Reviewed, UniProtKB/Swiss-Prot Q99733 (NP1L4_HUMAN)

Names and origin

Protein names

Recommended name:
    Nucleosome assembly protein 1-like 4
Alternative name(s):
    Nucleosome assembly protein 2
      Short name=NAP2

Gene names

Name:	NAP1L4
Synonyms:	NAP2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subcellular location	Nucleus.
Tissue specificity	Ubiquitous. Biallelically expressed in fetal and adult tissues. Highest levels in testis.
Sequence similarities	Belongs to the nucleosome assembly protein (NAP) family.

Ontologies

Keywords
Cellular component	Nucleus
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	nucleosome assembly Traceable author statement. Source: ProtInc
Cellular component	chromatin assembly complex Traceable author statement. Source: ProtInc cytoplasm Traceable author statement. Source: ProtInc
Molecular function	unfolded protein binding Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 375	375	Nucleosome assembly protein 1-like 4		PRO_0000185658
Regions
	Motif	265 – 271	7	Nuclear localization signal Potential
	Compositional bias	126 – 131	6	Poly-Glu
	Compositional bias	301 – 310	10	Asp/Glu-rich (acidic)
	Compositional bias	347 – 370	24	Asp/Glu-rich (acidic)
Amino acid modifications
	Modified residue	5	1	Phosphoserine
	Modified residue	51	1	Phosphothreonine
	Modified residue	53	1	Phosphoserine
	Modified residue	125	1	Phosphoserine
	Modified residue	139	1	Phosphoserine

Sequences

	Sequence		Length	Mass (Da)
	Q99733-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 788E1F9F5BC8FD45	FASTA	375	42,823
	10 20 30 40 50 60 MADHSFSDGV PSDSVEAAKN ASNTEKLTDQ VMQNPRVLAA LQERLDNVPH TPSSYIETLP 70 80 90 100 110 120 KAVKRRINAL KQLQVRCAHI EAKFYEEVHD LERKYAALYQ PLFDKRREFI TGDVEPTDAE 130 140 150 160 170 180 SEWHSENEEE EKLAGDMKSK VVVTEKAAAT AEEPDPKGIP EFWFTIFRNV DMLSELVQEY 190 200 210 220 230 240 DEPILKHLQD IKVKFSDPGQ PMSFVLEFHF EPNDYFTNSV LTKTYKMKSE PDKADPFSFE 250 260 270 280 290 300 GPEIVDCDGC TIDWKKGKNV TVKTIKKKQK HKGRGTVRTI TKQVPNESFF NFFNPLKASG 310 320 330 340 350 360 DGESLDEDSE FTLASDFEIG HFFRERIVPR AVLYFTGEAI EDDDNFEEGE EGEEEELEGD 370 EEGEDEDDAE INPKV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel human homologue of yeast nucleosome assembly protein, 65 kb centromeric to the p57KIP2 gene, is biallelically expressed in fetal and adult tissues." Hu R.-J., Lee M.P., Johnson L.A., Feinberg A.P. Hum. Mol. Genet. 5:1743-1748(1996) [PubMed: 8923002] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Muscle.
[3]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-5 AND SER-125, MASS SPECTROMETRY. Tissue: Epithelium.
[4]	"Phosphoproteome analysis of the human mitotic spindle." Nousiainen M., Sillje H.H.W., Sauer G., Nigg E.A., Koerner R. Proc. Natl. Acad. Sci. U.S.A. 103:5391-5396(2006) [PubMed: 16565220] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-53 AND SER-125, MASS SPECTROMETRY. Tissue: Epithelium.
[5]	"Toward a global characterization of the phosphoproteome in prostate cancer cells: identification of phosphoproteins in the LNCaP cell line." Giorgianni F., Zhao Y., Desiderio D.M., Beranova-Giorgianni S. Electrophoresis 28:2027-2034(2007) [PubMed: 17487921] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-125, MASS SPECTROMETRY.
[6]	"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra." Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D. J. Proteome Res. 6:4150-4162(2007) [PubMed: 17924679] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-51, MASS SPECTROMETRY. Tissue: Epithelium.
[7]	"Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry." Molina H., Horn D.M., Tang N., Mathivanan S., Pandey A. Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007) [PubMed: 17287340] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-125 AND SER-139, MASS SPECTROMETRY.
[8]	"Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography." Han G., Ye M., Zhou H., Jiang X., Feng S., Jiang X., Tian R., Wan D., Zou H., Gu J. Proteomics 8:1346-1361(2008) [PubMed: 18318008] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-125, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U77456 mRNA. Translation: AAC50870.1. BC022090 mRNA. Translation: AAH22090.1.
RefSeq	NP_005960.1.
UniGene	Hs.501684 Hs.695395
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q99733.
Genome annotation databases
Ensembl	ENSG00000205531. Homo sapiens. [Contig view]
GeneID	4676.
KEGG	hsa:4676.
Organism-specific databases
H-InvDB	HIX0009370.
HGNC	HGNC:7640. NAP1L4.
HPA	CAB011567.
MIM	601651. gene.
PharmGKB	PA31442.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	Q99733.
HOVERGEN	Q99733.
Gene expression databases
ArrayExpress	Q99733.
CleanEx	HS_NAP1L4.
GermOnline	ENSG00000205531. Homo sapiens.
Family and domain databases
InterPro	IPR002164. NAP_family. [Graphical view]
PANTHER	PTHR11875. NAP_family. 1 hit.
Pfam	PF00956. NAP. 1 hit. [Graphical view]
BLOCKS	Search...
ProtoNet	Search...
Other Resources
LinkHub	Q99733.
NextBio	18024.
SOURCE	Search...

Entry information

Entry name

NP1L4_HUMAN

Accession

Primary (citable) accession number: Q99733

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 1, 1997
Last modified:	November 4, 2008
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)