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Q99943 (PLCA_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 128. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1-acyl-sn-glycerol-3-phosphate acyltransferase alpha

EC=2.3.1.51
Alternative name(s):
1-acylglycerol-3-phosphate O-acyltransferase 1
Short name=1-AGP acyltransferase 1
Short name=1-AGPAT 1
Lysophosphatidic acid acyltransferase alpha
Short name=LPAAT-alpha
Protein G15
Gene names
Name:AGPAT1
Synonyms:G15
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length283 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. Ref.8

Catalytic activity

Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate. Ref.8

Pathway

Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 2/3.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.8.

Tissue specificity

Widely expressed. Expressed in adipose tissue and at high levels in testis and pancreas. Expressed at lower levels in tissues such as heart, brain, placenta, kidney, lung, spleen, thymus, prostate, ovary, intestine, colon, leukocyte and liver. Ref.8

Domain

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.

Sequence similarities

Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.

Sequence caution

The sequence AAB47493.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
   Biological processLipid biosynthesis
Lipid metabolism
Phospholipid biosynthesis
Phospholipid metabolism
   Cellular componentEndoplasmic reticulum
Membrane
   Coding sequence diversityPolymorphism
   DomainSignal
Transmembrane
Transmembrane helix
   Molecular functionAcyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processCDP-diacylglycerol biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

cellular lipid metabolic process

Traceable author statement. Source: Reactome

energy reserve metabolic process

Traceable author statement. Source: Reactome

glycerophospholipid biosynthetic process

Traceable author statement. Source: Reactome

phosphatidic acid biosynthetic process

Inferred from genetic interaction Ref.1. Source: BHF-UCL

phospholipid metabolic process

Traceable author statement. Source: Reactome

positive regulation of cellular metabolic process

Traceable author statement. Source: Reactome

positive regulation of cytokine production

Inferred from mutant phenotype Ref.1. Source: BHF-UCL

positive regulation of cytokine-mediated signaling pathway

Inferred by curator Ref.1. Source: BHF-UCL

small molecule metabolic process

Traceable author statement. Source: Reactome

triglyceride biosynthetic process

Traceable author statement. Source: Reactome

   Cellular_componentendoplasmic reticulum

Traceable author statement Ref.3. Source: ProtInc

endoplasmic reticulum membrane

Traceable author statement. Source: Reactome

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_function1-acylglycerol-3-phosphate O-acyltransferase activity

Inferred from genetic interaction Ref.1. Source: BHF-UCL

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Potential
Chain27 – 2832571-acyl-sn-glycerol-3-phosphate acyltransferase alpha
PRO_0000208190

Regions

Transmembrane38 – 5821Helical; Potential
Transmembrane128 – 14821Helical; Potential
Transmembrane193 – 21321Helical; Potential
Motif104 – 1096HXXXXD motif
Motif178 – 1814EGTR motif

Natural variations

Natural variant301P → S.
Corresponds to variant rs11964847 [ dbSNP | Ensembl ].
VAR_050593

Sequences

Sequence LengthMass (Da)Tools
Q99943 [UniParc].

Last modified January 1, 1998. Version 2.
Checksum: 71F3207259747C68

FASTA28331,717
        10         20         30         40         50         60 
MDLWPGAWML LLLLFLLLLF LLPTLWFCSP SAKYFFKMAF YNGWILFLAV LAIPVCAVRG 

        70         80         90        100        110        120 
RNVENMKILR LMLLHIKYLY GIRVEVRGAH HFPPSQPYVV VSNHQSSLDL LGMMEVLPGR 

       130        140        150        160        170        180 
CVPIAKRELL WAGSAGLACW LAGVIFIDRK RTGDAISVMS EVAQTLLTQD VRVWVFPEGT 

       190        200        210        220        230        240 
RNHNGSMLPF KRGAFHLAVQ AQVPIVPIVM SSYQDFYCKK ERRFTSGQCQ VRVLPPVPTE 

       250        260        270        280 
GLTPDDVPAL ADRVRHSMLT VFREISTDGR GGGDYLKKPG GGG 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and expression of two human lysophosphatidic acid acyltransferase cDNAs that enhance cytokine-induced signaling responses in cells."
West J., Tompkins C.K., Balantac N., Nudelman E., Meengs B., White T., Bursten S., Coleman J., Kumar A., Singer J.W., Leung D.W.
DNA Cell Biol. 16:691-701(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"A human cDNA sequence with homology to non-mammalian lysophosphatidic acid acyltransferases."
Stamps A.C., Elmore M.A., Hill M.E., Kelly K., Makda A.A., Finnen M.J.
Biochem. J. 326:455-461(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Characterization of a human lysophosphatidic acid acyltransferase that is encoded by a gene located in the class III region of the human major histocompatibility complex."
Aguado B., Campbell R.D.
J. Biol. Chem. 273:4096-4105(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"Analysis of the gene-dense major histocompatibility complex class III region and its comparison to mouse."
Xie T., Rowen L., Aguado B., Ahearn M.E., Madan A., Qin S., Campbell R.D., Hood L.
Genome Res. 13:2621-2636(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The DNA sequence and analysis of human chromosome 6."
Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain, Lung and Skin.
[8]"Human 1-acylglycerol-3-phosphate O-acyltransferase isoforms 1 and 2: biochemical characterization and inability to rescue hepatic steatosis in Agpat2(-/-) gene lipodystrophic mice."
Agarwal A.K., Sukumaran S., Cortes V.A., Tunison K., Mizrachi D., Sankella S., Gerard R.D., Horton J.D., Garg A.
J. Biol. Chem. 286:37676-37691(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U56417 mRNA. Translation: AAB58775.1.
U75971 mRNA. Translation: AAB96378.1.
Y09565 mRNA. Translation: CAA70758.1.
U89336 Genomic DNA. Translation: AAB47493.1. Different initiation.
AL662884 Genomic DNA. Translation: CAI18347.1.
AL662828 Genomic DNA. Translation: CAI17430.1.
AL845464 Genomic DNA. Translation: CAI41807.1.
BX284686 Genomic DNA. Translation: CAM26221.1.
BX927239 Genomic DNA. Translation: CAQ06593.1.
CR933878 Genomic DNA. Translation: CAQ09622.1.
CR812478 Genomic DNA. Translation: CAQ10697.1.
CH471081 Genomic DNA. Translation: EAX03600.1.
BC002402 mRNA. Translation: AAH02402.1.
BC003007 mRNA. Translation: AAH03007.1.
BC004310 mRNA. Translation: AAH04310.1.
BC090849 mRNA. Translation: AAH90849.1.
RefSeqNP_006402.1. NM_006411.3.
NP_116130.2. NM_032741.4.
XP_005248863.1. XM_005248806.1.
XP_005272819.1. XM_005272762.1.
XP_005274887.1. XM_005274830.1.
XP_005275131.1. XM_005275074.1.
XP_005275261.1. XM_005275204.1.
XP_005275395.1. XM_005275338.1.
XP_005275562.1. XM_005275505.1.
UniGeneHs.409230.

3D structure databases

ProteinModelPortalQ99943.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115805. 6 interactions.
IntActQ99943. 2 interactions.
STRING9606.ENSP00000401287.

Chemistry

ChEMBLCHEMBL3583.

PTM databases

PhosphoSiteQ99943.

Polymorphism databases

DMDM3914372.

Proteomic databases

PaxDbQ99943.
PRIDEQ99943.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000336984; ENSP00000337463; ENSG00000204310.
ENST00000375104; ENSP00000364245; ENSG00000204310.
ENST00000375107; ENSP00000364248; ENSG00000204310.
ENST00000383294; ENSP00000372782; ENSG00000206324.
ENST00000395496; ENSP00000378874; ENSG00000204310.
ENST00000395497; ENSP00000378875; ENSG00000204310.
ENST00000395499; ENSP00000378877; ENSG00000204310.
ENST00000399825; ENSP00000382721; ENSG00000206324.
ENST00000399827; ENSP00000382723; ENSG00000206324.
ENST00000399829; ENSP00000382725; ENSG00000206324.
ENST00000399830; ENSP00000382726; ENSG00000206324.
ENST00000399833; ENSP00000382728; ENSG00000206324.
ENST00000414520; ENSP00000406615; ENSG00000227642.
ENST00000414933; ENSP00000388870; ENSG00000227642.
ENST00000415173; ENSP00000412805; ENSG00000228892.
ENST00000416363; ENSP00000407189; ENSG00000236873.
ENST00000417388; ENSP00000415015; ENSG00000228892.
ENST00000424030; ENSP00000397765; ENSG00000236873.
ENST00000425204; ENSP00000388253; ENSG00000226467.
ENST00000425572; ENSP00000394919; ENSG00000235758.
ENST00000427763; ENSP00000401410; ENSG00000226467.
ENST00000430226; ENSP00000412304; ENSG00000235758.
ENST00000430777; ENSP00000392820; ENSG00000236873.
ENST00000433376; ENSP00000387810; ENSG00000228892.
ENST00000433896; ENSP00000408842; ENSG00000236873.
ENST00000434614; ENSP00000401287; ENSG00000235758.
ENST00000436149; ENSP00000388090; ENSG00000235758.
ENST00000439774; ENSP00000398016; ENSG00000236873.
ENST00000440840; ENSP00000395863; ENSG00000236873.
ENST00000444369; ENSP00000408032; ENSG00000227642.
ENST00000446323; ENSP00000392083; ENSG00000226467.
ENST00000446463; ENSP00000405864; ENSG00000226467.
ENST00000448196; ENSP00000404106; ENSG00000235758.
ENST00000449776; ENSP00000410831; ENSG00000226467.
ENST00000451833; ENSP00000406582; ENSG00000228892.
ENST00000452393; ENSP00000406614; ENSG00000228892.
ENST00000452427; ENSP00000387738; ENSG00000235758.
ENST00000454929; ENSP00000390988; ENSG00000227642.
ENST00000455898; ENSP00000395613; ENSG00000228892.
ENST00000456421; ENSP00000389516; ENSG00000227642.
ENST00000456679; ENSP00000388815; ENSG00000227642.
ENST00000457923; ENSP00000405565; ENSG00000226467.
GeneID10554.
KEGGhsa:10554.
UCSCuc003oae.3. human.

Organism-specific databases

CTD10554.
GeneCardsGC06M032135.
GC06Mi32147.
GC06Mj32084.
GC06Ml32175.
GC06Mm32212.
GC06Mn32093.
GC06Mo32142.
HGNCHGNC:324. AGPAT1.
MIM603099. gene.
neXtProtNX_Q99943.
PharmGKBPA24621.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0204.
HOGENOMHOG000026375.
HOVERGENHBG000676.
InParanoidQ99943.
KOK13509.
OMAGVVIIEM.
PhylomeDBQ99943.
TreeFamTF314867.

Enzyme and pathway databases

BioCycMetaCyc:HS09762-MONOMER.
BRENDA2.3.1.51. 2681.
ReactomeREACT_111217. Metabolism.
UniPathwayUPA00557; UER00613.

Gene expression databases

ArrayExpressQ99943.
BgeeQ99943.
CleanExHS_AGPAT1.
GenevestigatorQ99943.

Family and domain databases

InterProIPR004552. AGP_acyltrans.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PfamPF01553. Acyltransferase. 1 hit.
[Graphical view]
SMARTSM00563. PlsC. 1 hit.
[Graphical view]
TIGRFAMsTIGR00530. AGP_acyltrn. 1 hit.
ProtoNetSearch...

Other

GeneWikiAGPAT1.
GenomeRNAi10554.
NextBio40041.
PROQ99943.
SOURCESearch...

Entry information

Entry namePLCA_HUMAN
AccessionPrimary (citable) accession number: Q99943
Secondary accession number(s): A2BFI5, Q5BL03
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: April 16, 2014
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 6

Human chromosome 6: entries, gene names and cross-references to MIM