Q99735 (MGST2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Microsomal glutathione S-transferase 2
Short name=Microsomal GST-2
EC=2.5.1.18

Alternative name(s):
Microsomal GST-II

Gene names

Name:	MGST2
Synonyms:	GST2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	147 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Can catalyze the production of LTC4 from LTA4 and reduced glutathione. Can catalyze the conjugation of 1-chloro-2,4-dinitrobenzene with reduced glutathione.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subunit structure	Homotrimer.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Potential. Microsome membrane; Multi-pass membrane protein Potential.
Tissue specificity	Liver, spleen, skeletal muscle, heart, adrenals, pancreas, prostate, testis, fetal liver, and fetal spleen. Very low expression in lung, brain, placenta and bone marrow.
Sequence similarities	Belongs to the MAPEG family.

Ontologies

Keywords
Biological process	Leukotriene biosynthesis
Cellular component	Endoplasmic reticulum Membrane Microsome
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glutathione biosynthetic process Inferred from direct assay Ref.1. Source: UniProtKB leukotriene biosynthetic process Inferred from direct assay Ref.1PubMed 9092565. Source: UniProtKB positive regulation of catalytic activity Inferred from electronic annotation. Source: GOC xenobiotic metabolic process Traceable author statement. Source: Reactome
Cellular_component	endoplasmic reticulum membrane Traceable author statement. Source: Reactome integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nuclear envelope Inferred from Biological aspect of Ancestor. Source: RefGenome plasma membrane Inferred from direct assay PubMed 9092565. Source: UniProtKB
Molecular_function	enzyme activator activity Inferred from electronic annotation. Source: InterPro glutathione peroxidase activity Inferred from Biological aspect of Ancestor. Source: RefGenome glutathione transferase activity Inferred from direct assay Ref.1. Source: UniProtKB leukotriene-C4 synthase activity Inferred from direct assay Ref.1PubMed 9092565. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q99735-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q99735-2)

The sequence of this isoform differs from the canonical sequence as follows:
     53-147: QQNCVEFYPI...NIAKKLRRQF → HFCYLSGSGVHIWPSPILLGIFRSC

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 147	147	Microsomal glutathione S-transferase 2		PRO_0000217740
Regions
	Transmembrane	6 – 26	21	Helical; Potential
	Transmembrane	59 – 79	21	Helical; Potential
	Transmembrane	111 – 131	21	Helical; Potential
Natural variations
	Alternative sequence	53 – 147	95	QQNCV…LRRQF → HFCYLSGSGVHIWPSPILLG IFRSC in isoform 2.		VSP_044538
	Natural variant	101	1	A → V. Ref.3 Corresponds to variant rs8192111 [ dbSNP | Ensembl ].		VAR_019997

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: D0E89B46885D16EF	FASTA	147	16,621
	10 20 30 40 50 60 MAGNSILLAA VSILSACQQS YFALQVGKAR LKYKVTPPAV TGSPEFERVF RAQQNCVEFY 70 80 90 100 110 120 PIFIITLWMA GWYFNQVFAT CLGLVYIYGR HLYFWGYSEA AKKRITGFRL SLGILALLTL 130 140 LGALGIANSF LDEYLDLNIA KKLRRQF
	Isoform 2 [UniParc]. Checksum: CABF1D4ECFAD2B79	FASTA	77	8,359
	10 20 30 40 50 60 MAGNSILLAA VSILSACQQS YFALQVGKAR LKYKVTPPAV TGSPEFERVF RAHFCYLSGS 70 GVHIWPSPIL LGIFRSC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of a novel human microsomal glutathione S-transferase with leukotriene C4 synthase activity and significant sequence identity to 5-lipoxygenase-activating protein and leukotriene C4 synthase." Jakobsson P.-J., Mancini J.A., Ford-Hutchinson A.W. J. Biol. Chem. 271:22203-22210(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]	NIEHS SNPs program Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT VAL-101.
[4]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Lung and Neuroblastoma.
[6]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Web resources

NIEHS-SNPs

Cross-references

Sequence databases
EMBL GenBank DDBJ	U77604 mRNA. Translation: AAC51768.1. CR407640 mRNA. Translation: CAG28568.1. AY341028 Genomic DNA. Translation: AAP88934.1. Sequence problems. AC108053 Genomic DNA. No translation available. AC112236 Genomic DNA. No translation available. BC025416 mRNA. Translation: AAH25416.1. BG519599 mRNA. No translation available.
IPI	IPI00017767. IPI00965041.
RefSeq	NP_001191295.1. NM_001204366.1. NP_001191297.1. NM_001204368.1. NP_002404.1. NM_002413.4.
UniGene	Hs.81874.
3D structure databases
ProteinModelPortal	Q99735.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000265498.
PTM databases
PhosphoSite	Q99735.
Polymorphism databases
DMDM	2842764.
Proteomic databases
PaxDb	Q99735.
PRIDE	Q99735.
Protocols and materials databases
DNASU	4258.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000265498; ENSP00000265498; ENSG00000085871. ENST00000503816; ENSP00000423008; ENSG00000085871. ENST00000506797; ENSP00000424278; ENSG00000085871.
GeneID	4258.
KEGG	hsa:4258.
UCSC	uc003ihy.3. human.
Organism-specific databases
CTD	4258.
GeneCards	GC04P140586.
HGNC	HGNC:7063. MGST2.
HPA	HPA010707.
MIM	601733. gene.
neXtProt	NX_Q99735.
PharmGKB	PA30792.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG77136.
HOGENOM	HOG000116372.
HOVERGEN	HBG105513.
InParanoid	Q99735.
KO	K00799.
OMA	LYIYARH.
OrthoDB	EOG4V1729.
PhylomeDB	Q99735.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism.
Gene expression databases
ArrayExpress	Q99735.
Bgee	Q99735.
CleanEx	HS_MGST2.
Genevestigator	Q99735.
Family and domain databases
Gene3D	1.20.120.550. 1 hit.
InterPro	IPR001446. 5_LipOase_AP. IPR018295. FLAP/GST2/LTC4S_CS. IPR023352. MAPEG-like_dom. IPR001129. Membr-assoc_MAPEG. [Graphical view]
Pfam	PF01124. MAPEG. 1 hit. [Graphical view]
PRINTS	PR00488. 5LPOXGNASEAP.
PROSITE	PS01297. FLAP_GST2_LTC4S. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL1743185.
ChiTaRS	MGST2. human.
DrugBank	DB00143. Glutathione.
GenomeRNAi	4258.
NextBio	16797.
SOURCE	Search...

Entry information

Entry name

MGST2_HUMAN

Accession

Primary (citable) accession number: Q99735
Secondary accession number(s): D6RBB5, Q7Z5B8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 1, 1997
Last modified:	May 1, 2013
This is version 111 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.