Q99952 (PTN18_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine-protein phosphatase non-receptor type 18
EC=3.1.3.48

Alternative name(s):
Brain-derived phosphatase

Gene names

Name:	PTPN18
Synonyms:	BDP1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	460 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Differentially dephosphorylate autophosphorylated tyrosine kinases which are known to be overexpressed in tumor tissues.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Subunit structure	Interacts with PSTPIP1 By similarity.
Subcellular location	Nucleus By similarity. Cytoplasm By similarity.
Tissue specificity	Expressed in brain, colon and several tumor-derived cell lines. Ref.1
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Non-receptor class 4 subfamily. Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Polymorphism
Molecular function	Hydrolase Protein phosphatase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	non-membrane spanning protein tyrosine phosphatase activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 460

460

Tyrosine-protein phosphatase non-receptor type 18

PRO_0000094773

Regions

Domain

26 – 291

266

Tyrosine-protein phosphatase

Region

229 – 235

7

Substrate binding By similarity

Sites

Active site

229

1

Phosphocysteine intermediate By similarity

Binding site

197

1

Substrate By similarity

Binding site

276

1

Substrate By similarity

Amino acid modifications

Modified residue

10

1

Phosphoserine Ref.5

Modified residue

389

1

Phosphotyrosine Ref.4 Ref.6 Ref.7 Ref.8

Modified residue

426

1

Phosphotyrosine By similarity

Natural variations

Natural variant

193

1

M → V.
Corresponds to variant rs3739124 [ dbSNP | Ensembl ].

VAR_047651

Experimental info

Sequence conflict

356 – 357

2

VV → EE in CAA56105. Ref.1

Sequence conflict

378 – 379

2

Missing in CAA56105. Ref.1

Secondary structure

1

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460

Helix Strand Turn

Details...

Helix

11 – 14

4

Helix

25 – 43

19

Helix

49 – 52

4

Turn

54 – 56

3

Helix

57 – 59

3

Helix

69 – 71

3

Beta strand

72 – 74

3

Helix

79 – 81

3

Beta strand

85 – 93

9

Beta strand

97 – 104

8

Helix

109 – 111

3

Helix

112 – 121

10

Beta strand

126 – 129

4

Beta strand

133 – 135

3

Beta strand

152 – 154

3

Beta strand

157 – 168

12

Beta strand

171 – 180

10

Beta strand

183 – 192

10

Beta strand

197 – 199

3

Helix

205 – 218

14

Beta strand

225 – 228

4

Beta strand

230 – 233

4

Helix

234 – 250

17

Helix

260 – 268

9

Helix

278 – 292

15

Sequences

	Sequence		Length	Mass (Da)
	Q99952 [UniParc]. Last modified November 25, 2008. Version 2. Checksum: 67ED24A0504D1883	FASTA	460	50,482
	10 20 30 40 50 60 MSRSLDSARS FLERLEARGG REGAVLAGEF SDIQACSAAW KADGVCSTVA GSRPENVRKN 70 80 90 100 110 120 RYKDVLPYDQ TRVILSLLQE EGHSDYINGN FIRGVDGSLA YIATQGPLPH TLLDFWRLVW 130 140 150 160 170 180 EFGVKVILMA CREIENGRKR CERYWAQEQE PLQTGLFCIT LIKEKWLNED IMLRTLKVTF 190 200 210 220 230 240 QKESRSVYQL QYMSWPDRGV PSSPDHMLAM VEEARRLQGS GPEPLCVHCS AGCGRTGVLC 250 260 270 280 290 300 TVDYVRQLLL TQMIPPDFSL FDVVLKMRKQ RPAAVQTEEQ YRFLYHTVAQ MFCSTLQNAS 310 320 330 340 350 360 PHYQNIKENC APLYDDALFL RTPQALLAIP RPPGGVLRSI SVPGSPGHAM ADTYAVVQKR 370 380 390 400 410 420 GAPAGAGSGT QTGTGTGTGA RSAEEAPLYS KVTPRAQRPG AHAEDARGTL PGRVPADQSP 430 440 450 460 AGSGAYEDVA GGAQTGGLGF NLRIGRPKGP RDPPAEWTRV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the PEST family protein tyrosine phosphatase BDP1." Kim Y.W., Wang H.-Y., Sures I., Lammers R., Martell K.J., Ullrich A. Oncogene 13:2275-2279(1996) [PubMed: 8950995] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, CHARACTERIZATION. Tissue: Brain.
[2]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Time-resolved mass spectrometry of tyrosine phosphorylation sites in the epidermal growth factor receptor signaling network reveals dynamic modules." Zhang Y., Wolf-Yadlin A., Ross P.L., Pappin D.J., Rush J., Lauffenburger D.A., White F.M. Mol. Cell. Proteomics 4:1240-1250(2005) [PubMed: 15951569] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-389, MASS SPECTROMETRY. Tissue: Mammary epithelium.
[5]	"Quantitative phosphoproteome analysis using a dendrimer conjugation chemistry and tandem mass spectrometry." Tao W.A., Wollscheid B., O'Brien R., Eng J.K., Li X.-J., Bodenmiller B., Watts J.D., Hood L., Aebersold R. Nat. Methods 2:591-598(2005) [PubMed: 16094384] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[6]	"Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer." Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J., Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L., Mitchell J., Wetzel R., Macneill J., Ren J.M. , Yuan J., Bakalarski C.E., Villen J., Kornhauser J.M., Smith B., Li D., Zhou X., Gygi S.P., Gu T.-L., Polakiewicz R.D., Rush J., Comb M.J. Cell 131:1190-1203(2007) [PubMed: 18083107] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-389, MASS SPECTROMETRY. Tissue: Lung carcinoma.
[7]	"Multiple reaction monitoring for robust quantitative proteomic analysis of cellular signaling networks." Wolf-Yadlin A., Hautaniemi S., Lauffenburger D.A., White F.M. Proc. Natl. Acad. Sci. U.S.A. 104:5860-5865(2007) [PubMed: 17389395] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-389, MASS SPECTROMETRY. Tissue: Mammary epithelium.
[8]	"An extensive survey of tyrosine phosphorylation revealing new sites in human mammary epithelial cells." Heibeck T.H., Ding S.-J., Opresko L.K., Zhao R., Schepmoes A.A., Yang F., Tolmachev A.V., Monroe M.E., Camp D.G. II, Smith R.D., Wiley H.S., Qian W.-J. J. Proteome Res. 8:3852-3861(2009) [PubMed: 19534553] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-389, MASS SPECTROMETRY. Tissue: Mammary epithelium.
[9]	"Large-scale structural analysis of the classical human protein tyrosine phosphatome." Barr A.J., Ugochukwu E., Lee W.H., King O.N.F., Filippakopoulos P., Alfano I., Savitsky P., Burgess-Brown N.A., Mueller S., Knapp S. Cell 136:352-363(2009) [PubMed: 19167335] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 6-299.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X79568 mRNA. Translation: CAA56105.1.
AC132479 Genomic DNA. Translation: AAY24077.1.
CH471263 Genomic DNA. Translation: EAW55619.1.

IPI

IPI00219132.

RefSeq

NP_055184.2. NM_014369.3.

UniGene

Hs.516390.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2OC3	X-ray	1.50	A	6-299	[»]

ProteinModelPortal

Q99952.

SMR

Q99952. Positions 6-293.

ModBase

Search...

Protein-protein interaction databases

STRING

Q99952.

PTM databases

PhosphoSite

Q99952.

Polymorphism databases

DMDM

215273871.

Proteomic databases

PRIDE

Q99952.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000175756; ENSP00000175756; ENSG00000072135.

GeneID

26469.

KEGG

hsa:26469.

Organism-specific databases

CTD

26469.

GeneCards

GC02P131113.

H-InvDB

HIX0023989.

HGNC

HGNC:9649. PTPN18.

HPA

CAB012174.

MIM

606587. gene.

neXtProt

NX_Q99952.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG05559.

GeneTree

ENSGT00600000084181.

HOGENOM

HBG717310.

HOVERGEN

HBG053419.

InParanoid

Q99952.

OMA

MADTYAV.

OrthoDB

EOG4X0MSD.

PhylomeDB

Q99952.

Gene expression databases

ArrayExpress

Q99952.

Bgee

Q99952.

CleanEx

HS_BDP1.
HS_PTPN18.

Genevestigator

Q99952.

GermOnline

ENSG00000072135. Homo sapiens.

Family and domain databases

InterPro

IPR000387. Tyr/Dual-specificity_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
[Graphical view]

KO

K01104.

Pfam

PF00102. Y_phosphatase. 1 hit.
[Graphical view]

PRINTS

PR00700. PRTYPHPHTASE.

SMART

SM00194. PTPc. 1 hit.
[Graphical view]

PROSITE

PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

48711.

SOURCE

Search...

Entry information

Entry name

PTN18_HUMAN

Accession

Primary (citable) accession number: Q99952
Secondary accession number(s): Q53P42

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	November 25, 2008
Last modified:	January 25, 2012
This is version 103 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.