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Protein

Nucleosome assembly protein 1-like 4

Gene

NAP1L4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as histone chaperone in nucleosome assembly.1 Publication

GO - Molecular functioni

  • nucleosome binding Source: UniProtKB
  • RNA binding Source: UniProtKB
  • unfolded protein binding Source: ProtInc

GO - Biological processi

  • nucleosome assembly Source: UniProtKB

Keywordsi

Molecular functionChaperone

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleosome assembly protein 1-like 4
Alternative name(s):
Nucleosome assembly protein 21 Publication
Short name:
NAP-21 Publication
Gene namesi
Name:NAP1L4Imported
Synonyms:NAP21 Publication
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000205531.12.
HGNCiHGNC:7640. NAP1L4.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi4676.
OpenTargetsiENSG00000205531.
PharmGKBiPA31442.

Polymorphism and mutation databases

BioMutaiNAP1L4.
DMDMi2498672.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001856582 – 375Nucleosome assembly protein 1-like 4Add BLAST374

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei5PhosphoserineCombined sources1
Modified residuei7PhosphoserineCombined sources1
Modified residuei12PhosphoserineCombined sources1
Modified residuei51PhosphothreonineCombined sources1
Modified residuei53PhosphoserineBy similarity1
Modified residuei54PhosphoserineCombined sources1
Modified residuei58PhosphothreonineCombined sources1
Modified residuei105N6-acetyllysineBy similarity1
Modified residuei125PhosphoserineCombined sources1
Modified residuei146N6-acetyllysineBy similarity1
Modified residuei304PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated at the G0/G1 boundary but it is not phosphorylated in S-phase. Phosphorylated protein remains in the cytoplasm in a complex with histones during the G0/G1 transition, whereas dephosphorylation triggers its transport into the nucleus at the G1/S-boundary.1 Publication
Polyglutamylated by TTLL4, a modification that occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Some residues may also be monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human (By similarity).By similarityCurated

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ99733.
MaxQBiQ99733.
PaxDbiQ99733.
PeptideAtlasiQ99733.
PRIDEiQ99733.

PTM databases

iPTMnetiQ99733.
PhosphoSitePlusiQ99733.
SwissPalmiQ99733.

Miscellaneous databases

PMAP-CutDBiQ99733.

Expressioni

Tissue specificityi

Ubiquitous. Biallelically expressed in fetal and adult tissues. Highest levels in testis.1 Publication

Gene expression databases

BgeeiENSG00000205531.
CleanExiHS_NAP1L4.
ExpressionAtlasiQ99733. baseline and differential.
GenevisibleiQ99733. HS.

Organism-specific databases

HPAiCAB011567.

Interactioni

Subunit structurei

Interacts with core (H2A, H2B, H3, H4) and linker (H1) histones.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
tatP046083EBI-2255116,EBI-6164389From Human immunodeficiency virus type 1 group M subtype B (isolate HXB2).

GO - Molecular functioni

  • unfolded protein binding Source: ProtInc

Protein-protein interaction databases

BioGridi110757. 76 interactors.
IntActiQ99733. 33 interactors.
MINTiMINT-5001909.
STRINGi9606.ENSP00000369915.

Structurei

3D structure databases

ProteinModelPortaliQ99733.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi265 – 271Nuclear localization signalSequence analysis7

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi126 – 131Poly-Glu6
Compositional biasi301 – 310Asp/Glu-rich (acidic)10
Compositional biasi347 – 370Asp/Glu-rich (acidic)Add BLAST24

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1507. Eukaryota.
ENOG410XQN9. LUCA.
GeneTreeiENSGT00480000042668.
HOGENOMiHOG000171827.
HOVERGENiHBG052653.
InParanoidiQ99733.
KOiK11282.
OMAiSQPSECK.
OrthoDBiEOG091G0HVV.
PhylomeDBiQ99733.
TreeFamiTF314349.

Family and domain databases

InterProiView protein in InterPro
IPR037231. NAP-like_sf.
IPR002164. NAP_family.
PANTHERiPTHR11875. PTHR11875. 1 hit.
PfamiView protein in Pfam
PF00956. NAP. 1 hit.
SUPFAMiSSF143113. SSF143113. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q99733-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADHSFSDGV PSDSVEAAKN ASNTEKLTDQ VMQNPRVLAA LQERLDNVPH
60 70 80 90 100
TPSSYIETLP KAVKRRINAL KQLQVRCAHI EAKFYEEVHD LERKYAALYQ
110 120 130 140 150
PLFDKRREFI TGDVEPTDAE SEWHSENEEE EKLAGDMKSK VVVTEKAAAT
160 170 180 190 200
AEEPDPKGIP EFWFTIFRNV DMLSELVQEY DEPILKHLQD IKVKFSDPGQ
210 220 230 240 250
PMSFVLEFHF EPNDYFTNSV LTKTYKMKSE PDKADPFSFE GPEIVDCDGC
260 270 280 290 300
TIDWKKGKNV TVKTIKKKQK HKGRGTVRTI TKQVPNESFF NFFNPLKASG
310 320 330 340 350
DGESLDEDSE FTLASDFEIG HFFRERIVPR AVLYFTGEAI EDDDNFEEGE
360 370
EGEEEELEGD EEGEDEDDAE INPKV
Length:375
Mass (Da):42,823
Last modified:May 1, 1997 - v1
Checksum:i788E1F9F5BC8FD45
GO
Isoform 2 (identifier: Q99733-2) [UniParc]FASTAAdd to basket
Also known as: NAP1L4b1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     375-375: V → KEPSQPAECKQQ

Show »
Length:386
Mass (Da):44,079
Checksum:i4B758A68C22F9D9E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti107R → K in BAG35491 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_053910375V → KEPSQPAECKQQ in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77456 mRNA. Translation: AAC50870.1.
AB500094 mRNA. Translation: BAH95827.1.
AK312599 mRNA. Translation: BAG35491.1.
AC131971 Genomic DNA. No translation available.
BC022090 mRNA. Translation: AAH22090.1.
CCDSiCCDS41599.1. [Q99733-1]
RefSeqiNP_005960.1. NM_005969.3. [Q99733-1]
UniGeneiHs.731784.

Genome annotation databases

EnsembliENST00000380542; ENSP00000369915; ENSG00000205531. [Q99733-1]
ENST00000526115; ENSP00000436397; ENSG00000205531. [Q99733-2]
ENST00000616696; ENSP00000478654; ENSG00000273562. [Q99733-2]
ENST00000619695; ENSP00000483728; ENSG00000273562. [Q99733-1]
ENST00000620138; ENSP00000481412; ENSG00000205531. [Q99733-2]
ENST00000632791; ENSP00000487984; ENSG00000273562. [Q99733-2]
GeneIDi4676.
KEGGihsa:4676.
UCSCiuc001lxc.4. human. [Q99733-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNP1L4_HUMAN
AccessioniPrimary (citable) accession number: Q99733
Secondary accession number(s): B2R6J4, F5HFY4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 1997
Last modified: October 25, 2017
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families