organism-specific databases

    HPAiCAB012174.
    HPA053367.

    Interactioni

    Subunit structurei

    Interacts with PSTPIP1.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PSTPIP1O435864EBI-1384210,EBI-1050964

    Protein-protein interaction databases

    BioGridi117693. 2 interactions.
    IntActiQ99952. 4 interactions.
    MINTiMINT-2815851.
    STRINGi9606.ENSP00000175756.

    Structurei

    Secondary structure

    1
    460
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi11 – 144
    Helixi20 – 223
    Helixi25 – 4319
    Helixi49 – 524
    Turni54 – 563
    Helixi57 – 593
    Helixi69 – 713
    Beta strandi72 – 743
    Helixi79 – 813
    Beta strandi85 – 939
    Beta strandi97 – 1048
    Helixi109 – 1113
    Helixi112 – 12110
    Beta strandi126 – 1294
    Beta strandi133 – 1353
    Beta strandi152 – 1543
    Beta strandi157 – 16812
    Beta strandi171 – 18010
    Beta strandi183 – 19210
    Beta strandi197 – 1993
    Helixi205 – 21814
    Beta strandi225 – 2284
    Beta strandi230 – 2334
    Helixi234 – 25017
    Helixi260 – 2689
    Helixi278 – 29215

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2OC3X-ray1.50A6-299[»]
    4GFUX-ray2.00A6-300[»]
    4GFVX-ray2.10A/B6-300[»]
    ProteinModelPortaliQ99952.
    SMRiQ99952. Positions 7-295.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ99952.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini26 – 291266Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni229 – 2357Substrate bindingBy similarity

    Sequence similaritiesi

    Contains 1 tyrosine-protein phosphatase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5599.
    HOGENOMiHOG000115776.
    HOVERGENiHBG053419.
    InParanoidiQ99952.
    KOiK18024.
    OMAiNCAPLYD.
    OrthoDBiEOG744T8Z.
    PhylomeDBiQ99952.
    TreeFamiTF351977.

    Family and domain databases

    Gene3Di3.90.190.10. 1 hit.
    InterProiIPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    IPR000242. Tyr_Pase_rcpt/non-rcpt.
    [Graphical view]
    PfamiPF00102. Y_phosphatase. 1 hit.
    [Graphical view]
    PRINTSiPR00700. PRTYPHPHTASE.
    SMARTiSM00194. PTPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52799. SSF52799. 1 hit.
    PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q99952-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSRSLDSARS FLERLEARGG REGAVLAGEF SDIQACSAAW KADGVCSTVA    50
    GSRPENVRKN RYKDVLPYDQ TRVILSLLQE EGHSDYINGN FIRGVDGSLA 100
    YIATQGPLPH TLLDFWRLVW EFGVKVILMA CREIENGRKR CERYWAQEQE 150
    PLQTGLFCIT LIKEKWLNED IMLRTLKVTF QKESRSVYQL QYMSWPDRGV 200
    PSSPDHMLAM VEEARRLQGS GPEPLCVHCS AGCGRTGVLC TVDYVRQLLL 250
    TQMIPPDFSL FDVVLKMRKQ RPAAVQTEEQ YRFLYHTVAQ MFCSTLQNAS 300
    PHYQNIKENC APLYDDALFL RTPQALLAIP RPPGGVLRSI SVPGSPGHAM 350
    ADTYAVVQKR GAPAGAGSGT QTGTGTGTGA RSAEEAPLYS KVTPRAQRPG 400
    AHAEDARGTL PGRVPADQSP AGSGAYEDVA GGAQTGGLGF NLRIGRPKGP 450
    RDPPAEWTRV 460
    Length:460
    Mass (Da):50,482
    Last modified:November 25, 2008 - v2
    Checksum:i67ED24A0504D1883
    GO
    Isoform 2 (identifier: Q99952-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         32-138: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:353
    Mass (Da):38,476
    Checksum:i12B3D38CA2A8A7DE
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti356 – 3572VV → EE in CAA56105. (PubMed:8950995)Curated
    Sequence conflicti378 – 3792Missing in CAA56105. (PubMed:8950995)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti193 – 1931M → V.
    Corresponds to variant rs3739124 [ dbSNP | Ensembl ].
    VAR_047651

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei32 – 138107Missing in isoform 2. 1 PublicationVSP_043073Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X79568 mRNA. Translation: CAA56105.1.
    AK303804 mRNA. Translation: BAG64758.1.
    AC132479 Genomic DNA. Translation: AAY24077.1.
    CH471263 Genomic DNA. Translation: EAW55618.1.
    CH471263 Genomic DNA. Translation: EAW55619.1.
    CCDSiCCDS2161.1. [Q99952-1]
    CCDS46410.1. [Q99952-2]
    RefSeqiNP_001135842.1. NM_001142370.1. [Q99952-2]
    NP_055184.2. NM_014369.3. [Q99952-1]
    UniGeneiHs.516390.
    Hs.744861.

    Genome annotation databases

    EnsembliENST00000175756; ENSP00000175756; ENSG00000072135. [Q99952-1]
    ENST00000347849; ENSP00000310092; ENSG00000072135. [Q99952-2]
    GeneIDi26469.
    KEGGihsa:26469.
    UCSCiuc002trb.3. human. [Q99952-2]
    uc002trc.3. human. [Q99952-1]

    Polymorphism databases

    DMDMi215273871.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X79568 mRNA. Translation: CAA56105.1 .
    AK303804 mRNA. Translation: BAG64758.1 .
    AC132479 Genomic DNA. Translation: AAY24077.1 .
    CH471263 Genomic DNA. Translation: EAW55618.1 .
    CH471263 Genomic DNA. Translation: EAW55619.1 .
    CCDSi CCDS2161.1. [Q99952-1 ]
    CCDS46410.1. [Q99952-2 ]
    RefSeqi NP_001135842.1. NM_001142370.1. [Q99952-2 ]
    NP_055184.2. NM_014369.3. [Q99952-1 ]
    UniGenei Hs.516390.
    Hs.744861.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2OC3 X-ray 1.50 A 6-299 [» ]
    4GFU X-ray 2.00 A 6-300 [» ]
    4GFV X-ray 2.10 A/B 6-300 [» ]
    ProteinModelPortali Q99952.
    SMRi Q99952. Positions 7-295.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 117693. 2 interactions.
    IntActi Q99952. 4 interactions.
    MINTi MINT-2815851.
    STRINGi 9606.ENSP00000175756.

    PTM databases

    PhosphoSitei Q99952.

    Polymorphism databases

    DMDMi 215273871.

    Proteomic databases

    MaxQBi Q99952.
    PaxDbi Q99952.
    PRIDEi Q99952.

    Protocols and materials databases

    DNASUi 26469.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000175756 ; ENSP00000175756 ; ENSG00000072135 . [Q99952-1 ]
    ENST00000347849 ; ENSP00000310092 ; ENSG00000072135 . [Q99952-2 ]
    GeneIDi 26469.
    KEGGi hsa:26469.
    UCSCi uc002trb.3. human. [Q99952-2 ]
    uc002trc.3. human. [Q99952-1 ]

    Organism-specific databases

    CTDi 26469.
    GeneCardsi GC02P131113.
    HGNCi HGNC:9649. PTPN18.
    HPAi CAB012174.
    HPA053367.
    MIMi 606587. gene.
    neXtProti NX_Q99952.
    PharmGKBi PA33991.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5599.
    HOGENOMi HOG000115776.
    HOVERGENi HBG053419.
    InParanoidi Q99952.
    KOi K18024.
    OMAi NCAPLYD.
    OrthoDBi EOG744T8Z.
    PhylomeDBi Q99952.
    TreeFami TF351977.

    Miscellaneous databases

    ChiTaRSi PTPN18. human.
    EvolutionaryTracei Q99952.
    GeneWikii PTPN18.
    GenomeRNAii 26469.
    NextBioi 48711.
    PROi Q99952.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q99952.
    Bgeei Q99952.
    CleanExi HS_BDP1.
    HS_PTPN18.
    Genevestigatori Q99952.

    Family and domain databases

    Gene3Di 3.90.190.10. 1 hit.
    InterProi IPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    IPR000242. Tyr_Pase_rcpt/non-rcpt.
    [Graphical view ]
    Pfami PF00102. Y_phosphatase. 1 hit.
    [Graphical view ]
    PRINTSi PR00700. PRTYPHPHTASE.
    SMARTi SM00194. PTPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52799. SSF52799. 1 hit.
    PROSITEi PS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of the PEST family protein tyrosine phosphatase BDP1."
      Kim Y.W., Wang H.-Y., Sures I., Lammers R., Martell K.J., Ullrich A.
      Oncogene 13:2275-2279(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, CHARACTERIZATION.
      Tissue: Brain.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Liver.
    3. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
      Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
      , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
      Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.
    6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "Large-scale structural analysis of the classical human protein tyrosine phosphatome."
      Barr A.J., Ugochukwu E., Lee W.H., King O.N.F., Filippakopoulos P., Alfano I., Savitsky P., Burgess-Brown N.A., Mueller S., Knapp S.
      Cell 136:352-363(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 6-299.

    Entry informationi

    Entry nameiPTN18_HUMAN
    AccessioniPrimary (citable) accession number: Q99952
    Secondary accession number(s): B4E1E6, Q53P42
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 27, 2002
    Last sequence update: November 25, 2008
    Last modified: October 1, 2014
    This is version 127 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 2
      Human chromosome 2: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3