Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q99689

- FEZ1_HUMAN

UniProt

Q99689 - FEZ1_HUMAN

Protein

Fasciculation and elongation protein zeta-1

Gene

FEZ1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    May be involved in axonal outgrowth as component of the network of molecules that regulate cellular morphology and axon guidance machinery. Able to restore partial locomotion and axonal fasciculation to C.elegans unc-76 mutants in germline transformation experiments. May participate in the transport of mitochondria and other cargos along microtubules.2 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. axon guidance Source: ProtInc
    2. cell adhesion Source: ProtInc
    3. cellular response to growth factor stimulus Source: Ensembl
    4. establishment of mitochondrion localization Source: Ensembl
    5. nervous system development Source: ProtInc
    6. positive regulation of neuron differentiation Source: Ensembl
    7. positive regulation of neuron projection development Source: Ensembl
    8. transport Source: UniProtKB-KW

    Keywords - Biological processi

    Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fasciculation and elongation protein zeta-1
    Alternative name(s):
    Zygin I
    Zygin-1
    Gene namesi
    Name:FEZ1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:3659. FEZ1.

    Subcellular locationi

    Cytoplasmcytoskeletonmicrotubule organizing centercentrosome 1 Publication. Cell membrane By similarity
    Note: Colocalizes with both, alpha- and gamma-tubulin. Translocated from the plasma membrane to the cytoplasm by activation of the PKC zeta By similarity.By similarity

    GO - Cellular componenti

    1. axon Source: Ensembl
    2. centrosome Source: Ensembl
    3. cytoplasm Source: HPA
    4. dendrite Source: Ensembl
    5. microtubule Source: UniProtKB-KW
    6. microtubule cytoskeleton Source: HPA
    7. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Microtubule

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi248 – 2481E → A: No effect on SCOC--binding. 1 Publication
    Mutagenesisi254 – 2541L → P: Loss of SCOC-binding. No effect on ULK1-binding. 1 Publication
    Mutagenesisi260 – 2601L → P: Loss of SCOC-binding. No effect on ULK1-binding. 1 Publication
    Mutagenesisi264 – 2641K → A: No effect on SCOC-binding. 1 Publication

    Organism-specific databases

    PharmGKBiPA28100.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 392392Fasciculation and elongation protein zeta-1PRO_0000189525Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei58 – 581PhosphoserineBy similarity
    Disulfide bondi133 – 133Interchain1 Publication

    Post-translational modificationi

    Phosphorylated by protein kinase C zeta; which enhances interaction with UBE4B and polyubiquitination.1 Publication
    Polyubiquitinated in a UBE4B-dependent manner; which does not lead to proteasomal degradation and may be important for neurogenic activity. Polyubiquitin linkage seems to be mainly through Lys-26.1 Publication

    Keywords - PTMi

    Disulfide bond, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PaxDbiQ99689.
    PRIDEiQ99689.

    PTM databases

    PhosphoSiteiQ99689.

    Expressioni

    Tissue specificityi

    Mainly expressed in brain.

    Gene expression databases

    ArrayExpressiQ99689.
    BgeeiQ99689.
    CleanExiHS_FEZ1.
    GenevestigatoriQ99689.

    Organism-specific databases

    HPAiHPA038490.

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked. May form heterodimers with FEZ2. Interacts with the NH2-terminal variable region (V1) of PKC zeta and weakly with that of PKC epsilon By similarity. Interacts with UBE4B. Interacts with SAP30L. Interacts with SCOC and ULK1; SCOC interferes with ULK1-binding to FEZ1. Directly interacts with SCOC and UVRAG. Stabilizes the interaction between SCOC and UVRAG during amino acid starvation.By similarity4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    HTTP428585EBI-396435,EBI-466029
    KIAA1377Q9P2H02EBI-396435,EBI-473176
    NEK1Q96PY62EBI-396435,EBI-373615
    TXNDC9O145302EBI-396435,EBI-707554

    Protein-protein interaction databases

    BioGridi114997. 56 interactions.
    DIPiDIP-32494N.
    IntActiQ99689. 38 interactions.
    MINTiMINT-1403506.
    STRINGi9606.ENSP00000278919.

    Structurei

    3D structure databases

    ProteinModelPortaliQ99689.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili230 – 29869Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi95 – 984Poly-Glu
    Compositional biasi140 – 1456Poly-Glu
    Compositional biasi182 – 1865Poly-Glu

    Sequence similaritiesi

    Belongs to the zygin family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG273183.
    HOGENOMiHOG000019494.
    HOVERGENiHBG005657.
    OMAiEMMENSP.
    OrthoDBiEOG7CCBRQ.
    PhylomeDBiQ99689.
    TreeFamiTF313128.

    Family and domain databases

    InterProiIPR011680. FEZ.
    IPR015642. FEZ_1.
    [Graphical view]
    PANTHERiPTHR12394. PTHR12394. 1 hit.
    PTHR12394:SF4. PTHR12394:SF4. 1 hit.
    PfamiPF07763. FEZ. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Long (identifier: Q99689-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEAPLVSLDE EFEDLRPSCS EDPEEKPQCF YGSSPHHLED PSLSELENFS    50
    SEIISFKSME DLVNEFDEKL NVCFRNYNAK TENLAPVKNQ LQIQEEEETL 100
    QDEEVWDALT DNYIPSLSED WRDPNIEALN GNCSDTEIHE KEEEEFNEKS 150
    ENDSGINEEP LLTADQVIEE IEEMMQNSPD PEEEEEVLEE EDGGETSSQA 200
    DSVLLQEMQA LTQTFNNNWS YEGLRHMSGS ELTELLDQVE GAIRDFSEEL 250
    VQQLARRDEL EFEKEVKNSF ITVLIEVQNK QKEQRELMKK RRKEKGLSLQ 300
    SSRIEKGNQM PLKRFSMEGI SNILQSGIRQ TFGSSGTDKQ YLNTVIPYEK 350
    KASPPSVEDL QMLTNILFAM KEDNEKVPTL LTDYILKVLC PT 392
    Length:392
    Mass (Da):45,119
    Last modified:May 1, 1997 - v1
    Checksum:i4226ED5EA17706F2
    GO
    Isoform Short (identifier: Q99689-2) [UniParc]FASTAAdd to Basket

    Also known as: FEZ1-T

    The sequence of this isoform differs from the canonical sequence as follows:
         105-392: Missing.

    Show »
    Length:104
    Mass (Da):12,074
    Checksum:iA614EFAFF872EAF8
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti123 – 1231D → E.
    Corresponds to variant rs597570 [ dbSNP | Ensembl ].
    VAR_020461

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei105 – 392288Missing in isoform Short. 1 PublicationVSP_006951Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U60060 mRNA. Translation: AAC51282.1.
    U60062 mRNA. Translation: AAC51284.1.
    U69139 mRNA. Translation: AAB40660.1.
    CR456817 mRNA. Translation: CAG33098.1.
    CR541954 mRNA. Translation: CAG46752.1.
    BC009545 mRNA. Translation: AAH09545.1.
    CCDSiCCDS31716.1. [Q99689-1]
    CCDS44758.1. [Q99689-2]
    RefSeqiNP_005094.1. NM_005103.4. [Q99689-1]
    NP_072043.1. NM_022549.3. [Q99689-2]
    XP_005271791.1. XM_005271734.1. [Q99689-1]
    XP_005271792.1. XM_005271735.1. [Q99689-1]
    UniGeneiHs.224008.
    Hs.732083.

    Genome annotation databases

    EnsembliENST00000278919; ENSP00000278919; ENSG00000149557. [Q99689-1]
    ENST00000366139; ENSP00000393425; ENSG00000149557. [Q99689-2]
    ENST00000524435; ENSP00000431521; ENSG00000149557. [Q99689-2]
    GeneIDi9638.
    KEGGihsa:9638.
    UCSCiuc001qbx.3. human. [Q99689-1]
    uc001qby.2. human. [Q99689-2]

    Polymorphism databases

    DMDMi13431526.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U60060 mRNA. Translation: AAC51282.1 .
    U60062 mRNA. Translation: AAC51284.1 .
    U69139 mRNA. Translation: AAB40660.1 .
    CR456817 mRNA. Translation: CAG33098.1 .
    CR541954 mRNA. Translation: CAG46752.1 .
    BC009545 mRNA. Translation: AAH09545.1 .
    CCDSi CCDS31716.1. [Q99689-1 ]
    CCDS44758.1. [Q99689-2 ]
    RefSeqi NP_005094.1. NM_005103.4. [Q99689-1 ]
    NP_072043.1. NM_022549.3. [Q99689-2 ]
    XP_005271791.1. XM_005271734.1. [Q99689-1 ]
    XP_005271792.1. XM_005271735.1. [Q99689-1 ]
    UniGenei Hs.224008.
    Hs.732083.

    3D structure databases

    ProteinModelPortali Q99689.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114997. 56 interactions.
    DIPi DIP-32494N.
    IntActi Q99689. 38 interactions.
    MINTi MINT-1403506.
    STRINGi 9606.ENSP00000278919.

    PTM databases

    PhosphoSitei Q99689.

    Polymorphism databases

    DMDMi 13431526.

    Proteomic databases

    PaxDbi Q99689.
    PRIDEi Q99689.

    Protocols and materials databases

    DNASUi 9638.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000278919 ; ENSP00000278919 ; ENSG00000149557 . [Q99689-1 ]
    ENST00000366139 ; ENSP00000393425 ; ENSG00000149557 . [Q99689-2 ]
    ENST00000524435 ; ENSP00000431521 ; ENSG00000149557 . [Q99689-2 ]
    GeneIDi 9638.
    KEGGi hsa:9638.
    UCSCi uc001qbx.3. human. [Q99689-1 ]
    uc001qby.2. human. [Q99689-2 ]

    Organism-specific databases

    CTDi 9638.
    GeneCardsi GC11M125349.
    HGNCi HGNC:3659. FEZ1.
    HPAi HPA038490.
    MIMi 604825. gene.
    neXtProti NX_Q99689.
    PharmGKBi PA28100.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG273183.
    HOGENOMi HOG000019494.
    HOVERGENi HBG005657.
    OMAi EMMENSP.
    OrthoDBi EOG7CCBRQ.
    PhylomeDBi Q99689.
    TreeFami TF313128.

    Miscellaneous databases

    ChiTaRSi FEZ1. human.
    GeneWikii FEZ1.
    GenomeRNAii 9638.
    NextBioi 36177.
    PROi Q99689.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q99689.
    Bgeei Q99689.
    CleanExi HS_FEZ1.
    Genevestigatori Q99689.

    Family and domain databases

    InterProi IPR011680. FEZ.
    IPR015642. FEZ_1.
    [Graphical view ]
    PANTHERi PTHR12394. PTHR12394. 1 hit.
    PTHR12394:SF4. PTHR12394:SF4. 1 hit.
    Pfami PF07763. FEZ. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The Caenorhabditis elegans gene unc-76 and its human homologs define a new gene family involved in axonal outgrowth and fasciculation."
      Bloom L., Horvitz H.R.
      Proc. Natl. Acad. Sci. U.S.A. 94:3414-3419(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
    2. "Zigins: a family of synaptotagmin-interacting proteins related to unc-76."
      Sugita S., von Poser C., Rosahl T.W., Hata Y., Suedhof T.C.
      Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
    3. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
    4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
      Tissue: Pancreas.
    6. "Functional regulation of FEZ1 by the U-box-type ubiquitin ligase E4B contributes to neuritogenesis."
      Okumura F., Hatakeyama S., Matsumoto M., Kamura T., Nakayama K.
      J. Biol. Chem. 279:53533-53543(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH UBE4B, PHOSPHORYLATION, UBIQUITINATION.
    7. "FEZ1 dimerization and interaction with transcription regulatory proteins involves its coiled-coil region."
      Assmann E.M., Alborghetti M.R., Camargo M.E.R., Kobarg J.
      J. Biol. Chem. 281:9869-9881(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, INTERACTION WITH SAP30L.
    8. "Over-expression of GFP-FEZ1 causes generation of multi-lobulated nuclei mediated by microtubules in HEK293 cells."
      Lanza D.C., Trindade D.M., Assmann E.M., Kobarg J.
      Exp. Cell Res. 314:2028-2039(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    9. "Human FEZ1 protein forms a disulfide bond mediated dimer: implications for cargo transport."
      Alborghetti M.R., Furlan A.S., Silva J.C., Paes Leme A.F., Torriani I.C., Kobarg J.
      J. Proteome Res. 9:4595-4603(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, INTERACTION WITH SCOC, DISULFIDE BOND.
    10. "Genome-wide siRNA screen reveals amino acid starvation-induced autophagy requires SCOC and WAC."
      McKnight N.C., Jefferies H.B., Alemu E.A., Saunders R.E., Howell M., Johansen T., Tooze S.A.
      EMBO J. 31:1931-1946(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH SCOC; ULK1 AND UVRAG, MUTAGENESIS OF GLU-248; LEU-254; LEU-260 AND LYS-264.

    Entry informationi

    Entry nameiFEZ1_HUMAN
    AccessioniPrimary (citable) accession number: Q99689
    Secondary accession number(s): O00679, O00728, Q6IBI7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 27, 2001
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 131 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3