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Q99735

- MGST2_HUMAN

UniProt

Q99735 - MGST2_HUMAN

Protein

Microsomal glutathione S-transferase 2

Gene

MGST2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Can catalyze the production of LTC4 from LTA4 and reduced glutathione. Can catalyze the conjugation of 1-chloro-2,4-dinitrobenzene with reduced glutathione.

    Catalytic activityi

    RX + glutathione = HX + R-S-glutathione.

    GO - Molecular functioni

    1. enzyme activator activity Source: InterPro
    2. glutathione peroxidase activity Source: RefGenome
    3. glutathione transferase activity Source: UniProtKB
    4. leukotriene-C4 synthase activity Source: UniProtKB

    GO - Biological processi

    1. glutathione biosynthetic process Source: UniProtKB
    2. glutathione derivative biosynthetic process Source: Reactome
    3. leukotriene biosynthetic process Source: UniProtKB
    4. oxidation-reduction process Source: GOC
    5. response to lipopolysaccharide Source: Ensembl
    6. response to organonitrogen compound Source: Ensembl
    7. small molecule metabolic process Source: Reactome
    8. xenobiotic metabolic process Source: Reactome

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Leukotriene biosynthesis

    Enzyme and pathway databases

    ReactomeiREACT_6926. Glutathione conjugation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Microsomal glutathione S-transferase 2 (EC:2.5.1.18)
    Short name:
    Microsomal GST-2
    Alternative name(s):
    Microsomal GST-II
    Gene namesi
    Name:MGST2
    Synonyms:GST2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 4

    Organism-specific databases

    HGNCiHGNC:7063. MGST2.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: Reactome
    2. integral component of membrane Source: UniProtKB-KW
    3. intracellular membrane-bounded organelle Source: UniProtKB
    4. nuclear envelope Source: RefGenome
    5. plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA30792.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 147147Microsomal glutathione S-transferase 2PRO_0000217740Add
    BLAST

    Proteomic databases

    MaxQBiQ99735.
    PaxDbiQ99735.
    PRIDEiQ99735.

    PTM databases

    PhosphoSiteiQ99735.

    Expressioni

    Tissue specificityi

    Liver, spleen, skeletal muscle, heart, adrenals, pancreas, prostate, testis, fetal liver, and fetal spleen. Very low expression in lung, brain, placenta and bone marrow.

    Gene expression databases

    BgeeiQ99735.
    CleanExiHS_MGST2.
    GenevestigatoriQ99735.

    Organism-specific databases

    HPAiHPA010707.

    Interactioni

    Subunit structurei

    Homotrimer.

    Protein-protein interaction databases

    STRINGi9606.ENSP00000265498.

    Structurei

    3D structure databases

    ProteinModelPortaliQ99735.
    SMRiQ99735. Positions 3-131.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei6 – 2621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei59 – 7921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei111 – 13121HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the MAPEG family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG77136.
    HOGENOMiHOG000116372.
    HOVERGENiHBG105513.
    InParanoidiQ99735.
    KOiK00799.
    OMAiLYIYARH.
    OrthoDBiEOG7GXPD5.
    PhylomeDBiQ99735.
    TreeFamiTF105328.

    Family and domain databases

    Gene3Di1.20.120.550. 1 hit.
    InterProiIPR001446. 5_LipOase_AP.
    IPR018295. FLAP/GST2/LTC4S_CS.
    IPR023352. MAPEG-like_dom.
    IPR001129. Membr-assoc_MAPEG.
    [Graphical view]
    PfamiPF01124. MAPEG. 1 hit.
    [Graphical view]
    PRINTSiPR00488. 5LPOXGNASEAP.
    PROSITEiPS01297. FLAP_GST2_LTC4S. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q99735-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAGNSILLAA VSILSACQQS YFALQVGKAR LKYKVTPPAV TGSPEFERVF    50
    RAQQNCVEFY PIFIITLWMA GWYFNQVFAT CLGLVYIYGR HLYFWGYSEA 100
    AKKRITGFRL SLGILALLTL LGALGIANSF LDEYLDLNIA KKLRRQF 147
    Length:147
    Mass (Da):16,621
    Last modified:May 1, 1997 - v1
    Checksum:iD0E89B46885D16EF
    GO
    Isoform 2 (identifier: Q99735-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         53-147: QQNCVEFYPI...NIAKKLRRQF → HFCYLSGSGVHIWPSPILLGIFRSC

    Note: No experimental confirmation available.

    Show »
    Length:77
    Mass (Da):8,359
    Checksum:iCABF1D4ECFAD2B79
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti101 – 1011A → V.1 Publication
    Corresponds to variant rs8192111 [ dbSNP | Ensembl ].
    VAR_019997

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei53 – 14795QQNCV…LRRQF → HFCYLSGSGVHIWPSPILLG IFRSC in isoform 2. 1 PublicationVSP_044538Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U77604 mRNA. Translation: AAC51768.1.
    CR407640 mRNA. Translation: CAG28568.1.
    AY341028 Genomic DNA. Translation: AAP88934.1. Sequence problems.
    AC108053 Genomic DNA. No translation available.
    AC112236 Genomic DNA. No translation available.
    BC025416 mRNA. Translation: AAH25416.1.
    BG519599 mRNA. No translation available.
    CCDSiCCDS3749.1. [Q99735-1]
    CCDS56339.1. [Q99735-2]
    RefSeqiNP_001191295.1. NM_001204366.1. [Q99735-1]
    NP_001191297.1. NM_001204368.1. [Q99735-2]
    NP_002404.1. NM_002413.4. [Q99735-1]
    UniGeneiHs.81874.

    Genome annotation databases

    EnsembliENST00000265498; ENSP00000265498; ENSG00000085871. [Q99735-1]
    ENST00000503816; ENSP00000423008; ENSG00000085871. [Q99735-1]
    ENST00000506797; ENSP00000424278; ENSG00000085871. [Q99735-2]
    GeneIDi4258.
    KEGGihsa:4258.
    UCSCiuc003ihy.3. human. [Q99735-1]

    Polymorphism databases

    DMDMi2842764.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Web resourcesi

    NIEHS-SNPs

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U77604 mRNA. Translation: AAC51768.1 .
    CR407640 mRNA. Translation: CAG28568.1 .
    AY341028 Genomic DNA. Translation: AAP88934.1 . Sequence problems.
    AC108053 Genomic DNA. No translation available.
    AC112236 Genomic DNA. No translation available.
    BC025416 mRNA. Translation: AAH25416.1 .
    BG519599 mRNA. No translation available.
    CCDSi CCDS3749.1. [Q99735-1 ]
    CCDS56339.1. [Q99735-2 ]
    RefSeqi NP_001191295.1. NM_001204366.1. [Q99735-1 ]
    NP_001191297.1. NM_001204368.1. [Q99735-2 ]
    NP_002404.1. NM_002413.4. [Q99735-1 ]
    UniGenei Hs.81874.

    3D structure databases

    ProteinModelPortali Q99735.
    SMRi Q99735. Positions 3-131.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9606.ENSP00000265498.

    Chemistry

    ChEMBLi CHEMBL1743185.
    DrugBanki DB00143. Glutathione.

    PTM databases

    PhosphoSitei Q99735.

    Polymorphism databases

    DMDMi 2842764.

    Proteomic databases

    MaxQBi Q99735.
    PaxDbi Q99735.
    PRIDEi Q99735.

    Protocols and materials databases

    DNASUi 4258.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000265498 ; ENSP00000265498 ; ENSG00000085871 . [Q99735-1 ]
    ENST00000503816 ; ENSP00000423008 ; ENSG00000085871 . [Q99735-1 ]
    ENST00000506797 ; ENSP00000424278 ; ENSG00000085871 . [Q99735-2 ]
    GeneIDi 4258.
    KEGGi hsa:4258.
    UCSCi uc003ihy.3. human. [Q99735-1 ]

    Organism-specific databases

    CTDi 4258.
    GeneCardsi GC04P140586.
    HGNCi HGNC:7063. MGST2.
    HPAi HPA010707.
    MIMi 601733. gene.
    neXtProti NX_Q99735.
    PharmGKBi PA30792.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG77136.
    HOGENOMi HOG000116372.
    HOVERGENi HBG105513.
    InParanoidi Q99735.
    KOi K00799.
    OMAi LYIYARH.
    OrthoDBi EOG7GXPD5.
    PhylomeDBi Q99735.
    TreeFami TF105328.

    Enzyme and pathway databases

    Reactomei REACT_6926. Glutathione conjugation.

    Miscellaneous databases

    ChiTaRSi MGST2. human.
    GeneWikii MGST2.
    GenomeRNAii 4258.
    NextBioi 16797.
    PROi Q99735.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q99735.
    CleanExi HS_MGST2.
    Genevestigatori Q99735.

    Family and domain databases

    Gene3Di 1.20.120.550. 1 hit.
    InterProi IPR001446. 5_LipOase_AP.
    IPR018295. FLAP/GST2/LTC4S_CS.
    IPR023352. MAPEG-like_dom.
    IPR001129. Membr-assoc_MAPEG.
    [Graphical view ]
    Pfami PF01124. MAPEG. 1 hit.
    [Graphical view ]
    PRINTSi PR00488. 5LPOXGNASEAP.
    PROSITEi PS01297. FLAP_GST2_LTC4S. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and characterization of a novel human microsomal glutathione S-transferase with leukotriene C4 synthase activity and significant sequence identity to 5-lipoxygenase-activating protein and leukotriene C4 synthase."
      Jakobsson P.-J., Mancini J.A., Ford-Hutchinson A.W.
      J. Biol. Chem. 271:22203-22210(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
      Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    3. NIEHS SNPs program
      Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT VAL-101.
    4. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
      Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
      , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
      Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Lung and Neuroblastoma.
    6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMGST2_HUMAN
    AccessioniPrimary (citable) accession number: Q99735
    Secondary accession number(s): D6RBB5, Q7Z5B8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 126 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 4
      Human chromosome 4: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3