ID   GPA33_HUMAN             Reviewed;         319 AA.
AC   Q99795; Q5VZP6;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   27-MAR-2024, entry version 194.
DE   RecName: Full=Cell surface A33 antigen;
DE   AltName: Full=Glycoprotein A33;
DE   Flags: Precursor;
GN   Name=GPA33;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
RC   TISSUE=Colon carcinoma;
RX   PubMed=9012807; DOI=10.1073/pnas.94.2.469;
RA   Heath J.K., White S.J., Johnstone C.N., Catimel B., Simpson R.J.,
RA   Moritz R.L., Tu G.-F., Ji H., Whitehead R.H., Groenen L.C., Scott A.M.,
RA   Ritter G., Cohen L., Welt S., Old L.J., Nice E.C., Burgess A.W.;
RT   "The human A33 antigen is a transmembrane glycoprotein and a novel member
RT   of the immunoglobulin superfamily.";
RL   Proc. Natl. Acad. Sci. U.S.A. 94:469-474(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Thymus;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16710414; DOI=10.1038/nature04727;
RA   Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A.,
RA   Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C.,
RA   Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.,
RA   Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C.,
RA   Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W.,
RA   Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J.,
RA   Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J.,
RA   Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y.,
RA   Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J.,
RA   Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA   Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA   Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA   Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S.,
RA   Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K.,
RA   Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R.,
RA   Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M.,
RA   Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S.,
RA   Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J.,
RA   Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W.,
RA   McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA   Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA   Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA   Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA   Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S.,
RA   Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M.,
RA   White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H.,
RA   Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E.,
RA   Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G.,
RA   Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
RT   "The DNA sequence and biological annotation of human chromosome 1.";
RL   Nature 441:315-321(2006).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Lung;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [6]
RP   GLYCOSYLATION AT ASN-112, AND PALMITOYLATION.
RX   PubMed=9245713; DOI=10.1006/bbrc.1997.6966;
RA   Ritter G., Cohen L.S., Nice E.C., Catimel B., Burgess A.W., Moritz R.L.,
RA   Ji H., Heath J.K., White S.J., Welt S., Old L.J., Simpson R.J.;
RT   "Characterization of posttranslational modifications of human A33 antigen,
RT   a novel palmitoylated surface glycoprotein of human gastrointestinal
RT   epithelium.";
RL   Biochem. Biophys. Res. Commun. 236:682-686(1997).
CC   -!- FUNCTION: May play a role in cell-cell recognition and signaling.
CC   -!- INTERACTION:
CC       Q99795; P54852: EMP3; NbExp=3; IntAct=EBI-4289554, EBI-3907816;
CC       Q99795; O75355-2: ENTPD3; NbExp=3; IntAct=EBI-4289554, EBI-12279764;
CC       Q99795; Q13021: MALL; NbExp=3; IntAct=EBI-4289554, EBI-750078;
CC       Q99795; Q8IZ57: NRSN1; NbExp=3; IntAct=EBI-4289554, EBI-10264528;
CC       Q99795; P42857: NSG1; NbExp=3; IntAct=EBI-4289554, EBI-6380741;
CC       Q99795; Q9NUX5: POT1; NbExp=2; IntAct=EBI-4289554, EBI-752420;
CC       Q99795; Q9NRQ5: SMCO4; NbExp=3; IntAct=EBI-4289554, EBI-8640191;
CC       Q99795; O00526: UPK2; NbExp=3; IntAct=EBI-4289554, EBI-10179682;
CC   -!- SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
CC   -!- TISSUE SPECIFICITY: Expressed in normal gastrointestinal epithelium and
CC       in 95% of colon cancers.
CC   -!- PTM: N-glycosylated, contains approximately 8 kDa of N-linked
CC       carbohydrate. {ECO:0000269|PubMed:9245713}.
CC   -!- PTM: Palmitoylated. {ECO:0000269|PubMed:9245713}.
CC   -!- WEB RESOURCE: Name=Atlas of Genetics and Cytogenetics in Oncology and
CC       Haematology;
CC       URL="https://atlasgeneticsoncology.org/gene/40735/GPA33";
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DR   EMBL; U79725; AAC50957.1; -; mRNA.
DR   EMBL; AK312833; BAG35687.1; -; mRNA.
DR   EMBL; AL158837; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CH471067; EAW90783.1; -; Genomic_DNA.
DR   EMBL; BC069705; AAH69705.1; -; mRNA.
DR   EMBL; BC069723; AAH69723.1; -; mRNA.
DR   EMBL; BC069745; AAH69745.1; -; mRNA.
DR   EMBL; BC069761; AAH69761.1; -; mRNA.
DR   EMBL; BC069789; AAH69789.1; -; mRNA.
DR   EMBL; BC074830; AAH74830.1; -; mRNA.
DR   EMBL; BC074876; AAH74876.1; -; mRNA.
DR   EMBL; BC107164; AAI07165.1; -; mRNA.
DR   EMBL; BC107165; AAI07166.1; -; mRNA.
DR   CCDS; CCDS1258.1; -.
DR   RefSeq; NP_005805.1; NM_005814.2.
DR   AlphaFoldDB; Q99795; -.
DR   SMR; Q99795; -.
DR   BioGRID; 115517; 17.
DR   IntAct; Q99795; 8.
DR   STRING; 9606.ENSP00000356842; -.
DR   ChEMBL; CHEMBL3712927; -.
DR   GlyCosmos; Q99795; 3 sites, No reported glycans.
DR   GlyGen; Q99795; 4 sites, 1 O-linked glycan (1 site).
DR   iPTMnet; Q99795; -.
DR   PhosphoSitePlus; Q99795; -.
DR   SwissPalm; Q99795; -.
DR   BioMuta; GPA33; -.
DR   DMDM; 2842765; -.
DR   jPOST; Q99795; -.
DR   MassIVE; Q99795; -.
DR   MaxQB; Q99795; -.
DR   PaxDb; 9606-ENSP00000356842; -.
DR   PeptideAtlas; Q99795; -.
DR   ProteomicsDB; 78476; -.
DR   ABCD; Q99795; 5 sequenced antibodies.
DR   Antibodypedia; 1116; 404 antibodies from 31 providers.
DR   DNASU; 10223; -.
DR   Ensembl; ENST00000367868.4; ENSP00000356842.3; ENSG00000143167.12.
DR   GeneID; 10223; -.
DR   KEGG; hsa:10223; -.
DR   MANE-Select; ENST00000367868.4; ENSP00000356842.3; NM_005814.3; NP_005805.1.
DR   UCSC; uc001gea.2; human.
DR   AGR; HGNC:4445; -.
DR   CTD; 10223; -.
DR   DisGeNET; 10223; -.
DR   GeneCards; GPA33; -.
DR   HGNC; HGNC:4445; GPA33.
DR   HPA; ENSG00000143167; Tissue enriched (intestine).
DR   MIM; 602171; gene.
DR   neXtProt; NX_Q99795; -.
DR   OpenTargets; ENSG00000143167; -.
DR   PharmGKB; PA28826; -.
DR   VEuPathDB; HostDB:ENSG00000143167; -.
DR   eggNOG; ENOG502QR0Y; Eukaryota.
DR   GeneTree; ENSGT00940000160248; -.
DR   HOGENOM; CLU_040549_2_0_1; -.
DR   InParanoid; Q99795; -.
DR   OMA; TEMSGYY; -.
DR   OrthoDB; 3024056at2759; -.
DR   PhylomeDB; Q99795; -.
DR   TreeFam; TF330875; -.
DR   PathwayCommons; Q99795; -.
DR   SignaLink; Q99795; -.
DR   BioGRID-ORCS; 10223; 7 hits in 1137 CRISPR screens.
DR   ChiTaRS; GPA33; human.
DR   GeneWiki; GPA33; -.
DR   GenomeRNAi; 10223; -.
DR   Pharos; Q99795; Tbio.
DR   PRO; PR:Q99795; -.
DR   Proteomes; UP000005640; Chromosome 1.
DR   RNAct; Q99795; Protein.
DR   Bgee; ENSG00000143167; Expressed in ileal mucosa and 82 other cell types or tissues.
DR   ExpressionAtlas; Q99795; baseline and differential.
DR   GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0038023; F:signaling receptor activity; TAS:ProtInc.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 2.
DR   InterPro; IPR042474; A33.
DR   InterPro; IPR007110; Ig-like_dom.
DR   InterPro; IPR036179; Ig-like_dom_sf.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR003599; Ig_sub.
DR   InterPro; IPR003598; Ig_sub2.
DR   InterPro; IPR013106; Ig_V-set.
DR   PANTHER; PTHR44969; CELL SURFACE A33 ANTIGEN; 1.
DR   PANTHER; PTHR44969:SF1; CELL SURFACE A33 ANTIGEN; 1.
DR   Pfam; PF13927; Ig_3; 1.
DR   Pfam; PF07686; V-set; 1.
DR   SMART; SM00409; IG; 2.
DR   SMART; SM00408; IGc2; 2.
DR   SMART; SM00406; IGv; 1.
DR   SUPFAM; SSF48726; Immunoglobulin; 2.
DR   PROSITE; PS50835; IG_LIKE; 2.
DR   Genevisible; Q99795; HS.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Glycoprotein;
KW   Immunoglobulin domain; Lipoprotein; Membrane; Palmitate;
KW   Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT   SIGNAL          1..21
FT   CHAIN           22..319
FT                   /note="Cell surface A33 antigen"
FT                   /id="PRO_0000014770"
FT   TOPO_DOM        22..235
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        236..256
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        257..319
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          22..134
FT                   /note="Ig-like V-type"
FT   DOMAIN          140..227
FT                   /note="Ig-like C2-type"
FT   REGION          267..319
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        112
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000269|PubMed:9245713"
FT   CARBOHYD        200
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        223
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        43..117
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT   DISULFID        146..222
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT   DISULFID        162..211
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT   VARIANT         20
FT                   /note="D -> N (in dbSNP:rs2274531)"
FT                   /id="VAR_020079"
FT   VARIANT         165
FT                   /note="K -> N (in dbSNP:rs2228399)"
FT                   /id="VAR_049874"
SQ   SEQUENCE   319 AA;  35632 MW;  9BFC7AAF45C2408E CRC64;
     MVGKMWPVLW TLCAVRVTVD AISVETPQDV LRASQGKSVT LPCTYHTSTS SREGLIQWDK
     LLLTHTERVV IWPFSNKNYI HGELYKNRVS ISNNAEQSDA SITIDQLTMA DNGTYECSVS
     LMSDLEGNTK SRVRLLVLVP PSKPECGIEG ETIIGNNIQL TCQSKEGSPT PQYSWKRYNI
     LNQEQPLAQP ASGQPVSLKN ISTDTSGYYI CTSSNEEGTQ FCNITVAVRS PSMNVALYVG
     IAVGVVAALI IIGIIIYCCC CRGKDDNTED KEDARPNREA YEEPPEQLRE LSREREEEDD
     YRQEEQRSTG RESPDHLDQ
//