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Protein

Gamma-soluble NSF attachment protein

Gene

NAPG

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus.

GO - Molecular functioni

GO - Biological processi

  • intracellular protein transport Source: GO_Central
  • intra-Golgi vesicle-mediated transport Source: ProtInc
  • membrane fusion Source: ProtInc
  • protein complex assembly Source: UniProtKB
  • protein stabilization Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-HSA-6807878. COPI-mediated anterograde transport.
R-HSA-6811434. COPI-dependent Golgi-to-ER retrograde traffic.
R-HSA-6811438. Intra-Golgi traffic.
R-HSA-6811440. Retrograde transport at the Trans-Golgi-Network.

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-soluble NSF attachment protein
Short name:
SNAP-gamma
Alternative name(s):
N-ethylmaleimide-sensitive factor attachment protein gamma
Gene namesi
Name:NAPG
Synonyms:SNAPG
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 18

Organism-specific databases

HGNCiHGNC:7642. NAPG.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
  • lysosomal membrane Source: UniProtKB
  • mitochondrion Source: UniProtKB
  • myelin sheath Source: Ensembl
  • SNARE complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA31445.

Polymorphism and mutation databases

BioMutaiNAPG.
DMDMi3024632.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 312312Gamma-soluble NSF attachment proteinPRO_0000219063Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei284 – 2841PhosphoserineBy similarity
Modified residuei287 – 2871PhosphothreonineBy similarity
Modified residuei308 – 3081PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ99747.
MaxQBiQ99747.
PaxDbiQ99747.
PRIDEiQ99747.

2D gel databases

OGPiQ99747.

PTM databases

iPTMnetiQ99747.
PhosphoSiteiQ99747.
SwissPalmiQ99747.

Expressioni

Gene expression databases

BgeeiQ99747.
CleanExiHS_NAPG.
ExpressionAtlasiQ99747. baseline and differential.
GenevisibleiQ99747. HS.

Organism-specific databases

HPAiHPA008208.
HPA011389.

Interactioni

Subunit structurei

Binds RIP11. Interacts with VTI1A (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi114304. 32 interactions.
IntActiQ99747. 4 interactions.
STRINGi9606.ENSP00000324628.

Structurei

3D structure databases

ProteinModelPortaliQ99747.
SMRiQ99747. Positions 2-276.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SNAP family.Curated

Phylogenomic databases

eggNOGiKOG1585. Eukaryota.
ENOG410ZYD7. LUCA.
GeneTreeiENSGT00390000010109.
HOGENOMiHOG000007747.
HOVERGENiHBG001648.
InParanoidiQ99747.
OMAiAVCFKNA.
OrthoDBiEOG7FFMS2.
PhylomeDBiQ99747.
TreeFamiTF312872.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR000744. NSF_attach.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PANTHERiPTHR13768. PTHR13768. 2 hits.
SUPFAMiSSF48452. SSF48452. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q99747-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAQKINEGL EHLAKAEKYL KTGFLKWKPD YDSAASEYGK AAVAFKNAKQ
60 70 80 90 100
FEQAKDACLR EAVAHENNRA LFHAAKAYEQ AGMMLKEMQK LPEAVQLIEK
110 120 130 140 150
ASMMYLENGT PDTAAMALER AGKLIENVDP EKAVQLYQQT ANVFENEERL
160 170 180 190 200
RQAVELLGKA SRLLVRGRRF DEAALSIQKE KNIYKEIENY PTCYKKTIAQ
210 220 230 240 250
VLVHLHRNDY VAAERCVRES YSIPGFNGSE DCAALEQLLE GYDQQDQDQV
260 270 280 290 300
SDVCNSPLFK YMDNDYAKLG LSLVVPGGGI KKKSPATPQA KPDGVTATAA
310
DEEEDEYSGG LC
Length:312
Mass (Da):34,746
Last modified:May 1, 1997 - v1
Checksum:iC37D73C1AEFD48C8
GO
Isoform 2 (identifier: Q99747-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-82: Missing.

Note: No experimental confirmation available.
Show »
Length:230
Mass (Da):25,662
Checksum:i1243534338B3B8D2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti147 – 1471E → D in AAH01889 (PubMed:15489334).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti92 – 921P → S.
Corresponds to variant rs16974765 [ dbSNP | Ensembl ].
VAR_052027
Natural varianti281 – 2811K → N.
Corresponds to variant rs2305370 [ dbSNP | Ensembl ].
VAR_020129

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 8282Missing in isoform 2. 1 PublicationVSP_056355Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U78107 mRNA. Translation: AAB69753.1.
AK294038 mRNA. Translation: BAG57390.1.
AP001099 Genomic DNA. No translation available.
BC001889 mRNA. Translation: AAH01889.1.
CCDSiCCDS45827.1. [Q99747-1]
RefSeqiNP_003817.1. NM_003826.2. [Q99747-1]
XP_011524058.1. XM_011525756.1. [Q99747-2]
UniGeneiHs.464622.

Genome annotation databases

EnsembliENST00000322897; ENSP00000324628; ENSG00000134265. [Q99747-1]
GeneIDi8774.
KEGGihsa:8774.
UCSCiuc002kon.4. human. [Q99747-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U78107 mRNA. Translation: AAB69753.1.
AK294038 mRNA. Translation: BAG57390.1.
AP001099 Genomic DNA. No translation available.
BC001889 mRNA. Translation: AAH01889.1.
CCDSiCCDS45827.1. [Q99747-1]
RefSeqiNP_003817.1. NM_003826.2. [Q99747-1]
XP_011524058.1. XM_011525756.1. [Q99747-2]
UniGeneiHs.464622.

3D structure databases

ProteinModelPortaliQ99747.
SMRiQ99747. Positions 2-276.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114304. 32 interactions.
IntActiQ99747. 4 interactions.
STRINGi9606.ENSP00000324628.

PTM databases

iPTMnetiQ99747.
PhosphoSiteiQ99747.
SwissPalmiQ99747.

Polymorphism and mutation databases

BioMutaiNAPG.
DMDMi3024632.

2D gel databases

OGPiQ99747.

Proteomic databases

EPDiQ99747.
MaxQBiQ99747.
PaxDbiQ99747.
PRIDEiQ99747.

Protocols and materials databases

DNASUi8774.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000322897; ENSP00000324628; ENSG00000134265. [Q99747-1]
GeneIDi8774.
KEGGihsa:8774.
UCSCiuc002kon.4. human. [Q99747-1]

Organism-specific databases

CTDi8774.
GeneCardsiNAPG.
HGNCiHGNC:7642. NAPG.
HPAiHPA008208.
HPA011389.
MIMi603216. gene.
neXtProtiNX_Q99747.
PharmGKBiPA31445.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1585. Eukaryota.
ENOG410ZYD7. LUCA.
GeneTreeiENSGT00390000010109.
HOGENOMiHOG000007747.
HOVERGENiHBG001648.
InParanoidiQ99747.
OMAiAVCFKNA.
OrthoDBiEOG7FFMS2.
PhylomeDBiQ99747.
TreeFamiTF312872.

Enzyme and pathway databases

ReactomeiR-HSA-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-HSA-6807878. COPI-mediated anterograde transport.
R-HSA-6811434. COPI-dependent Golgi-to-ER retrograde traffic.
R-HSA-6811438. Intra-Golgi traffic.
R-HSA-6811440. Retrograde transport at the Trans-Golgi-Network.

Miscellaneous databases

ChiTaRSiNAPG. human.
GeneWikiiNAPG.
GenomeRNAii8774.
PROiQ99747.
SOURCEiSearch...

Gene expression databases

BgeeiQ99747.
CleanExiHS_NAPG.
ExpressionAtlasiQ99747. baseline and differential.
GenevisibleiQ99747. HS.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR000744. NSF_attach.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PANTHERiPTHR13768. PTHR13768. 2 hits.
SUPFAMiSSF48452. SSF48452. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Regulated secretion in platelets: identification of elements of the platelet exocytosis machinery."
    Lemons P.P., Chen D., Bernstein A.M., Bennett M.K., Whiteheart S.W.
    Blood 90:1490-1500(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Cerebellum.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Lung.
  5. "Gaf-1, a gamma-SNAP-binding protein associated with the mitochondria."
    Chen D., Xu W., He P., Medrano E.E., Whiteheart S.W.
    J. Biol. Chem. 276:13127-13135(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RIP11.
  6. "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
    Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
    Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSNAG_HUMAN
AccessioniPrimary (citable) accession number: Q99747
Secondary accession number(s): B4DFC9, Q9BUV1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: May 1, 1997
Last modified: June 8, 2016
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.