Reviewed, UniProtKB/Swiss-Prot Q99928 (GBRG3_HUMAN)

Names and origin

Protein names

Recommended name:
    Gamma-aminobutyric acid receptor subunit gamma-3
Alternative name(s):
    GABA(A) receptor subunit gamma-3

Gene names

Name:

GABRG3

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	467 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
Subunit structure	Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho.
Subcellular location	Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
Post-translational modification	May be palmitoylated By similarity.
Miscellaneous	This subunit carries the benzodiazepine binding site.
Sequence similarities	Belongs to the ligand-gated ionic channel (TC 1.A.9) family. [View classification]

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Coding sequence diversity	Polymorphism
Domain	Signal Transmembrane
Ligand	Chloride
Molecular function	Chloride channel Ionic channel
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate
Gene Ontology (GO)
Biological process	chloride transport Inferred from electronic annotation. Source: InterPro gamma-aminobutyric acid signaling pathway Inferred from electronic annotation. Source: InterPro synaptic transmission Non-traceable author statement. Source: UniProtKB
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Non-traceable author statement. Source: UniProtKB postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GABA-A receptor activity Inferred from electronic annotation. Source: InterPro chloride channel activity Inferred from electronic annotation. Source: UniProtKB-KW chloride ion binding Inferred from electronic annotation. Source: UniProtKB-KW extracellular ligand-gated ion channel activity Non-traceable author statement. Source: UniProtKB neurotransmitter receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 467	450	Gamma-aminobutyric acid receptor subunit gamma-3		PRO_0000000481
Regions
	Topological domain	18 – 254	237	Extracellular Probable
	Transmembrane	255 – 277	23	Probable
	Transmembrane	281 – 303	23	Probable
	Transmembrane	315 – 337	23	Probable
	Topological domain	338 – 443	106	Cytoplasmic Probable
	Transmembrane	444 – 467	24	Probable
Amino acid modifications
	Glycosylation	110	1	N-linked (GlcNAc...) Potential
	Glycosylation	228	1	N-linked (GlcNAc...) Potential
	Disulfide bond	171 ↔ 185		By similarity
Natural variations
	Natural variant	352	1	T → A: dbSNP rs2066712.		VAR_033957
Experimental info
	Sequence conflict	386	1	T → P in AAB39369. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q99928-1 [UniParc]. Last modified May 4, 2001. Version 2. Checksum: 1B22D145166438D6	FASTA	467	54,289
	10 20 30 40 50 60 MAPKLLLLLC LFSGLHARSR KVEEDEYEDS SSNQKWVLAP KSQDTDVTLI LNKLLREYDK 70 80 90 100 110 120 KLRPDIGIKP TVIDVDIYVN SIGPVSSINM EYQIDIFFAQ TWTDSRLRFN STMKILTLNS 130 140 150 160 170 180 NMVGLIWIPD TIFRNSKTAE AHWITTPNQL LRIWNDGKIL YTLRLTINAE CQLQLHNFPM 190 200 210 220 230 240 DEHSCPLIFS SYGYPKEEMI YRWRKNSVEA ADQKSWRLYQ FDFMGLRNTT EIVTTSAGDY 250 260 270 280 290 300 VVMTIYFELS RRMGYFTIQT YIPCILTVVL SWVSFWIKKD ATPARTALGI TTVLTMTTLS 310 320 330 340 350 360 TIARKSLPRV SYVTAMDLFV TVCFLFVFAA LMEYATLNYY SSCRKPTTTK KTTSLLHPDS 370 380 390 400 410 420 SRWIPERISL QAPSNYSLLD MRPPPTAMIT LNNSVYWQEF EDTCVYECLD GKDCQSFFCC 430 440 450 460 YEECKSGSWR KGRIHIDILE LDSYSRVFFP TSFLLFNLVY WVGYLYL

References

[1]	"Expression and pharmacology of human GABAA receptors containing gamma 3 subunits." Hadingham K.L., Wafford K.A., Thompson S.A., Palmer K.J., Whiting P.J. Eur. J. Pharmacol. 291:301-309(1995) [PubMed: 8719414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Fetal brain.
[2]	"Gene structure and organization of the GABAA receptor gamma 3 subunit (GABRG3) gene." Han M.K., Chen Y.-H., Nurmi E.L., Sutcliffe J.S. Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Genomic organization of GABAA receptor gamma 3 subunit gene (GABRG3)." Kim S.-J., Gonen D., Yang Z.-Y., Reliford A., Dy A., Leventhal B.L., Cook E.H. Jr. Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 19-467.
+	Additional computationally mapped references.

Web resources

Protein Spotlight

Forbidden fruit - Issue 56 of March 2005

Cross-references

Sequence databases
EMBLGenBankDDBJ	S82769 mRNA. Translation: AAB39369.1. AF269144 , AF269135, AF269136, AF269137, AF269138, AF269139, AF269140, AF269141, AF269142, AF269143 Genomic DNA. Translation: AAF99698.1. AF228458 , AF228450, AF228451, AF228452, AF228453, AF228454, AF228455, AF228456, AF228457 Genomic DNA. Translation: AAF63215.1.
IPI	IPI00294459.
RefSeq	NP_150092.2.
UniGene	Hs.569475 Hs.592027
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q99928.
Proteomic databases
PRIDE	Q99928.
Genome annotation databases
Ensembl	ENSG00000182256. Homo sapiens. [Contig view]
GeneID	2567.
KEGG	hsa:2567.
Organism-specific databases
GeneCards	GC15P024800.
HGNC	HGNC:4088. GABRG3.
MIM	600233. gene.
PharmGKB	PA28502.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q99928.
HOVERGEN	Q99928.
OMA	Q99928. LNNSMYW.
Gene expression databases
ArrayExpress	Q99928.
Bgee	Q99928.
CleanEx	HS_GABRG3.
Family and domain databases
InterPro	IPR006028. GABAA_rcpt. IPR005440. GABBAg3_rcpt. IPR005437. GABBAg_rcpt. IPR006029. Neu_channel_TM. IPR006202. Neur_chan_lig_bd. IPR006201. Neur_channel. IPR018000. Neurotransmitter_ion_chnl_CS. [Graphical view]
Gene3D	G3DSA:2.70.170.10. Neur_chan_lig_bd. 1 hit.
PANTHER	PTHR18945. Neur_channel. 1 hit.
Pfam	PF02931. Neur_chan_LBD. 1 hit. PF02932. Neur_chan_memb. 1 hit. [Graphical view]
PRINTS	PR00253. GABAARECEPTR. PR01620. GABAARGAMMA. PR01623. GABAARGAMMA3. PR00252. NRIONCHANNEL.
TIGRFAMs	TIGR00860. LIC. 1 hit.
PROSITE	PS00236. NEUROTR_ION_CHANNEL. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	10153.
SOURCE	Search...

Entry information

Entry name

GBRG3_HUMAN

Accession

Primary (citable) accession number: Q99928
Secondary accession number(s): Q9HD46, Q9NYT2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	May 4, 2001
Last modified:	June 16, 2009
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)