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Protein

Opiorphin prepropeptide

Gene

OPRPN

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Opiorphin is an endogenous inhibitor of neprilysin and aminopeptidase N. Inhibits the breakdown of substance P, Mca-BK2 and Met-enkephalin by neprilysin in vitro with IC50 values of 29 µM, 33 µM and 33 µM respectively. Inhibits the breakdown of Ala-pNA by aminopeptidase N in vitro with an IC50 of 65 µM. Has a potent analgesic effect when administered to rats by intravenous injection.1 Publication

GO - Molecular functioni

  • endopeptidase inhibitor activity Source: UniProtKB
  • peptidase inhibitor activity Source: UniProtKB

GO - Biological processi

  • regulation of sensory perception of pain Source: UniProtKB
  • retina homeostasis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor

Names & Taxonomyi

Protein namesi
Recommended name:
Opiorphin prepropeptideImported
Alternative name(s):
Basic proline-rich lacrimal protein
Proline-rich protein 1
Short name:
PRL1
Cleaved into the following chain:
Gene namesi
Name:OPRPNImported
Synonyms:BPLP, PROL1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:17279. OPRPN.

Subcellular locationi

GO - Cellular componenti

  • extracellular region Source: UniProtKB
  • extracellular space Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33803.

Polymorphism and mutation databases

BioMutaiPROL1.
DMDMi290457653.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 21212 PublicationsAdd
BLAST
Chaini22 – 248227Opiorphin prepropeptidePRO_0000022111Add
BLAST
Peptidei22 – 265OpiorphinPRO_0000271169

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei22 – 221Pyrrolidone carboxylic acid2 Publications
Glycosylationi218 – 2181N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein, Pyrrolidone carboxylic acid

Proteomic databases

PaxDbiQ99935.
PeptideAtlasiQ99935.
PRIDEiQ99935.

Expressioni

Tissue specificityi

Abundantly expressed in lacrimal gland where it found in the secretory endpieces. Also expressed at modest levels in the submandibular gland.

Gene expression databases

BgeeiENSG00000171199.
CleanExiHS_PROL1.
ExpressionAtlasiQ99935. baseline and differential.
GenevisibleiQ99935. HS.

Organism-specific databases

HPAiHPA065711.

Interactioni

Protein-protein interaction databases

BioGridi121831. 1 interaction.
STRINGi9606.ENSP00000382485.

Structurei

3D structure databases

ProteinModelPortaliQ99935.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi28 – 142115Pro-richAdd
BLAST
Compositional biasi147 – 22983Thr-richAdd
BLAST

Sequence similaritiesi

Belongs to the PROL1/PROL3 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410KE48. Eukaryota.
ENOG4111CX9. LUCA.
GeneTreeiENSGT00730000111944.
HOGENOMiHOG000015401.
HOVERGENiHBG053686.
InParanoidiQ99935.
OMAiFPLRPYY.
OrthoDBiEOG091G0R4X.
PhylomeDBiQ99935.
TreeFamiTF342130.

Family and domain databases

InterProiIPR026288. SMR-like.
[Graphical view]
PANTHERiPTHR14179. PTHR14179. 2 hits.
PfamiPF15621. PROL5-SMR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99935-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLTFFLGLL ALISCFTPSE SQRFSRRPYL PGQLPPPPLY RPRWVPPSPP
60 70 80 90 100
PPYDSRLNSP LSLPFVPGRV PPSSFSRFSQ AVILSQLFPL ESIRQPRLFP
110 120 130 140 150
GYPNLHFPLR PYYVGPIRIL KPPFPPIPFF LAIYLPISNP EPQINITTAD
160 170 180 190 200
TTITTNPPTT ATATTSTSTK PTMTISSSTV PISSTPEPAT SISAATPAAS
210 220 230 240
TENTTQILAN RPHTVLLNAT VQVTTSNQTI LSSPAFKSFW QKLFAIFG
Length:248
Mass (Da):27,217
Last modified:March 2, 2010 - v2
Checksum:iDB3EF5A6F25C02D5
GO

Sequence cautioni

The sequence AAB46823 differs from that shown. Reason: Frameshift at position 166. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti166 – 1661S → G in AAB46823 (PubMed:8670737).Curated

Mass spectrometryi

Molecular mass is 690 Da from positions 22 - 26. Determined by SELDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S83198 mRNA. Translation: AAB46823.1. Frameshift.
AC106884 Genomic DNA. No translation available.
CH471057 Genomic DNA. Translation: EAX05620.1.
CCDSiCCDS43235.1.
RefSeqiNP_067048.4. NM_021225.4.
UniGeneiHs.661425.

Genome annotation databases

EnsembliENST00000399575; ENSP00000382485; ENSG00000171199.
GeneIDi58503.
KEGGihsa:58503.
UCSCiuc003hfi.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S83198 mRNA. Translation: AAB46823.1. Frameshift.
AC106884 Genomic DNA. No translation available.
CH471057 Genomic DNA. Translation: EAX05620.1.
CCDSiCCDS43235.1.
RefSeqiNP_067048.4. NM_021225.4.
UniGeneiHs.661425.

3D structure databases

ProteinModelPortaliQ99935.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121831. 1 interaction.
STRINGi9606.ENSP00000382485.

Polymorphism and mutation databases

BioMutaiPROL1.
DMDMi290457653.

Proteomic databases

PaxDbiQ99935.
PeptideAtlasiQ99935.
PRIDEiQ99935.

Protocols and materials databases

DNASUi58503.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000399575; ENSP00000382485; ENSG00000171199.
GeneIDi58503.
KEGGihsa:58503.
UCSCiuc003hfi.4. human.

Organism-specific databases

CTDi58503.
GeneCardsiPROL1.
HGNCiHGNC:17279. OPRPN.
HPAiHPA065711.
MIMi608936. gene.
neXtProtiNX_Q99935.
PharmGKBiPA33803.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410KE48. Eukaryota.
ENOG4111CX9. LUCA.
GeneTreeiENSGT00730000111944.
HOGENOMiHOG000015401.
HOVERGENiHBG053686.
InParanoidiQ99935.
OMAiFPLRPYY.
OrthoDBiEOG091G0R4X.
PhylomeDBiQ99935.
TreeFamiTF342130.

Miscellaneous databases

ChiTaRSiPROL1. human.
GenomeRNAii58503.
PROiQ99935.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000171199.
CleanExiHS_PROL1.
ExpressionAtlasiQ99935. baseline and differential.
GenevisibleiQ99935. HS.

Family and domain databases

InterProiIPR026288. SMR-like.
[Graphical view]
PANTHERiPTHR14179. PTHR14179. 2 hits.
PfamiPF15621. PROL5-SMR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPROL1_HUMAN
AccessioniPrimary (citable) accession number: Q99935
Secondary accession number(s): A8MZ07, P85047
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: March 2, 2010
Last modified: September 7, 2016
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.