Q99645 (EPYC_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Epiphycan

Alternative name(s):
Dermatan sulfate proteoglycan 3
Proteoglycan-Lb
Short name=PG-Lb
Small chondroitin/dermatan sulfate proteoglycan

Gene names

Name:	EPYC
Synonyms:	DSPG3, PGLB, SLRR3B

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	322 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May have a role in bone formation and also in establishing the ordered structure of cartilage through matrix organization.
Subcellular location	Secreted › extracellular space › extracellular matrix By similarity.
Tissue specificity	Cartilage, ligament, and placenta. Ref.1
Post-translational modification	The O-linked polysaccharides on Thr-60 and Ser-96 are probably the mucin type linked to GalNAc. There is one glycosaminoglycan chain, known to be dermatan sulfate, and it is probably the O-glycosylation at Ser-64 By similarity.
Sequence similarities	Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class III subfamily. Contains 6 LRR (leucine-rich) repeats. Contains 1 LRRNT domain.

Ontologies

Keywords
Cellular component	Extracellular matrix Secreted
Coding sequence diversity	Polymorphism
Domain	Leucine-rich repeat Repeat Signal
PTM	Disulfide bond Glycoprotein Proteoglycan
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	female pregnancy Traceable author statement Ref.1. Source: ProtInc
Cellular_component	proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glycosaminoglycan binding Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 322	303	Epiphycan		PRO_0000032768
Regions
	Domain	106 – 143	38	LRRNT
	Repeat	144 – 165	22	LRR 1
	Repeat	168 – 189	22	LRR 2
	Repeat	192 – 213	22	LRR 3
	Repeat	238 – 258	21	LRR 4
	Repeat	259 – 280	22	LRR 5
	Repeat	290 – 310	21	LRR 6
	Compositional bias	81 – 87	7	Poly-Glu
Amino acid modifications
	Glycosylation	60	1	O-linked (GalNAc...) By similarity
	Glycosylation	64	1	O-linked (Xyl...) (dermatan sulfate) By similarity
	Glycosylation	96	1	O-linked (GalNAc...) By similarity
	Glycosylation	283	1	N-linked (GlcNAc...) Potential
	Glycosylation	302	1	N-linked (GlcNAc...) Potential
	Disulfide bond	118 ↔ 130		By similarity
	Disulfide bond	279 ↔ 312		By similarity
Natural variations
	Natural variant	150	1	S → C. Corresponds to variant rs17784152 [ dbSNP | Ensembl ].		VAR_031595
Experimental info
	Sequence conflict	14	1	F → L in AAC50945. Ref.1
	Sequence conflict	52	1	D → G in AAH30958. Ref.4
	Sequence conflict	120	1	L → W in AAC50945. Ref.1
	Sequence conflict	213	1	L → S in AAC50945. Ref.1
	Sequence conflict	281	1	V → G in AAC50945. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q99645 [UniParc]. Last modified April 3, 2007. Version 3. Checksum: E11061A38AA7240E	FASTA	322	36,637
	10 20 30 40 50 60 MKTLAGLVLG LVIFDAAVTA PTLESINYDS ETYDATLEDL DNLYNYENIP VDKVEIEIAT 70 80 90 100 110 120 VMPSGNRELL TPPPQPEKAQ EEEEEEESTP RLIDGSSPQE PEFTGVLGPH TNEDFPTCLL 130 140 150 160 170 180 CTCISTTVYC DDHELDAIPP LPKNTAYFYS RFNRIKKINK NDFASLSDLK RIDLTSNLIS 190 200 210 220 230 240 EIDEDAFRKL PQLRELVLRD NKIRQLPELP TTLTFIDISN NRLGRKGIKQ EAFKDMYDLH 250 260 270 280 290 300 HLYLTDNNLD HIPLPLPENL RALHLQNNNI LEMHEDTFCN VKNLTYIRKA LEDIRLDGNP 310 320 INLSKTPQAY MCLPRLPVGS LV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of human DSPG3, a small dermatan sulfate proteoglycan." Deere M., Johnson H.J., Garza S., Harrison W.R., Yoon S.-J., Elder F.F.B., Kucherlapati R., Hoeoek M., Hecht J.T. Genomics 38:399-404(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Chondrocyte.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U59111 mRNA. Translation: AAC50945.1. AK290642 mRNA. Translation: BAF83331.1. CH471054 Genomic DNA. Translation: EAW97444.1. BC030958 mRNA. Translation: AAH30958.1.
CCDS	CCDS31870.1.
RefSeq	NP_004941.2. NM_004950.4.
UniGene	Hs.435680.
3D structure databases
ProteinModelPortal	Q99645.
SMR	Q99645. Positions 121-316.
ModBase	Search...
MobiDB	Search...
Chemistry
BindingDB	Q99645.
PTM databases
PhosphoSite	Q99645.
Polymorphism databases
DMDM	143811386.
Proteomic databases
PaxDb	Q99645.
PRIDE	Q99645.
Protocols and materials databases
DNASU	1833.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000261172; ENSP00000261172; ENSG00000083782.
GeneID	1833.
KEGG	hsa:1833.
UCSC	uc001tbk.3. human.
Organism-specific databases
CTD	1833.
GeneCards	GC12M091357.
HGNC	HGNC:3053. EPYC.
HPA	HPA038212. HPA045455.
MIM	601657. gene.
neXtProt	NX_Q99645.
PharmGKB	PA162385144.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG314832.
HOGENOM	HOG000273909.
HOVERGEN	HBG006850.
InParanoid	Q99645.
KO	K08127.
OMA	CDDHELD.
OrthoDB	EOG757CXS.
PhylomeDB	Q99645.
TreeFam	TF351924.
Gene expression databases
ArrayExpress	Q99645.
Bgee	Q99645.
CleanEx	HS_EPYC.
Genevestigator	Q99645.
Family and domain databases
InterPro	IPR027217. Epiphycan. IPR001611. Leu-rich_rpt. IPR000372. LRR-contain_N. [Graphical view]
PANTHER	PTHR24371:SF4. PTHR24371:SF4. 1 hit.
Pfam	PF13855. LRR_8. 1 hit. PF01462. LRRNT. 1 hit. [Graphical view]
SMART	SM00013. LRRNT. 1 hit. [Graphical view]
PROSITE	PS51450. LRR. 4 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	1833.
NextBio	7487.
PRO	Q99645.
SOURCE	Search...

Entry information

Entry name

EPYC_HUMAN

Accession

Primary (citable) accession number: Q99645
Secondary accession number(s): A8K3M7, Q8NEJ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	April 3, 2007
Last modified:	July 9, 2014
This is version 129 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.