Reviewed, UniProtKB/Swiss-Prot Q99856 (ARI3A_HUMAN)

Names and origin

Protein names

Recommended name:
    AT-rich interactive domain-containing protein 3A
Alternative name(s):
    ARID domain-containing protein 3A
    Dead ringer-like protein 1
    B-cell regulator of IgH transcription
      Short name=Bright
    E2F-binding protein 1

Gene names

Name:	ARID3A
Synonyms:	DRIL1, DRIL3, DRX, E2FBP1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	593 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Transcription factor which may be involved in the control of cell cycle progression by the RB1/E2F1 pathway and in B-cell differentiation. Ref.6 Ref.7
Subunit structure	Homodimer. Heterodimer with ARID3B. Interacts with E2F1. Interacts with GTF2I and BTK. Ref.2 Ref.10 Ref.11
Subcellular location	Nucleus. Cytoplasm. Note: Shuttles between nucleus and cytoplasm. Ref.11
Tissue specificity	Widely expressed, with highest expression in skeletal muscle, thalamus, and colon.
Induction	By TP53 following DNA damage. Ref.7
Sequence similarities	Contains 1 ARID domain. Contains 1 NAP (nucleosome assembly protein) domain.
Sequence caution	The sequence AAW30734.1 differs from that shown. Reason: Frameshift at position 498.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Polymorphism
Ligand	DNA-binding
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Non-traceable author statement. Source: UniProtKB transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi apparatus Inferred from direct assay. Source: HPA nucleus Non-traceable author statement. Source: UniProtKB
Molecular function	transcription factor activity Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 593	593	AT-rich interactive domain-containing protein 3A		PRO_0000200578
Regions
	Domain	129 – 157	29	NAP
	Domain	238 – 330	93	ARID
	Region	445 – 488	44	Important for nuclear localization By similarity
	Region	490 – 513	24	Homodimerization
	Region	537 – 557	21	Important for cytoplasmic localization By similarity
	Compositional bias	67 – 70	4	Poly-Ala
	Compositional bias	89 – 157	69	Glu-rich
	Compositional bias	424 – 445	22	Ala-rich
	Compositional bias	550 – 579	30	Gly-rich
Amino acid modifications
	Modified residue	77	1	Phosphoserine Ref.9 Ref.12
	Modified residue	81	1	Phosphoserine Ref.9 Ref.12 Ref.8
	Modified residue	88	1	Phosphoserine Ref.9 Ref.12 Ref.8
	Modified residue	119	1	Phosphoserine By similarity
Natural variations
	Natural variant	36	1	P → H: dbSNP rs17857499. Ref.5		VAR_033203
	Natural variant	320	1	K → E: dbSNP rs17857501. Ref.5		VAR_033204
	Natural variant	556	1	G → S: dbSNP rs1051505. Ref.2 Ref.5		VAR_033205
Experimental info
	Mutagenesis	325	1	Y → A: Abolishes DNA-binding. Ref.10
	Mutagenesis	461	1	K → A: Abolishes nuclear targeting. Ref.11
	Mutagenesis	527	1	G → A or P: Impairs DNA-binding but not self-association. Ref.11
	Mutagenesis	530	1	Y → A: Impairs DNA-binding but not self-association. Ref.11
	Mutagenesis	530	1	Y → F: No effect on DNA-binding. Ref.11
	Mutagenesis	532	1	G → A: Impairs DNA-binding. Ref.11
	Mutagenesis	534	1	L → A: Impairs DNA-binding. Ref.11
	Sequence conflict	423	1	V → S in AAN74028. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q99856-1 [UniParc]. Last modified January 1, 1998. Version 2. Checksum: 4D09131E168A2880	FASTA	593	62,889
	10 20 30 40 50 60 MKLQAVMETL LQRQQRARQE LEARQQLPPD PPAAPPGRAR AAPDEDREPE SARMQRAQMA 70 80 90 100 110 120 ALAAMRAAAA GLGHPASPGG SEDGPPGSEE EDAAREGTPG SPGRGREGPG EEHFEDMASD 130 140 150 160 170 180 EDMKPKWEEE EMEEDLGEDE EEEEEDYEDE EEEEDEEGLG PPGPASLGTT ALFPRKAQPP 190 200 210 220 230 240 QAFRGDGVPR VLGGQERPGP GPAHPGGAAH VAPQLQPPDH GDWTYEEQFK QLYELDGDPK 250 260 270 280 290 300 RKEFLDDLFS FMQKRGTPVN RIPIMAKQVL DLFMLYVLVT EKGGLVEVIN KKLWREITKG 310 320 330 340 350 360 LNLPTSITSA AFTLRTQYMK YLYPYECEKR GLSNPNELQA AIDSNRREGR RQSFGGSLFA 370 380 390 400 410 420 YSPGGAHGML SSPKLPVSSL GLAASTNGSS ITPAPKIKKE EDSAIPITVP GRLPVSLAGH 430 440 450 460 470 480 PVVAAQAAAV QAAAAQAAVA AQAAALEQLR EKLESAEPPE KKMALVADEQ QRLMQRALQQ 490 500 510 520 530 540 NFLAMAAQLP MSIRINSQAS ESRQDSAVNL TGTNGSNSIS MSVEINGIMY TGVLFAQPPA 550 560 570 580 590 PTPTSAPNKG GGGGGGSSSN AGGRGGNTGT SGGQAGPAGL STPSTSTSNN SLP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The human dead ringer/bright homolog, DRIL1: cDNA cloning, gene structure, and mapping to D19S886, a marker on 19p13.3 that is strictly linked to the Peutz-Jeghers syndrome." Kortschak R.D., Reimann H., Zimmer M., Eyre H.J., Saint R., Jenne D.E. Genomics 51:288-292(1998) [PubMed: 9722953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]	"A novel E2F binding protein with Myc-type HLH motif stimulates E2F-dependent transcription by forming a heterodimer." Suzuki M., Okuyama S., Okamoto S., Shirasuna K., Nakajima T., Hachiya T., Nojima H., Sekiya S., Oda K. Oncogene 17:853-865(1998) [PubMed: 9780002] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH E2F1, VARIANT SER-556.
[3]	"Molecular cloning and immunologic characterization of DRIL3, a new bright-like ARID protein expressed in both myeloid and lymphoid cells, shares homology to E2FBP1/pRB family complexes." Paulin Y.G., Frank R.T., Davy E.J. Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Placenta.
[4]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed: 15057824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS HIS-36; GLU-320 AND SER-556. Tissue: Placenta.
[6]	"A functional screen identifies hDRIL1 as an oncogene that rescues RAS-induced senescence." Peeper D.S., Shvarts A., Brummelkamp T., Douma S., Koh E.Y., Daley G.Q., Bernards R. Nat. Cell Biol. 4:148-153(2002) [PubMed: 11812999] [Abstract] Cited for: FUNCTION.
[7]	"E2FBP1/DRIL1, an AT-rich interaction domain-family transcription factor, is regulated by p53." Ma K., Araki K., Ichwan S.J.A., Suganuma T., Tamamori-Adachi M., Ikeda M.-A. Mol. Cancer Res. 1:438-444(2003) [PubMed: 12692263] [Abstract] Cited for: FUNCTION, INDUCTION.
[8]	"Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-81 AND SER-88, MASS SPECTROMETRY. Tissue: Epithelium.
[9]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77; SER-81 AND SER-88, MASS SPECTROMETRY. Tissue: Epithelium.
[10]	"Induction of immunoglobulin heavy-chain transcription through the transcription factor Bright requires TFII-I." Rajaiya J., Nixon J.C., Ayers N., Desgranges Z.P., Roy A.L., Webb C.F. Mol. Cell. Biol. 26:4758-4768(2006) [PubMed: 16738337] [Abstract] Cited for: INTERACTION WITH GTF2I AND BTK, SUBUNIT, DNA-BINDING, MUTAGENESIS OF TYR-325.
[11]	"REKLES is an ARID3-restricted multifunctional domain." Kim D., Probst L., Das C., Tucker P.W. J. Biol. Chem. 282:15768-15777(2007) [PubMed: 17400556] [Abstract] Cited for: INTERACTION WITH ARID3B, DNA-BINDING, SUBUNIT, SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-461; GLY-527; TYR-530; GLY-532 AND LEU-534.
[12]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77; SER-81 AND SER-88, MASS SPECTROMETRY.
[13]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U88047 mRNA. Translation: AAC32888.1. AF039850 , AF039844, AF039845, AF039846, AF039847, AF039848, AF039849 Genomic DNA. Translation: AAC69994.1. AY152547 mRNA. Translation: AAN74028.1. AY845638 mRNA. Translation: AAW30734.1. Frameshift. AC005391 Genomic DNA. Translation: AAC28918.1. AC005379 Genomic DNA. Translation: AAC28499.1. BC033163 mRNA. Translation: AAH33163.1. BC060828 mRNA. Translation: AAH60828.1.
IPI	IPI00018500.
RefSeq	NP_005215.1.
UniGene	Hs.501296
3D structure databases
HSSP	HSSP built from PDB template 1C20 based on UniProtKB Q24573.
SMR	Q99856. Positions 223-349.
ModBase	Search...
PTM databases
PhosphoSite	Q99856.
Proteomic databases
PRIDE	Q99856.
Genome annotation databases
Ensembl	ENSG00000116017. Homo sapiens. [Contig view]
GeneID	1820.
KEGG	hsa:1820.
Organism-specific databases
GeneCards	GC19P000877.
H-InvDB	HIX0040051.
HGNC	HGNC:3031. ARID3A.
HPA	HPA004793.
MIM	603265. gene.
PharmGKB	PA27485.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q99856.
HOVERGEN	Q99856.
OMA	Q99856. PKWEEEE.
Gene expression databases
ArrayExpress	Q99856.
Bgee	Q99856.
CleanEx	HS_ARID3A.
GermOnline	ENSG00000116017. Homo sapiens.
Family and domain databases
InterPro	IPR001606. ARID. [Graphical view]
Gene3D	G3DSA:1.10.150.60. ARID. 1 hit.
Pfam	PF01388. ARID. 1 hit. [Graphical view]
SMART	SM00501. BRIGHT. 1 hit. [Graphical view]
PROSITE	PS51011. ARID. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	7417.
SOURCE	Search...

Entry information

Entry name

ARI3A_HUMAN

Accession

Primary (citable) accession number: Q99856
Secondary accession number(s): Q5I858 , Q6P9C6, Q8IZA7, Q8N4Z3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)