Q99712 (IRK15_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-sensitive inward rectifier potassium channel 15

Alternative name(s):
Inward rectifier K(+) channel Kir1.3
Inward rectifier K(+) channel Kir4.2
Potassium channel, inwardly rectifying subfamily J member 15

Gene names

Name:	KCNJ15
Synonyms:	KCNJ14

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium.
Subunit structure	Interacts with INADL. Ref.7
Subcellular location	Membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ15 subfamily. [View classification]

Ontologies

Keywords
Biological process	Ion transport Potassium transport Transport
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Ligand	Potassium
Molecular function	Ion channel Voltage-gated channel
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	synaptic transmission Traceable author statement. Source: Reactome
Cellular_component	integral to plasma membrane Traceable author statement Ref.2. Source: ProtInc
Molecular_function	inward rectifier potassium channel activity Traceable author statement Ref.2. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 375	375	ATP-sensitive inward rectifier potassium channel 15		PRO_0000154972
Regions
	Topological domain	1 – 63	63	Cytoplasmic By similarity
	Transmembrane	64 – 88	25	Helical; Name=M1; By similarity
	Topological domain	89 – 113	25	Extracellular By similarity
	Intramembrane	114 – 125	12	Helical; Pore-forming; Name=H5; By similarity
	Intramembrane	126 – 132	7	Pore-forming; By similarity
	Topological domain	133 – 141	9	Extracellular By similarity
	Transmembrane	142 – 163	22	Helical; Name=M2; By similarity
	Topological domain	164 – 375	212	Cytoplasmic By similarity
	Motif	127 – 132	6	Selectivity filter By similarity
Sites
	Site	157	1	Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium By similarity
Natural variations
	Natural variant	30	1	M → L. Corresponds to variant rs3746875 [ dbSNP | Ensembl ].		VAR_025523
	Natural variant	71	1	A → T in a breast cancer sample; somatic mutation. Ref.8		VAR_036427
	Natural variant	98	1	G → D. Ref.2 Ref.3 Corresponds to variant rs2230033 [ dbSNP | Ensembl ].		VAR_019728
Experimental info
	Sequence conflict	235	1	V → A in CAA71734. Ref.1
	Sequence conflict	245	1	S → G in BAA13326. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q99712 [UniParc]. Last modified October 11, 2005. Version 2. Checksum: CECCB4EDF827B24D	FASTA	375	42,577
	10 20 30 40 50 60 MDAIHIGMSS TPLVKHTAGA GLKANRPRVM SKSGHSNVRI DKVDGIYLLY LQDLWTTVID 70 80 90 100 110 120 MKWRYKLTLF AATFVMTWFL FGVIYYAIAF IHGDLEPGEP ISNHTPCIMK VDSLTGAFLF 130 140 150 160 170 180 SLESQTTIGY GVRSITEECP HAIFLLVAQL VITTLIEIFI TGTFLAKIAR PKKRAETIKF 190 200 210 220 230 240 SHCAVITKQN GKLCLVIQVA NMRKSLLIQC QLSGKLLQTH VTKEGERILL NQATVKFHVD 250 260 270 280 290 300 SSSESPFLIL PMTFYHVLDE TSPLRDLTPQ NLKEKEFELV VLLNATVEST SAVCQSRTSY 310 320 330 340 350 360 IPEEIYWGFE FVPVVSLSKN GKYVADFSQF EQIRKSPDCT FYCADSEKQQ LEEKYRQEDQ 370 RERELRTLLL QQSNV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A new inward rectifier potassium channel gene (KCNJ15) localized on chromosome 21 in the Down syndrome chromosome region 1 (DCR1)." Gosset P., Ghezala G.A., Korn B., Yaspo M.-L., Poutska A., Lehrach H., Sinet P.-M., Creau N. Genomics 44:237-241(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cloning and characterization of two K+ inward rectifier (Kir) 1.1 potassium channel homologs from human kidney (Kir1.2 and Kir1.3)." Shuck M.E., Piser T.M., Bock J.H., Slightom J.L., Lee K.S., Bienkowski M.J. J. Biol. Chem. 272:586-593(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ASP-98. Tissue: Kidney.
[3]	Ohira M., Seki N., Nagase T., Suzuki E., Nomura N., Ohara O., Hattori M., Sakaki Y., Eki T., Murakami Y., Saito T., Ichikawa H., Ohki M. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ASP-98. Tissue: Kidney.
[4]	"The DNA sequence of human chromosome 21." Hattori M., Fujiyama A., Taylor T.D., Watanabe H., Yada T., Park H.-S., Toyoda A., Ishii K., Totoki Y., Choi D.-K., Groner Y., Soeda E., Ohki M., Takagi T., Sakaki Y., Taudien S., Blechschmidt K., Polley A. , Menzel U., Delabar J., Kumpf K., Lehmann R., Patterson D., Reichwald K., Rump A., Schillhabel M., Schudy A., Zimmermann W., Rosenthal A., Kudoh J., Shibuya K., Kawasaki K., Asakawa S., Shintani A., Sasaki T., Nagamine K., Mitsuyama S., Antonarakis S.E., Minoshima S., Shimizu N., Nordsiek G., Hornischer K., Brandt P., Scharfe M., Schoen O., Desario A., Reichelt J., Kauer G., Bloecker H., Ramser J., Beck A., Klages S., Hennig S., Riesselmann L., Dagand E., Wehrmeyer S., Borzym K., Gardiner K., Nizetic D., Francis F., Lehrach H., Reinhardt R., Yaspo M.-L. Nature 405:311-319(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[7]	"CIPP, a novel multivalent PDZ domain protein, selectively interacts with Kir4.0 family members, NMDA receptor subunits, neurexins, and neuroligins." Kurschner C., Mermelstein P.G., Holden W.T., Surmeier D.J. Mol. Cell. Neurosci. 11:161-172(1998) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH INADL.
[8]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] THR-71.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y10745 mRNA. Translation: CAA71734.1. U73191 mRNA. Translation: AAC50922.1. D87291 mRNA. Translation: BAA13326.1. AP001434 Genomic DNA. No translation available. CH471079 Genomic DNA. Translation: EAX09683.1. CH471079 Genomic DNA. Translation: EAX09684.1. BC013327 mRNA. Translation: AAH13327.1.
IPI	IPI00064771.
RefSeq	NP_001263364.1. NM_001276435.1. NP_001263365.1. NM_001276436.1. NP_001263366.1. NM_001276437.1. NP_001263367.1. NM_001276438.1. NP_001263368.1. NM_001276439.1. NP_002234.2. NM_002243.4. NP_733932.1. NM_170736.2. NP_733933.1. NM_170737.2.
UniGene	Hs.411299.
3D structure databases
ProteinModelPortal	Q99712.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-103775.
STRING	9606.ENSP00000331698.
PTM databases
PhosphoSite	Q99712.
Polymorphism databases
DMDM	77416869.
Proteomic databases
PaxDb	Q99712.
PRIDE	Q99712.
Protocols and materials databases
DNASU	3772.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000328656; ENSP00000331698; ENSG00000157551. ENST00000398930; ENSP00000381904; ENSG00000157551. ENST00000398932; ENSP00000381905; ENSG00000157551. ENST00000398934; ENSP00000381907; ENSG00000157551. ENST00000398938; ENSP00000381911; ENSG00000157551.
GeneID	3772.
KEGG	hsa:3772.
UCSC	uc002ywv.3. human.
Organism-specific databases
CTD	3772.
GeneCards	GC21P039529.
HGNC	HGNC:6261. KCNJ15.
HPA	HPA016702.
MIM	602106. gene.
neXtProt	NX_Q99712.
PharmGKB	PA30046.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG289501.
HOGENOM	HOG000237326.
HOVERGEN	HBG006178.
InParanoid	Q99712.
KO	K05008.
OMA	VRVANMR.
OrthoDB	EOG41JZCP.
PhylomeDB	Q99712.
Enzyme and pathway databases
Reactome	REACT_13685. Neuronal System.
Gene expression databases
ArrayExpress	Q99712.
Bgee	Q99712.
CleanEx	HS_KCNJ14. HS_KCNJ15.
Genevestigator	Q99712.
GermOnline	ENSG00000157551. Homo sapiens.
Family and domain databases
Gene3D	2.60.40.1400. 1 hit.
InterPro	IPR014756. Ig_E-set. IPR016449. K_chnl_inward-rec_Kir. IPR003270. K_chnl_inward-rec_Kir1.3. IPR013518. K_chnl_inward-rec_Kir_cyto. [Graphical view]
PANTHER	PTHR11767. PTHR11767. 1 hit. PTHR11767:SF20. PTHR11767:SF20. 1 hit.
Pfam	PF01007. IRK. 1 hit. [Graphical view]
PIRSF	PIRSF005465. GIRK_kir. 1 hit.
PRINTS	PR01323. KIR13CHANNEL. PR01320. KIRCHANNEL.
SUPFAM	SSF81296. Ig_E-set. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	3772.
NextBio	14791.
SOURCE	Search...

Entry information

Entry name

IRK15_HUMAN

Accession

Primary (citable) accession number: Q99712
Secondary accession number(s): D3DSH5 , O00564, Q96L28, Q99446

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	October 11, 2005
Last modified:	May 1, 2013
This is version 118 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.