Reviewed, UniProtKB/Swiss-Prot Q99808 (S29A1_HUMAN)

Names and origin

Protein names

Recommended name:
    Equilibrative nucleoside transporter 1
Alternative name(s):
    Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter
      Short name=Equilibrative NBMPR-sensitive nucleoside transporter
    Nucleoside transporter, es-type
    Solute carrier family 29 member 1

Gene names

Name:	SLC29A1
Synonyms:	ENT1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	456 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Inhibited by dipyridamole and dilazep (anticancer chemotherapeutics drugs).
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Expressed in heart, brain, mammary gland, erythrocytes and placenta, and also in fetal liver and spleen.
Post-translational modification	Glycosylated. Ref.9
Sequence similarities	Belongs to the SLC29A transporter family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	nucleobase, nucleoside, nucleotide and nucleic acid metabolic process Ref.1 Traceable author statement. Source: ProtInc nucleoside transport Ref.1 Traceable author statement. Source: ProtInc
Cellular component	integral to plasma membrane Ref.1 Traceable author statement. Source: ProtInc membrane fraction Ref.1 Traceable author statement. Source: ProtInc
Molecular function	nucleoside transmembrane transporter activity Ref.1 Inferred from Experiment. Source: Reactome protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
STK19	P49842	1	EBI-347907,EBI-347581

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 456	455	Equilibrative nucleoside transporter 1		PRO_0000209337
Regions
	Topological domain	2 – 12	11	Cytoplasmic Potential
	Transmembrane	13 – 29	17	Potential
	Topological domain	30 – 82	53	Extracellular Potential
	Transmembrane	83 – 107	25	Potential
	Topological domain	108 – 111	4	Cytoplasmic Potential
	Transmembrane	112 – 130	19	Potential
	Topological domain	131 – 138	8	Extracellular Potential
	Transmembrane	139 – 157	19	Potential
	Topological domain	158 – 174	17	Cytoplasmic Potential
	Transmembrane	175 – 199	25	Potential
	Topological domain	200 – 206	7	Extracellular Potential
	Transmembrane	207 – 227	21	Potential
	Topological domain	228 – 291	64	Cytoplasmic Potential
	Transmembrane	292 – 311	20	Potential
	Topological domain	312 – 323	12	Extracellular Potential
	Transmembrane	324 – 342	19	Potential
	Topological domain	343 – 359	17	Cytoplasmic Potential
	Transmembrane	360 – 378	19	Potential
	Topological domain	379 – 393	15	Extracellular Potential
	Transmembrane	394 – 413	20	Potential
	Topological domain	414 – 431	18	Cytoplasmic Potential
	Transmembrane	432 – 452	21	Potential
	Topological domain	453 – 456	4	Extracellular Potential
Amino acid modifications
	Modified residue	254	1	Phosphoserine Ref.8
	Glycosylation	48	1	N-linked (GlcNAc...) Ref.9
Natural variations
	Natural variant	216	1	I → T: dbSNP rs45573936.		VAR_053668
	Natural variant	293	1	A → T in a colorectal cancer sample; somatic mutation. Ref.10		VAR_036221
	Natural variant	391	1	E → K: dbSNP rs45458701.		VAR_053669
	Natural variant	455	1	I → V in a colorectal cancer sample; somatic mutation. Ref.10		VAR_036222

Sequences

	Sequence		Length	Mass (Da)
	Q99808-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: F18535A95DEBC95D	FASTA	456	50,219
	10 20 30 40 50 60 MTTSHQPQDR YKAVWLIFFM LGLGTLLPWN FFMTATQYFT NRLDMSQNVS LVTAELSKDA 70 80 90 100 110 120 QASAAPAAPL PERNSLSAIF NNVMTLCAML PLLLFTYLNS FLHQRIPQSV RILGSLVAIL 130 140 150 160 170 180 LVFLITAILV KVQLDALPFF VITMIKIVLI NSFGAILQGS LFGLAGLLPA SYTAPIMSGQ 190 200 210 220 230 240 GLAGFFASVA MICAIASGSE LSESAFGYFI TACAVIILTI ICYLGLPRLE FYRYYQQLKL 250 260 270 280 290 300 EGPGEQETKL DLISKGEEPR AGKEESGVSV SNSQPTNESH SIKAILKNIS VLAFSVCFIF 310 320 330 340 350 360 TITIGMFPAV TVEVKSSIAG SSTWERYFIP VSCFLTFNIF DWLGRSLTAV FMWPGKDSRW 370 380 390 400 410 420 LPSLVLARLV FVPLLLLCNI KPRRYLTVVF EHDAWFIFFM AAFAFSNGYL ASLCMCFGPK 430 440 450 KVKPAEAETA GAIMAFFLCL GLALGAVFSF LFRAIV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a human nucleoside transporter implicated in the cellular uptake of adenosine and chemotherapeutic drugs." Griffiths M., Beaumont N., Yao S.Y.M., Sundaram M., Boumah C.E., Davies A., Kwong F.Y.P., Coe I., Cass C.E., Young J.D., Baldwin S.A. Nat. Med. 3:89-93(1997) [PubMed: 8986748] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-22. Tissue: Placenta.
[2]	"Genomic sequence of the human equilibrative nucleoside transporter 1 (hENT1)." Graham K.A., Coe I.R., Carpenter P., Baldwin S.A., Young J.D., Cass C.E. Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Human intestinal es nucleoside transporter: molecular characterization and nucleoside inhibitory profiles." Lum P.Y., Ngo L.Y., Bakken A.H., Unadkat J.D. Cancer Chemother. Pharmacol. 45:273-278(2000) [PubMed: 10755314] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Jejunum and Small intestine.
[4]	"Comparative genomic analysis of equilibrative nucleoside transporters suggests conserved protein structure despite limited sequence identity." Sankar N., Machado J., Abdulla P., Hilliker A.J., Coe I.R. Nucleic Acids Res. 30:4339-4350(2002) [PubMed: 12384580] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed: 14574404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon and Muscle.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-254, MASS SPECTROMETRY.
[9]	"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins." Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D. Nat. Biotechnol. 27:378-386(2009) [PubMed: 19349973] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-48, MASS SPECTROMETRY.
[10]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed: 16959974] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] THR-293 AND VAL-455.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U81375 mRNA. Translation: AAC51103.1. AF190884 Genomic DNA. Translation: AAF02777.1. AF079117 mRNA. Translation: AAC62495.1. AF495730 Genomic DNA. Translation: AAM11785.1. AK090615 mRNA. Translation: BAG52197.1. AL139392 Genomic DNA. Translation: CAI20093.1. BC001382 mRNA. Translation: AAH01382.1. BC008954 mRNA. Translation: AAH08954.1.
IPI	IPI00939219.
RefSeq	NP_001071642.1. NP_001071643.1. NP_001071644.1. NP_001071645.1. NP_004946.1.
UniGene	Hs.25450
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q99808. 1 interaction.
STRING	Q99808.
Protein family/group databases
TCDB	2.A.57.1.1. equilibrative nucleoside transporter (ENT) family.
PTM databases
PhosphoSite	Q99808.
Proteomic databases
PRIDE	Q99808.
Genome annotation databases
Ensembl	ENST00000313248; ENSP00000319152; ENSG00000112759; Homo sapiens. [Genome view] ENST00000371708; ENSP00000360773; ENSG00000112759; Homo sapiens. [Genome view] ENST00000371713; ENSP00000360778; ENSG00000112759; Homo sapiens. [Genome view] ENST00000371724; ENSP00000360789; ENSG00000112759; Homo sapiens. [Genome view] ENST00000371731; ENSP00000360796; ENSG00000112759; Homo sapiens. [Genome view] ENST00000371740; ENSP00000360805; ENSG00000112759; Homo sapiens. [Genome view] ENST00000371755; ENSP00000360820; ENSG00000112759; Homo sapiens. [Genome view] ENST00000393841; ENSP00000377424; ENSG00000112759; Homo sapiens. [Genome view] ENST00000393844; ENSP00000377427; ENSG00000112759; Homo sapiens. [Genome view] ENST00000427851; ENSP00000392668; ENSG00000112759; Homo sapiens. [Genome view]
GeneID	2030.
KEGG	hsa:2030.
UCSC	uc003owu.1. human.
Organism-specific databases
CTD	2030.
GeneCards	GC06P044295.
H-InvDB	HIX0005921.
HGNC	HGNC:11003. SLC29A1.
HPA	HPA012383. HPA012384.
MIM	602193. gene.
PharmGKB	PA154.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q99808.
InParanoid	Q99808.
OrthoDB	EOG93N9Z1.
Enzyme and pathway databases
Reactome	REACT_15518. Transmembrane transport of small molecules. REACT_1698. Metablism of nucleotides.
Gene expression databases
ArrayExpress	Q99808.
Bgee	Q99808.
CleanEx	HS_SLC29A1.
Genevestigator	Q99808.
GermOnline	ENSG00000112759. Homo sapiens.
Family and domain databases
InterPro	IPR002259. DER/eqnu_transpt. [Graphical view]
PANTHER	PTHR10332. DER/eqnu_transpt. 1 hit.
Pfam	PF01733. Nucleoside_tran. 1 hit. [Graphical view]
PRINTS	PR01130. DERENTRNSPRT.
TIGRFAMs	TIGR00939. 2a57. 1 hit.
ProtoNet	Search...
Other Resources
DrugBank	DB00197. Troglitazone.
NextBio	8235.
SOURCE	Search...

Entry information

Entry name

S29A1_HUMAN

Accession

Primary (citable) accession number: Q99808
Secondary accession number(s): B3KQY5, Q5T9W9, Q9UJY2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	January 23, 2007
Last modified:	December 15, 2009
This is version 89 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.