Q99952 (PTN18_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine-protein phosphatase non-receptor type 18
EC=3.1.3.48

Alternative name(s):
Brain-derived phosphatase

Gene names

Name:	PTPN18
Synonyms:	BDP1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	460 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Differentially dephosphorylate autophosphorylated tyrosine kinases which are known to be overexpressed in tumor tissues.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Subunit structure	Interacts with PSTPIP1 By similarity.
Subcellular location	Nucleus By similarity. Cytoplasm By similarity.
Tissue specificity	Expressed in brain, colon and several tumor-derived cell lines. Ref.1
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Non-receptor class 4 subfamily. Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Molecular function	Hydrolase Protein phosphatase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	non-membrane spanning protein tyrosine phosphatase activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q99952-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q99952-2)

The sequence of this isoform differs from the canonical sequence as follows:
     32-138: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 460

460

Tyrosine-protein phosphatase non-receptor type 18

PRO_0000094773

Regions

Domain

26 – 291

266

Tyrosine-protein phosphatase

Region

229 – 235

7

Substrate binding By similarity

Sites

Active site

229

1

Phosphocysteine intermediate By similarity

Binding site

197

1

Substrate By similarity

Binding site

276

1

Substrate By similarity

Amino acid modifications

Modified residue

389

1

Phosphotyrosine By similarity

Modified residue

426

1

Phosphotyrosine By similarity

Natural variations

Alternative sequence

32 – 138

107

Missing in isoform 2.

VSP_043073

Natural variant

193

1

M → V.
Corresponds to variant rs3739124 [ dbSNP | Ensembl ].

VAR_047651

Experimental info

Sequence conflict

356 – 357

2

VV → EE in CAA56105. Ref.1

Sequence conflict

378 – 379

2

Missing in CAA56105. Ref.1

Secondary structure

1

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460

Helix Strand Turn

Details...

Helix

11 – 14

4

Helix

25 – 43

19

Helix

49 – 52

4

Turn

54 – 56

3

Helix

57 – 59

3

Helix

69 – 71

3

Beta strand

72 – 74

3

Helix

79 – 81

3

Beta strand

85 – 93

9

Beta strand

97 – 104

8

Helix

109 – 111

3

Helix

112 – 121

10

Beta strand

126 – 129

4

Beta strand

133 – 135

3

Beta strand

152 – 154

3

Beta strand

157 – 168

12

Beta strand

171 – 180

10

Beta strand

183 – 192

10

Beta strand

197 – 199

3

Helix

205 – 218

14

Beta strand

225 – 228

4

Beta strand

230 – 233

4

Helix

234 – 250

17

Helix

260 – 268

9

Helix

278 – 292

15

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 25, 2008. Version 2. Checksum: 67ED24A0504D1883	FASTA	460	50,482
	10 20 30 40 50 60 MSRSLDSARS FLERLEARGG REGAVLAGEF SDIQACSAAW KADGVCSTVA GSRPENVRKN 70 80 90 100 110 120 RYKDVLPYDQ TRVILSLLQE EGHSDYINGN FIRGVDGSLA YIATQGPLPH TLLDFWRLVW 130 140 150 160 170 180 EFGVKVILMA CREIENGRKR CERYWAQEQE PLQTGLFCIT LIKEKWLNED IMLRTLKVTF 190 200 210 220 230 240 QKESRSVYQL QYMSWPDRGV PSSPDHMLAM VEEARRLQGS GPEPLCVHCS AGCGRTGVLC 250 260 270 280 290 300 TVDYVRQLLL TQMIPPDFSL FDVVLKMRKQ RPAAVQTEEQ YRFLYHTVAQ MFCSTLQNAS 310 320 330 340 350 360 PHYQNIKENC APLYDDALFL RTPQALLAIP RPPGGVLRSI SVPGSPGHAM ADTYAVVQKR 370 380 390 400 410 420 GAPAGAGSGT QTGTGTGTGA RSAEEAPLYS KVTPRAQRPG AHAEDARGTL PGRVPADQSP 430 440 450 460 AGSGAYEDVA GGAQTGGLGF NLRIGRPKGP RDPPAEWTRV
	Isoform 2 [UniParc]. Checksum: 12B3D38CA2A8A7DE	FASTA	353	38,476
	10 20 30 40 50 60 MSRSLDSARS FLERLEARGG REGAVLAGEF SKRCERYWAQ EQEPLQTGLF CITLIKEKWL 70 80 90 100 110 120 NEDIMLRTLK VTFQKESRSV YQLQYMSWPD RGVPSSPDHM LAMVEEARRL QGSGPEPLCV 130 140 150 160 170 180 HCSAGCGRTG VLCTVDYVRQ LLLTQMIPPD FSLFDVVLKM RKQRPAAVQT EEQYRFLYHT 190 200 210 220 230 240 VAQMFCSTLQ NASPHYQNIK ENCAPLYDDA LFLRTPQALL AIPRPPGGVL RSISVPGSPG 250 260 270 280 290 300 HAMADTYAVV QKRGAPAGAG SGTQTGTGTG TGARSAEEAP LYSKVTPRAQ RPGAHAEDAR 310 320 330 340 350 GTLPGRVPAD QSPAGSGAYE DVAGGAQTGG LGFNLRIGRP KGPRDPPAEW TRV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the PEST family protein tyrosine phosphatase BDP1." Kim Y.W., Wang H.-Y., Sures I., Lammers R., Martell K.J., Ullrich A. Oncogene 13:2275-2279(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, CHARACTERIZATION. Tissue: Brain.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Liver.
[3]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Leukemic T-cell.
[6]	"Large-scale structural analysis of the classical human protein tyrosine phosphatome." Barr A.J., Ugochukwu E., Lee W.H., King O.N.F., Filippakopoulos P., Alfano I., Savitsky P., Burgess-Brown N.A., Mueller S., Knapp S. Cell 136:352-363(2009) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 6-299.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X79568 mRNA. Translation: CAA56105.1.
AK303804 mRNA. Translation: BAG64758.1.
AC132479 Genomic DNA. Translation: AAY24077.1.
CH471263 Genomic DNA. Translation: EAW55618.1.
CH471263 Genomic DNA. Translation: EAW55619.1.

IPI

IPI00152019.
IPI00219132.

RefSeq

NP_001135842.1. NM_001142370.1.
NP_055184.2. NM_014369.3.

UniGene

Hs.516390.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2OC3	X-ray	1.50	A	6-299	[»]

ProteinModelPortal

Q99952.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q99952. 1 interaction.

STRING

9606.ENSP00000175756.

PTM databases

PhosphoSite

Q99952.

Polymorphism databases

DMDM

215273871.

Proteomic databases

PaxDb

Q99952.

PRIDE

Q99952.

Protocols and materials databases

DNASU

26469.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000175756; ENSP00000175756; ENSG00000072135.
ENST00000347849; ENSP00000310092; ENSG00000072135.

GeneID

26469.

KEGG

hsa:26469.

UCSC

uc002trc.3. human.

Organism-specific databases

CTD

26469.

GeneCards

GC02P131113.

HGNC

HGNC:9649. PTPN18.

HPA

CAB012174.

MIM

606587. gene.

neXtProt

NX_Q99952.

PharmGKB

PA33991.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG5599.

HOGENOM

HOG000115776.

HOVERGEN

HBG053419.

InParanoid

Q99952.

KO

K01104.

OMA

GLGFNLR.

OrthoDB

EOG4X0MSD.

PhylomeDB

Q99952.

Gene expression databases

ArrayExpress

Q99952.

Bgee

Q99952.

CleanEx

HS_BDP1.
HS_PTPN18.

Genevestigator

Q99952.

GermOnline

ENSG00000072135. Homo sapiens.

Family and domain databases

InterPro

IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
[Graphical view]

Pfam

PF00102. Y_phosphatase. 1 hit.
[Graphical view]

PRINTS

PR00700. PRTYPHPHTASE.

SMART

SM00194. PTPc. 1 hit.
[Graphical view]

PROSITE

PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

PTPN18. human.

EvolutionaryTrace

Q99952.

GenomeRNAi

26469.

NextBio

48711.

SOURCE

Search...

Entry information

Entry name

PTN18_HUMAN

Accession

Primary (citable) accession number: Q99952
Secondary accession number(s): B4E1E6, Q53P42

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	November 25, 2008
Last modified:	May 1, 2013
This is version 114 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.