Q99766 (ATP5S_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP synthase subunit s, mitochondrial

Alternative name(s):
ATP synthase-coupling factor B
Short name=FB
Mitochondrial ATP synthase regulatory component factor B

Gene names

Name:	ATP5S
Synonyms:	ATPW

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	215 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in regulation of mitochondrial membrane ATP synthase. Necessary for H+ conduction of ATP synthase. Facilitates energy-driven catalysis of ATP synthesis by blocking a proton leak through an alternative proton exit pathway By similarity.
Subunit structure	Homotetramer. Associates with ATP synthase By similarity. Ref.2
Subcellular location	Mitochondrion By similarity. Mitochondrion inner membrane By similarity.
Sequence similarities	Belongs to the ATP synthase subunit s family. Contains 4 LRR (leucine-rich) repeats.
Caution	It is uncertain whether Met-1 or Met-16 is the initiator.

Ontologies

Keywords
Biological process	ATP synthesis Hydrogen ion transport Ion transport Transport
Cellular component	CF(0) Membrane Mitochondrion Mitochondrion inner membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Leucine-rich repeat Repeat Transit peptide
Ligand	Magnesium Metal-binding
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	ATP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell proton-transporting ATP synthase complex, coupling factor F(o) Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	hydrogen ion transmembrane transporter activity Non-traceable author statement PubMed 6143319. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q99766-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q99766-2)

The sequence of this isoform differs from the canonical sequence as follows:
     121-136: EGLEHVEKIRLCKCHY → GNYPIVLLIENADDLQ
     137-215: Missing.

Isoform 3 (identifier: Q99766-3)

The sequence of this isoform differs from the canonical sequence as follows:
     122-127: GLEHVE → TSNICC
     128-215: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 40	40	Mitochondrion
	Chain	41 – 215	175	ATP synthase subunit s, mitochondrial		PRO_0000002538
Regions
	Repeat	77 – 102	26	LRR 1
	Repeat	103 – 131	29	LRR 2
	Repeat	132 – 156	25	LRR 3
	Repeat	157 – 188	32	LRR 4
	Region	1 – 76	76	N-terminal domain
Sites
	Metal binding	74	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	108	1	Magnesium By similarity
Natural variations
	Alternative sequence	121 – 136	16	EGLEH…CKCHY → GNYPIVLLIENADDLQ in isoform 2.		VSP_040059
	Alternative sequence	122 – 127	6	GLEHVE → TSNICC in isoform 3.		VSP_040060
	Alternative sequence	128 – 215	88	Missing in isoform 3.		VSP_040061
	Alternative sequence	137 – 215	79	Missing in isoform 2.		VSP_040062
	Natural variant	18	1	P → L. Ref.1 Ref.2 Ref.3 Ref.5 Ref.6 Corresponds to variant rs2275592 [ dbSNP | Ensembl ].		VAR_060296

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 30, 2010. Version 3. Checksum: DFDCDE6D81E5DC62	FASTA	215	24,866
	10 20 30 40 50 60 MCCAVSEQRL TCADQMMPFG KISQQLCGVK KLPWSCDSRY FWGWLNAVFN KVDYDRIRDV 70 80 90 100 110 120 GPDRAASEWL LRCGAMVRYH GQERWQKDYN HLPTGPLDKY KIQAIDATDS CIMSIGFDHM 130 140 150 160 170 180 EGLEHVEKIR LCKCHYIEDD CLLRLSQLEN LQKTILEMEI ISCGNITDKG IIALRHLRNL 190 200 210 KYLLLSDLPG VREKENLVQA FKTALPSLEL KLQLK
	Isoform 2 [UniParc]. Checksum: C69A8CD2A7720FCC	FASTA	136	15,685
	10 20 30 40 50 60 MCCAVSEQRL TCADQMMPFG KISQQLCGVK KLPWSCDSRY FWGWLNAVFN KVDYDRIRDV 70 80 90 100 110 120 GPDRAASEWL LRCGAMVRYH GQERWQKDYN HLPTGPLDKY KIQAIDATDS CIMSIGFDHM 130 GNYPIVLLIE NADDLQ
	Isoform 3 [UniParc]. Checksum: 7C0681D95BD6EABB	FASTA	127	14,667
	10 20 30 40 50 60 MCCAVSEQRL TCADQMMPFG KISQQLCGVK KLPWSCDSRY FWGWLNAVFN KVDYDRIRDV 70 80 90 100 110 120 GPDRAASEWL LRCGAMVRYH GQERWQKDYN HLPTGPLDKY KIQAIDATDS CIMSIGFDHM ETSNICC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Large-scale concatenation cDNA sequencing." Yu W., Andersson B., Worley K.C., Muzny D.M., Ding Y., Liu W., Ricafrente J.Y., Wentland M.A., Lennon G., Gibbs R.A. Genome Res. 7:353-358(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT LEU-18. Tissue: Brain.
[2]	"Factor B and the mitochondrial ATP synthase complex." Belogrudov G.I., Hatefi Y. J. Biol. Chem. 277:6097-6103(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PARTIAL PROTEIN SEQUENCE, SUBUNIT, VARIANT LEU-18.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT LEU-18. Tissue: Hippocampus.
[4]	"The DNA sequence and analysis of human chromosome 14." Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J. Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT LEU-18.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), VARIANT LEU-18. Tissue: Uterus.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U79253 mRNA. Translation: AAB50202.1. AY052377 mRNA. Translation: AAL13058.1. AK290008 mRNA. Translation: BAF82697.1. AL359397 Genomic DNA. No translation available. CH471078 Genomic DNA. Translation: EAW65718.1. CH471078 Genomic DNA. Translation: EAW65722.1. BC011549 mRNA. Translation: AAH11549.1.
IPI	IPI00017816. IPI00257300. IPI00455157.
RefSeq	NP_001003803.1. NM_001003803.2. NP_001003805.1. NM_001003805.2. NP_056499.2. NM_015684.3.
UniGene	Hs.438489.
3D structure databases
ProteinModelPortal	Q99766.
SMR	Q99766. Positions 41-215.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q99766. 1 interaction.
STRING	9606.ENSP00000308334.
PTM databases
PhosphoSite	Q99766.
Polymorphism databases
DMDM	20532387.
Proteomic databases
PaxDb	Q99766.
PRIDE	Q99766.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000245448; ENSP00000245448; ENSG00000125375. ENST00000311459; ENSP00000308334; ENSG00000125375. ENST00000426751; ENSP00000389246; ENSG00000125375.
GeneID	27109.
KEGG	hsa:27109.
UCSC	uc001wxv.3. human. uc001wxw.2. human. uc001wxx.2. human.
Organism-specific databases
CTD	27109.
GeneCards	GC14P050782.
HGNC	HGNC:18799. ATP5S.
HPA	HPA046967.
neXtProt	NX_Q99766.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG307162.
HOGENOM	HOG000230741.
HOVERGEN	HBG050615.
KO	K07554.
OMA	TESCIMY.
OrthoDB	EOG4SXNDG.
Gene expression databases
ArrayExpress	Q99766.
Bgee	Q99766.
CleanEx	HS_ATP5S.
Genevestigator	Q99766.
GermOnline	ENSG00000125375. Homo sapiens.
Family and domain databases
InterPro	IPR026063. ATP_synthase_s. [Graphical view]
PANTHER	PTHR13382. PTHR13382. 1 hit.
PROSITE	PS51450. LRR. False negative. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	ATP5S. human.
GenomeRNAi	27109.
NextBio	49778.

Entry information

Entry name

ATP5S_HUMAN

Accession

Primary (citable) accession number: Q99766
Secondary accession number(s): A8K1U3 , D9N156, Q8WWX3, Q96F77

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 30, 2010
Last modified:	April 3, 2013
This is version 101 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.