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Protein

MyoD family inhibitor

Gene

MDFI

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits the transactivation activity of the Myod family of myogenic factors and represses myogenesis. Acts by associating with Myod family members and retaining them in the cytoplasm by masking their nuclear localization signals. Can also interfere with the DNA-binding activity of Myod family members. Plays an important role in trophoblast and chondrogenic differentiation. Regulates the transcriptional activity of TCF7L1/TCF3 by interacting directly with TCF7L1/TCF3 and preventing it from binding DNA. Binds to the axin complex, resulting in an increase in the level of free beta-catenin. Affects axin regulation of the WNT and JNK signaling pathways (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation

Names & Taxonomyi

Protein namesi
Recommended name:
MyoD family inhibitor
Alternative name(s):
Myogenic repressor I-mf
Gene namesi
Name:MDFI
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:6967. MDFI.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: ProtInc
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA30713.

Polymorphism and mutation databases

BioMutaiMDFI.
DMDMi25090674.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 246246MyoD family inhibitorPRO_0000096324Add
BLAST

Proteomic databases

MaxQBiQ99750.
PaxDbiQ99750.
PRIDEiQ99750.

PTM databases

PhosphoSiteiQ99750.

Expressioni

Gene expression databases

BgeeiQ99750.
CleanExiHS_MDFI.
ExpressionAtlasiQ99750. baseline and differential.
GenevisibleiQ99750. HS.

Organism-specific databases

HPAiHPA049973.

Interactioni

Subunit structurei

The C-terminus interacts with AXIN1 and LEF1.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Q5BKY63EBI-724076,EBI-10243533
Q5W1503EBI-724076,EBI-10248148
Q5XG853EBI-724076,EBI-10248413
AANATQ166133EBI-724076,EBI-7451846
ADAMTSL3P829873EBI-724076,EBI-10221726
AKAP17AQ02040-33EBI-724076,EBI-10222656
AQP1P299724EBI-724076,EBI-745213
AQP5P550643EBI-724076,EBI-746103
ARHGAP32A7KAX93EBI-724076,EBI-308663
ARID5AC9J1Q03EBI-724076,EBI-10176351
AVPI1Q5T6864EBI-724076,EBI-8640233
BAHD1Q8TBE04EBI-724076,EBI-742750
BBS2Q9BXC93EBI-724076,EBI-748297
BEX1Q9HBH73EBI-724076,EBI-7162175
C10orf62Q5T6817EBI-724076,EBI-744052
C14orf105Q17R993EBI-724076,EBI-10238351
C19orf60Q96EN93EBI-724076,EBI-745810
C19orf66Q9NUL53EBI-724076,EBI-10313866
C20orf195Q9BVV23EBI-724076,EBI-744935
C22orf39Q6P5X53EBI-724076,EBI-7317823
C4orf26Q17RF53EBI-724076,EBI-10239029
C8orf48Q96LL43EBI-724076,EBI-751596
CBFA2T2O434394EBI-724076,EBI-748628
CBX2Q147813EBI-724076,EBI-745934
CC2D1BQ5T0F93EBI-724076,EBI-10245204
CCDC116Q8IYX34EBI-724076,EBI-744311
CDCA7LQ96GN53EBI-724076,EBI-5278764
CDKL3Q8IVW43EBI-724076,EBI-3919850
CEP57L1Q8IYX83EBI-724076,EBI-1104570
CNNM3Q8NE013EBI-724076,EBI-741032
CREB5Q02930-33EBI-724076,EBI-10192698
DAAM2Q6DCB03EBI-724076,EBI-10249414
DCAF8Q5TAQ96EBI-724076,EBI-740686
DCANP1Q8TF633EBI-724076,EBI-10275670
DKK1O949073EBI-724076,EBI-742864
DMRT3Q9NQL93EBI-724076,EBI-9679045
DOCK2Q926083EBI-724076,EBI-448771
EIF4A2Q14240-23EBI-724076,EBI-10232522
ENKD1Q9H0I25EBI-724076,EBI-744099
FAAP100Q0VG06-33EBI-724076,EBI-10226932
FAM124BQ9H5Z64EBI-724076,EBI-741626
FAM161AQ3B8203EBI-724076,EBI-719941
FAM27E3Q08E933EBI-724076,EBI-2602739
FBXL18Q96D165EBI-724076,EBI-744419
FBXO34Q9NWN33EBI-724076,EBI-719816
FOXD4L6Q3SYB33EBI-724076,EBI-6425864
GATA2P237693EBI-724076,EBI-2806671
GNAI2P048995EBI-724076,EBI-353997
GNG5P632183EBI-724076,EBI-10220734
GPATCH2LQ9NWQ43EBI-724076,EBI-5666657
GTF3C5Q9Y5Q83EBI-724076,EBI-1045409
hCG_20425B4DNA43EBI-724076,EBI-7399002
HEYLQ9NQ874EBI-724076,EBI-751092
HNRNPLLQ8WVV94EBI-724076,EBI-535849
Hoxa1P090223EBI-724076,EBI-3957603From a different organism.
HOXB9P174823EBI-724076,EBI-745290
HTR1BP282223EBI-724076,EBI-1056863
KAT5Q929933EBI-724076,EBI-399080
KIAA0040Q150533EBI-724076,EBI-2805673
KPNA2Q6NVW73EBI-724076,EBI-9377406
LASP1Q148473EBI-724076,EBI-742828
LCE1BQ5T7P33EBI-724076,EBI-10245913
LCE3EQ5T5B03EBI-724076,EBI-10245456
LCE4AQ5TA783EBI-724076,EBI-10246358
LIMS2Q7Z4I7-53EBI-724076,EBI-10257651
LINC01588A1A4T83EBI-724076,EBI-10171684
LRCH4O754273EBI-724076,EBI-718707
MAGED1Q9Y5V33EBI-724076,EBI-716006
MAGEF1Q9HAY23EBI-724076,EBI-5525855
MAPKBP1O603363EBI-724076,EBI-947402
NEU4Q8WWR84EBI-724076,EBI-746964
NPDC1Q9NQX55EBI-724076,EBI-748927
NR1H2F1D8P73EBI-724076,EBI-10177172
NR1H2P550553EBI-724076,EBI-745354
NR1H3Q131333EBI-724076,EBI-781356
OLIG3Q7RTU33EBI-724076,EBI-10225049
PARP11F8WBZ73EBI-724076,EBI-10177820
PDIA5Q145543EBI-724076,EBI-953879
PHLDA1Q8WV243EBI-724076,EBI-738731
PID1Q7Z2X43EBI-724076,EBI-10256685
PIDD1Q9HB753EBI-724076,EBI-520427
PIH1D2Q8WWB53EBI-724076,EBI-10232538
PIN1Q135264EBI-724076,EBI-714158
PLEKHG4Q58EX73EBI-724076,EBI-949255
PRKAB2O437413EBI-724076,EBI-1053424
PTPMT1Q8WUK04EBI-724076,EBI-7199479
PVRL2Q926924EBI-724076,EBI-718419
RABL6Q3YEC72EBI-724076,EBI-742029
REG3AQ061413EBI-724076,EBI-10223932
RIPPLY1Q0D2K33EBI-724076,EBI-10226430
RTP5Q14D333EBI-724076,EBI-10217913
SIX1Q154752EBI-724076,EBI-743675
SLC25A10Q9UBX37EBI-724076,EBI-750394
SPATA8Q6RVD63EBI-724076,EBI-8635958
SPG7Q9UQ904EBI-724076,EBI-717201
SPRY1O436093EBI-724076,EBI-3866665
SPRY2O435973EBI-724076,EBI-742487
TCEB3Q142413EBI-724076,EBI-742350
TGIF1Q155834EBI-724076,EBI-714215
THEGQ9P2T03EBI-724076,EBI-751020
TNNI1P192373EBI-724076,EBI-746692
TNP2Q4VB563EBI-724076,EBI-10241829
TRPV6Q9Y2604EBI-724076,EBI-750052
TTC23Q5W5X93EBI-724076,EBI-6447954
TTLL10Q6ZVT03EBI-724076,EBI-7844656
TUSC2O758963EBI-724076,EBI-1052725
TYMSOSQ8TAI14EBI-724076,EBI-742060
TYRO3Q064183EBI-724076,EBI-3951628
USP20Q9Y2K63EBI-724076,EBI-2511991
VHLP403373EBI-724076,EBI-301246
VPS72Q159064EBI-724076,EBI-399189
WNT11O960143EBI-724076,EBI-8058160
ZBTB24O431676EBI-724076,EBI-744471
ZBTB25P242782EBI-724076,EBI-739899
ZBTB9Q96C003EBI-724076,EBI-395708
ZFYVE26Q96H433EBI-724076,EBI-10286915
ZNF101Q8IZC73EBI-724076,EBI-5278328
ZNF124Q159733EBI-724076,EBI-2555767
ZNF136P527377EBI-724076,EBI-749129
ZNF138P52744-23EBI-724076,EBI-10213071
ZNF155Q12901-23EBI-724076,EBI-10227379
ZNF223Q9UK113EBI-724076,EBI-10322867
ZNF230Q9UIE03EBI-724076,EBI-1105361
ZNF264Q14C613EBI-724076,EBI-2826570
ZNF266Q145843EBI-724076,EBI-7115319
ZNF408Q9H9D43EBI-724076,EBI-347633
ZNF417Q8TAU34EBI-724076,EBI-740727
ZNF433Q8N7K03EBI-724076,EBI-10267553
ZNF439Q8NDP45EBI-724076,EBI-747580
ZNF440Q8IYI85EBI-724076,EBI-726439
ZNF490Q9ULM24EBI-724076,EBI-1105370
ZNF559Q9BR844EBI-724076,EBI-746605
ZNF564Q8TBZ83EBI-724076,EBI-10273713
ZNF578Q3MI943EBI-724076,EBI-10241108
ZNF581Q9P0T44EBI-724076,EBI-745520
ZNF587Q96SQ53EBI-724076,EBI-6427977
ZNF607Q96SK34EBI-724076,EBI-747175
ZNF646O150153EBI-724076,EBI-745608
ZNF79Q159373EBI-724076,EBI-10237274
ZNF844Q08AG53EBI-724076,EBI-10225757

Protein-protein interaction databases

BioGridi110353. 225 interactions.
IntActiQ99750. 219 interactions.
MINTiMINT-190174.
STRINGi9606.ENSP00000230321.

Structurei

3D structure databases

ProteinModelPortaliQ99750.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi163 – 24381Cys-richAdd
BLAST

Sequence similaritiesi

Belongs to the MDFI family.Curated

Phylogenomic databases

eggNOGiNOG43212.
GeneTreeiENSGT00390000010600.
HOGENOMiHOG000001577.
HOVERGENiHBG031704.
InParanoidiQ99750.
OMAiQAQEDCC.
PhylomeDBiQ99750.
TreeFamiTF332113.

Family and domain databases

InterProiIPR026134. MDFI/MDFIC.
[Graphical view]
PANTHERiPTHR15304. PTHR15304. 1 hit.
PfamiPF15316. MDFI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99750-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYQVSGQRPS GCDAPYGAPS AAPGPAQTLS LLPGLEVVTG STHPAEAAPE
60 70 80 90 100
EGSLEEAATP MPQGNGPGIP QGLDSTDLDV PTEAVTCQPQ GNPLGCTPLL
110 120 130 140 150
PNDSGHPSEL GGTRRAGNGA LGGPKAHRKL QTHPSLASQG SKKSKSSSKS
160 170 180 190 200
TTSQIPLQAQ EDCCVHCILS CLFCEFLTLC NIVLDCATCG SCSSEDSCLC
210 220 230 240
CCCCGSGECA DCDLPCDLDC GILDACCESA DCLEICMECC GLCFSS
Length:246
Mass (Da):25,029
Last modified:May 1, 1997 - v1
Checksum:i9330E52141B79E5A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U78313 mRNA. Translation: AAB39748.1.
AL035588 Genomic DNA. Translation: CAB54148.1.
BC007836 mRNA. Translation: AAH07836.1.
CCDSiCCDS4857.1.
RefSeqiNP_001287733.1. NM_001300804.1.
NP_001287734.1. NM_001300805.1.
NP_001287735.1. NM_001300806.1.
NP_005577.1. NM_005586.3.
UniGeneiHs.520119.

Genome annotation databases

EnsembliENST00000230321; ENSP00000230321; ENSG00000112559.
ENST00000373051; ENSP00000362142; ENSG00000112559.
GeneIDi4188.
KEGGihsa:4188.
UCSCiuc003oqq.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U78313 mRNA. Translation: AAB39748.1.
AL035588 Genomic DNA. Translation: CAB54148.1.
BC007836 mRNA. Translation: AAH07836.1.
CCDSiCCDS4857.1.
RefSeqiNP_001287733.1. NM_001300804.1.
NP_001287734.1. NM_001300805.1.
NP_001287735.1. NM_001300806.1.
NP_005577.1. NM_005586.3.
UniGeneiHs.520119.

3D structure databases

ProteinModelPortaliQ99750.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110353. 225 interactions.
IntActiQ99750. 219 interactions.
MINTiMINT-190174.
STRINGi9606.ENSP00000230321.

PTM databases

PhosphoSiteiQ99750.

Polymorphism and mutation databases

BioMutaiMDFI.
DMDMi25090674.

Proteomic databases

MaxQBiQ99750.
PaxDbiQ99750.
PRIDEiQ99750.

Protocols and materials databases

DNASUi4188.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000230321; ENSP00000230321; ENSG00000112559.
ENST00000373051; ENSP00000362142; ENSG00000112559.
GeneIDi4188.
KEGGihsa:4188.
UCSCiuc003oqq.4. human.

Organism-specific databases

CTDi4188.
GeneCardsiGC06P041604.
HGNCiHGNC:6967. MDFI.
HPAiHPA049973.
MIMi604971. gene.
neXtProtiNX_Q99750.
PharmGKBiPA30713.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG43212.
GeneTreeiENSGT00390000010600.
HOGENOMiHOG000001577.
HOVERGENiHBG031704.
InParanoidiQ99750.
OMAiQAQEDCC.
PhylomeDBiQ99750.
TreeFamiTF332113.

Miscellaneous databases

ChiTaRSiMDFI. human.
GeneWikiiMDFI.
GenomeRNAii4188.
NextBioi16502.
PROiQ99750.
SOURCEiSearch...

Gene expression databases

BgeeiQ99750.
CleanExiHS_MDFI.
ExpressionAtlasiQ99750. baseline and differential.
GenevisibleiQ99750. HS.

Family and domain databases

InterProiIPR026134. MDFI/MDFIC.
[Graphical view]
PANTHERiPTHR15304. PTHR15304. 1 hit.
PfamiPF15316. MDFI. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The gene encoding I-mf (Mdfi) maps to human chromosome 6p21 and mouse chromosome 17."
    Kraut N.
    Mamm. Genome 8:618-619(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Fetal brain.
  2. "The DNA sequence and analysis of human chromosome 6."
    Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
    Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Muscle.

Entry informationi

Entry nameiMDFI_HUMAN
AccessioniPrimary (citable) accession number: Q99750
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: May 1, 1997
Last modified: June 24, 2015
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.