Q99705 (MCHR1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Melanin-concentrating hormone receptor 1
Short name=MCH receptor 1
Short name=MCH-R1
Short name=MCHR-1

Alternative name(s):
G-protein coupled receptor 24
MCH-1R
Short name=MCH1R
Short name=MCHR
SLC-1
Somatostatin receptor-like protein

Gene names

Name:	MCHR1
Synonyms:	GPR24, SLC1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	422 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for melanin-concentrating hormone, coupled to both G proteins that inhibit adenylyl cyclase and G proteins that activate phosphoinositide hydrolysis. Ref.11
Subunit structure	Interacts with NCDN. Ref.12
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Highest level in brain, particularly in the frontal cortex and hypothalamus, lower levels in the liver and heart.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.
Caution	It is uncertain whether Met-1, Met-6 or Met-70 is the initiator.
Sequence caution	The sequence AAC14587.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway Traceable author statement Ref.11. Source: ProtInc elevation of cytosolic calcium ion concentration Traceable author statement Ref.11. Source: ProtInc feeding behavior Traceable author statement Ref.11. Source: ProtInc generation of precursor metabolites and energy Traceable author statement Ref.11. Source: ProtInc positive regulation of calcium ion transport Inferred from electronic annotation. Source: Compara
Cellular_component	integral to plasma membrane Traceable author statement Ref.11. Source: ProtInc nonmotile primary cilium Inferred from sequence or structural similarity. Source: BHF-UCL
Molecular_function	hormone binding Inferred from electronic annotation. Source: Compara melanin-concentrating hormone receptor activity Inferred from electronic annotation. Source: InterPro neuropeptide receptor activity Traceable author statement PubMed 10860944. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 422	422	Melanin-concentrating hormone receptor 1		PRO_0000069734
Regions
	Topological domain	1 – 113	113	Extracellular Potential
	Transmembrane	114 – 136	23	Helical; Name=1; Potential
	Topological domain	137 – 148	12	Cytoplasmic Potential
	Transmembrane	149 – 171	23	Helical; Name=2; Potential
	Topological domain	172 – 185	14	Extracellular Potential
	Transmembrane	186 – 208	23	Helical; Name=3; Potential
	Topological domain	209 – 227	19	Cytoplasmic Potential
	Transmembrane	228 – 250	23	Helical; Name=4; Potential
	Topological domain	251 – 278	28	Extracellular Potential
	Transmembrane	279 – 301	23	Helical; Name=5; Potential
	Topological domain	302 – 321	20	Cytoplasmic Potential
	Transmembrane	322 – 344	23	Helical; Name=6; Potential
	Topological domain	345 – 358	14	Extracellular Potential
	Transmembrane	359 – 381	23	Helical; Name=7; Potential
	Topological domain	382 – 422	41	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	82	1	N-linked (GlcNAc...) Potential
	Glycosylation	85	1	N-linked (GlcNAc...) Potential
	Glycosylation	92	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	25	1	T → M. Ref.2		VAR_026652
	Natural variant	28	1	D → V. Ref.2		VAR_026653
	Natural variant	32	1	N → D No significant functional differences. Ref.2 Ref.3 Ref.4 Ref.5 Ref.6 Ref.8 Ref.9 Corresponds to variant rs133072 [ dbSNP | Ensembl ].		VAR_016221
	Natural variant	34	1	G → R No changes in receptor binding or functional signaling. Ref.14		VAR_026654
	Natural variant	103	1	G → R. Corresponds to variant rs11914085 [ dbSNP | Ensembl ].		VAR_049417
	Natural variant	210	1	R → H. Ref.2		VAR_026655
	Natural variant	250	1	Y → H. Ref.13		VAR_026656
	Natural variant	305	1	T → M. Ref.2		VAR_026657
	Natural variant	317	1	R → Q. Ref.2 Ref.13 Corresponds to variant rs45439291 [ dbSNP | Ensembl ].		VAR_026658
	Natural variant	377	1	P → S. Ref.2		VAR_026659
	Natural variant	411	1	T → M. Ref.2		VAR_026660

Sequences

	Sequence		Length	Mass (Da)
	Q99705 [UniParc]. Last modified August 15, 2003. Version 2. Checksum: 3986919A18183818	FASTA	422	45,963
	10 20 30 40 50 60 MSVGAMKKGV GRAVGLGGGS GCQATEEDPL PNCGACAPGQ GGRRWRLPQP AWVEGSSARL 70 80 90 100 110 120 WEQATGTGWM DLEASLLPTG PNASNTSDGP DNLTSAGSPP RTGSISYINI IMPSVFGTIC 130 140 150 160 170 180 LLGIIGNSTV IFAVVKKSKL HWCNNVPDIF IINLSVVDLL FLLGMPFMIH QLMGNGVWHF 190 200 210 220 230 240 GETMCTLITA MDANSQFTST YILTAMAIDR YLATVHPISS TKFRKPSVAT LVICLLWALS 250 260 270 280 290 300 FISITPVWLY ARLIPFPGGA VGCGIRLPNP DTDLYWFTLY QFFLAFALPF VVITAAYVRI 310 320 330 340 350 360 LQRMTSSVAP ASQRSIRLRT KRVTRTAIAI CLVFFVCWAP YYVLQLTQLS ISRPTLTFVY 370 380 390 400 410 420 LYNAAISLGY ANSCLNPFVY IVLCETFRKR LVLSVKPAAQ GQLRAVSNAQ TADEERTESK GT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and identification of melanin-concentrating hormone as the endogenous ligand of the SLC-1 receptor." Shimomura Y., Mori M., Sugo T., Ishibashi Y., Abe M., Kurokawa T., Onda H., Nishimura O., Sumino Y., Fujino M. Biochem. Biophys. Res. Commun. 261:622-626(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Mutation analysis of the MCHR1 gene in human obesity." Wermter A.-K., Reichwald K., Buech T., Geller F., Platzer C., Huse K., Hess C., Remschmidt H., Gudermann T., Preibisch G., Siegfried W., Goldschmidt H.-P., Li W.-D., Price R.A., Biebermann H., Krude H., Vollmert C., Wichmann H.-E. , Illig T., Soerensen T.I.A., Astrup A., Larsen L.H., Pedersen O., Eberle D., Clement K., Blundell J., Wabitsch M., Schaefer H., Platzer M., Hinney A., Hebebrand J. Eur. J. Endocrinol. 152:851-862(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS MET-25; VAL-28; ASP-32; HIS-210; MET-305; GLN-317; SER-377 AND MET-411, CHARACTERIZATION OF VARIANT ASP-32.
[3]	"A genome annotation-driven approach to cloning the human ORFeome." Collins J.E., Wright C.L., Edwards C.A., Davis M.P., Grinham J.A., Cole C.G., Goward M.E., Aguado B., Mallya M., Mokrab Y., Huckle E.J., Beare D.M., Dunham I. Genome Biol. 5:R84.1-R84.11(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ASP-32.
[4]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ASP-32.
[5]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." King M.M., Aronstam R.S., Sharma S.V. Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ASP-32. Tissue: Brain.
[6]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ASP-32. Tissue: Brain.
[7]	"The DNA sequence of human chromosome 22." Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M., Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C., Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E., Bridgeman A.M. , Buck D., Burgess J., Burrill W.D., Burton J., Carder C., Carter N.P., Chen Y., Clark G., Clegg S.M., Cobley V.E., Cole C.G., Collier R.E., Connor R., Conroy D., Corby N.R., Coville G.J., Cox A.V., Davis J., Dawson E., Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M., Ellington A.G., Evans K.L., Fey J.M., Fleming K., French L., Garner A.A., Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C., Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S., Hunt S.E., Jones M.C., Kershaw J., Kimberley A.M., King A., Laird G.K., Langford C.F., Leversha M.A., Lloyd C., Lloyd D.M., Martyn I.D., Mashreghi-Mohammadi M., Matthews L.H., Mccann O.T., Mcclay J., Mclaren S., McMurray A.A., Milne S.A., Mortimore B.J., Odell C.N., Pavitt R., Pearce A.V., Pearson D., Phillimore B.J.C.T., Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y., Rogers L., Ross M.T., Scott C.E., Sehra H.K., Skuce C.D., Smalley S., Smith M.L., Soderlund C., Spragon L., Steward C.A., Sulston J.E., Swann R.M., Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L., Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L., Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N., Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J., Shintani A., Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S., Roe B.A., Chen F., Chu L., Crabtree J., Deschamps S., Do A., Do T., Dorman A., Fang F., Fu Y., Hu P., Hua A., Kenton S., Lai H., Lao H.I., Lewis J., Lewis S., Lin S.-P., Loh P., Malaj E., Nguyen T., Pan H., Phan S., Qi S., Qian Y., Ray L., Ren Q., Shaull S., Sloan D., Song L., Wang Q., Wang Y., Wang Z., White J., Willingham D., Wu H., Yao Z., Zhan M., Zhang G., Chissoe S., Murray J., Miller N., Minx P., Fulton R., Johnson D., Bemis G., Bentley D., Bradshaw H., Bourne S., Cordes M., Du Z., Fulton L., Goela D., Graves T., Hawkins J., Hinds K., Kemp K., Latreille P., Layman D., Ozersky P., Rohlfing T., Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K., Nelson J., Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R., Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S., Budarf M.L., McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E., Edelmann L., Kim U.J., Shizuya H., Simon M.I., Dumanski J.P., Peyrard M., Kedra D., Seroussi E., Fransson I., Tapia I., Bruder C.E., O'Brien K.P., Wilkinson P., Bodenteich A., Hartman K., Hu X., Khan A.S., Lane L., Tilahun Y., Wright H. Nature 402:489-495(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ASP-32.
[9]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ASP-32. Tissue: Brain.
[10]	"Characterization of a human gene related to genes encoding somatostatin receptors." Kolakowski L.F. Jr., Jung B.P., Nguyen T., Johnson M.P., Lynch K.R., Cheng R., Heng H.H.Q., George S.R., O'Dowd B.F. FEBS Lett. 398:253-258(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 77-422.
[11]	"Melanin-concentrating hormone is the cognate ligand for the orphan G-protein-coupled receptor SLC-1." Chambers J., Ames R.S., Bergsma D., Muir A., Fitzgerald L.R., Hervieu G., Dytko G.M., Foley J.J., Martin J., Liu W.S., Park J., Ellis C., Ganguly S., Konchar S., Cluderay J., Leslie R., Wilson S., Sarau H.M. Nature 400:261-265(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[12]	"Interaction of neurochondrin with the melanin-concentrating hormone receptor 1 interferes with G protein-coupled signal transduction but not agonist-mediated internalization." Francke F., Ward R.J., Jenkins L., Kellett E., Richter D., Milligan G., Baechner D. J. Biol. Chem. 281:32496-32507(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH NCDN.
[13]	"Melanin-concentrating hormone receptor mutations and human obesity: functional analysis." Gibson W.T., Pissios P., Trombly D.J., Luan J., Keogh J., Wareham N.J., Maratos-Flier E., O'Rahilly S., Farooqi I.S. Obes. Res. 12:743-749(2004) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANTS HIS-250 AND GLN-317, CHARACTERIZATION OF VARIANT GLN-317.
[14]	"Identification and characterization of single-nucleotide polymorphisms in MCH-R1 and MCH-R2." Hawes B.E., Green B., O'Neill K., Fried S., Arreaza M.G., Qiu P., Simon J.S. Obes. Res. 12:1327-1334(2004) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT ARG-34, CHARACTERIZATION OF VARIANT ARG-34.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB063174 mRNA. Translation: BAB60890.1. AF490537 mRNA. Translation: AAO14670.1. CR456497 mRNA. Translation: CAG30383.1. BT006725 mRNA. Translation: AAP35371.1. AY562945 mRNA. Translation: AAS72373.1. AK314857 mRNA. Translation: BAG37373.1. Z86090 Genomic DNA. Translation: CAI17933.1. CH471095 Genomic DNA. Translation: EAW60388.1. BC001736 mRNA. Translation: AAH01736.1. BC021146 mRNA. Translation: AAH21146.1. U71092 Genomic DNA. Translation: AAC14587.1. Sequence problems.
IPI	IPI00017705.
PIR	JC7080.
RefSeq	NP_005288.3. NM_005297.3.
UniGene	Hs.248122.
3D structure databases
ProteinModelPortal	Q99705.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000249016.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	Q99705.
Polymorphism databases
DMDM	33860175.
Proteomic databases
PaxDb	Q99705.
PRIDE	Q99705.
Protocols and materials databases
DNASU	2847.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000249016; ENSP00000249016; ENSG00000128285.
GeneID	2847.
KEGG	hsa:2847.
UCSC	uc003ayz.3. human.
Organism-specific databases
CTD	2847.
GeneCards	GC22P041074.
HGNC	HGNC:4479. MCHR1.
HPA	HPA004149.
MIM	601751. gene.
neXtProt	NX_Q99705.
PharmGKB	PA28867.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG282817.
HOGENOM	HOG000000704.
HOVERGEN	HBG105726.
InParanoid	Q99705.
KO	K04320.
OMA	MGNGVWH.
OrthoDB	EOG43XV3F.
PhylomeDB	Q99705.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
ArrayExpress	Q99705.
Bgee	Q99705.
CleanEx	HS_MCHR1.
Genevestigator	Q99705.
GermOnline	ENSG00000128285. Homo sapiens.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR004047. MCH1_rcpt. IPR008361. MCH_rcpt. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR01507. MCH1RECEPTOR. PR01783. MCHRECEPTOR.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. False negative. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q99705.
ChEMBL	CHEMBL344.
GenomeRNAi	2847.
NextBio	11231.
SOURCE	Search...

Entry information

Entry name

MCHR1_HUMAN

Accession

Primary (citable) accession number: Q99705
Secondary accession number(s): B2RBX6 , Q5R3J1, Q96S47, Q9BV08

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	August 15, 2003
Last modified:	May 1, 2013
This is version 118 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.