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Protein

Copine-1

Gene

CPNE1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-dependent phospholipid-binding protein that plays a role in calcium-mediated intracellular processes (PubMed:14674885). Involved in the TNF-alpha receptor signaling pathway in a calcium-dependent manner (PubMed:14674885). Exhibits calcium-dependent phospholipid binding properties (PubMed:9430674, PubMed:19539605). Plays a role in neuronal progenitor cell differentiation; induces neurite outgrowth via a AKT-dependent signaling cascade and calcium-independent manner (PubMed:23263657, PubMed:25450385). May recruit target proteins to the cell membrane in a calcium-dependent manner (PubMed:12522145). May function in membrane trafficking (PubMed:9430674). Involved in TNF-alpha-induced NF-kappa-B transcriptional repression by inducing endoprotease processing of the transcription factor NF-kappa-B p65/RELA subunit (PubMed:18212740). Also induces endoprotease processing of NF-kappa-B p50/NFKB1, p52/NFKB2, RELB and REL (PubMed:18212740).7 Publications

GO - Molecular functioni

  • calcium-dependent phospholipid binding Source: ProtInc
  • calcium ion binding Source: UniProtKB
  • endopeptidase activity Source: UniProtKB
  • NF-kappaB binding Source: UniProtKB
  • phosphatidylserine binding Source: MGI
  • protein homodimerization activity Source: UniProtKB
  • transporter activity Source: ProtInc

GO - Biological processi

  • cellular response to calcium ion Source: UniProtKB
  • glycerophospholipid biosynthetic process Source: Reactome
  • lipid metabolic process Source: ProtInc
  • negative regulation of DNA binding Source: UniProtKB
  • negative regulation of gene expression Source: UniProtKB
  • negative regulation of NIK/NF-kappaB signaling Source: UniProtKB
  • neuron projection extension Source: UniProtKB
  • positive regulation of neuron differentiation Source: UniProtKB
  • positive regulation of protein kinase B signaling Source: UniProtKB
  • positive regulation of tumor necrosis factor-mediated signaling pathway Source: UniProtKB
  • proteolysis Source: UniProtKB
  • regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
  • vesicle-mediated transport Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Differentiation, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-1483206. Glycerophospholipid biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Copine-1Curated
Alternative name(s):
Chromobindin 171 Publication
Copine I1 PublicationImported
Gene namesi
Name:CPNE1Imported
Synonyms:CPN1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

HGNCiHGNC:2314. CPNE1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • membrane Source: UniProtKB
  • nucleus Source: UniProtKB
  • plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi21 – 211D → A: Inhibits translocation to the cell membrane in a calcium-dependent manner and does not inhibit neuronal progenitor cell differentiation; when associated with A-90. 1 Publication
Mutagenesisi90 – 901D → A: Inhibits translocation to the cell membrane in a calcium-dependent manner and does not inhibit neuronal progenitor cell differentiation; when associated with A-21. 1 Publication
Mutagenesisi216 – 2161D → A: Inhibits translocation to the cell membrane in a calcium-dependent manner and does not inhibit neuronal progenitor cell differentiation; when associated with A-222. 1 Publication
Mutagenesisi222 – 2221D → A: Inhibits translocation to the cell membrane in a calcium-dependent manner and does not inhibit neuronal progenitor cell differentiation; when associated with A-216. 1 Publication

Organism-specific databases

PharmGKBiPA26831.

Polymorphism and mutation databases

BioMutaiCPNE1.
DMDMi10719953.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 537537Copine-1PRO_0000144834Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei171 – 1711N6-acetyllysineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ99829.
MaxQBiQ99829.
PaxDbiQ99829.
PeptideAtlasiQ99829.
PRIDEiQ99829.

PTM databases

iPTMnetiQ99829.
PhosphoSiteiQ99829.

Expressioni

Tissue specificityi

Expressed in neutrophils (at protein level) (PubMed:12949241). Widely expressed. Expressed in the brain. Expressed in neutrophil precursors from bone marrow and peripheral blood (PubMed:12949241).1 Publication

Inductioni

Up-regulated by the inflammatory cytokine TNF-alpha (PubMed:14674885). Up-regulated during neuronal progenitor cell differentiation (PubMed:23263657).2 Publications

Gene expression databases

BgeeiENSG00000214078.
CleanExiHS_CPN1.
ExpressionAtlasiQ99829. baseline and differential.
GenevisibleiQ99829. HS.

Organism-specific databases

HPAiHPA047259.

Interactioni

Subunit structurei

Homodimer; homodimerizes via its C2 domains (PubMed:18212740). Interacts with p65/RELA (via N-terminus); this interaction induces proteolytic cleavage of p65/RELA subunit and inhibition of NF-kappa-B transcriptional activity (PubMed:18212740). Interacts (via VWFA domain) with ACTB, CCDC22, MYCBP2, PPP5C, RDX and UBE2O (PubMed:12522145).2 Publications

GO - Molecular functioni

  • NF-kappaB binding Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB

Protein-protein interaction databases

BioGridi114418. 34 interactions.
IntActiQ99829. 6 interactions.
MINTiMINT-4999732.
STRINGi9606.ENSP00000317257.

Structurei

3D structure databases

ProteinModelPortaliQ99829.
SMRiQ99829. Positions 5-233.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 9898C2 1PROSITE-ProRule annotationAdd
BLAST
Domaini144 – 22885C2 2PROSITE-ProRule annotationAdd
BLAST
Domaini285 – 505221VWFAPROSITE-ProRule annotationAdd
BLAST

Domaini

C2 domains are necessary for calcium-dependent cell membrane association. C2 domains are necessary for neuronal progenitor cell differentiation in a calcium-independent manner (PubMed:25450385).1 Publication

Sequence similaritiesi

Belongs to the copine family.Curated
Contains 2 C2 domains.PROSITE-ProRule annotation
Contains 1 VWFA domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1327. Eukaryota.
ENOG410XPC8. LUCA.
GeneTreeiENSGT00760000119085.
HOGENOMiHOG000220898.
HOVERGENiHBG066841.
InParanoidiQ99829.
PhylomeDBiQ99829.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR010734. Copine.
IPR002035. VWF_A.
[Graphical view]
PfamiPF00168. C2. 2 hits.
PF07002. Copine. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 2 hits.
SM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
SSF53300. SSF53300. 1 hit.
PROSITEiPS50004. C2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99829-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHCVTLVQL SISCDHLIDK DIGSKSDPLC VLLQDVGGGS WAELGRTERV
60 70 80 90 100
RNCSSPEFSK TLQLEYRFET VQKLRFGIYD IDNKTPELRD DDFLGGAECS
110 120 130 140 150
LGQIVSSQVL TLPLMLKPGK PAGRGTITVS AQELKDNRVV TMEVEARNLD
160 170 180 190 200
KKDFLGKSDP FLEFFRQGDG KWHLVYRSEV IKNNLNPTWK RFSVPVQHFC
210 220 230 240 250
GGNPSTPIQV QCSDYDSDGS HDLIGTFHTS LAQLQAVPAE FECIHPEKQQ
260 270 280 290 300
KKKSYKNSGT IRVKICRVET EYSFLDYVMG GCQINFTVGV DFTGSNGDPS
310 320 330 340 350
SPDSLHYLSP TGVNEYLMAL WSVGSVVQDY DSDKLFPAFG FGAQVPPDWQ
360 370 380 390 400
VSHEFALNFN PSNPYCAGIQ GIVDAYRQAL PQVRLYGPTN FAPIINHVAR
410 420 430 440 450
FAAQAAHQGT ASQYFMLLLL TDGAVTDVEA TREAVVRASN LPMSVIIVGV
460 470 480 490 500
GGADFEAMEQ LDADGGPLHT RSGQAAARDI VQFVPYRRFQ NAPREALAQT
510 520 530
VLAEVPTQLV SYFRAQGWAP LKPLPPSAKD PAQAPQA
Length:537
Mass (Da):59,059
Last modified:May 1, 1997 - v1
Checksum:iF078D9046C2AAB06
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti332 – 3398SDKLFPAF → NSARAARV in AAG49297 (Ref. 6) Curated
Sequence conflicti367 – 3671A → V in CAG33071 (Ref. 2) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti211 – 2111Q → R.
Corresponds to variant rs6579255 [ dbSNP | Ensembl ].
VAR_024423
Natural varianti347 – 3471P → R.
Corresponds to variant rs12481228 [ dbSNP | Ensembl ].
VAR_048846
Natural varianti535 – 5351P → L.
Corresponds to variant rs11543239 [ dbSNP | Ensembl ].
VAR_048847

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U83246 mRNA. Translation: AAC15920.1.
CR456790 mRNA. Translation: CAG33071.1.
AL109827 Genomic DNA. Translation: CAB87610.2.
CH471077 Genomic DNA. Translation: EAW76173.1.
CH471077 Genomic DNA. Translation: EAW76174.1.
CH471077 Genomic DNA. Translation: EAW76175.1.
CH471077 Genomic DNA. Translation: EAW76177.1.
CH471077 Genomic DNA. Translation: EAW76178.1.
CH471077 Genomic DNA. Translation: EAW76179.1.
CH471077 Genomic DNA. Translation: EAW76183.1.
CH471077 Genomic DNA. Translation: EAW76185.1.
CH471077 Genomic DNA. Translation: EAW76186.1.
CH471077 Genomic DNA. Translation: EAW76187.1.
CH471077 Genomic DNA. Translation: EAW76189.1.
CH471077 Genomic DNA. Translation: EAW76190.1.
BC001142 mRNA. Translation: AAH01142.1.
AF314092 mRNA. Translation: AAG49297.1.
CCDSiCCDS13260.1.
RefSeqiNP_003906.2. NM_003915.5.
NP_690902.1. NM_152925.2.
NP_690903.1. NM_152926.2.
NP_690904.1. NM_152927.2.
NP_690905.1. NM_152928.2.
UniGeneiHs.246413.
Hs.700188.

Genome annotation databases

EnsembliENST00000352393; ENSP00000336945; ENSG00000214078.
ENST00000397443; ENSP00000380585; ENSG00000214078.
GeneIDi8904.
KEGGihsa:8904.
UCSCiuc002xdf.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U83246 mRNA. Translation: AAC15920.1.
CR456790 mRNA. Translation: CAG33071.1.
AL109827 Genomic DNA. Translation: CAB87610.2.
CH471077 Genomic DNA. Translation: EAW76173.1.
CH471077 Genomic DNA. Translation: EAW76174.1.
CH471077 Genomic DNA. Translation: EAW76175.1.
CH471077 Genomic DNA. Translation: EAW76177.1.
CH471077 Genomic DNA. Translation: EAW76178.1.
CH471077 Genomic DNA. Translation: EAW76179.1.
CH471077 Genomic DNA. Translation: EAW76183.1.
CH471077 Genomic DNA. Translation: EAW76185.1.
CH471077 Genomic DNA. Translation: EAW76186.1.
CH471077 Genomic DNA. Translation: EAW76187.1.
CH471077 Genomic DNA. Translation: EAW76189.1.
CH471077 Genomic DNA. Translation: EAW76190.1.
BC001142 mRNA. Translation: AAH01142.1.
AF314092 mRNA. Translation: AAG49297.1.
CCDSiCCDS13260.1.
RefSeqiNP_003906.2. NM_003915.5.
NP_690902.1. NM_152925.2.
NP_690903.1. NM_152926.2.
NP_690904.1. NM_152927.2.
NP_690905.1. NM_152928.2.
UniGeneiHs.246413.
Hs.700188.

3D structure databases

ProteinModelPortaliQ99829.
SMRiQ99829. Positions 5-233.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114418. 34 interactions.
IntActiQ99829. 6 interactions.
MINTiMINT-4999732.
STRINGi9606.ENSP00000317257.

PTM databases

iPTMnetiQ99829.
PhosphoSiteiQ99829.

Polymorphism and mutation databases

BioMutaiCPNE1.
DMDMi10719953.

Proteomic databases

EPDiQ99829.
MaxQBiQ99829.
PaxDbiQ99829.
PeptideAtlasiQ99829.
PRIDEiQ99829.

Protocols and materials databases

DNASUi8904.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000352393; ENSP00000336945; ENSG00000214078.
ENST00000397443; ENSP00000380585; ENSG00000214078.
GeneIDi8904.
KEGGihsa:8904.
UCSCiuc002xdf.4. human.

Organism-specific databases

CTDi8904.
GeneCardsiCPNE1.
HGNCiHGNC:2314. CPNE1.
HPAiHPA047259.
MIMi604205. gene.
neXtProtiNX_Q99829.
PharmGKBiPA26831.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1327. Eukaryota.
ENOG410XPC8. LUCA.
GeneTreeiENSGT00760000119085.
HOGENOMiHOG000220898.
HOVERGENiHBG066841.
InParanoidiQ99829.
PhylomeDBiQ99829.

Enzyme and pathway databases

ReactomeiR-HSA-1483206. Glycerophospholipid biosynthesis.

Miscellaneous databases

ChiTaRSiCPNE1. human.
GeneWikiiCPNE1.
GenomeRNAii8904.
PROiQ99829.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000214078.
CleanExiHS_CPN1.
ExpressionAtlasiQ99829. baseline and differential.
GenevisibleiQ99829. HS.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR010734. Copine.
IPR002035. VWF_A.
[Graphical view]
PfamiPF00168. C2. 2 hits.
PF07002. Copine. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 2 hits.
SM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
SSF53300. SSF53300. 1 hit.
PROSITEiPS50004. C2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCPNE1_HUMAN
AccessioniPrimary (citable) accession number: Q99829
Secondary accession number(s): E1P5Q4
, Q6IBL3, Q9H243, Q9NTZ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1997
Last modified: September 7, 2016
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.