Q99677 (LPAR4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lysophosphatidic acid receptor 4
Short name=LPA receptor 4
Short name=LPA-4

Alternative name(s):
G-protein coupled receptor 23
P2Y purinoceptor 9
Short name=P2Y9
P2Y5-like receptor
Purinergic receptor 9

Gene names

Name:	LPAR4
Synonyms:	GPR23, LPA4, P2RY9

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	370 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Transduces a signal by increasing the intracellular calcium ions and by stimulating adenylyl cyclase activity. The rank order of potency for agonists of this receptor is 1-oleoyl- > 1-stearoyl- > 1-palmitoyl- > 1-myristoyl- > 1-alkyl- > 1-alkenyl-LPA. Ref.9
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	High expression in ovary. Not detected in the brain regions thalamus, putamen, caudate, frontal cortex, pons, hypothalamus and hippocampus. Ref.9
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Lipid-binding
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc
Molecular_function	G-protein coupled purinergic nucleotide receptor activity Inferred from electronic annotation. Source: InterPro G-protein coupled receptor activity Traceable author statement Ref.1. Source: ProtInc lipid binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 370	370	Lysophosphatidic acid receptor 4		PRO_0000070033
Regions
	Topological domain	1 – 43	43	Extracellular Potential
	Transmembrane	44 – 64	21	Helical; Name=1; Potential
	Topological domain	65 – 73	9	Cytoplasmic Potential
	Transmembrane	74 – 94	21	Helical; Name=2; Potential
	Topological domain	95 – 112	18	Extracellular Potential
	Transmembrane	113 – 133	21	Helical; Name=3; Potential
	Topological domain	134 – 155	22	Cytoplasmic Potential
	Transmembrane	156 – 176	21	Helical; Name=4; Potential
	Topological domain	177 – 203	27	Extracellular Potential
	Transmembrane	204 – 224	21	Helical; Name=5; Potential
	Topological domain	225 – 254	30	Cytoplasmic Potential
	Transmembrane	255 – 275	21	Helical; Name=6; Potential
	Topological domain	276 – 294	19	Extracellular Potential
	Transmembrane	295 – 315	21	Helical; Name=7; Potential
	Topological domain	316 – 370	55	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	15	1	N-linked (GlcNAc...) Potential
	Glycosylation	24	1	N-linked (GlcNAc...) Potential
	Glycosylation	28	1	N-linked (GlcNAc...) Potential
	Glycosylation	183	1	N-linked (GlcNAc...) Ref.10
	Disulfide bond	111 ↔ 188		By similarity
Experimental info
	Sequence conflict	49	1	V → A in AAH69996. Ref.8
	Sequence conflict	192	1	F → L in AAB66322. Ref.3
	Sequence conflict	240	1	I → V in AAH95538. Ref.8

Sequences

	Sequence		Length	Mass (Da)
	Q99677 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 20857F52A3929E48	FASTA	370	41,895
	10 20 30 40 50 60 MGDRRFIDFQ FQDSNSSLRP RLGNATANNT CIVDDSFKYN LNGAVYSVVF ILGLITNSVS 70 80 90 100 110 120 LFVFCFRMKM RSETAIFITN LAVSDLLFVC TLPFKIFYNF NRHWPFGDTL CKISGTAFLT 130 140 150 160 170 180 NIYGSMLFLT CISVDRFLAI VYPFRSRTIR TRRNSAIVCA GVWILVLSGG ISASLFSTTN 190 200 210 220 230 240 VNNATTTCFE GFSKRVWKTY LSKITIFIEV VGFIIPLILN VSCSSVVLRT LRKPATLSQI 250 260 270 280 290 300 GTNKKKVLKM ITVHMAVFVV CFVPYNSVLF LYALVRSQAI TNCFLERFAK IMYPITLCLA 310 320 330 340 350 360 TLNCCFDPFI YYFTLESFQK SFYINAHIRM ESLFKTETPL TTKPSLPAIQ EEVSDQTTNN 370 GGELMLESTF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and chromosomal mapping of four putative novel human G-protein-coupled receptor genes." O'Dowd B.F., Nguyen T., Jung B.P., Marchese A., Cheng R., Heng H.H.Q., Kolakowski L.F. Jr., Lynch K.R., George S.R. Gene 187:75-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Cloning, characterization and chromosomal localization of a novel human purinergic receptor (P2Y9)." Bohm S.K., Khitin L.M., Payan D.P., Bunnett N.W. Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[3]	"Cloning of a human heptahelical receptor closely related to the P2Y5 receptor." Janssens R., Boeynaems J.-M., Godart M., Communi D. Biochem. Biophys. Res. Commun. 236:106-112(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Kopatz S.A., Aronstam R.S., Sharma S.V. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Thalamus.
[6]	"The DNA sequence of the human X chromosome." Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C. , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R. Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[9]	"Identification of p2y9/GPR23 as a novel G protein-coupled receptor for lysophosphatidic acid, structurally distant from the Edg family." Noguchi K., Ishii S., Shimizu T. J. Biol. Chem. 278:25600-25606(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[10]	"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry." Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D. J. Proteome Res. 4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-183, MASS SPECTROMETRY. Tissue: Plasma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U66578 Genomic DNA. Translation: AAC51301.1. U90323 Genomic DNA. Translation: AAB62087.1. U90322 mRNA. Translation: AAB62088.1. AF005419 Genomic DNA. Translation: AAB66322.1. AY301274 Genomic DNA. Translation: AAP58404.1. AK314134 mRNA. Translation: BAG36824.1. AL590083 Genomic DNA. Translation: CAD18851.1. CH471104 Genomic DNA. Translation: EAW98596.1. BC069996 mRNA. Translation: AAH69996.1. BC074722 mRNA. Translation: AAH74722.1. BC095538 mRNA. Translation: AAH95538.1.
IPI	IPI00017041.
PIR	JC5549.
RefSeq	NP_005287.1. NM_005296.2.
UniGene	Hs.522701.
3D structure databases
ProteinModelPortal	Q99677.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q99677. 1 interaction.
STRING	9606.ENSP00000362398.
Protein family/group databases
GPCRDB	Search...
Polymorphism databases
DMDM	2495042.
Proteomic databases
PaxDb	Q99677.
PRIDE	Q99677.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000373301; ENSP00000362398; ENSG00000147145. ENST00000435339; ENSP00000408205; ENSG00000147145.
GeneID	2846.
KEGG	hsa:2846.
UCSC	uc010nme.3. human.
Organism-specific databases
CTD	2846.
GeneCards	GC0XP078004.
HGNC	HGNC:4478. LPAR4.
MIM	300086. gene.
neXtProt	NX_Q99677.
PharmGKB	PA162394222.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG147814.
HOGENOM	HOG000231307.
HOVERGEN	HBG101917.
InParanoid	Q99677.
KO	K04275.
OMA	QAITNCL.
OrthoDB	EOG4RFKSQ.
PhylomeDB	Q99677.
Enzyme and pathway databases
Pathway_Interaction_DB	lysophospholipid_pathway. LPA receptor mediated events. lpa4_pathway. LPA4-mediated signaling events.
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
Bgee	Q99677.
CleanEx	HS_LPAR4.
Genevestigator	Q99677.
GermOnline	ENSG00000147145. Homo sapiens.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR002188. P2Y5_purnocptor. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR01067. P2Y5ORPHANR.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL5968.
GenomeRNAi	2846.
NextBio	11227.
SOURCE	Search...

Entry information

Entry name

LPAR4_HUMAN

Accession

Primary (citable) accession number: Q99677
Secondary accession number(s): B2RAC7 , O15132, Q502U9, Q6NSP5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 1, 1997
Last modified:	May 1, 2013
This is version 114 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.