Q99832 (TCPH_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
T-complex protein 1 subunit eta
Short name=TCP-1-eta

Alternative name(s):
CCT-eta
HIV-1 Nef-interacting protein

Gene names

Name:	CCT7
Synonyms:	CCTH, NIP7-1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	543 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin By similarity.
Subunit structure	Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter. Interacts with PACRG. Ref.10
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the TCP-1 chaperonin family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Chaperone
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	'de novo' posttranslational protein folding Traceable author statement. Source: Reactome
Cellular_component	chaperonin-containing T-complex Inferred from electronic annotation. Source: Compara cytosol Traceable author statement. Source: Reactome microtubule Inferred from direct assay PubMed 21525035. Source: UniProtKB mitochondrion Inferred from electronic annotation. Source: Compara
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW unfolded protein binding Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q99832-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q99832-2)

The sequence of this isoform differs from the canonical sequence as follows:
     3-89: Missing.
     90-206: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q99832-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-44: Missing.

Note: No experimental confirmation available.

Isoform 4 (identifier: Q99832-4)

The sequence of this isoform differs from the canonical sequence as follows:
     3-89: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 543	543	T-complex protein 1 subunit eta		PRO_0000128365
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine Ref.7
	Modified residue	67	1	N6-acetyllysine Ref.11
	Modified residue	320	1	N6-acetyllysine Ref.11
Natural variations
	Alternative sequence	1 – 44	44	Missing in isoform 3.		VSP_043572
	Alternative sequence	3 – 89	87	Missing in isoform 2 and isoform 4.		VSP_043573
	Alternative sequence	90 – 206	117	Missing in isoform 2.		VSP_043574
	Natural variant	259	1	T → A. Corresponds to variant rs2231427 [ dbSNP | Ensembl ].		VAR_052269
Experimental info
	Sequence conflict	282 – 283	2	HH → RQ in AAB41437. Ref.8
	Sequence conflict	293	1	L → P in AAB41437. Ref.8
	Sequence conflict	336	1	A → P in AAB41437. Ref.8
	Sequence conflict	364	1	C → L in AAB41437. Ref.8
	Sequence conflict	374 – 376	3	LRG → SPC in AAB41437. Ref.8
	Sequence conflict	407	1	A → P in AAB41437. Ref.8
	Sequence conflict	411	1	A → P in AAB41437. Ref.8

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 15, 1998. Version 2. Checksum: 9F1E33FA80E6238E	FASTA	543	59,367
	10 20 30 40 50 60 MMPTPVILLK EGTDSSQGIP QLVSNISACQ VIAEAVRTTL GPRGMDKLIV DGRGKATISN 70 80 90 100 110 120 DGATILKLLD VVHPAAKTLV DIAKSQDAEV GDGTTSVTLL AAEFLKQVKP YVEEGLHPQI 130 140 150 160 170 180 IIRAFRTATQ LAVNKIKEIA VTVKKADKVE QRKLLEKCAM TALSSKLISQ QKAFFAKMVV 190 200 210 220 230 240 DAVMMLDDLL QLKMIGIKKV QGGALEDSQL VAGVAFKKTF SYAGFEMQPK KYHNPKIALL 250 260 270 280 290 300 NVELELKAEK DNAEIRVHTV EDYQAIVDAE WNILYDKLEK IHHSGAKVVL SKLPIGDVAT 310 320 330 340 350 360 QYFADRDMFC AGRVPEEDLK RTMMACGGSI QTSVNALSAD VLGRCQVFEE TQIGGERYNF 370 380 390 400 410 420 FTGCPKAKTC TFILRGGAEQ FMEETERSLH DAIMIVRRAI KNDSVVAGGG AIEMELSKYL 430 440 450 460 470 480 RDYSRTIPGK QQLLIGAYAK ALEIIPRQLC DNAGFDATNI LNKLRARHAQ GGTWYGVDIN 490 500 510 520 530 540 NEDIADNFEA FVWEPAMVRI NALTAASEAA CLIVSVDETI KNPRSTVDAP TAAGRGRGRG RPH
	Isoform 2 [UniParc]. Checksum: BA74E59CE7977306	FASTA	339	37,499
	10 20 30 40 50 60 MMDSQLVAGV AFKKTFSYAG FEMQPKKYHN PKIALLNVEL ELKAEKDNAE IRVHTVEDYQ 70 80 90 100 110 120 AIVDAEWNIL YDKLEKIHHS GAKVVLSKLP IGDVATQYFA DRDMFCAGRV PEEDLKRTMM 130 140 150 160 170 180 ACGGSIQTSV NALSADVLGR CQVFEETQIG GERYNFFTGC PKAKTCTFIL RGGAEQFMEE 190 200 210 220 230 240 TERSLHDAIM IVRRAIKNDS VVAGGGAIEM ELSKYLRDYS RTIPGKQQLL IGAYAKALEI 250 260 270 280 290 300 IPRQLCDNAG FDATNILNKL RARHAQGGTW YGVDINNEDI ADNFEAFVWE PAMVRINALT 310 320 330 AASEAACLIV SVDETIKNPR STVDAPTAAG RGRGRGRPH
	Isoform 3 [UniParc]. Checksum: 19F19C1820F6A80A	FASTA	499	54,804
	10 20 30 40 50 60 MDKLIVDGRG KATISNDGAT ILKLLDVVHP AAKTLVDIAK SQDAEVGDGT TSVTLLAAEF 70 80 90 100 110 120 LKQVKPYVEE GLHPQIIIRA FRTATQLAVN KIKEIAVTVK KADKVEQRKL LEKCAMTALS 130 140 150 160 170 180 SKLISQQKAF FAKMVVDAVM MLDDLLQLKM IGIKKVQGGA LEDSQLVAGV AFKKTFSYAG 190 200 210 220 230 240 FEMQPKKYHN PKIALLNVEL ELKAEKDNAE IRVHTVEDYQ AIVDAEWNIL YDKLEKIHHS 250 260 270 280 290 300 GAKVVLSKLP IGDVATQYFA DRDMFCAGRV PEEDLKRTMM ACGGSIQTSV NALSADVLGR 310 320 330 340 350 360 CQVFEETQIG GERYNFFTGC PKAKTCTFIL RGGAEQFMEE TERSLHDAIM IVRRAIKNDS 370 380 390 400 410 420 VVAGGGAIEM ELSKYLRDYS RTIPGKQQLL IGAYAKALEI IPRQLCDNAG FDATNILNKL 430 440 450 460 470 480 RARHAQGGTW YGVDINNEDI ADNFEAFVWE PAMVRINALT AASEAACLIV SVDETIKNPR 490 STVDAPTAAG RGRGRGRPH
	Isoform 4 [UniParc]. Checksum: AD52175094D0BD23	FASTA	456	50,352
	10 20 30 40 50 60 MMVGDGTTSV TLLAAEFLKQ VKPYVEEGLH PQIIIRAFRT ATQLAVNKIK EIAVTVKKAD 70 80 90 100 110 120 KVEQRKLLEK CAMTALSSKL ISQQKAFFAK MVVDAVMMLD DLLQLKMIGI KKVQGGALED 130 140 150 160 170 180 SQLVAGVAFK KTFSYAGFEM QPKKYHNPKI ALLNVELELK AEKDNAEIRV HTVEDYQAIV 190 200 210 220 230 240 DAEWNILYDK LEKIHHSGAK VVLSKLPIGD VATQYFADRD MFCAGRVPEE DLKRTMMACG 250 260 270 280 290 300 GSIQTSVNAL SADVLGRCQV FEETQIGGER YNFFTGCPKA KTCTFILRGG AEQFMEETER 310 320 330 340 350 360 SLHDAIMIVR RAIKNDSVVA GGGAIEMELS KYLRDYSRTI PGKQQLLIGA YAKALEIIPR 370 380 390 400 410 420 QLCDNAGFDA TNILNKLRAR HAQGGTWYGV DINNEDIADN FEAFVWEPAM VRINALTAAS 430 440 450 EAACLIVSVD ETIKNPRSTV DAPTAAGRGR GRGRPH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Maturation of human cyclin E requires the function of eukaryotic chaperonin CCT." Won K.-A., Schumacher R.J., Farr G.W., Horwich A.L., Reed S.I. Mol. Cell. Biol. 18:7584-7589(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2; 3 AND 4). Tissue: Cerebellum and Heart.
[4]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: PNS and Skin.
[7]	Bienvenut W.V., Glen H., Brunton V.G., Frame M.C., Boldt K., von Kriegsheim A.F., Kolch W. Submitted (DEC-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 1-10; 56-67; 85-123; 127-144; 178-193; 200-217; 219-230; 237-247; 293-306; 314-320; 376-397; 402-418; 431-447 AND 500-535, ACETYLATION AT MET-1, MASS SPECTROMETRY. Tissue: Colon carcinoma, Hepatoma and Osteosarcoma.
[8]	Fukushi M., Kimura T., Yamamoto N. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 13-424 (ISOFORM 1).
[9]	Lubec G., Afjehi-Sadat L. Submitted (MAR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 107-123, MASS SPECTROMETRY. Tissue: Brain and Cajal-Retzius cell.
[10]	"A product of the human gene adjacent to parkin is a component of Lewy bodies and suppresses Pael receptor-induced cell death." Imai Y., Soda M., Murakami T., Shoji M., Abe K., Takahashi R. J. Biol. Chem. 278:51901-51910(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PACRG.
[11]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-67 AND LYS-320, MASS SPECTROMETRY.
[12]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF026292 mRNA. Translation: AAC96011.1. CR536511 mRNA. Translation: CAG38749.1. AK291961 mRNA. Translation: BAF84650.1. AK293597 mRNA. Translation: BAH11543.1. AK297846 mRNA. Translation: BAH12675.1. AK298153 mRNA. Translation: BAH12733.1. AC010913 Genomic DNA. Translation: AAX88902.1. CH471053 Genomic DNA. Translation: EAW99739.1. CH471053 Genomic DNA. Translation: EAW99740.1. BC019296 mRNA. Translation: AAH19296.1. BC088351 mRNA. Translation: AAH88351.1. U83843 mRNA. Translation: AAB41437.1.
IPI	IPI00018465. IPI00952607. IPI00952671. IPI01014868.
RefSeq	NP_001009570.1. NM_001009570.2. NP_001159756.1. NM_001166284.1. NP_001159757.1. NM_001166285.1. NP_006420.1. NM_006429.3.
UniGene	Hs.368149.
3D structure databases
ProteinModelPortal	Q99832.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q99832. 36 interactions.
MINT	MINT-1131353.
STRING	9606.ENSP00000258091.
PTM databases
PhosphoSite	Q99832.
Polymorphism databases
DMDM	3041738.
2D gel databases
REPRODUCTION-2DPAGE	IPI00018465.
Proteomic databases
PaxDb	Q99832.
PRIDE	Q99832.
Protocols and materials databases
DNASU	10574.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000258091; ENSP00000258091; ENSG00000135624. ENST00000398422; ENSP00000381456; ENSG00000135624. ENST00000539919; ENSP00000437824; ENSG00000135624. ENST00000540468; ENSP00000442058; ENSG00000135624.
GeneID	10574.
KEGG	hsa:10574.
UCSC	uc002siz.3. human.
Organism-specific databases
CTD	10574.
GeneCards	GC02P073460.
HGNC	HGNC:1622. CCT7.
HPA	HPA008425.
MIM	605140. gene.
neXtProt	NX_Q99832.
PharmGKB	PA26185.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0459.
HOGENOM	HOG000226730.
HOVERGEN	HBG001052.
InParanoid	Q99832.
KO	K09499.
OMA	EKCAATA.
OrthoDB	EOG42821C.
Enzyme and pathway databases
Reactome	REACT_17015. Metabolism of proteins.
Gene expression databases
ArrayExpress	Q99832.
Bgee	Q99832.
CleanEx	HS_CCT7.
Genevestigator	Q99832.
GermOnline	ENSG00000135624. Homo sapiens.
Family and domain databases
InterPro	IPR012720. Chap_CCT_eta. IPR017998. Chaperone_TCP-1. IPR002194. Chaperonin_TCP-1_CS. IPR002423. Cpn60/TCP-1. [Graphical view]
PANTHER	PTHR11353. PTHR11353. 1 hit. PTHR11353:SF22. PTHR11353:SF22. 1 hit.
Pfam	PF00118. Cpn60_TCP1. 1 hit. [Graphical view]
PRINTS	PR00304. TCOMPLEXTCP1.
SUPFAM	SSF48592. GroEL-ATPase. 1 hit.
TIGRFAMs	TIGR02345. chap_CCT_eta. 1 hit.
PROSITE	PS00750. TCP1_1. 1 hit. PS00751. TCP1_2. 1 hit. PS00995. TCP1_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CCT7. human.
GenomeRNAi	10574.
NextBio	40131.
SOURCE	Search...

Entry information

Entry name

TCPH_HUMAN

Accession

Primary (citable) accession number: Q99832
Secondary accession number(s): A8K7E6 , A8MWI8, B7WNW9, B7Z4T9, B7Z4Z7, O14871, Q6FI26

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 15, 1998
Last modified:	May 1, 2013
This is version 132 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.