Reviewed, UniProtKB/Swiss-Prot Q99941 (ATF6B_HUMAN)

Names and origin

Protein names

Recommended name:
    Cyclic AMP-dependent transcription factor ATF-6 beta
      Short name=cAMP-dependent transcription factor ATF-6 beta
Alternative name(s):
    Activating transcription factor 6 beta
      Short name=ATF6-beta
    cAMP-responsive element-binding protein-like 1
    cAMP response element-binding protein-related protein
      Short name=Creb-rp
    Protein G13
Cleaved into the following chain:
    1- Recommended name:
            Processed cyclic AMP-dependent transcription factor ATF-6 beta

Gene names

Name:	ATF6B
Synonyms:	CREBL1, G13

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	703 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Transcriptional factor that acts in the unfolded protein response (UPR) pathway by activating UPR target genes induced during ER stress. Binds DNA on the 5'-CCAC[GA]-3' half of the ER stress response element (ERSE) (5'-CCAATN9CCAC[GA]-3') when NF-Y is bound to ERSE.
Subunit structure	Homodimer and heterodimer with ATF6-alpha. The dimer interacts with the nuclear transcription factor Y (NF-Y) trimer through direct binding to NF-Y subunit C (NF-YC).
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein. Processed cyclic AMP-dependent transcription factor ATF-6 beta: Nucleus. Note: Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus.
Tissue specificity	Ubiquitous.
Domain	The basic domain functions as a nuclear localization signal. The basic leucine-zipper domain is sufficient for association with the NF-Y trimer and binding to ERSE.
Post-translational modification	N-glycosylated. During unfolded protein response an approximative 60 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage is probably performed sequentially by site-1 and site-2 proteases.
Sequence similarities	Belongs to the bZIP family. ATF subfamily. Contains 1 bZIP domain.
Sequence caution	The sequence AAC50888.1 differs from that shown. Reason: Frameshift at position 600. The sequence AAG14898.1 differs from that shown. Reason: Frameshift at position 600.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Unfolded protein response
Cellular component	Endoplasmic reticulum Membrane Nucleus
Coding sequence diversity	Alternative splicing
Domain	Signal-anchor Transmembrane
Ligand	DNA-binding
Molecular function	Activator
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro response to unfolded protein Inferred from electronic annotation. Source: UniProtKB-KW signal transduction Ref.1 Traceable author statement. Source: ProtInc transcription Ref.1 Traceable author statement. Source: ProtInc
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Ref.1 Traceable author statement. Source: ProtInc
Molecular function	protein dimerization activity Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro transcription factor activity Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 2 (identifier: Q99941-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1 (identifier: Q99941-2)

The sequence of this isoform differs from the canonical sequence as follows:
     28-31: GLQN → D

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 703	703	Cyclic AMP-dependent transcription factor ATF-6 beta		PRO_0000076590
	Chain	1 – ?		Processed cyclic AMP-dependent transcription factor ATF-6 beta		PRO_0000296201
Regions
	Topological domain	1 – 396	396	Cytoplasmic Potential
	Transmembrane	397 – 417	21	Signal-anchor for type II membrane protein Potential
	Topological domain	418 – 703	286	Lumenal Potential
	Domain	367 – 388	22	Leucine-zipper
	DNA binding	327 – 347	21	Basic motif
	Region	1 – 86	86	Transcription activation
	Compositional bias	96 – 105	10	Poly-Ser
Sites
	Site	410	1	Important for cleavage by PS2 By similarity
	Site	413	1	Important for cleavage by PS2 By similarity
	Site	440 – 441	2	Cleavage; by PS1 By similarity
Amino acid modifications
	Glycosylation	476	1	N-linked (GlcNAc...) Potential
	Glycosylation	505	1	N-linked (GlcNAc...) Potential
	Glycosylation	610	1	N-linked (GlcNAc...) Potential
	Glycosylation	627	1	N-linked (GlcNAc...) Potential
	Glycosylation	676	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	28 – 31	4	GLQN → D in isoform 1.		VSP_000593
Experimental info
	Sequence conflict	3	1	E → D in CAA66664. Ref.2
	Sequence conflict	329 – 330	2	QQ → HE in CAA66664. Ref.2
	Sequence conflict	520	1	V → D in AAC50888. Ref.6
	Sequence conflict	600	1	D → G in AAA97438. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 2 [UniParc]. Last modified January 23, 2002. Version 2. Checksum: B04C7B23E7D83F82	FASTA	703	76,709
	10 20 30 40 50 60 MAELMLLSEI ADPTRFFTDN LLSPEDWGLQ NSTLYSGLDE VAEEQTQLFR CPEQDVPFDG 70 80 90 100 110 120 SSLDVGMDVS PSEPPWELLP IFPDLQVKSE PSSPCSSSSL SSESSRLSTE PSSEALGVGE 130 140 150 160 170 180 VLHVKTESLA PPLCLLGDDP TSSFETVQIN VIPTSDDSSD VQTKIEPVSP CSSVNSEASL 190 200 210 220 230 240 LSADSSSQAF IGEEVLEVKT ESLSPSGCLL WDVPAPSLGA VQISMGPSLD GSSGKALPTR 250 260 270 280 290 300 KPPLQPKPVV LTTVPMPSRA VPPSTTVLLQ SLVQPPPVSP VVLIQGAIRV QPEGPAPSLP 310 320 330 340 350 360 RPERKSIVPA PMPGNSCPPE VDAKLLKRQQ RMIKNRESAC QSRRKKKEYL QGLEARLQAV 370 380 390 400 410 420 LADNQQLRRE NAALRRRLEA LLAENSELKL GSGNRKVVCI MVFLLFIAFN FGPVSISEPP 430 440 450 460 470 480 SAPISPRMNK GEPQPRRHLL GFSEQEPVQG VEPLQGSSQG PKEPQPSPTD QPSFSNLTAF 490 500 510 520 530 540 PGGAKELLLR DLDQLFLSSD CRHFNRTESL RLADELSGWV QRHQRGRRKI PQRAQERQKS 550 560 570 580 590 600 QPRKKSPPVK AVPIQPPGPP ERDSVGQLQL YRHPDRSQPA FLDAIDRRED TFYVVSFRRD 610 620 630 640 650 660 HLLLPAISHN KTSRPKMSLV MPAMAPNETL SGRGAPGDYE EMMQIECEVM DTRVIHIKTS 670 680 690 700 TVPPSLRKQP SPTPGNATGG PLPVSAASQA HQASHQPLYL NHP
	Isoform 1. Checksum: 54C077BF95309216	FASTA	700	76,412
	10 20 30 40 50 60 MAELMLLSEI ADPTRFFTDN LLSPEDWDST LYSGLDEVAE EQTQLFRCPE QDVPFDGSSL 70 80 90 100 110 120 DVGMDVSPSE PPWELLPIFP DLQVKSEPSS PCSSSSLSSE SSRLSTEPSS EALGVGEVLH 130 140 150 160 170 180 VKTESLAPPL CLLGDDPTSS FETVQINVIP TSDDSSDVQT KIEPVSPCSS VNSEASLLSA 190 200 210 220 230 240 DSSSQAFIGE EVLEVKTESL SPSGCLLWDV PAPSLGAVQI SMGPSLDGSS GKALPTRKPP 250 260 270 280 290 300 LQPKPVVLTT VPMPSRAVPP STTVLLQSLV QPPPVSPVVL IQGAIRVQPE GPAPSLPRPE 310 320 330 340 350 360 RKSIVPAPMP GNSCPPEVDA KLLKRQQRMI KNRESACQSR RKKKEYLQGL EARLQAVLAD 370 380 390 400 410 420 NQQLRRENAA LRRRLEALLA ENSELKLGSG NRKVVCIMVF LLFIAFNFGP VSISEPPSAP 430 440 450 460 470 480 ISPRMNKGEP QPRRHLLGFS EQEPVQGVEP LQGSSQGPKE PQPSPTDQPS FSNLTAFPGG 490 500 510 520 530 540 AKELLLRDLD QLFLSSDCRH FNRTESLRLA DELSGWVQRH QRGRRKIPQR AQERQKSQPR 550 560 570 580 590 600 KKSPPVKAVP IQPPGPPERD SVGQLQLYRH PDRSQPAFLD AIDRREDTFY VVSFRRDHLL 610 620 630 640 650 660 LPAISHNKTS RPKMSLVMPA MAPNETLSGR GAPGDYEEMM QIECEVMDTR VIHIKTSTVP 670 680 690 700 PSLRKQPSPT PGNATGGPLP VSAASQAHQA SHQPLYLNHP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel Creb family gene telomeric of HLA-DRA in the HLA complex." Min J., Shukla H., Kozono H., Bronson S.K., Weissman S.M., Chaplin D.D. Genomics 30:149-156(1995) [PubMed: 8586413] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Tonsil.
[2]	"The gene G13 in the class III region of the human MHC encodes a potential DNA-binding protein." Khanna A., Campbell R.D. Biochem. J. 319:81-89(1996) [PubMed: 8870652] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 2).
[3]	"Analysis of the gene-dense major histocompatibility complex class III region and its comparison to mouse." Xie T., Rowen L., Aguado B., Ahearn M.E., Madan A., Qin S., Campbell R.D., Hood L. Genome Res. 13:2621-2636(2003) [PubMed: 14656967] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed: 14574404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"Alternate promoters and alternate splicing of human tenascin-X, a gene with 5' and 3' ends buried in other genes." Speek M., Barry F., Miller W.L. Hum. Mol. Genet. 5:1749-1758(1996) [PubMed: 8923003] [Abstract] Cited for: PARTIAL NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[7]	"Identification of the G13 (cAMP-response-element-binding protein-related protein) gene product related to activating transcription factor 6 as a transcriptional activator of the mammalian unfolded protein response." Haze K., Okada T., Yoshida H., Yanagi H., Yura T., Negishi M., Mori K. Biochem. J. 355:19-28(2001) [PubMed: 11256944] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U31903 mRNA. Translation: AAA97438.1. X98053 Genomic DNA. Translation: CAA66663.1. X98054 mRNA. Translation: CAA66664.1. U89337 Genomic DNA. Translation: AAB47487.1. AL049547 Genomic DNA. Translation: CAB89295.1. AL662828 Genomic DNA. Translation: CAI17416.1. AL662828 Genomic DNA. Translation: CAI17419.1. AL662884 Genomic DNA. Translation: CAI18334.1. AL662884 Genomic DNA. Translation: CAI18336.2. CR753803 Genomic DNA. Translation: CAQ09553.1. CR925796 Genomic DNA. Translation: CAQ10264.1. CH471081 Genomic DNA. Translation: EAX03581.1. U52694 Genomic DNA. Translation: AAG14900.1. U52696 mRNA. Translation: AAC50888.1. Frameshift. U52693 Genomic DNA. Translation: AAG14898.1. Frameshift. U52701 mRNA. Translation: AAC50883.1. Sequence problems.
IPI	IPI00004084. IPI00221087.
RefSeq	NP_001129625.1. NP_004372.3.
UniGene	Hs.42853
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q99941.
Proteomic databases
PRIDE	Q99941.
Genome annotation databases
Ensembl	ENST00000293709; ENSP00000293709; ENSG00000168468; Homo sapiens. [Genome view] ENST00000375203; ENSP00000364349; ENSG00000213676; Homo sapiens. [Genome view] ENST00000425571; ENSP00000404814; ENSG00000228628; Homo sapiens. [Genome view] ENST00000427136; ENSP00000404725; ENSG00000234539; Homo sapiens. [Genome view]
GeneID	1388.
KEGG	hsa:1388.
NMPDR	fig|9606.3.peg.26896.
UCSC	uc003nzn.1. human. uc003nzo.1. human.
Organism-specific databases
CTD	1388.
GeneCards	GC06M032194.
H-InvDB	HIX0019631. HIX0057941.
HGNC	HGNC:2349. ATF6B.
MIM	600984. gene.
PharmGKB	PA26867.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q99941.
InParanoid	Q99941.
OMA	RPERKSI.
OrthoDB	EOG9HQH3H.
Gene expression databases
Genevestigator	Q99941.
GermOnline	ENSG00000168477. Homo sapiens.
Family and domain databases
InterPro	IPR011616. bZIP_1. IPR004827. TF_bZIP. [Graphical view]
Pfam	PF00170. bZIP_1. 1 hit. [Graphical view]
SMART	SM00338. BRLZ. 1 hit. [Graphical view]
PROSITE	PS50217. BZIP. 1 hit. PS00036. BZIP_BASIC. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	5641.
PMAP-CutDB	Q99941.
SOURCE	Search...

Entry information

Entry name

ATF6B_HUMAN

Accession

Primary (citable) accession number: Q99941
Secondary accession number(s): Q13269 , Q14343, Q14345, Q5SSW7, Q99635, Q99637, Q9H3V9, Q9H3W1, Q9NPL0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	January 23, 2002
Last modified:	December 15, 2009
This is version 105 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.