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Protein

1-acyl-sn-glycerol-3-phosphate acyltransferase alpha

Gene

AGPAT1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone.1 Publication

Catalytic activityi

Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate.1 Publication

Pathwayi: CDP-diacylglycerol biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glycerol-3-phosphate acyltransferase 2, mitochondrial (GPAT2), Glycerol-3-phosphate acyltransferase 1, mitochondrial (GPAM), Glycerol-3-phosphate acyltransferase 1, mitochondrial (GPAM), Glycerol-3-phosphate acyltransferase 4 (GPAT4), Glycerol-3-phosphate acyltransferase 3 (GPAT3), Glycerol-3-phosphate acyltransferase 1, mitochondrial, Glycerol-3-phosphate acyltransferase 1, mitochondrial (GPAM), Glycerol-3-phosphate acyltransferase 1, mitochondrial (DKFZp451B1115)
  2. 1-acyl-sn-glycerol-3-phosphate acyltransferase beta (AGPAT2), 1-acyl-sn-glycerol-3-phosphate acyltransferase delta (AGPAT4), 1-acyl-sn-glycerol-3-phosphate acyltransferase gamma (AGPAT3), 1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon (AGPAT5), 1-acyl-sn-glycerol-3-phosphate acyltransferase alpha (AGPAT1), Lysocardiolipin acyltransferase 1 (LCLAT1)
  3. Phosphatidate cytidylyltransferase 1 (CDS1), Phosphatidate cytidylyltransferase 2 (CDS2), Phosphatidate cytidylyltransferase, mitochondrial (TAMM41), Phosphatidate cytidylyltransferase (CDS1), Phosphatidate cytidylyltransferase, Phosphatidate cytidylyltransferase, Phosphatidate cytidylyltransferase, Phosphatidate cytidylyltransferase
This subpathway is part of the pathway CDP-diacylglycerol biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate, the pathway CDP-diacylglycerol biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

  • 1-acylglycerol-3-phosphate O-acyltransferase activity Source: BHF-UCL

GO - Biological processi

  • CDP-diacylglycerol biosynthetic process Source: UniProtKB-UniPathway
  • phosphatidic acid biosynthetic process Source: BHF-UCL
  • phospholipid metabolic process Source: ProtInc
  • positive regulation of cellular metabolic process Source: Reactome
  • positive regulation of cytokine-mediated signaling pathway Source: BHF-UCL
  • positive regulation of cytokine production Source: BHF-UCL

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processLipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:HS09762-MONOMER
BRENDAi2.3.1.51 2681
ReactomeiR-HSA-1483166 Synthesis of PA
R-HSA-163765 ChREBP activates metabolic gene expression
UniPathwayiUPA00557; UER00613

Chemistry databases

SwissLipidsiSLP:000000095

Names & Taxonomyi

Protein namesi
Recommended name:
1-acyl-sn-glycerol-3-phosphate acyltransferase alpha (EC:2.3.1.51)
Alternative name(s):
1-acylglycerol-3-phosphate O-acyltransferase 1
Short name:
1-AGP acyltransferase 1
Short name:
1-AGPAT 1
Lysophosphatidic acid acyltransferase alpha
Short name:
LPAAT-alpha
Protein G15
Gene namesi
Name:AGPAT1
Synonyms:G15
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000204310.10
HGNCiHGNC:324 AGPAT1
MIMi603099 gene
neXtProtiNX_Q99943

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei38 – 58HelicalSequence analysisAdd BLAST21
Transmembranei128 – 148HelicalSequence analysisAdd BLAST21
Transmembranei193 – 213HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi10554
OpenTargetsiENSG00000204310
PharmGKBiPA24621

Chemistry databases

ChEMBLiCHEMBL3583

Polymorphism and mutation databases

BioMutaiAGPAT1
DMDMi3914372

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26Sequence analysisAdd BLAST26
ChainiPRO_000020819027 – 2831-acyl-sn-glycerol-3-phosphate acyltransferase alphaAdd BLAST257

Proteomic databases

EPDiQ99943
MaxQBiQ99943
PaxDbiQ99943
PeptideAtlasiQ99943
PRIDEiQ99943

PTM databases

iPTMnetiQ99943
PhosphoSitePlusiQ99943
SwissPalmiQ99943

Expressioni

Tissue specificityi

Widely expressed. Expressed in adipose tissue and at high levels in testis and pancreas. Expressed at lower levels in tissues such as heart, brain, placenta, kidney, lung, spleen, thymus, prostate, ovary, intestine, colon, leukocyte and liver.1 Publication

Gene expression databases

BgeeiENSG00000204310
CleanExiHS_AGPAT1
ExpressionAtlasiQ99943 baseline and differential
GenevisibleiQ99943 HS

Organism-specific databases

HPAiHPA048478
HPA073355

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
MEOX2P502223EBI-3893468,EBI-748397

Protein-protein interaction databases

BioGridi11580514 interactors.
IntActiQ99943 5 interactors.
STRINGi9606.ENSP00000337463

Structurei

3D structure databases

ProteinModelPortaliQ99943
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi104 – 109HXXXXD motif6
Motifi178 – 181EGTR motif4

Domaini

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.By similarity

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2848 Eukaryota
COG0204 LUCA
GeneTreeiENSGT00390000008726
HOGENOMiHOG000026375
HOVERGENiHBG000676
InParanoidiQ99943
KOiK13509
OMAiFHMAIDQ
OrthoDBiEOG091G0ICC
PhylomeDBiQ99943
TreeFamiTF314867

Family and domain databases

InterProiView protein in InterPro
IPR004552 AGP_acyltrans
IPR002123 Plipid/glycerol_acylTrfase
PfamiView protein in Pfam
PF01553 Acyltransferase, 1 hit
SMARTiView protein in SMART
SM00563 PlsC, 1 hit
TIGRFAMsiTIGR00530 AGP_acyltrn, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99943-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLWPGAWML LLLLFLLLLF LLPTLWFCSP SAKYFFKMAF YNGWILFLAV
60 70 80 90 100
LAIPVCAVRG RNVENMKILR LMLLHIKYLY GIRVEVRGAH HFPPSQPYVV
110 120 130 140 150
VSNHQSSLDL LGMMEVLPGR CVPIAKRELL WAGSAGLACW LAGVIFIDRK
160 170 180 190 200
RTGDAISVMS EVAQTLLTQD VRVWVFPEGT RNHNGSMLPF KRGAFHLAVQ
210 220 230 240 250
AQVPIVPIVM SSYQDFYCKK ERRFTSGQCQ VRVLPPVPTE GLTPDDVPAL
260 270 280
ADRVRHSMLT VFREISTDGR GGGDYLKKPG GGG
Length:283
Mass (Da):31,717
Last modified:January 1, 1998 - v2
Checksum:i71F3207259747C68
GO

Sequence cautioni

The sequence AAB47493 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05059330P → S. Corresponds to variant dbSNP:rs11964847Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U56417 mRNA Translation: AAB58775.1
U75971 mRNA Translation: AAB96378.1
Y09565 mRNA Translation: CAA70758.1
U89336 Genomic DNA Translation: AAB47493.1 Different initiation.
AL662884 Genomic DNA No translation available.
AL662828 Genomic DNA No translation available.
AL845464 Genomic DNA No translation available.
BX284686 Genomic DNA No translation available.
BX927239 Genomic DNA No translation available.
CR933878 Genomic DNA No translation available.
CR812478 Genomic DNA No translation available.
CH471081 Genomic DNA Translation: EAX03600.1
BC002402 mRNA Translation: AAH02402.1
BC003007 mRNA Translation: AAH03007.1
BC004310 mRNA Translation: AAH04310.1
BC090849 mRNA Translation: AAH90849.1
CCDSiCCDS4744.1
RefSeqiNP_006402.1, NM_006411.3
NP_116130.2, NM_032741.4
XP_005248863.1, XM_005248806.2
XP_011512536.1, XM_011514234.1
UniGeneiHs.409230

Genome annotation databases

EnsembliENST00000336984; ENSP00000337463; ENSG00000204310
ENST00000375104; ENSP00000364245; ENSG00000204310
ENST00000375107; ENSP00000364248; ENSG00000204310
ENST00000383294; ENSP00000372782; ENSG00000206324
ENST00000395496; ENSP00000378874; ENSG00000204310
ENST00000395497; ENSP00000378875; ENSG00000204310
ENST00000395499; ENSP00000378877; ENSG00000204310
ENST00000399825; ENSP00000382721; ENSG00000206324
ENST00000399827; ENSP00000382723; ENSG00000206324
ENST00000399829; ENSP00000382725; ENSG00000206324
ENST00000399830; ENSP00000382726; ENSG00000206324
ENST00000399833; ENSP00000382728; ENSG00000206324
ENST00000414520; ENSP00000406615; ENSG00000227642
ENST00000414933; ENSP00000388870; ENSG00000227642
ENST00000415173; ENSP00000412805; ENSG00000228892
ENST00000416363; ENSP00000407189; ENSG00000236873
ENST00000417388; ENSP00000415015; ENSG00000228892
ENST00000424030; ENSP00000397765; ENSG00000236873
ENST00000425204; ENSP00000388253; ENSG00000226467
ENST00000425572; ENSP00000394919; ENSG00000235758
ENST00000427763; ENSP00000401410; ENSG00000226467
ENST00000430226; ENSP00000412304; ENSG00000235758
ENST00000430777; ENSP00000392820; ENSG00000236873
ENST00000433376; ENSP00000387810; ENSG00000228892
ENST00000433896; ENSP00000408842; ENSG00000236873
ENST00000434614; ENSP00000401287; ENSG00000235758
ENST00000436149; ENSP00000388090; ENSG00000235758
ENST00000439774; ENSP00000398016; ENSG00000236873
ENST00000440840; ENSP00000395863; ENSG00000236873
ENST00000444369; ENSP00000408032; ENSG00000227642
ENST00000446323; ENSP00000392083; ENSG00000226467
ENST00000446463; ENSP00000405864; ENSG00000226467
ENST00000448196; ENSP00000404106; ENSG00000235758
ENST00000449776; ENSP00000410831; ENSG00000226467
ENST00000451833; ENSP00000406582; ENSG00000228892
ENST00000452393; ENSP00000406614; ENSG00000228892
ENST00000452427; ENSP00000387738; ENSG00000235758
ENST00000454929; ENSP00000390988; ENSG00000227642
ENST00000455898; ENSP00000395613; ENSG00000228892
ENST00000456421; ENSP00000389516; ENSG00000227642
ENST00000456679; ENSP00000388815; ENSG00000227642
ENST00000457923; ENSP00000405565; ENSG00000226467
GeneIDi10554
KEGGihsa:10554
UCSCiuc003oae.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiPLCA_HUMAN
AccessioniPrimary (citable) accession number: Q99943
Secondary accession number(s): A2BFI5, Q5BL03
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: April 25, 2018
This is version 161 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome