Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Microsomal glutathione S-transferase 2

Gene

MGST2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Can catalyze the production of LTC4 from LTA4 and reduced glutathione. Can catalyze the conjugation of 1-chloro-2,4-dinitrobenzene with reduced glutathione.

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

GO - Molecular functioni

  1. enzyme activator activity Source: InterPro
  2. glutathione peroxidase activity Source: GO_Central
  3. glutathione transferase activity Source: UniProtKB
  4. leukotriene-C4 synthase activity Source: UniProtKB

GO - Biological processi

  1. glutathione biosynthetic process Source: UniProtKB
  2. glutathione derivative biosynthetic process Source: Reactome
  3. leukotriene biosynthetic process Source: UniProtKB
  4. response to lipopolysaccharide Source: Ensembl
  5. response to organonitrogen compound Source: Ensembl
  6. small molecule metabolic process Source: Reactome
  7. xenobiotic metabolic process Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Leukotriene biosynthesis

Enzyme and pathway databases

ReactomeiREACT_228214. Aflatoxin activation and detoxification.
REACT_6926. Glutathione conjugation.

Names & Taxonomyi

Protein namesi
Recommended name:
Microsomal glutathione S-transferase 2 (EC:2.5.1.18)
Short name:
Microsomal GST-2
Alternative name(s):
Microsomal GST-II
Gene namesi
Name:MGST2
Synonyms:GST2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 4

Organism-specific databases

HGNCiHGNC:7063. MGST2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei6 – 2621HelicalSequence AnalysisAdd
BLAST
Transmembranei59 – 7921HelicalSequence AnalysisAdd
BLAST
Transmembranei111 – 13121HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: Reactome
  2. integral component of membrane Source: UniProtKB-KW
  3. intracellular membrane-bounded organelle Source: UniProtKB
  4. nuclear envelope Source: GO_Central
  5. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA30792.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 147147Microsomal glutathione S-transferase 2PRO_0000217740Add
BLAST

Proteomic databases

MaxQBiQ99735.
PaxDbiQ99735.
PRIDEiQ99735.

PTM databases

PhosphoSiteiQ99735.

Expressioni

Tissue specificityi

Liver, spleen, skeletal muscle, heart, adrenals, pancreas, prostate, testis, fetal liver, and fetal spleen. Very low expression in lung, brain, placenta and bone marrow.

Gene expression databases

BgeeiQ99735.
CleanExiHS_MGST2.
GenevestigatoriQ99735.

Organism-specific databases

HPAiHPA010707.

Interactioni

Subunit structurei

Homotrimer.

Protein-protein interaction databases

STRINGi9606.ENSP00000265498.

Structurei

3D structure databases

ProteinModelPortaliQ99735.
SMRiQ99735. Positions 3-131.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MAPEG family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG77136.
GeneTreeiENSGT00430000030964.
HOGENOMiHOG000116372.
HOVERGENiHBG105513.
InParanoidiQ99735.
KOiK00799.
OMAiIFRAQQN.
OrthoDBiEOG7GXPD5.
PhylomeDBiQ99735.
TreeFamiTF105328.

Family and domain databases

Gene3Di1.20.120.550. 1 hit.
InterProiIPR001446. 5_LipOase_AP.
IPR018295. FLAP/GST2/LTC4S_CS.
IPR023352. MAPEG-like_dom.
IPR001129. Membr-assoc_MAPEG.
[Graphical view]
PfamiPF01124. MAPEG. 1 hit.
[Graphical view]
PRINTSiPR00488. 5LPOXGNASEAP.
PROSITEiPS01297. FLAP_GST2_LTC4S. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q99735-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGNSILLAA VSILSACQQS YFALQVGKAR LKYKVTPPAV TGSPEFERVF
60 70 80 90 100
RAQQNCVEFY PIFIITLWMA GWYFNQVFAT CLGLVYIYGR HLYFWGYSEA
110 120 130 140
AKKRITGFRL SLGILALLTL LGALGIANSF LDEYLDLNIA KKLRRQF
Length:147
Mass (Da):16,621
Last modified:May 1, 1997 - v1
Checksum:iD0E89B46885D16EF
GO
Isoform 2 (identifier: Q99735-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     53-147: QQNCVEFYPI...NIAKKLRRQF → HFCYLSGSGVHIWPSPILLGIFRSC

Note: No experimental confirmation available.

Show »
Length:77
Mass (Da):8,359
Checksum:iCABF1D4ECFAD2B79
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti101 – 1011A → V.1 Publication
Corresponds to variant rs8192111 [ dbSNP | Ensembl ].
VAR_019997

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei53 – 14795QQNCV…LRRQF → HFCYLSGSGVHIWPSPILLG IFRSC in isoform 2. 1 PublicationVSP_044538Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77604 mRNA. Translation: AAC51768.1.
CR407640 mRNA. Translation: CAG28568.1.
AY341028 Genomic DNA. Translation: AAP88934.1. Sequence problems.
AC108053 Genomic DNA. No translation available.
AC112236 Genomic DNA. No translation available.
BC025416 mRNA. Translation: AAH25416.1.
BG519599 mRNA. No translation available.
CCDSiCCDS3749.1. [Q99735-1]
CCDS56339.1. [Q99735-2]
RefSeqiNP_001191295.1. NM_001204366.1. [Q99735-1]
NP_001191297.1. NM_001204368.1. [Q99735-2]
NP_002404.1. NM_002413.4. [Q99735-1]
UniGeneiHs.81874.

Genome annotation databases

EnsembliENST00000265498; ENSP00000265498; ENSG00000085871. [Q99735-1]
ENST00000503816; ENSP00000423008; ENSG00000085871. [Q99735-1]
ENST00000506797; ENSP00000424278; ENSG00000085871. [Q99735-2]
ENST00000616265; ENSP00000482639; ENSG00000085871. [Q99735-1]
GeneIDi4258.
KEGGihsa:4258.
UCSCiuc003ihy.3. human. [Q99735-1]

Polymorphism databases

DMDMi2842764.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77604 mRNA. Translation: AAC51768.1.
CR407640 mRNA. Translation: CAG28568.1.
AY341028 Genomic DNA. Translation: AAP88934.1. Sequence problems.
AC108053 Genomic DNA. No translation available.
AC112236 Genomic DNA. No translation available.
BC025416 mRNA. Translation: AAH25416.1.
BG519599 mRNA. No translation available.
CCDSiCCDS3749.1. [Q99735-1]
CCDS56339.1. [Q99735-2]
RefSeqiNP_001191295.1. NM_001204366.1. [Q99735-1]
NP_001191297.1. NM_001204368.1. [Q99735-2]
NP_002404.1. NM_002413.4. [Q99735-1]
UniGeneiHs.81874.

3D structure databases

ProteinModelPortaliQ99735.
SMRiQ99735. Positions 3-131.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000265498.

Chemistry

ChEMBLiCHEMBL1743185.
DrugBankiDB01008. Busulfan.
DB00143. Glutathione.

PTM databases

PhosphoSiteiQ99735.

Polymorphism databases

DMDMi2842764.

Proteomic databases

MaxQBiQ99735.
PaxDbiQ99735.
PRIDEiQ99735.

Protocols and materials databases

DNASUi4258.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000265498; ENSP00000265498; ENSG00000085871. [Q99735-1]
ENST00000503816; ENSP00000423008; ENSG00000085871. [Q99735-1]
ENST00000506797; ENSP00000424278; ENSG00000085871. [Q99735-2]
ENST00000616265; ENSP00000482639; ENSG00000085871. [Q99735-1]
GeneIDi4258.
KEGGihsa:4258.
UCSCiuc003ihy.3. human. [Q99735-1]

Organism-specific databases

CTDi4258.
GeneCardsiGC04P140586.
HGNCiHGNC:7063. MGST2.
HPAiHPA010707.
MIMi601733. gene.
neXtProtiNX_Q99735.
PharmGKBiPA30792.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG77136.
GeneTreeiENSGT00430000030964.
HOGENOMiHOG000116372.
HOVERGENiHBG105513.
InParanoidiQ99735.
KOiK00799.
OMAiIFRAQQN.
OrthoDBiEOG7GXPD5.
PhylomeDBiQ99735.
TreeFamiTF105328.

Enzyme and pathway databases

ReactomeiREACT_228214. Aflatoxin activation and detoxification.
REACT_6926. Glutathione conjugation.

Miscellaneous databases

ChiTaRSiMGST2. human.
GeneWikiiMGST2.
GenomeRNAii4258.
NextBioi16797.
PROiQ99735.
SOURCEiSearch...

Gene expression databases

BgeeiQ99735.
CleanExiHS_MGST2.
GenevestigatoriQ99735.

Family and domain databases

Gene3Di1.20.120.550. 1 hit.
InterProiIPR001446. 5_LipOase_AP.
IPR018295. FLAP/GST2/LTC4S_CS.
IPR023352. MAPEG-like_dom.
IPR001129. Membr-assoc_MAPEG.
[Graphical view]
PfamiPF01124. MAPEG. 1 hit.
[Graphical view]
PRINTSiPR00488. 5LPOXGNASEAP.
PROSITEiPS01297. FLAP_GST2_LTC4S. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of a novel human microsomal glutathione S-transferase with leukotriene C4 synthase activity and significant sequence identity to 5-lipoxygenase-activating protein and leukotriene C4 synthase."
    Jakobsson P.-J., Mancini J.A., Ford-Hutchinson A.W.
    J. Biol. Chem. 271:22203-22210(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  3. NIEHS SNPs program
    Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT VAL-101.
  4. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Lung and Neuroblastoma.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiMGST2_HUMAN
AccessioniPrimary (citable) accession number: Q99735
Secondary accession number(s): D6RBB5, Q7Z5B8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 1997
Last modified: March 4, 2015
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.