Q99653 (CHP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Calcium-binding protein p22

Alternative name(s):
Calcineurin B homolog
Calcineurin homologous protein
Calcium-binding protein CHP

Gene names

Name:

CHP

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	195 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for constitutive membrane traffic. Inhibits GTPase-stimulated Na+/H+ exchange. Also inhibits calcineurin phosphatase activity. Required for activity of SLC9A1/NHE1. Ref.7
Subunit structure	Monomer By similarity. Specifically binds to SLC9A1/NHE1 at a domain that is critical for growth factor stimulation of exchange activity.
Subcellular location	Cytoplasm By similarity.
Tissue specificity	Ubiquitously expressed. Has been found in fetal eye, lung, liver, muscle, heart, kidney, thymus and spleen.
Post-translational modification	Both N-myristoylation and calcium-mediated conformational changes are essential for its function in exocytic traffic By similarity. Phosphorylated; decreased phosphorylation is associated with an increase in exchange activity. The phosphorylation state may regulate the binding to NHE1.
Sequence similarities	Contains 4 EF-hand domains.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Repeat
Ligand	Calcium Metal-binding
PTM	Lipoprotein Myristate Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	potassium ion transport Traceable author statement. Source: ProtInc small GTPase mediated signal transduction Traceable author statement. Source: ProtInc
Molecular function	potassium channel regulator activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed By similarity

Chain

2 – 195

194

Calcium-binding protein p22

PRO_0000073843

Regions

Domain

26 – 61

36

EF-hand 1

Domain

66 – 101

36

EF-hand 2

Domain

110 – 145

36

EF-hand 3

Domain

151 – 186

36

EF-hand 4

Calcium binding

123 – 134

12

1

Calcium binding

164 – 175

12

2

Amino acid modifications

Lipidation

2

1

N-myristoyl glycine By similarity

Secondary structure

1

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195

Helix Strand Turn

Details...

Helix

11 – 22

12

Helix

26 – 37

12

Turn

38 – 41

4

Helix

48 – 51

4

Helix

57 – 59

3

Helix

64 – 70

7

Helix

80 – 88

9

Beta strand

95 – 98

4

Beta strand

107 – 109

3

Helix

111 – 122

12

Beta strand

127 – 130

4

Helix

132 – 143

12

Turn

144 – 146

3

Helix

149 – 163

15

Beta strand

165 – 169

5

Beta strand

171 – 173

3

Helix

174 – 180

7

Helix

185 – 188

4

Sequences

	Sequence		Length	Mass (Da)
	Q99653 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 8E82EEF0CA5E832F	FASTA	195	22,456
	10 20 30 40 50 60 MGSRASTLLR DEELEEIKKE TGFSHSQITR LYSRFTSLDK GENGTLSRED FQRIPELAIN 70 80 90 100 110 120 PLGDRIINAF FPEGEDQVNF RGFMRTLAHF RPIEDNEKSK DVNGPEPLNS RSNKLHFAFR 130 140 150 160 170 180 LYDLDKDEKI SRDELLQVLR MMVGVNISDE QLGSIADRTI QEADQDGDSA ISFTEFVKVL 190 EKVDVEQKMS IRFLH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A calcineurin homologous protein inhibits GTPase-stimulated Na-H exchange." Lin X., Barber D.L. Proc. Natl. Acad. Sci. U.S.A. 93:12631-12636(1996) [PubMed: 8901634] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH SLC9A1. Tissue: B-cell.
[2]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Uterus.
[6]	"Inhibition of calcineurin phosphatase activity by a calcineurin B homologous protein." Lin X., Sikkink R.A., Rusnak F., Barber D.L. J. Biol. Chem. 274:36125-36131(1999) [PubMed: 10593895] [Abstract] Cited for: INHIBITION OF CALCINEURIN.
[7]	"Calcineurin homologous protein as an essential cofactor for Na+/H+ exchangers." Pang T., Su X., Wakabayashi S., Shigekawa M. J. Biol. Chem. 276:17367-17372(2001) [PubMed: 11350981] [Abstract] Cited for: FUNCTION.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]	"Solution structure of the cytoplasmic region of Na+/H+ exchanger 1 complexed with essential cofactor calcineurin B homologous protein 1." Mishima M., Wakabayashi S., Kojima C. J. Biol. Chem. 282:2741-2751(2007) [PubMed: 17050540] [Abstract] Cited for: STRUCTURE BY NMR OF 1-195 IN COMPLEX WITH CALCIUM IONS AND SLC9A1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U61538 mRNA. Translation: AAB37770.1.
CR536539 mRNA. Translation: CAG38776.1.
CR542085 mRNA. Translation: CAG46882.1.
AK312582 mRNA. Translation: BAG35476.1.
CH471125 Genomic DNA. Translation: EAW92474.1.
BC031293 mRNA. Translation: AAH31293.1.
BC051815 mRNA. No translation available.

IPI

IPI00218924.

RefSeq

NP_009167.1. NM_007236.4.

UniGene

Hs.406234.
Hs.706184.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2E30	NMR	-	A	1-195	[»]

ProteinModelPortal

Q99653.

SMR

Q99653. Positions 1-195.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q99653. 3 interactions.

MINT

MINT-1375478.

STRING

Q99653.

PTM databases

PhosphoSite

Q99653.

Polymorphism databases

DMDM

3023439.

2D gel databases

OGP

Q99653.

Proteomic databases

PeptideAtlas

Q99653.

PRIDE

Q99653.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000334660; ENSP00000335632; ENSG00000187446.

GeneID

11261.

KEGG

hsa:11261.

UCSC

uc001znl.1. human.

Organism-specific databases

CTD

11261.

GeneCards

GC15P041523.

H-InvDB

HIX0012148.

HPA

HPA006616.

MIM

606988. gene.

neXtProt

NX_Q99653.

Phylogenomic databases

eggNOG

prNOG07134.

GeneTree

ENSGT00600000084053.

HOGENOM

HBG746798.

HOVERGEN

HBG105307.

InParanoid

Q99653.

OMA

MYDLDDD.

OrthoDB

EOG4PG61X.

PhylomeDB

Q99653.

Enzyme and pathway databases

Pathway_Interaction_DB

tcrcalciumpathway. Calcium signaling in the CD4+ TCR pathway.
nfat_3pathway. Role of Calcineurin-dependent NFAT signaling in lymphocytes.

Gene expression databases

ArrayExpress

Q99653.

Bgee

Q99653.

Genevestigator

Q99653.

Family and domain databases

InterPro

IPR018248. EF-hand.
IPR011992. EF-hand-like_dom.
IPR018249. EF_HAND_2.
IPR002048. EF_hand_Ca-bd.
[Graphical view]

Gene3D

G3DSA:1.10.238.10. EF-Hand_type. 1 hit.

KO

K06268.

Pfam

PF00036. efhand. 1 hit.
[Graphical view]

SMART

SM00054. EFh. 2 hits.
[Graphical view]

PROSITE

PS00018. EF_HAND_1. False negative.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]

ProtoNet

Search...

Other

NextBio

42856.

SOURCE

Search...

Entry information

Entry name

CHP1_HUMAN

Accession

Primary (citable) accession number: Q99653
Secondary accession number(s): B2R6H9, Q6FHZ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 113 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.