Q99795 (GPA33_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cell surface A33 antigen

Alternative name(s):
Glycoprotein A33

Gene names

Name:

GPA33

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in cell-cell recognition and signaling.
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Expressed in normal gastrointestinal epithelium and in 95% of colon cancers.
Post-translational modification	N-glycosylated, contains approximately 8 kDa of N-linked carbohydrate. Palmitoylated.
Sequence similarities	Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Immunoglobulin domain Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc
Molecular_function	receptor activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21
	Chain	22 – 319	298	Cell surface A33 antigen		PRO_0000014770
Regions
	Topological domain	22 – 235	214	Extracellular Potential
	Transmembrane	236 – 256	21	Helical; Potential
	Topological domain	257 – 319	63	Cytoplasmic Potential
	Domain	22 – 134	113	Ig-like V-type
	Domain	140 – 227	88	Ig-like C2-type
	Compositional bias	258 – 261	4	Poly-Cys
Amino acid modifications
	Glycosylation	112	1	N-linked (GlcNAc...)
	Glycosylation	200	1	N-linked (GlcNAc...) Potential
	Glycosylation	223	1	N-linked (GlcNAc...) Potential
	Disulfide bond	43 ↔ 117		Potential
	Disulfide bond	146 ↔ 222		Potential
	Disulfide bond	162 ↔ 211		Potential
Natural variations
	Natural variant	20	1	D → N. Corresponds to variant rs2274531 [ dbSNP | Ensembl ].		VAR_020079
	Natural variant	165	1	K → N. Corresponds to variant rs16858376 [ dbSNP | Ensembl ].		VAR_049874

Sequences

	Sequence		Length	Mass (Da)
	Q99795 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 9BFC7AAF45C2408E	FASTA	319	35,632
	10 20 30 40 50 60 MVGKMWPVLW TLCAVRVTVD AISVETPQDV LRASQGKSVT LPCTYHTSTS SREGLIQWDK 70 80 90 100 110 120 LLLTHTERVV IWPFSNKNYI HGELYKNRVS ISNNAEQSDA SITIDQLTMA DNGTYECSVS 130 140 150 160 170 180 LMSDLEGNTK SRVRLLVLVP PSKPECGIEG ETIIGNNIQL TCQSKEGSPT PQYSWKRYNI 190 200 210 220 230 240 LNQEQPLAQP ASGQPVSLKN ISTDTSGYYI CTSSNEEGTQ FCNITVAVRS PSMNVALYVG 250 260 270 280 290 300 IAVGVVAALI IIGIIIYCCC CRGKDDNTED KEDARPNREA YEEPPEQLRE LSREREEEDD 310 YRQEEQRSTG RESPDHLDQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The human A33 antigen is a transmembrane glycoprotein and a novel member of the immunoglobulin superfamily." Heath J.K., White S.J., Johnstone C.N., Catimel B., Simpson R.J., Moritz R.L., Tu G.-F., Ji H., Whitehead R.H., Groenen L.C., Scott A.M., Ritter G., Cohen L., Welt S., Old L.J., Nice E.C., Burgess A.W. Proc. Natl. Acad. Sci. U.S.A. 94:469-474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Colon carcinoma.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Thymus.
[3]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[6]	"Characterization of posttranslational modifications of human A33 antigen, a novel palmitoylated surface glycoprotein of human gastrointestinal epithelium." Ritter G., Cohen L.S., Nice E.C., Catimel B., Burgess A.W., Moritz R.L., Ji H., Heath J.K., White S.J., Welt S., Old L.J., Simpson R.J. Biochem. Biophys. Res. Commun. 236:682-686(1997) [PubMed] [Europe PMC] [Abstract] Cited for: POST-TRANSLATIONAL MODIFICATIONS.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases
EMBL GenBank DDBJ	U79725 mRNA. Translation: AAC50957.1. AK312833 mRNA. Translation: BAG35687.1. AL158837 Genomic DNA. Translation: CAH69957.1. CH471067 Genomic DNA. Translation: EAW90783.1. BC069705 mRNA. Translation: AAH69705.1. BC069723 mRNA. Translation: AAH69723.1. BC069745 mRNA. Translation: AAH69745.1. BC069761 mRNA. Translation: AAH69761.1. BC069789 mRNA. Translation: AAH69789.1. BC074830 mRNA. Translation: AAH74830.1. BC074876 mRNA. Translation: AAH74876.1. BC107164 mRNA. Translation: AAI07165.1. BC107165 mRNA. Translation: AAI07166.1.
IPI	IPI00293853.
RefSeq	NP_005805.1. NM_005814.1.
UniGene	Hs.651244.
3D structure databases
ProteinModelPortal	Q99795.
ModBase	Search...
PTM databases
PhosphoSite	Q99795.
Polymorphism databases
DMDM	2842765.
Proteomic databases
PaxDb	Q99795.
PeptideAtlas	Q99795.
PRIDE	Q99795.
Protocols and materials databases
DNASU	10223.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000367868; ENSP00000356842; ENSG00000143167.
GeneID	10223.
KEGG	hsa:10223.
UCSC	uc001gea.1. human.
Organism-specific databases
CTD	10223.
GeneCards	GC01M167022.
HGNC	HGNC:4445. GPA33.
HPA	CAB025943. HPA018858.
MIM	602171. gene.
neXtProt	NX_Q99795.
PharmGKB	PA28826.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG44728.
HOGENOM	HOG000112779.
HOVERGEN	HBG051792.
InParanoid	Q99795.
KO	K06790.
OMA	SCNITVA.
OrthoDB	EOG434W6T.
PhylomeDB	Q99795.
Gene expression databases
ArrayExpress	Q99795.
Bgee	Q99795.
CleanEx	HS_GPA33.
Genevestigator	Q99795.
GermOnline	ENSG00000143167. Homo sapiens.
Family and domain databases
Gene3D	2.60.40.10. 2 hits.
InterPro	IPR007110. Ig-like_dom. IPR013783. Ig-like_fold. IPR003598. Ig_sub2. IPR013106. Ig_V-set. IPR003596. Ig_V-set_subgr. [Graphical view]
Pfam	PF07686. V-set. 1 hit. [Graphical view]
SMART	SM00408. IGc2. 1 hit. SM00406. IGv. 1 hit. [Graphical view]
PROSITE	PS50835. IG_LIKE. 2 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	10223.
NextBio	38708.
SOURCE	Search...

Entry information

Entry name

GPA33_HUMAN

Accession

Primary (citable) accession number: Q99795
Secondary accession number(s): Q5VZP6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 1, 1997
Last modified:	May 1, 2013
This is version 118 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.