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Q99808 (S29A1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 132. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Equilibrative nucleoside transporter 1
Alternative name(s):
Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter
Short name=Equilibrative NBMPR-sensitive nucleoside transporter
Nucleoside transporter, es-type
Solute carrier family 29 member 1
Gene names
Name:SLC29A1
Synonyms:ENT1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length456 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Inhibited by dipyridamole and dilazep (anticancer chemotherapeutics drugs).

Subunit structure

Identified in a complex with STOM. Ref.11

Subcellular location

Basolateral cell membrane; Multi-pass membrane protein. Apical cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Note: Predominantly localized in the basolateral membrane in polarized MDCK cells. Ref.5 Ref.11

Tissue specificity

Detected in erythrocytes (at protein level). Expressed in heart, brain, mammary gland, erythrocytes and placenta, and also in fetal liver and spleen. Ref.5 Ref.11

Post-translational modification

Glycosylated.

Sequence similarities

Belongs to the SLC29A/ENT transporter (TC 2.A.57) family. [View classification]

Biophysicochemical properties

Kinetic parameters:

Vmax for adenosine uptake is about the same for SLC29A1 and SLC29A2.

KM=0.62 mM for adenosine Ref.5

Ontologies

Keywords
   Biological processTransport
   Cellular componentCell membrane
Membrane
   Coding sequence diversityPolymorphism
   DomainTransmembrane
Transmembrane helix
   PTMGlycoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processcellular response to glucose stimulus

Inferred from electronic annotation. Source: Ensembl

cellular response to hypoxia

Inferred from electronic annotation. Source: Ensembl

lactation

Inferred from electronic annotation. Source: Ensembl

nucleobase-containing compound metabolic process

Traceable author statement Ref.1. Source: ProtInc

nucleoside transmembrane transport

Traceable author statement Ref.1. Source: GOC

nucleoside transport

Inferred from direct assay Ref.5. Source: UniProtKB

regulation of excitatory postsynaptic membrane potential

Inferred from electronic annotation. Source: Ensembl

sleep

Inferred from electronic annotation. Source: Ensembl

transmembrane transport

Traceable author statement. Source: Reactome

uridine transport

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentapical plasma membrane

Inferred from direct assay Ref.5. Source: UniProtKB

basolateral plasma membrane

Inferred from direct assay Ref.5. Source: UniProtKB

integral component of plasma membrane

Traceable author statement Ref.1. Source: ProtInc

membrane

Traceable author statement Ref.1. Source: ProtInc

plasma membrane

Traceable author statement. Source: Reactome

   Molecular_functionnucleoside transmembrane transporter activity

Traceable author statement Ref.1. Source: ProtInc

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

STK19P498421EBI-347907,EBI-347581

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1
Chain2 – 456455Equilibrative nucleoside transporter 1
PRO_0000209337

Regions

Topological domain2 – 1211Cytoplasmic Potential
Transmembrane13 – 2917Helical; Potential
Topological domain30 – 8253Extracellular Potential
Transmembrane83 – 10725Helical; Potential
Topological domain108 – 1114Cytoplasmic Potential
Transmembrane112 – 13019Helical; Potential
Topological domain131 – 1388Extracellular Potential
Transmembrane139 – 15719Helical; Potential
Topological domain158 – 17417Cytoplasmic Potential
Transmembrane175 – 19925Helical; Potential
Topological domain200 – 2067Extracellular Potential
Transmembrane207 – 22721Helical; Potential
Topological domain228 – 29164Cytoplasmic Potential
Transmembrane292 – 31120Helical; Potential
Topological domain312 – 32312Extracellular Potential
Transmembrane324 – 34219Helical; Potential
Topological domain343 – 35917Cytoplasmic Potential
Transmembrane360 – 37819Helical; Potential
Topological domain379 – 39315Extracellular Potential
Transmembrane394 – 41320Helical; Potential
Topological domain414 – 43118Cytoplasmic Potential
Transmembrane432 – 45221Helical; Potential
Topological domain453 – 4564Extracellular Potential

Amino acid modifications

Glycosylation481N-linked (GlcNAc...) Ref.10

Natural variations

Natural variant2161I → T.
Corresponds to variant rs45573936 [ dbSNP | Ensembl ].
VAR_053668
Natural variant2931A → T in a colorectal cancer sample; somatic mutation. Ref.12
VAR_036221
Natural variant3911E → K.
Corresponds to variant rs45458701 [ dbSNP | Ensembl ].
VAR_053669
Natural variant4551I → V in a colorectal cancer sample; somatic mutation. Ref.12
VAR_036222

Experimental info

Mutagenesis453 – 4564Missing: No effect on subcellular localization and inosine transport. Ref.5
Mutagenesis4531R → A: No effect on subcellular localization and inosine transport. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Q99808 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: F18535A95DEBC95D

FASTA45650,219
        10         20         30         40         50         60 
MTTSHQPQDR YKAVWLIFFM LGLGTLLPWN FFMTATQYFT NRLDMSQNVS LVTAELSKDA 

        70         80         90        100        110        120 
QASAAPAAPL PERNSLSAIF NNVMTLCAML PLLLFTYLNS FLHQRIPQSV RILGSLVAIL 

       130        140        150        160        170        180 
LVFLITAILV KVQLDALPFF VITMIKIVLI NSFGAILQGS LFGLAGLLPA SYTAPIMSGQ 

       190        200        210        220        230        240 
GLAGFFASVA MICAIASGSE LSESAFGYFI TACAVIILTI ICYLGLPRLE FYRYYQQLKL 

       250        260        270        280        290        300 
EGPGEQETKL DLISKGEEPR AGKEESGVSV SNSQPTNESH SIKAILKNIS VLAFSVCFIF 

       310        320        330        340        350        360 
TITIGMFPAV TVEVKSSIAG SSTWERYFIP VSCFLTFNIF DWLGRSLTAV FMWPGKDSRW 

       370        380        390        400        410        420 
LPSLVLARLV FVPLLLLCNI KPRRYLTVVF EHDAWFIFFM AAFAFSNGYL ASLCMCFGPK 

       430        440        450 
KVKPAEAETA GAIMAFFLCL GLALGAVFSF LFRAIV 

« Hide

References

« Hide 'large scale' references
[1]"Cloning of a human nucleoside transporter implicated in the cellular uptake of adenosine and chemotherapeutic drugs."
Griffiths M., Beaumont N., Yao S.Y.M., Sundaram M., Boumah C.E., Davies A., Kwong F.Y.P., Coe I., Cass C.E., Young J.D., Baldwin S.A.
Nat. Med. 3:89-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-22.
Tissue: Placenta.
[2]"Genomic sequence of the human equilibrative nucleoside transporter 1 (hENT1)."
Graham K.A., Coe I.R., Carpenter P., Baldwin S.A., Young J.D., Cass C.E.
Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Human intestinal es nucleoside transporter: molecular characterization and nucleoside inhibitory profiles."
Lum P.Y., Ngo L.Y., Bakken A.H., Unadkat J.D.
Cancer Chemother. Pharmacol. 45:273-278(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Jejunum and Small intestine.
[4]"Comparative genomic analysis of equilibrative nucleoside transporters suggests conserved protein structure despite limited sequence identity."
Sankar N., Machado J., Abdulla P., Hilliker A.J., Coe I.R.
Nucleic Acids Res. 30:4339-4350(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"Localization of human equilibrative nucleoside transporters, hENT1 and hENT2, in renal epithelial cells."
Mangravite L.M., Xiao G., Giacomini K.M.
Am. J. Physiol. 284:F902-F910(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF ARG-453 AND 453-ARG--VAL-456.
[6]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]"The DNA sequence and analysis of human chromosome 6."
Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Colon and Muscle.
[9]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[10]"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-48.
Tissue: Leukemic T-cell.
[11]"Stomatin interacts with GLUT1/SLC2A1, band 3/SLC4A1, and aquaporin-1 in human erythrocyte membrane domains."
Rungaldier S., Oberwagner W., Salzer U., Csaszar E., Prohaska R.
Biochim. Biophys. Acta 1828:956-966(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, IDENTIFICATION IN A COMPLEX WITH STOM, SUBUNIT.
[12]"The consensus coding sequences of human breast and colorectal cancers."
Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. expand/collapse author list , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.
Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANTS [LARGE SCALE ANALYSIS] THR-293 AND VAL-455.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U81375 mRNA. Translation: AAC51103.1.
AF190884 Genomic DNA. Translation: AAF02777.1.
AF079117 mRNA. Translation: AAC62495.1.
AF495730 Genomic DNA. Translation: AAM11785.1.
AK090615 mRNA. Translation: BAG52197.1.
AL139392 Genomic DNA. Translation: CAI20093.1.
BC001382 mRNA. Translation: AAH01382.1.
BC008954 mRNA. Translation: AAH08954.1.
CCDSCCDS4908.1.
RefSeqNP_001071642.1. NM_001078174.1.
NP_001071643.1. NM_001078175.2.
NP_001071644.1. NM_001078176.2.
NP_001071645.1. NM_001078177.1.
NP_004946.1. NM_004955.2.
XP_005248935.1. XM_005248878.2.
XP_005248936.1. XM_005248879.2.
XP_005248937.1. XM_005248880.2.
XP_005248938.1. XM_005248881.2.
XP_005248939.1. XM_005248882.2.
UniGeneHs.25450.

3D structure databases

ProteinModelPortalQ99808.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid108344. 2 interactions.
IntActQ99808. 3 interactions.
MINTMINT-1034227.
STRING9606.ENSP00000360773.

Chemistry

BindingDBQ99808.
ChEMBLCHEMBL1997.
DrugBankDB00197. Troglitazone.
GuidetoPHARMACOLOGY1117.

Protein family/group databases

TCDB2.A.57.1.1. the equilibrative nucleoside transporter (ent) family.

PTM databases

PhosphoSiteQ99808.

Polymorphism databases

DMDM9296956.

Proteomic databases

MaxQBQ99808.
PaxDbQ99808.
PRIDEQ99808.

Protocols and materials databases

DNASU2030.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000371708; ENSP00000360773; ENSG00000112759.
ENST00000371713; ENSP00000360778; ENSG00000112759.
ENST00000371724; ENSP00000360789; ENSG00000112759.
ENST00000371731; ENSP00000360796; ENSG00000112759.
ENST00000371740; ENSP00000360805; ENSG00000112759.
ENST00000371755; ENSP00000360820; ENSG00000112759.
ENST00000393841; ENSP00000377424; ENSG00000112759.
ENST00000393844; ENSP00000377427; ENSG00000112759.
ENST00000427851; ENSP00000392668; ENSG00000112759.
GeneID2030.
KEGGhsa:2030.
UCSCuc003owu.1. human.

Organism-specific databases

CTD2030.
GeneCardsGC06P044187.
HGNCHGNC:11003. SLC29A1.
HPAHPA012383.
HPA012384.
MIM602193. gene.
neXtProtNX_Q99808.
PharmGKBPA154.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG249415.
HOGENOMHOG000007684.
HOVERGENHBG074626.
InParanoidQ99808.
KOK15014.
OrthoDBEOG7PP56W.
PhylomeDBQ99808.
TreeFamTF313950.

Enzyme and pathway databases

ReactomeREACT_15518. Transmembrane transport of small molecules.

Gene expression databases

ArrayExpressQ99808.
BgeeQ99808.
CleanExHS_SLC29A1.
GenevestigatorQ99808.

Family and domain databases

InterProIPR002259. Eqnu_transpt.
IPR016196. MFS_dom_general_subst_transpt.
[Graphical view]
PANTHERPTHR10332. PTHR10332. 1 hit.
PfamPF01733. Nucleoside_tran. 1 hit.
[Graphical view]
PIRSFPIRSF016379. ENT. 1 hit.
PRINTSPR01130. DERENTRNSPRT.
SUPFAMSSF103473. SSF103473. 3 hits.
TIGRFAMsTIGR00939. 2a57. 1 hit.
ProtoNetSearch...

Other

GeneWikiEquilibrative_nucleoside_transporter_1.
GenomeRNAi2030.
NextBio8235.
PROQ99808.
SOURCESearch...

Entry information

Entry nameS29A1_HUMAN
AccessionPrimary (citable) accession number: Q99808
Secondary accession number(s): B3KQY5, Q5T9W9, Q9UJY2
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 132 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 6

Human chromosome 6: entries, gene names and cross-references to MIM