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Q99735 (MGST2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Microsomal glutathione S-transferase 2

Short name=Microsomal GST-2
EC=2.5.1.18
Alternative name(s):
Microsomal GST-II
Gene names
Name:MGST2
Synonyms:GST2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length147 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Can catalyze the production of LTC4 from LTA4 and reduced glutathione. Can catalyze the conjugation of 1-chloro-2,4-dinitrobenzene with reduced glutathione.

Catalytic activity

RX + glutathione = HX + R-S-glutathione.

Subunit structure

Homotrimer.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein Potential. Microsome membrane; Multi-pass membrane protein Potential.

Tissue specificity

Liver, spleen, skeletal muscle, heart, adrenals, pancreas, prostate, testis, fetal liver, and fetal spleen. Very low expression in lung, brain, placenta and bone marrow.

Sequence similarities

Belongs to the MAPEG family.

Ontologies

Keywords
   Biological processLeukotriene biosynthesis
   Cellular componentEndoplasmic reticulum
Membrane
Microsome
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainTransmembrane
Transmembrane helix
   Molecular functionTransferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglutathione biosynthetic process

Inferred from direct assay Ref.1. Source: UniProtKB

glutathione derivative biosynthetic process

Traceable author statement. Source: Reactome

leukotriene biosynthetic process

Inferred from direct assay Ref.1PubMed 9092565. Source: UniProtKB

oxidation-reduction process

Inferred from Biological aspect of Ancestor. Source: GOC

response to lipopolysaccharide

Inferred from electronic annotation. Source: Ensembl

response to organonitrogen compound

Inferred from electronic annotation. Source: Ensembl

small molecule metabolic process

Traceable author statement. Source: Reactome

xenobiotic metabolic process

Traceable author statement. Source: Reactome

   Cellular_componentendoplasmic reticulum membrane

Traceable author statement. Source: Reactome

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

intracellular membrane-bounded organelle

Inferred from direct assay Ref.1. Source: UniProtKB

nuclear envelope

Inferred from Biological aspect of Ancestor. Source: RefGenome

plasma membrane

Inferred from direct assay PubMed 9092565. Source: UniProtKB

   Molecular_functionenzyme activator activity

Inferred from electronic annotation. Source: InterPro

glutathione peroxidase activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

glutathione transferase activity

Inferred from direct assay Ref.1. Source: UniProtKB

leukotriene-C4 synthase activity

Inferred from direct assay Ref.1PubMed 9092565. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q99735-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q99735-2)

The sequence of this isoform differs from the canonical sequence as follows:
     53-147: QQNCVEFYPI...NIAKKLRRQF → HFCYLSGSGVHIWPSPILLGIFRSC
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 147147Microsomal glutathione S-transferase 2
PRO_0000217740

Regions

Transmembrane6 – 2621Helical; Potential
Transmembrane59 – 7921Helical; Potential
Transmembrane111 – 13121Helical; Potential

Natural variations

Alternative sequence53 – 14795QQNCV…LRRQF → HFCYLSGSGVHIWPSPILLG IFRSC in isoform 2.
VSP_044538
Natural variant1011A → V. Ref.3
Corresponds to variant rs8192111 [ dbSNP | Ensembl ].
VAR_019997

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: D0E89B46885D16EF

FASTA14716,621
        10         20         30         40         50         60 
MAGNSILLAA VSILSACQQS YFALQVGKAR LKYKVTPPAV TGSPEFERVF RAQQNCVEFY 

        70         80         90        100        110        120 
PIFIITLWMA GWYFNQVFAT CLGLVYIYGR HLYFWGYSEA AKKRITGFRL SLGILALLTL 

       130        140 
LGALGIANSF LDEYLDLNIA KKLRRQF 

« Hide

Isoform 2 [UniParc].

Checksum: CABF1D4ECFAD2B79
Show »

FASTA778,359

References

« Hide 'large scale' references
[1]"Identification and characterization of a novel human microsomal glutathione S-transferase with leukotriene C4 synthase activity and significant sequence identity to 5-lipoxygenase-activating protein and leukotriene C4 synthase."
Jakobsson P.-J., Mancini J.A., Ford-Hutchinson A.W.
J. Biol. Chem. 271:22203-22210(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]NIEHS SNPs program
Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT VAL-101.
[4]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Lung and Neuroblastoma.
[6]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Web resources

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U77604 mRNA. Translation: AAC51768.1.
CR407640 mRNA. Translation: CAG28568.1.
AY341028 Genomic DNA. Translation: AAP88934.1. Sequence problems.
AC108053 Genomic DNA. No translation available.
AC112236 Genomic DNA. No translation available.
BC025416 mRNA. Translation: AAH25416.1.
BG519599 mRNA. No translation available.
CCDSCCDS3749.1. [Q99735-1]
CCDS56339.1. [Q99735-2]
RefSeqNP_001191295.1. NM_001204366.1. [Q99735-1]
NP_001191297.1. NM_001204368.1. [Q99735-2]
NP_002404.1. NM_002413.4. [Q99735-1]
UniGeneHs.81874.

3D structure databases

ProteinModelPortalQ99735.
SMRQ99735. Positions 3-131.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000265498.

Chemistry

ChEMBLCHEMBL1743185.
DrugBankDB00143. Glutathione.

PTM databases

PhosphoSiteQ99735.

Polymorphism databases

DMDM2842764.

Proteomic databases

MaxQBQ99735.
PaxDbQ99735.
PRIDEQ99735.

Protocols and materials databases

DNASU4258.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000265498; ENSP00000265498; ENSG00000085871. [Q99735-1]
ENST00000503816; ENSP00000423008; ENSG00000085871. [Q99735-1]
ENST00000506797; ENSP00000424278; ENSG00000085871. [Q99735-2]
GeneID4258.
KEGGhsa:4258.
UCSCuc003ihy.3. human. [Q99735-1]

Organism-specific databases

CTD4258.
GeneCardsGC04P140586.
HGNCHGNC:7063. MGST2.
HPAHPA010707.
MIM601733. gene.
neXtProtNX_Q99735.
PharmGKBPA30792.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG77136.
HOGENOMHOG000116372.
HOVERGENHBG105513.
InParanoidQ99735.
KOK00799.
OMALYIYARH.
OrthoDBEOG7GXPD5.
PhylomeDBQ99735.
TreeFamTF105328.

Enzyme and pathway databases

ReactomeREACT_111217. Metabolism.

Gene expression databases

BgeeQ99735.
CleanExHS_MGST2.
GenevestigatorQ99735.

Family and domain databases

Gene3D1.20.120.550. 1 hit.
InterProIPR001446. 5_LipOase_AP.
IPR018295. FLAP/GST2/LTC4S_CS.
IPR023352. MAPEG-like_dom.
IPR001129. Membr-assoc_MAPEG.
[Graphical view]
PfamPF01124. MAPEG. 1 hit.
[Graphical view]
PRINTSPR00488. 5LPOXGNASEAP.
PROSITEPS01297. FLAP_GST2_LTC4S. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSMGST2. human.
GeneWikiMGST2.
GenomeRNAi4258.
NextBio16797.
PROQ99735.
SOURCESearch...

Entry information

Entry nameMGST2_HUMAN
AccessionPrimary (citable) accession number: Q99735
Secondary accession number(s): D6RBB5, Q7Z5B8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 1997
Last modified: July 9, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM