Reviewed, UniProtKB/Swiss-Prot Q99727 (TIMP4_HUMAN)

Names and origin

Protein names

Recommended name:
    Metalloproteinase inhibitor 4
Alternative name(s):
    Tissue inhibitor of metalloproteinases 4
      Short name=TIMP-4

Gene names

Name:

TIMP4

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	224 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP-9.
Subcellular location	Secreted.
Tissue specificity	Abundant in heart and present at low levels in many other tissues.
Sequence similarities	Belongs to the protease inhibitor I35 (TIMP) family. [View classification] Contains 1 NTR domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Metalloenzyme inhibitor Metalloprotease inhibitor
PTM	Disulfide bond
Gene Ontology (GO)
Cellular component	proteinaceous extracellular matrix Inferred from electronic annotation. Source: InterPro
Molecular function	metalloendopeptidase inhibitor activity Ref.1 Non-traceable author statement. Source: UniProtKB protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Chain	28 – 224	197	Metalloproteinase inhibitor 4		PRO_0000034349
Regions
	Domain	30 – 156	127	NTR
Amino acid modifications
	Disulfide bond	30 ↔ 102		By similarity
	Disulfide bond	32 ↔ 131		By similarity
	Disulfide bond	42 ↔ 156		By similarity
	Disulfide bond	158 ↔ 205		By similarity
	Disulfide bond	163 ↔ 168		By similarity
	Disulfide bond	176 ↔ 197		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q99727-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 39C7187D78D99442	FASTA	224	25,503
	10 20 30 40 50 60 MPGSPRPAPS WVLLLRLLAL LRPPGLGEAC SCAPAHPQQH ICHSALVIRA KISSEKVVPA 70 80 90 100 110 120 SADPADTEKM LRYEIKQIKM FKGFEKVKDV QYIYTPFDSS LCGVKLEANS QKQYLLTGQV 130 140 150 160 170 180 LSDGKVFIHL CNYIEPWEDL SLVQRESLNH HYHLNCGCQI TTCYTVPCTI SAPNECLWTD 190 200 210 220 WLLERKLYGY QAQHYVCMKH VDGTCSWYRG HLPLRKEFVD IVQP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of human tissue inhibitor of metalloproteinase 4." Greene J., Wang M., Liu Y.E., Raymond L.A., Rosen C., Shi Y.E. J. Biol. Chem. 271:30375-30380(1996) [PubMed: 8939999] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Heart.
[2]	"Cloning of the human tissue inhibitor of metalloproteinase-4 gene (TIMP4) and localization of the TIMP4 and timp4 genes to human chromosome 3p25 and mouse chromosome 6, respectively." Olson T.M., Hirohata S., Ye J., Leco K., Seldin M.F., Apte S.S. Genomics 51:148-151(1998) [PubMed: 9693046] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U76456 mRNA. Translation: AAB40391.1. AF057532 , AF057528, AF057529, AF057530, AF057531 Genomic DNA. Translation: AAC34422.1. BT019627 mRNA. Translation: AAV38433.1. BC010553 mRNA. Translation: AAH10553.1.
IPI	IPI00017745.
RefSeq	NP_003247.1.
UniGene	Hs.591665
3D structure databases
HSSP	HSSP built from PDB template 1BR9 based on UniProtKB P16035.
ModBase	Search...
Protein family/group databases
MEROPS	I35.004.
Proteomic databases
PRIDE	Q99727.
Genome annotation databases
Ensembl	ENSG00000157150. Homo sapiens. [Contig view]
GeneID	7079.
KEGG	hsa:7079.
Organism-specific databases
GeneCards	GC03M012169.
H-InvDB	HIX0003063.
HGNC	HGNC:11823. TIMP4.
HPA	CAB002771.
MIM	601915. gene.
PharmGKB	PA36529.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q99727.
HOVERGEN	Q99727.
OMA	Q99727. LCNYIEP.
Gene expression databases
ArrayExpress	Q99727.
Bgee	Q99727.
CleanEx	HS_TIMP4.
GermOnline	ENSG00000157150. Homo sapiens.
Family and domain databases
InterPro	IPR001134. Netrin_domain. IPR001820. Prot_inh_TIMP. IPR015614. TIMP4. [Graphical view]
PANTHER	PTHR11844. Prot_inh_TIMP. 1 hit. PTHR11844:SF8. TIMP4. 1 hit.
Pfam	PF00965. TIMP. 1 hit. [Graphical view]
SMART	SM00206. NTR. 1 hit. [Graphical view]
PROSITE	PS50189. NTR. 1 hit. PS00288. TIMP. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	27689.
SOURCE	Search...

Entry information

Entry name

TIMP4_HUMAN

Accession

Primary (citable) accession number: Q99727

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	May 1, 1997
Last modified:	June 16, 2009
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)