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Protein

Chymotrypsin-C

Gene

CTRC

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulates activation and degradation of trypsinogens and procarboxypeptidases by targeting specific cleavage sites within their zymogen precursors. Has chymotrypsin-type protease activity and hypocalcemic activity.1 Publication

Catalytic activityi

Preferential cleavage: Leu-|-Xaa, Tyr-|-Xaa, Phe-|-Xaa, Met-|-Xaa, Trp-|-Xaa, Gln-|-Xaa, Asn-|-Xaa.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei74Charge relay systemBy similarity1
Active sitei121Charge relay systemBy similarity1
Active sitei216Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cobalamin metabolic process Source: Reactome
  • proteolysis Source: ProtInc
Complete GO annotation...

Keywordsi

Molecular functionHydrolase, Protease, Serine protease

Enzyme and pathway databases

BioCyciZFISH:HS08679-MONOMER.
BRENDAi3.4.21.2. 2681.
ReactomeiR-HSA-196741. Cobalamin (Cbl, vitamin B12) transport and metabolism.

Protein family/group databases

MEROPSiS01.157.

Names & Taxonomyi

Protein namesi
Recommended name:
Chymotrypsin-C (EC:3.4.21.2)
Alternative name(s):
Caldecrin
Gene namesi
Name:CTRC
Synonyms:CLCR
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:2523. CTRC.

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Involvement in diseasei

Pancreatitis, hereditary (PCTT)4 Publications
Disease susceptibility is associated with variations affecting the gene represented in this entry. Loss-of-function CTRC variants predispose to pancreatitis by diminishing its protective trypsin-degrading activity (PubMed:18059268). They cause loss of function by one or more of three mechanisms: reduced secretion, catalytic defect and increased degradation by trypsin (PubMed:22942235).2 Publications
Disease descriptionA disease characterized by pancreas inflammation, permanent destruction of the pancreatic parenchyma, maldigestion, and severe abdominal pain attacks.
See also OMIM:167800
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_07052232G → V in PCTT; associated with disease susceptibility; highly reduced catalytic efficiency. 1 Publication1
Natural variantiVAR_04352073A → T in PCTT; associated with susceptibility to disease; results in markedly reduced protein secretion. Corresponds to variant dbSNP:rs5157262094 PublicationsEnsembl.1
Natural variantiVAR_070526155C → Y in PCTT; associated with susceptibility to disease; the mutant is not secreted. 2 Publications1
Natural variantiVAR_070528178Q → R in PCTT; associated with disease susceptibility; impaired catalytic activity. Corresponds to variant dbSNP:rs2006781111 PublicationEnsembl.1
Natural variantiVAR_043522217G → R in PCTT; associated with susceptibility to disease; results in markedly reduced protein secretion and loss of activity. 3 Publications1
Natural variantiVAR_043523217G → S in PCTT; associated with susceptibility to disease; reduced protein secretion; impaired catalytic activity. Corresponds to variant dbSNP:rs2020581233 PublicationsEnsembl.1
Natural variantiVAR_043527235V → I in PCTT; associated with susceptibility to disease; slightly reduced activity. Corresponds to variant dbSNP:rs1409932904 PublicationsEnsembl.1
Natural variantiVAR_070536247 – 254Missing in PCTT; associated with disease susceptibility; results in reduced protein secretion and loss of activity. 2 Publications8
Natural variantiVAR_043528249P → L in PCTT; associated with disease susceptibility; results in reduced protein secretion and loss of activity. Corresponds to variant dbSNP:rs1425603292 PublicationsEnsembl.1
Natural variantiVAR_070538250V → E in PCTT; associated with disease susceptibility; results in altered enzyme specificity and loss of activity. 1 Publication1
Natural variantiVAR_043529254R → W in PCTT; associated with susceptibility to disease; results in reduced secretion; normal activity; the mutant undergoes proteolytic degradation during trypsin-mediated activation. Corresponds to variant dbSNP:rs1219092935 PublicationsEnsembl.1

Keywords - Diseasei

Disease mutation

Organism-specific databases

DisGeNETi11330.
MalaCardsiCTRC.
MIMi167800. phenotype.
OpenTargetsiENSG00000162438.
Orphaneti676. Hereditary chronic pancreatitis.
103918. Tropical pancreatitis.
PharmGKBiPA27024.

Chemistry databases

ChEMBLiCHEMBL2386.
GuidetoPHARMACOLOGYi2341.

Polymorphism and mutation databases

BioMutaiCTRC.
DMDMi14194504.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16Sequence analysisAdd BLAST16
PropeptideiPRO_000002771317 – 29Activation peptideAdd BLAST13
ChainiPRO_000002771430 – 268Chymotrypsin-CAdd BLAST239

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi17 ↔ 141PROSITE-ProRule annotation1 Publication
Glycosylationi25N-linked (GlcNAc...)Sequence analysis1
Glycosylationi52N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi59 ↔ 75PROSITE-ProRule annotation1 Publication
Disulfide bondi155 ↔ 222PROSITE-ProRule annotation1 Publication
Disulfide bondi186 ↔ 202PROSITE-ProRule annotation1 Publication
Disulfide bondi212 ↔ 243PROSITE-ProRule annotation1 Publication
Glycosylationi226N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ99895.
PeptideAtlasiQ99895.
PRIDEiQ99895.

Expressioni

Tissue specificityi

Pancreas.

Gene expression databases

BgeeiENSG00000162438.
CleanExiHS_CTRC.
ExpressionAtlasiQ99895. baseline and differential.
GenevisibleiQ99895. HS.

Organism-specific databases

HPAiHPA046920.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
KRTAP10-7P604093EBI-10295404,EBI-10172290

Protein-protein interaction databases

BioGridi116458. 6 interactors.
IntActiQ99895. 5 interactors.
STRINGi9606.ENSP00000365116.

Chemistry databases

BindingDBiQ99895.

Structurei

Secondary structure

1268
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi44 – 51Combined sources8
Beta strandi54 – 65Combined sources12
Beta strandi68 – 71Combined sources4
Helixi73 – 75Combined sources3
Beta strandi82 – 87Combined sources6
Beta strandi100 – 102Combined sources3
Beta strandi104 – 109Combined sources6
Turni115 – 117Combined sources3
Beta strandi123 – 129Combined sources7
Beta strandi155 – 160Combined sources6
Beta strandi162 – 164Combined sources3
Beta strandi174 – 177Combined sources4
Helixi183 – 186Combined sources4
Turni189 – 192Combined sources4
Helixi193 – 195Combined sources3
Beta strandi200 – 203Combined sources4
Beta strandi206 – 210Combined sources5
Beta strandi219 – 223Combined sources5
Beta strandi229 – 239Combined sources11
Beta strandi242 – 245Combined sources4
Beta strandi250 – 254Combined sources5
Helixi255 – 257Combined sources3
Helixi259 – 265Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4H4FX-ray1.90A30-268[»]
Q17-26[»]
ProteinModelPortaliQ99895.
SMRiQ99895.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 267Peptidase S1PROSITE-ProRule annotationAdd BLAST238

Sequence similaritiesi

Belongs to the peptidase S1 family. Elastase subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000119027.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiQ99895.
KOiK01311.
OMAiVDTIHVH.
OrthoDBiEOG091G0DF7.
PhylomeDBiQ99895.
TreeFamiTF330455.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99895-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLGITVLAAL LACASSCGVP SFPPNLSARV VGGEDARPHS WPWQISLQYL
60 70 80 90 100
KNDTWRHTCG GTLIASNFVL TAAHCISNTR TYRVAVGKNN LEVEDEEGSL
110 120 130 140 150
FVGVDTIHVH KRWNALLLRN DIALIKLAEH VELSDTIQVA CLPEKDSLLP
160 170 180 190 200
KDYPCYVTGW GRLWTNGPIA DKLQQGLQPV VDHATCSRID WWGFRVKKTM
210 220 230 240 250
VCAGGDGVIS ACNGDSGGPL NCQLENGSWE VFGIVSFGSR RGCNTRKKPV
260
VYTRVSAYID WINEKMQL
Length:268
Mass (Da):29,484
Last modified:June 1, 2001 - v2
Checksum:i460BF33B4A96516F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti16S → T in AAB47104 (PubMed:8635596).Curated1
Sequence conflicti52N → D in CAA74031 (Ref. 6) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_07052018G → R Found in a patient with chronic pancreatitis; unknown pathological significance; catalytic activity comparable to that of wild type; the mutant undergoes proteolytic degradation during trypsin-mediated activation. Corresponds to variant dbSNP:rs2005769651 PublicationEnsembl.1
Natural variantiVAR_07052129R → Q Found in patients with chronic pancreatitis; unknown pathological significance. Corresponds to variant dbSNP:rs7720249861 PublicationEnsembl.1
Natural variantiVAR_07052232G → V in PCTT; associated with disease susceptibility; highly reduced catalytic efficiency. 1 Publication1
Natural variantiVAR_04351635D → H. 1 Publication1
Natural variantiVAR_04351735D → N. Corresponds to variant dbSNP:rs1849774211 PublicationEnsembl.1
Natural variantiVAR_07052335D → Y Found in a patient with chronic pancreatitis; unknown pathological significance; catalytic activity comparable to that of wild type. 1 Publication1
Natural variantiVAR_04351837R → Q Rare variant; results in normal secretion and activity. Corresponds to variant dbSNP:rs1458682781 PublicationEnsembl.1
Natural variantiVAR_04351948Q → R Rare variant that may be associated with susceptibility to pancreatitis; results in markedly reduced protein secretion. Corresponds to variant dbSNP:rs5368129163 PublicationsEnsembl.1
Natural variantiVAR_07052461G → R Found in a patient with chronic pancreatitis; unknown pathological significance; the mutant is not secreted. Corresponds to variant dbSNP:rs7694820361 PublicationEnsembl.1
Natural variantiVAR_04352073A → T in PCTT; associated with susceptibility to disease; results in markedly reduced protein secretion. Corresponds to variant dbSNP:rs5157262094 PublicationsEnsembl.1
Natural variantiVAR_01092880R → W. Corresponds to variant dbSNP:rs7796437101 PublicationEnsembl.1
Natural variantiVAR_070525151K → N Found in a patient with chronic pancreatitis; unknown pathological significance. 1
Natural variantiVAR_070526155C → Y in PCTT; associated with susceptibility to disease; the mutant is not secreted. 2 Publications1
Natural variantiVAR_070527162R → H Rare variant; found in a patient with chronic pancreatitis; unknown pathological significance. Corresponds to variant dbSNP:rs7754044791 PublicationEnsembl.1
Natural variantiVAR_043521172K → E. Corresponds to variant dbSNP:rs349496353 PublicationsEnsembl.1
Natural variantiVAR_070528178Q → R in PCTT; associated with disease susceptibility; impaired catalytic activity. Corresponds to variant dbSNP:rs2006781111 PublicationEnsembl.1
Natural variantiVAR_070529200M → V Rare variant; found in a patient with chronic pancreatitis; unknown pathological significance. Corresponds to variant dbSNP:rs1462354991 PublicationEnsembl.1
Natural variantiVAR_070530209I → M. 1 Publication1
Natural variantiVAR_043522217G → R in PCTT; associated with susceptibility to disease; results in markedly reduced protein secretion and loss of activity. 3 Publications1
Natural variantiVAR_043523217G → S in PCTT; associated with susceptibility to disease; reduced protein secretion; impaired catalytic activity. Corresponds to variant dbSNP:rs2020581233 PublicationsEnsembl.1
Natural variantiVAR_043524218G → S. 1 Publication1
Natural variantiVAR_043525220L → R Rare variant; results in impaired protein secretion. 2 Publications1
Natural variantiVAR_043526225E → A. Corresponds to variant dbSNP:rs2014866131 PublicationEnsembl.1
Natural variantiVAR_070531225E → K Found in a patient with chronic pancreatitis; unknown pathological significance. 1 Publication1
Natural variantiVAR_070532227G → S. Corresponds to variant dbSNP:rs5677452131 PublicationEnsembl.1
Natural variantiVAR_043527235V → I in PCTT; associated with susceptibility to disease; slightly reduced activity. Corresponds to variant dbSNP:rs1409932904 PublicationsEnsembl.1
Natural variantiVAR_070533239S → A Found in patients with chronic pancreatitis; unknown pathological significance. 1 Publication1
Natural variantiVAR_070534239S → C Found in patients with chronic pancreatitis; unknown pathological significance. 1 Publication1
Natural variantiVAR_070535246R → C. Corresponds to variant dbSNP:rs2004123141 PublicationEnsembl.1
Natural variantiVAR_070536247 – 254Missing in PCTT; associated with disease susceptibility; results in reduced protein secretion and loss of activity. 2 Publications8
Natural variantiVAR_070537247K → E Found in patients with chronic pancreatitis; unknown pathological significance. 1 Publication1
Natural variantiVAR_043528249P → L in PCTT; associated with disease susceptibility; results in reduced protein secretion and loss of activity. Corresponds to variant dbSNP:rs1425603292 PublicationsEnsembl.1
Natural variantiVAR_070538250V → E in PCTT; associated with disease susceptibility; results in altered enzyme specificity and loss of activity. 1 Publication1
Natural variantiVAR_070539254R → Q Found in a patient with chronic pancreatitis; unknown pathological significance; mutant protein secretion, activity and trypsin-mediated degradation are comparable to those of wild-type. Corresponds to variant dbSNP:rs7558118992 PublicationsEnsembl.1
Natural variantiVAR_043529254R → W in PCTT; associated with susceptibility to disease; results in reduced secretion; normal activity; the mutant undergoes proteolytic degradation during trypsin-mediated activation. Corresponds to variant dbSNP:rs1219092935 PublicationsEnsembl.1
Natural variantiVAR_070540257A → T Found in patients with chronic pancreatitis; unknown pathological significance. Corresponds to variant dbSNP:rs2004066961 PublicationEnsembl.1
Natural variantiVAR_043530260D → N. Corresponds to variant dbSNP:rs5407538751 PublicationEnsembl.1
Natural variantiVAR_070541263N → S Found in a patient with chronic pancreatitis; unknown pathological significance. Corresponds to variant dbSNP:rs7699751641 PublicationEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S82198 mRNA. Translation: AAB47104.2. Sequence problems.
AK289447 mRNA. Translation: BAF82136.1.
AL031283 Genomic DNA. Translation: CAB77355.1.
CH471167 Genomic DNA. Translation: EAW51726.1.
BC015118 mRNA. Translation: AAH15118.1.
Y13697 mRNA. Translation: CAA74031.1.
CCDSiCCDS156.1.
PIRiS68825.
S68826.
RefSeqiNP_009203.2. NM_007272.2.
UniGeneiHs.631869.

Genome annotation databases

EnsembliENST00000375949; ENSP00000365116; ENSG00000162438.
GeneIDi11330.
KEGGihsa:11330.
UCSCiuc001awi.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S82198 mRNA. Translation: AAB47104.2. Sequence problems.
AK289447 mRNA. Translation: BAF82136.1.
AL031283 Genomic DNA. Translation: CAB77355.1.
CH471167 Genomic DNA. Translation: EAW51726.1.
BC015118 mRNA. Translation: AAH15118.1.
Y13697 mRNA. Translation: CAA74031.1.
CCDSiCCDS156.1.
PIRiS68825.
S68826.
RefSeqiNP_009203.2. NM_007272.2.
UniGeneiHs.631869.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4H4FX-ray1.90A30-268[»]
Q17-26[»]
ProteinModelPortaliQ99895.
SMRiQ99895.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116458. 6 interactors.
IntActiQ99895. 5 interactors.
STRINGi9606.ENSP00000365116.

Chemistry databases

BindingDBiQ99895.
ChEMBLiCHEMBL2386.
GuidetoPHARMACOLOGYi2341.

Protein family/group databases

MEROPSiS01.157.

Polymorphism and mutation databases

BioMutaiCTRC.
DMDMi14194504.

Proteomic databases

PaxDbiQ99895.
PeptideAtlasiQ99895.
PRIDEiQ99895.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000375949; ENSP00000365116; ENSG00000162438.
GeneIDi11330.
KEGGihsa:11330.
UCSCiuc001awi.2. human.

Organism-specific databases

CTDi11330.
DisGeNETi11330.
GeneCardsiCTRC.
HGNCiHGNC:2523. CTRC.
HPAiHPA046920.
MalaCardsiCTRC.
MIMi167800. phenotype.
601405. gene.
neXtProtiNX_Q99895.
OpenTargetsiENSG00000162438.
Orphaneti676. Hereditary chronic pancreatitis.
103918. Tropical pancreatitis.
PharmGKBiPA27024.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000119027.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiQ99895.
KOiK01311.
OMAiVDTIHVH.
OrthoDBiEOG091G0DF7.
PhylomeDBiQ99895.
TreeFamiTF330455.

Enzyme and pathway databases

BioCyciZFISH:HS08679-MONOMER.
BRENDAi3.4.21.2. 2681.
ReactomeiR-HSA-196741. Cobalamin (Cbl, vitamin B12) transport and metabolism.

Miscellaneous databases

ChiTaRSiCTRC. human.
GeneWikiiChymotrypsin-C.
GenomeRNAii11330.
PROiQ99895.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000162438.
CleanExiHS_CTRC.
ExpressionAtlasiQ99895. baseline and differential.
GenevisibleiQ99895. HS.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCTRC_HUMAN
AccessioniPrimary (citable) accession number: Q99895
Secondary accession number(s): A8K082, O00765, Q9NUH5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: June 1, 2001
Last modified: January 18, 2017
This is version 143 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Peptidase families
    Classification of peptidase families and list of entries
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.