ID   GPR21_HUMAN             Reviewed;         349 AA.
AC   Q99679; B2R8W9; Q6NXU2;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   27-MAR-2024, entry version 168.
DE   RecName: Full=Probable G-protein coupled receptor 21;
GN   Name=GPR21;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9073069; DOI=10.1016/s0378-1119(96)00722-6;
RA   O'Dowd B.F., Nguyen T., Jung B.P., Marchese A., Cheng R., Heng H.H.Q.,
RA   Kolakowski L.F. Jr., Lynch K.R., George S.R.;
RT   "Cloning and chromosomal mapping of four putative novel human G-protein-
RT   coupled receptor genes.";
RL   Gene 187:75-81(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Orphan receptor.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC   -!- TISSUE SPECIFICITY: Not detected in the brain regions thalamus,
CC       putamen, caudate, frontal cortex, pons, hypothalamus, hippocampus.
CC   -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR   EMBL; U66580; AAC51303.1; -; Genomic_DNA.
DR   EMBL; AK313539; BAG36316.1; -; mRNA.
DR   EMBL; CH471090; EAW87559.1; -; Genomic_DNA.
DR   EMBL; BC066885; AAH66885.2; -; mRNA.
DR   EMBL; BC066886; AAH66886.2; -; mRNA.
DR   EMBL; BC067532; AAH67532.2; -; mRNA.
DR   EMBL; BC067533; AAH67533.2; -; mRNA.
DR   CCDS; CCDS6849.1; -.
DR   RefSeq; NP_005285.1; NM_005294.2.
DR   RefSeq; XP_005251990.1; XM_005251933.3.
DR   PDB; 8HIX; EM; 3.12 A; R=1-327.
DR   PDB; 8HJ0; EM; 3.12 A; R=1-327.
DR   PDB; 8HJ2; EM; 3.80 A; R=1-349.
DR   PDB; 8HMV; EM; 2.91 A; A=30-318.
DR   PDBsum; 8HIX; -.
DR   PDBsum; 8HJ0; -.
DR   PDBsum; 8HJ2; -.
DR   PDBsum; 8HMV; -.
DR   AlphaFoldDB; Q99679; -.
DR   EMDB; EMD-33480; -.
DR   EMDB; EMD-33481; -.
DR   EMDB; EMD-33482; -.
DR   EMDB; EMD-34903; -.
DR   SMR; Q99679; -.
DR   BioGRID; 109103; 82.
DR   IntAct; Q99679; 73.
DR   STRING; 9606.ENSP00000362746; -.
DR   ChEMBL; CHEMBL4523913; -.
DR   GlyCosmos; Q99679; 2 sites, No reported glycans.
DR   GlyGen; Q99679; 2 sites.
DR   iPTMnet; Q99679; -.
DR   PhosphoSitePlus; Q99679; -.
DR   BioMuta; GPR21; -.
DR   DMDM; 2495040; -.
DR   PaxDb; 9606-ENSP00000362746; -.
DR   PeptideAtlas; Q99679; -.
DR   ProteomicsDB; 78393; -.
DR   Antibodypedia; 30412; 108 antibodies from 17 providers.
DR   DNASU; 2844; -.
DR   Ensembl; ENST00000616002.3; ENSP00000482287.1; ENSG00000188394.8.
DR   GeneID; 2844; -.
DR   KEGG; hsa:2844; -.
DR   MANE-Select; ENST00000616002.3; ENSP00000482287.1; NM_005294.3; NP_005285.1.
DR   UCSC; uc011lzk.3; human.
DR   AGR; HGNC:4476; -.
DR   CTD; 2844; -.
DR   DisGeNET; 2844; -.
DR   GeneCards; GPR21; -.
DR   HGNC; HGNC:4476; GPR21.
DR   HPA; ENSG00000188394; Tissue enhanced (brain).
DR   MIM; 601909; gene.
DR   neXtProt; NX_Q99679; -.
DR   OpenTargets; ENSG00000188394; -.
DR   PharmGKB; PA28864; -.
DR   VEuPathDB; HostDB:ENSG00000188394; -.
DR   eggNOG; KOG3656; Eukaryota.
DR   GeneTree; ENSGT00940000163543; -.
DR   HOGENOM; CLU_009579_3_3_1; -.
DR   InParanoid; Q99679; -.
DR   OMA; WCADSWK; -.
DR   OrthoDB; 5400179at2759; -.
DR   PhylomeDB; Q99679; -.
DR   TreeFam; TF332372; -.
DR   PathwayCommons; Q99679; -.
DR   SignaLink; Q99679; -.
DR   BioGRID-ORCS; 2844; 8 hits in 1134 CRISPR screens.
DR   GeneWiki; GPR21; -.
DR   GenomeRNAi; 2844; -.
DR   Pharos; Q99679; Tdark.
DR   PRO; PR:Q99679; -.
DR   Proteomes; UP000005640; Chromosome 9.
DR   RNAct; Q99679; Protein.
DR   Bgee; ENSG00000188394; Expressed in male germ line stem cell (sensu Vertebrata) in testis and 111 other cell types or tissues.
DR   ExpressionAtlas; Q99679; baseline and differential.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0004930; F:G protein-coupled receptor activity; IEA:UniProtKB-KW.
DR   GO; GO:0042593; P:glucose homeostasis; IEA:Ensembl.
DR   GO; GO:0008286; P:insulin receptor signaling pathway; IEA:Ensembl.
DR   GO; GO:0046627; P:negative regulation of insulin receptor signaling pathway; IEA:Ensembl.
DR   GO; GO:0040018; P:positive regulation of multicellular organism growth; IEA:Ensembl.
DR   CDD; cd00637; 7tm_classA_rhodopsin-like; 1.
DR   Gene3D; 1.20.1070.10; Rhodopsin 7-helix transmembrane proteins; 1.
DR   InterPro; IPR000276; GPCR_Rhodpsn.
DR   InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR   PANTHER; PTHR24249:SF384; G-PROTEIN COUPLED RECEPTOR 21-RELATED; 1.
DR   PANTHER; PTHR24249; HISTAMINE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR; 1.
DR   Pfam; PF00001; 7tm_1; 1.
DR   PRINTS; PR00237; GPCRRHODOPSN.
DR   SUPFAM; SSF81321; Family A G protein-coupled receptor-like; 1.
DR   PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR   PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
DR   Genevisible; Q99679; HS.
PE   1: Evidence at protein level;
KW   3D-structure; Cell membrane; G-protein coupled receptor; Glycoprotein;
KW   Membrane; Receptor; Reference proteome; Transducer; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..349
FT                   /note="Probable G-protein coupled receptor 21"
FT                   /id="PRO_0000069542"
FT   TOPO_DOM        1..32
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        33..53
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        54..75
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        76..96
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        97..104
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        105..125
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        126..147
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        148..168
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        169..191
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        192..212
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        213..252
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        253..273
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        274..283
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        284..304
FT                   /note="Helical; Name=7"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        305..349
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        2
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        8
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   HELIX           26..55
FT                   /evidence="ECO:0007829|PDB:8HIX"
FT   STRAND          57..61
FT                   /evidence="ECO:0007829|PDB:8HIX"
FT   HELIX           65..90
FT                   /evidence="ECO:0007829|PDB:8HIX"
FT   STRAND          96..99
FT                   /evidence="ECO:0007829|PDB:8HIX"
FT   HELIX           100..132
FT                   /evidence="ECO:0007829|PDB:8HIX"
FT   STRAND          133..136
FT                   /evidence="ECO:0007829|PDB:8HIX"
FT   HELIX           137..140
FT                   /evidence="ECO:0007829|PDB:8HIX"
FT   HELIX           143..160
FT                   /evidence="ECO:0007829|PDB:8HIX"
FT   HELIX           162..166
FT                   /evidence="ECO:0007829|PDB:8HIX"
FT   TURN            174..177
FT                   /evidence="ECO:0007829|PDB:8HIX"
FT   HELIX           179..184
FT                   /evidence="ECO:0007829|PDB:8HIX"
FT   HELIX           189..198
FT                   /evidence="ECO:0007829|PDB:8HIX"
FT   HELIX           200..231
FT                   /evidence="ECO:0007829|PDB:8HIX"
FT   HELIX           253..277
FT                   /evidence="ECO:0007829|PDB:8HIX"
FT   HELIX           283..294
FT                   /evidence="ECO:0007829|PDB:8HIX"
FT   HELIX           296..307
FT                   /evidence="ECO:0007829|PDB:8HIX"
FT   HELIX           310..323
FT                   /evidence="ECO:0007829|PDB:8HIX"
SQ   SEQUENCE   349 AA;  39515 MW;  5369DFC216E9EEC4 CRC64;
     MNSTLDGNQS SHPFCLLAFG YLETVNFCLL EVLIIVFLTV LIISGNIIVI FVFHCAPLLN
     HHTTSYFIQT MAYADLFVGV SCVVPSLSLL HHPLPVEESL TCQIFGFVVS VLKSVSMASL
     ACISIDRYIA ITKPLTYNTL VTPWRLRLCI FLIWLYSTLV FLPSFFHWGK PGYHGDVFQW
     CAESWHTDSY FTLFIVMMLY APAALIVCFT YFNIFRICQQ HTKDISERQA RFSSQSGETG
     EVQACPDKRY AMVLFRITSV FYILWLPYII YFLLESSTGH SNRFASFLTT WLAISNSFCN
     CVIYSLSNSV FQRGLKRLSG AMCTSCASQT TANDPYTVRS KGPLNGCHI
//