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Protein

AT-rich interactive domain-containing protein 3A

Gene

ARID3A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor which may be involved in the control of cell cycle progression by the RB1/E2F1 pathway and in B-cell differentiation.2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000116017-MONOMER.
ReactomeiR-HSA-6804116. TP53 Regulates Transcription of Genes Involved in G1 Cell Cycle Arrest.

Names & Taxonomyi

Protein namesi
Recommended name:
AT-rich interactive domain-containing protein 3A
Short name:
ARID domain-containing protein 3A
Alternative name(s):
B-cell regulator of IgH transcription
Short name:
Bright
Dead ringer-like protein 1
E2F-binding protein 1
Gene namesi
Name:ARID3A
Synonyms:DRIL1, DRIL3, DRX, E2FBP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:3031. ARID3A.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation1 Publication
  • Cytoplasm 1 Publication

  • Note: Shuttles between nucleus and cytoplasm.

GO - Cellular componenti

  • cytoplasm Source: HPA
  • Golgi apparatus Source: HPA
  • membrane raft Source: MGI
  • nucleolus Source: HPA
  • nucleoplasm Source: HPA
  • nucleus Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi325Y → A: Abolishes DNA-binding. 1 Publication1
Mutagenesisi461K → A: Abolishes nuclear targeting. 1 Publication1
Mutagenesisi527G → A or P: Impairs DNA-binding but not self-association. 1 Publication1
Mutagenesisi530Y → A: Impairs DNA-binding but not self-association. 1 Publication1
Mutagenesisi530Y → F: No effect on DNA-binding. 1 Publication1
Mutagenesisi532G → A: Impairs DNA-binding. 1 Publication1
Mutagenesisi534L → A: Impairs DNA-binding. 1 Publication1

Organism-specific databases

DisGeNETi1820.
OpenTargetsiENSG00000116017.
PharmGKBiPA27485.

Polymorphism and mutation databases

BioMutaiARID3A.
DMDMi12230034.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002005781 – 593AT-rich interactive domain-containing protein 3AAdd BLAST593

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei77PhosphoserineCombined sources1
Modified residuei81PhosphoserineCombined sources1
Modified residuei88PhosphoserineCombined sources1
Modified residuei98PhosphothreonineCombined sources1
Modified residuei101PhosphoserineCombined sources1
Modified residuei119PhosphoserineCombined sources1
Modified residuei353PhosphoserineCombined sources1
Modified residuei362PhosphoserineCombined sources1
Cross-linki452Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki462Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ99856.
MaxQBiQ99856.
PaxDbiQ99856.
PeptideAtlasiQ99856.
PRIDEiQ99856.

PTM databases

iPTMnetiQ99856.
PhosphoSitePlusiQ99856.

Expressioni

Tissue specificityi

Widely expressed, with highest expression in skeletal muscle, thalamus, and colon.

Inductioni

By p53/TP53 following DNA damage.1 Publication

Gene expression databases

BgeeiENSG00000116017.
CleanExiHS_ARID3A.
ExpressionAtlasiQ99856. baseline and differential.
GenevisibleiQ99856. HS.

Organism-specific databases

HPAiHPA004793.

Interactioni

Subunit structurei

Homodimer. Heterodimer with ARID3B. Interacts with E2F1. Interacts with GTF2I and BTK.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BTKQ061873EBI-5458244,EBI-624835
NOTCH2NLQ7Z3S93EBI-5458244,EBI-945833
TTC32Q5I0X73EBI-5458244,EBI-8636434

Protein-protein interaction databases

BioGridi108154. 25 interactors.
IntActiQ99856. 19 interactors.
MINTiMINT-4537882.
STRINGi9606.ENSP00000263620.

Structurei

Secondary structure

1593
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi225 – 228Combined sources4
Helixi230 – 233Combined sources4
Helixi239 – 255Combined sources17
Helixi272 – 281Combined sources10
Helixi285 – 291Combined sources7
Helixi294 – 300Combined sources7
Helixi310 – 321Combined sources12
Helixi323 – 329Combined sources7
Helixi335 – 343Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2KK0NMR-A218-351[»]
4LJXX-ray2.21A/B216-351[»]
ProteinModelPortaliQ99856.
SMRiQ99856.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ99856.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini238 – 330ARIDPROSITE-ProRule annotationAdd BLAST93
Domaini444 – 541REKLESPROSITE-ProRule annotationAdd BLAST98

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni119 – 156AcidicAdd BLAST38
Regioni445 – 488Important for nuclear localizationBy similarityAdd BLAST44
Regioni490 – 513HomodimerizationAdd BLAST24
Regioni537 – 557Important for cytoplasmic localizationBy similarityAdd BLAST21

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi67 – 70Poly-Ala4
Compositional biasi89 – 157Glu-richAdd BLAST69
Compositional biasi424 – 445Ala-richAdd BLAST22
Compositional biasi550 – 579Gly-richAdd BLAST30

Sequence similaritiesi

Contains 1 ARID domain.PROSITE-ProRule annotation
Contains 1 REKLES domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2744. Eukaryota.
ENOG410Y2AP. LUCA.
GeneTreeiENSGT00550000074575.
HOGENOMiHOG000236281.
HOVERGENiHBG050574.
InParanoidiQ99856.
OMAiLFAYSPG.
OrthoDBiEOG091G16XQ.
PhylomeDBiQ99856.
TreeFamiTF320364.

Family and domain databases

Gene3Di1.10.150.60. 1 hit.
InterProiIPR001606. ARID_dom.
IPR023334. REKLES_domain.
[Graphical view]
PfamiPF01388. ARID. 1 hit.
[Graphical view]
SMARTiSM00501. BRIGHT. 1 hit.
[Graphical view]
SUPFAMiSSF46774. SSF46774. 1 hit.
PROSITEiPS51011. ARID. 1 hit.
PS51486. REKLES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99856-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLQAVMETL LQRQQRARQE LEARQQLPPD PPAAPPGRAR AAPDEDREPE
60 70 80 90 100
SARMQRAQMA ALAAMRAAAA GLGHPASPGG SEDGPPGSEE EDAAREGTPG
110 120 130 140 150
SPGRGREGPG EEHFEDMASD EDMKPKWEEE EMEEDLGEDE EEEEEDYEDE
160 170 180 190 200
EEEEDEEGLG PPGPASLGTT ALFPRKAQPP QAFRGDGVPR VLGGQERPGP
210 220 230 240 250
GPAHPGGAAH VAPQLQPPDH GDWTYEEQFK QLYELDGDPK RKEFLDDLFS
260 270 280 290 300
FMQKRGTPVN RIPIMAKQVL DLFMLYVLVT EKGGLVEVIN KKLWREITKG
310 320 330 340 350
LNLPTSITSA AFTLRTQYMK YLYPYECEKR GLSNPNELQA AIDSNRREGR
360 370 380 390 400
RQSFGGSLFA YSPGGAHGML SSPKLPVSSL GLAASTNGSS ITPAPKIKKE
410 420 430 440 450
EDSAIPITVP GRLPVSLAGH PVVAAQAAAV QAAAAQAAVA AQAAALEQLR
460 470 480 490 500
EKLESAEPPE KKMALVADEQ QRLMQRALQQ NFLAMAAQLP MSIRINSQAS
510 520 530 540 550
ESRQDSAVNL TGTNGSNSIS MSVEINGIMY TGVLFAQPPA PTPTSAPNKG
560 570 580 590
GGGGGGSSSN AGGRGGNTGT SGGQAGPAGL STPSTSTSNN SLP
Length:593
Mass (Da):62,889
Last modified:January 1, 1998 - v2
Checksum:i4D09131E168A2880
GO

Sequence cautioni

The sequence AAW30734 differs from that shown. Reason: Frameshift at position 498.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti423V → S in AAN74028 (PubMed:9780002).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03320336P → H.1 PublicationCorresponds to variant rs17857499dbSNPEnsembl.1
Natural variantiVAR_033204320K → E.1 PublicationCorresponds to variant rs17857501dbSNPEnsembl.1
Natural variantiVAR_033205556G → S.2 PublicationsCorresponds to variant rs1051505dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U88047 mRNA. Translation: AAC32888.1.
AF039850
, AF039844, AF039845, AF039846, AF039847, AF039848, AF039849 Genomic DNA. Translation: AAC69994.1.
AY152547 mRNA. Translation: AAN74028.1.
AY845638 mRNA. Translation: AAW30734.1. Frameshift.
AC005391 Genomic DNA. Translation: AAC28918.1.
AC005379 Genomic DNA. Translation: AAC28499.1.
BC033163 mRNA. Translation: AAH33163.1.
BC060828 mRNA. Translation: AAH60828.1.
CCDSiCCDS12050.1.
RefSeqiNP_005215.1. NM_005224.2.
XP_005259570.1. XM_005259513.4.
XP_005259571.1. XM_005259514.4.
XP_016881934.1. XM_017026445.1.
UniGeneiHs.501296.
Hs.660769.

Genome annotation databases

EnsembliENST00000263620; ENSP00000263620; ENSG00000116017.
GeneIDi1820.
KEGGihsa:1820.
UCSCiuc002lql.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U88047 mRNA. Translation: AAC32888.1.
AF039850
, AF039844, AF039845, AF039846, AF039847, AF039848, AF039849 Genomic DNA. Translation: AAC69994.1.
AY152547 mRNA. Translation: AAN74028.1.
AY845638 mRNA. Translation: AAW30734.1. Frameshift.
AC005391 Genomic DNA. Translation: AAC28918.1.
AC005379 Genomic DNA. Translation: AAC28499.1.
BC033163 mRNA. Translation: AAH33163.1.
BC060828 mRNA. Translation: AAH60828.1.
CCDSiCCDS12050.1.
RefSeqiNP_005215.1. NM_005224.2.
XP_005259570.1. XM_005259513.4.
XP_005259571.1. XM_005259514.4.
XP_016881934.1. XM_017026445.1.
UniGeneiHs.501296.
Hs.660769.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2KK0NMR-A218-351[»]
4LJXX-ray2.21A/B216-351[»]
ProteinModelPortaliQ99856.
SMRiQ99856.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108154. 25 interactors.
IntActiQ99856. 19 interactors.
MINTiMINT-4537882.
STRINGi9606.ENSP00000263620.

PTM databases

iPTMnetiQ99856.
PhosphoSitePlusiQ99856.

Polymorphism and mutation databases

BioMutaiARID3A.
DMDMi12230034.

Proteomic databases

EPDiQ99856.
MaxQBiQ99856.
PaxDbiQ99856.
PeptideAtlasiQ99856.
PRIDEiQ99856.

Protocols and materials databases

DNASUi1820.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000263620; ENSP00000263620; ENSG00000116017.
GeneIDi1820.
KEGGihsa:1820.
UCSCiuc002lql.4. human.

Organism-specific databases

CTDi1820.
DisGeNETi1820.
GeneCardsiARID3A.
HGNCiHGNC:3031. ARID3A.
HPAiHPA004793.
MIMi603265. gene.
neXtProtiNX_Q99856.
OpenTargetsiENSG00000116017.
PharmGKBiPA27485.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2744. Eukaryota.
ENOG410Y2AP. LUCA.
GeneTreeiENSGT00550000074575.
HOGENOMiHOG000236281.
HOVERGENiHBG050574.
InParanoidiQ99856.
OMAiLFAYSPG.
OrthoDBiEOG091G16XQ.
PhylomeDBiQ99856.
TreeFamiTF320364.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000116017-MONOMER.
ReactomeiR-HSA-6804116. TP53 Regulates Transcription of Genes Involved in G1 Cell Cycle Arrest.

Miscellaneous databases

EvolutionaryTraceiQ99856.
GeneWikiiARID3A.
GenomeRNAii1820.
PROiQ99856.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000116017.
CleanExiHS_ARID3A.
ExpressionAtlasiQ99856. baseline and differential.
GenevisibleiQ99856. HS.

Family and domain databases

Gene3Di1.10.150.60. 1 hit.
InterProiIPR001606. ARID_dom.
IPR023334. REKLES_domain.
[Graphical view]
PfamiPF01388. ARID. 1 hit.
[Graphical view]
SMARTiSM00501. BRIGHT. 1 hit.
[Graphical view]
SUPFAMiSSF46774. SSF46774. 1 hit.
PROSITEiPS51011. ARID. 1 hit.
PS51486. REKLES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARI3A_HUMAN
AccessioniPrimary (citable) accession number: Q99856
Secondary accession number(s): Q5I858
, Q6P9C6, Q8IZA7, Q8N4Z3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 1, 1998
Last modified: November 30, 2016
This is version 150 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.