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Protein

E3 ubiquitin-protein ligase RNF5

Gene

RNF5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has E2-dependent E3 ubiquitin-protein ligase activity. May function together with E2 ubiquitin-conjugating enzymes UBE2D1/UBCH5A and UBE2D2/UBC4. Mediates ubiquitination of PXN/paxillin and Salmonella type III secreted protein sopA. May be involved in regulation of cell motility and localization of PXN/paxillin. Mediates the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD; the ubiquitination appears to involve E2 ubiquitin-conjugating enzyme UBE2N. Mediates the 'Lys-48'-linked polyubiquitination of TMEM173 at 'Lys-150' leading to its proteasomal degradation; the ubiquitination occurrs in mitochondria after viral transfection and regulates antiviral responses.5 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri27 – 68RING-typePROSITE-ProRule annotationAdd BLAST42

GO - Molecular functioni

  • ligase activity Source: UniProtKB-KW
  • ubiquitin-like protein conjugating enzyme binding Source: GO_Central
  • ubiquitin protein ligase activity involved in ERAD pathway Source: ParkinsonsUK-UCL
  • ubiquitin-protein transferase activity Source: UniProtKB
  • zinc ion binding Source: ProtInc

GO - Biological processi

  • cellular protein catabolic process Source: UniProtKB
  • ERAD pathway Source: ParkinsonsUK-UCL
  • ER-associated misfolded protein catabolic process Source: UniProtKB
  • negative regulation of autophagy Source: Ensembl
  • protein destabilization Source: Ensembl
  • protein K48-linked ubiquitination Source: UniProtKB
  • protein K63-linked ubiquitination Source: UniProtKB
  • protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: GO_Central
  • regulation of autophagosome assembly Source: Ensembl
  • response to bacterium Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:ENSG00000096678-MONOMER.
ReactomeiR-HSA-382556. ABC-family proteins mediated transport.
R-HSA-5678895. Defective CFTR causes cystic fibrosis.
R-HSA-901032. ER Quality Control Compartment (ERQC).
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RNF5 (EC:6.3.2.-)
Alternative name(s):
Protein G16
RING finger protein 5
Ram1 homolog
Short name:
HsRma1
Gene namesi
Name:RNF5
Synonyms:G16, NG2, RMA1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:10068. RNF5.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei118 – 138HelicalSequence analysisAdd BLAST21
Transmembranei160 – 180HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi42C → S: Loss of E3 ubiquitin-protein ligase activity. 1 Publication1

Organism-specific databases

DisGeNETi6048.
OpenTargetsiENSG00000183574.
ENSG00000204308.
ENSG00000223767.
ENSG00000225452.
ENSG00000227277.
ENSG00000228405.
ENSG00000228907.
PharmGKBiPA34442.

Polymorphism and mutation databases

BioMutaiRNF5.
DMDMi74762702.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002403932 – 180E3 ubiquitin-protein ligase RNF5Add BLAST179

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei84PhosphoserineCombined sources1
Modified residuei94PhosphothreonineCombined sources1
Modified residuei107PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ99942.
MaxQBiQ99942.
PaxDbiQ99942.
PeptideAtlasiQ99942.
PRIDEiQ99942.
TopDownProteomicsiQ99942.

PTM databases

iPTMnetiQ99942.
PhosphoSitePlusiQ99942.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiENSG00000183574.
CleanExiHS_RNF5.
ExpressionAtlasiQ99942. baseline and differential.
GenevisibleiQ99942. HS.

Organism-specific databases

HPAiCAB034107.

Interactioni

Subunit structurei

Interacts with PXN. Interacts with Salmonella typhimurium sopA. Interacts with JKAMP. Interacts with TMEM173; the interaction of endogenous proteins is dependent on viral infection.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ABHD16AO958704EBI-348482,EBI-348517
CFTRP135693EBI-348482,EBI-349854
CYB561P494473EBI-348482,EBI-8646596
CYB561A3Q8NBI25EBI-348482,EBI-10269179
INSIG2Q9Y5U45EBI-348482,EBI-8503746
PXNP490236EBI-348482,EBI-702209
RNF185Q96GF13EBI-348482,EBI-2340249
SEC22AQ96IW73EBI-348482,EBI-8652744
SLC1A1P430053EBI-348482,EBI-745376
SLC1A5Q157584EBI-348482,EBI-356576
SLC38A2Q96QD83EBI-348482,EBI-723083
SLC38A7Q9NVC33EBI-348482,EBI-10314552
TMEM242Q9NWH23EBI-348482,EBI-10315004
UBE2D1P516687EBI-348482,EBI-743540
UBE2D2P628375EBI-348482,EBI-347677
UBE2D3P610778EBI-348482,EBI-348268
UBE2D4Q9Y2X86EBI-348482,EBI-745527
UBE2E2Q96LR57EBI-348482,EBI-2129763
UBE2E3Q969T47EBI-348482,EBI-348496
UBE2KP610865EBI-348482,EBI-473850
UBE2WQ96B024EBI-348482,EBI-716589
UBE2WQ96FI03EBI-348482,EBI-10285774
YIPF2Q9BWQ63EBI-348482,EBI-751204

GO - Molecular functioni

Protein-protein interaction databases

BioGridi111975. 52 interactors.
DIPiDIP-29268N.
IntActiQ99942. 50 interactors.
MINTiMINT-1032027.
STRINGi9606.ENSP00000364235.

Structurei

3D structure databases

ProteinModelPortaliQ99942.
SMRiQ99942.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri27 – 68RING-typePROSITE-ProRule annotationAdd BLAST42

Keywords - Domaini

Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiKOG0823. Eukaryota.
ENOG4111IHV. LUCA.
GeneTreeiENSGT00390000014107.
HOGENOMiHOG000238304.
HOVERGENiHBG054495.
InParanoidiQ99942.
KOiK10666.
OMAiFQSFGDT.
OrthoDBiEOG091G0W2I.
PhylomeDBiQ99942.
TreeFamiTF317334.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99942-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAEEEDGG PEGPNRERGG AGATFECNIC LETAREAVVS VCGHLYCWPC
60 70 80 90 100
LHQWLETRPE RQECPVCKAG ISREKVVPLY GRGSQKPQDP RLKTPPRPQG
110 120 130 140 150
QRPAPESRGG FQPFGDTGGF HFSFGVGAFP FGFFTTVFNA HEPFRRGTGV
160 170 180
DLGQGHPASS WQDSLFLFLA IFFFFWLLSI
Length:180
Mass (Da):19,881
Last modified:May 1, 1997 - v1
Checksum:iE5AFA4DE6DE85942
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti22G → S in CAB51286 (Ref. 3) Curated1
Sequence conflicti60E → D in CAB51286 (Ref. 3) Curated1
Sequence conflicti117T → A in CAB51286 (Ref. 3) Curated1
Sequence conflicti148T → A in CAB51286 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB056869 mRNA. Translation: BAB39359.1.
AJ243936 mRNA. Translation: CAB51286.1.
AK311859 mRNA. Translation: BAG34800.1.
BT007105 mRNA. Translation: AAP35769.1.
U89336 Genomic DNA. Translation: AAB47492.1.
AL845464 Genomic DNA. Translation: CAI41808.1.
AL662884 Genomic DNA. Translation: CAI18351.1.
AL662830 Genomic DNA. Translation: CAI17532.1.
BX284686 Genomic DNA. Translation: CAM26222.1.
BX927239 Genomic DNA. Translation: CAQ06594.1.
CR812478 Genomic DNA. Translation: CAQ10698.1.
CR933878 Genomic DNA. Translation: CAQ09623.1.
BC004155 mRNA. Translation: AAH04155.1.
BC111392 mRNA. Translation: AAI11393.1.
BC119741 mRNA. Translation: AAI19742.1.
BC119742 mRNA. Translation: AAI19743.1.
BC127651 mRNA. Translation: AAI27652.1.
BC127652 mRNA. Translation: AAI27653.1.
BC148255 mRNA. Translation: AAI48256.1.
CCDSiCCDS4745.1.
RefSeqiNP_008844.1. NM_006913.3.
UniGeneiHs.731774.

Genome annotation databases

EnsembliENST00000375094; ENSP00000364235; ENSG00000204308.
ENST00000413786; ENSP00000387879; ENSG00000225452.
ENST00000432616; ENSP00000413131; ENSG00000183574.
ENST00000445885; ENSP00000401172; ENSG00000227277.
ENST00000449794; ENSP00000415784; ENSG00000223767.
ENST00000453473; ENSP00000415127; ENSG00000228907.
ENST00000456167; ENSP00000388795; ENSG00000228405.
GeneIDi6048.
KEGGihsa:6048.
UCSCiuc003oaj.5. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB056869 mRNA. Translation: BAB39359.1.
AJ243936 mRNA. Translation: CAB51286.1.
AK311859 mRNA. Translation: BAG34800.1.
BT007105 mRNA. Translation: AAP35769.1.
U89336 Genomic DNA. Translation: AAB47492.1.
AL845464 Genomic DNA. Translation: CAI41808.1.
AL662884 Genomic DNA. Translation: CAI18351.1.
AL662830 Genomic DNA. Translation: CAI17532.1.
BX284686 Genomic DNA. Translation: CAM26222.1.
BX927239 Genomic DNA. Translation: CAQ06594.1.
CR812478 Genomic DNA. Translation: CAQ10698.1.
CR933878 Genomic DNA. Translation: CAQ09623.1.
BC004155 mRNA. Translation: AAH04155.1.
BC111392 mRNA. Translation: AAI11393.1.
BC119741 mRNA. Translation: AAI19742.1.
BC119742 mRNA. Translation: AAI19743.1.
BC127651 mRNA. Translation: AAI27652.1.
BC127652 mRNA. Translation: AAI27653.1.
BC148255 mRNA. Translation: AAI48256.1.
CCDSiCCDS4745.1.
RefSeqiNP_008844.1. NM_006913.3.
UniGeneiHs.731774.

3D structure databases

ProteinModelPortaliQ99942.
SMRiQ99942.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111975. 52 interactors.
DIPiDIP-29268N.
IntActiQ99942. 50 interactors.
MINTiMINT-1032027.
STRINGi9606.ENSP00000364235.

PTM databases

iPTMnetiQ99942.
PhosphoSitePlusiQ99942.

Polymorphism and mutation databases

BioMutaiRNF5.
DMDMi74762702.

Proteomic databases

EPDiQ99942.
MaxQBiQ99942.
PaxDbiQ99942.
PeptideAtlasiQ99942.
PRIDEiQ99942.
TopDownProteomicsiQ99942.

Protocols and materials databases

DNASUi6048.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000375094; ENSP00000364235; ENSG00000204308.
ENST00000413786; ENSP00000387879; ENSG00000225452.
ENST00000432616; ENSP00000413131; ENSG00000183574.
ENST00000445885; ENSP00000401172; ENSG00000227277.
ENST00000449794; ENSP00000415784; ENSG00000223767.
ENST00000453473; ENSP00000415127; ENSG00000228907.
ENST00000456167; ENSP00000388795; ENSG00000228405.
GeneIDi6048.
KEGGihsa:6048.
UCSCiuc003oaj.5. human.

Organism-specific databases

CTDi6048.
DisGeNETi6048.
GeneCardsiRNF5.
H-InvDBHIX0034363.
HGNCiHGNC:10068. RNF5.
HPAiCAB034107.
MIMi602677. gene.
neXtProtiNX_Q99942.
OpenTargetsiENSG00000183574.
ENSG00000204308.
ENSG00000223767.
ENSG00000225452.
ENSG00000227277.
ENSG00000228405.
ENSG00000228907.
PharmGKBiPA34442.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0823. Eukaryota.
ENOG4111IHV. LUCA.
GeneTreeiENSGT00390000014107.
HOGENOMiHOG000238304.
HOVERGENiHBG054495.
InParanoidiQ99942.
KOiK10666.
OMAiFQSFGDT.
OrthoDBiEOG091G0W2I.
PhylomeDBiQ99942.
TreeFamiTF317334.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciZFISH:ENSG00000096678-MONOMER.
ReactomeiR-HSA-382556. ABC-family proteins mediated transport.
R-HSA-5678895. Defective CFTR causes cystic fibrosis.
R-HSA-901032. ER Quality Control Compartment (ERQC).

Miscellaneous databases

GeneWikiiRNF5.
GenomeRNAii6048.
PROiQ99942.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000183574.
CleanExiHS_RNF5.
ExpressionAtlasiQ99942. baseline and differential.
GenevisibleiQ99942. HS.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRNF5_HUMAN
AccessioniPrimary (citable) accession number: Q99942
Secondary accession number(s): A2BFI6
, B2R4K3, Q0VDB7, Q9UMQ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: May 1, 1997
Last modified: November 30, 2016
This is version 156 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.