Q99856 (ARI3A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
AT-rich interactive domain-containing protein 3A
Short name=ARID domain-containing protein 3A

Alternative name(s):
B-cell regulator of IgH transcription
Short name=Bright
Dead ringer-like protein 1
E2F-binding protein 1

Gene names

Name:	ARID3A
Synonyms:	DRIL1, DRIL3, DRX, E2FBP1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	593 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription factor which may be involved in the control of cell cycle progression by the RB1/E2F1 pathway and in B-cell differentiation. Ref.6 Ref.7
Subunit structure	Homodimer. Heterodimer with ARID3B. Interacts with E2F1. Interacts with GTF2I and BTK. Ref.2 Ref.10 Ref.11
Subcellular location	Nucleus. Cytoplasm. Note: Shuttles between nucleus and cytoplasm. Ref.11
Tissue specificity	Widely expressed, with highest expression in skeletal muscle, thalamus, and colon.
Induction	By p53/TP53 following DNA damage. Ref.7
Sequence similarities	Contains 1 ARID domain. Contains 1 REKLES domain.
Sequence caution	The sequence AAW30734.1 differs from that shown. Reason: Frameshift at position 498.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Polymorphism
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Non-traceable author statement. Source: UniProtKB
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 593

593

AT-rich interactive domain-containing protein 3A

PRO_0000200578

Regions

Domain

238 – 330

93

ARID

Domain

444 – 541

98

REKLES

Region

119 – 156

38

Acidic

Region

445 – 488

44

Important for nuclear localization By similarity

Region

490 – 513

24

Homodimerization

Region

537 – 557

21

Important for cytoplasmic localization By similarity

Compositional bias

67 – 70

4

Poly-Ala

Compositional bias

89 – 157

69

Glu-rich

Compositional bias

424 – 445

22

Ala-rich

Compositional bias

550 – 579

30

Gly-rich

Amino acid modifications

Modified residue

77

1

Phosphoserine Ref.9 Ref.12 Ref.13 Ref.14

Modified residue

81

1

Phosphoserine Ref.8 Ref.9 Ref.12 Ref.13 Ref.14

Modified residue

88

1

Phosphoserine Ref.8 Ref.9 Ref.12 Ref.13 Ref.14

Modified residue

119

1

Phosphoserine By similarity

Natural variations

Natural variant

36

1

P → H. Ref.5
Corresponds to variant rs17857499 [ dbSNP | Ensembl ].

VAR_033203

Natural variant

320

1

K → E. Ref.5
Corresponds to variant rs17857501 [ dbSNP | Ensembl ].

VAR_033204

Natural variant

556

1

G → S. Ref.2 Ref.5
Corresponds to variant rs1051505 [ dbSNP | Ensembl ].

VAR_033205

Experimental info

Mutagenesis

325

1

Y → A: Abolishes DNA-binding. Ref.10

Mutagenesis

461

1

K → A: Abolishes nuclear targeting. Ref.11

Mutagenesis

527

1

G → A or P: Impairs DNA-binding but not self-association. Ref.11

Mutagenesis

530

1

Y → A: Impairs DNA-binding but not self-association. Ref.11

Mutagenesis

530

1

Y → F: No effect on DNA-binding. Ref.11

Mutagenesis

532

1

G → A: Impairs DNA-binding. Ref.11

Mutagenesis

534

1

L → A: Impairs DNA-binding. Ref.11

Sequence conflict

423

1

V → S in AAN74028. Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

593

Helix Strand Turn

Details...

Helix

231 – 234

4

Helix

239 – 254

16

Helix

272 – 281

10

Helix

286 – 291

6

Helix

294 – 298

5

Helix

310 – 321

12

Helix

324 – 329

6

Helix

335 – 346

12

Sequences

	Sequence		Length	Mass (Da)
	Q99856 [UniParc]. Last modified January 1, 1998. Version 2. Checksum: 4D09131E168A2880	FASTA	593	62,889
	10 20 30 40 50 60 MKLQAVMETL LQRQQRARQE LEARQQLPPD PPAAPPGRAR AAPDEDREPE SARMQRAQMA 70 80 90 100 110 120 ALAAMRAAAA GLGHPASPGG SEDGPPGSEE EDAAREGTPG SPGRGREGPG EEHFEDMASD 130 140 150 160 170 180 EDMKPKWEEE EMEEDLGEDE EEEEEDYEDE EEEEDEEGLG PPGPASLGTT ALFPRKAQPP 190 200 210 220 230 240 QAFRGDGVPR VLGGQERPGP GPAHPGGAAH VAPQLQPPDH GDWTYEEQFK QLYELDGDPK 250 260 270 280 290 300 RKEFLDDLFS FMQKRGTPVN RIPIMAKQVL DLFMLYVLVT EKGGLVEVIN KKLWREITKG 310 320 330 340 350 360 LNLPTSITSA AFTLRTQYMK YLYPYECEKR GLSNPNELQA AIDSNRREGR RQSFGGSLFA 370 380 390 400 410 420 YSPGGAHGML SSPKLPVSSL GLAASTNGSS ITPAPKIKKE EDSAIPITVP GRLPVSLAGH 430 440 450 460 470 480 PVVAAQAAAV QAAAAQAAVA AQAAALEQLR EKLESAEPPE KKMALVADEQ QRLMQRALQQ 490 500 510 520 530 540 NFLAMAAQLP MSIRINSQAS ESRQDSAVNL TGTNGSNSIS MSVEINGIMY TGVLFAQPPA 550 560 570 580 590 PTPTSAPNKG GGGGGGSSSN AGGRGGNTGT SGGQAGPAGL STPSTSTSNN SLP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The human dead ringer/bright homolog, DRIL1: cDNA cloning, gene structure, and mapping to D19S886, a marker on 19p13.3 that is strictly linked to the Peutz-Jeghers syndrome." Kortschak R.D., Reimann H., Zimmer M., Eyre H.J., Saint R., Jenne D.E. Genomics 51:288-292(1998) [PubMed: 9722953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]	"A novel E2F binding protein with Myc-type HLH motif stimulates E2F-dependent transcription by forming a heterodimer." Suzuki M., Okuyama S., Okamoto S., Shirasuna K., Nakajima T., Hachiya T., Nojima H., Sekiya S., Oda K. Oncogene 17:853-865(1998) [PubMed: 9780002] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH E2F1, VARIANT SER-556.
[3]	"Molecular cloning and immunologic characterization of DRIL3, a new bright-like ARID protein expressed in both myeloid and lymphoid cells, shares homology to E2FBP1/pRB family complexes." Paulin Y.G., Frank R.T., Davy E.J. Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Placenta.
[4]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed: 15057824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS HIS-36; GLU-320 AND SER-556. Tissue: Placenta.
[6]	"A functional screen identifies hDRIL1 as an oncogene that rescues RAS-induced senescence." Peeper D.S., Shvarts A., Brummelkamp T., Douma S., Koh E.Y., Daley G.Q., Bernards R. Nat. Cell Biol. 4:148-153(2002) [PubMed: 11812999] [Abstract] Cited for: FUNCTION.
[7]	"E2FBP1/DRIL1, an AT-rich interaction domain-family transcription factor, is regulated by p53." Ma K., Araki K., Ichwan S.J.A., Suganuma T., Tamamori-Adachi M., Ikeda M.-A. Mol. Cancer Res. 1:438-444(2003) [PubMed: 12692263] [Abstract] Cited for: FUNCTION, INDUCTION.
[8]	"Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-81 AND SER-88, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77; SER-81 AND SER-88, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"Induction of immunoglobulin heavy-chain transcription through the transcription factor Bright requires TFII-I." Rajaiya J., Nixon J.C., Ayers N., Desgranges Z.P., Roy A.L., Webb C.F. Mol. Cell. Biol. 26:4758-4768(2006) [PubMed: 16738337] [Abstract] Cited for: INTERACTION WITH GTF2I AND BTK, SUBUNIT, DNA-BINDING, MUTAGENESIS OF TYR-325.
[11]	"REKLES is an ARID3-restricted multifunctional domain." Kim D., Probst L., Das C., Tucker P.W. J. Biol. Chem. 282:15768-15777(2007) [PubMed: 17400556] [Abstract] Cited for: INTERACTION WITH ARID3B, DNA-BINDING, SUBUNIT, SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-461; GLY-527; TYR-530; GLY-532 AND LEU-534.
[12]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77; SER-81 AND SER-88, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[13]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77; SER-81 AND SER-88, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[14]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77; SER-81 AND SER-88, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[15]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[16]	"Solution NMR structure of the ARID domain of human AT-rich interactive domain-containing protein 3A: a human cancer protein interaction network target." Liu G., Huang Y.J., Xiao R., Wang D., Acton T.B., Montelione G.T. Proteins 78:2170-2175(2010) [PubMed: 20455271] [Abstract] Cited for: STRUCTURE BY NMR OF 218-351.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U88047 mRNA. Translation: AAC32888.1.
AF039850 , AF039844, AF039845, AF039846, AF039847, AF039848, AF039849 Genomic DNA. Translation: AAC69994.1.
AY152547 mRNA. Translation: AAN74028.1.
AY845638 mRNA. Translation: AAW30734.1. Frameshift.
AC005391 Genomic DNA. Translation: AAC28918.1.
AC005379 Genomic DNA. Translation: AAC28499.1.
BC033163 mRNA. Translation: AAH33163.1.
BC060828 mRNA. Translation: AAH60828.1.

IPI

IPI00018500.

RefSeq

NP_005215.1. NM_005224.2.

UniGene

Hs.501296.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2KK0	NMR	-	A	218-351	[»]

ProteinModelPortal

Q99856.

SMR

Q99856. Positions 218-351.

ModBase

Search...

Protein-protein interaction databases

STRING

Q99856.

PTM databases

PhosphoSite

Q99856.

Polymorphism databases

DMDM

12230034.

Proteomic databases

PRIDE

Q99856.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000263620; ENSP00000263620; ENSG00000116017.

GeneID

1820.

KEGG

hsa:1820.

UCSC

uc002lql.1. human.

Organism-specific databases

CTD

1820.

GeneCards

GC19P000926.

H-InvDB

HIX0040051.

HGNC

HGNC:3031. ARID3A.

HPA

HPA004793.

MIM

603265. gene.

neXtProt

NX_Q99856.

PharmGKB

PA27485.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG10997.

GeneTree

ENSGT00550000074575.

HOGENOM

HBG402789.

HOVERGEN

HBG050574.

InParanoid

Q99856.

OMA

PPDHGDW.

OrthoDB

EOG4Z36GC.

PhylomeDB

Q99856.

Gene expression databases

ArrayExpress

Q99856.

Bgee

Q99856.

CleanEx

HS_ARID3A.

Genevestigator

Q99856.

GermOnline

ENSG00000116017. Homo sapiens.

Family and domain databases

InterPro

IPR001606. ARID/BRIGHT_DNA-bd.
IPR023334. REKLES_domain.
[Graphical view]

Gene3D

G3DSA:1.10.150.60. ARID. 1 hit.

Pfam

PF01388. ARID. 1 hit.
[Graphical view]

SMART

SM00501. BRIGHT. 1 hit.
[Graphical view]

SUPFAM

SSF46774. ARID. 1 hit.

PROSITE

PS51011. ARID. 1 hit.
PS51486. REKLES. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

7417.

SOURCE

Search...

Entry information

Entry name

ARI3A_HUMAN

Accession

Primary (citable) accession number: Q99856
Secondary accession number(s): Q5I858 , Q6P9C6, Q8IZA7, Q8N4Z3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	January 1, 1998
Last modified:	January 25, 2012
This is version 103 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.