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Q99640

- PMYT1_HUMAN

UniProt

Q99640 - PMYT1_HUMAN

Protein

Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase

Gene

PKMYT1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 135 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
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    Functioni

    Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development.2 Publications

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Enzyme regulationi

    Negatively regulated by hyperphosphorylation during mitosis. The hyperphosphorylated form does not associate with CCNB1-CDC2 complexes. The PLK1 protein kinase may be required for mitotic phosphorylation.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei139 – 1391ATPPROSITE-ProRule annotation
    Active sitei233 – 2331Proton acceptorPROSITE-ProRule annotation
    Metal bindingi238 – 2381Magnesium; via carbonyl oxygenBy similarity
    Metal bindingi251 – 2511Magnesium; via carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi116 – 1249ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. kinase activity Source: HGNC
    3. metal ion binding Source: UniProtKB-KW
    4. protein binding Source: IntAct
    5. protein kinase activity Source: ProtInc
    6. protein serine/threonine kinase activity Source: UniProtKB-KW

    GO - Biological processi

    1. G1/S transition of mitotic cell cycle Source: Reactome
    2. G2/M transition of mitotic cell cycle Source: Reactome
    3. mitotic cell cycle Source: Reactome
    4. mitotic nuclear division Source: ProtInc
    5. negative regulation of phosphatase activity Source: UniProtKB
    6. regulation of cell cycle Source: Reactome
    7. regulation of cyclin-dependent protein serine/threonine kinase activity Source: ProtInc
    8. regulation of mitosis Source: ProtInc

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Cell cycle

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_1006. Polo-like kinase mediated events.
    REACT_1846. G2/M DNA replication checkpoint.
    REACT_1857. Cyclin A/B1 associated events during G2/M transition.
    SignaLinkiQ99640.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase (EC:2.7.11.1)
    Alternative name(s):
    Myt1 kinase
    Gene namesi
    Name:PKMYT1
    Synonyms:MYT1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 16

    Organism-specific databases

    HGNCiHGNC:29650. PKMYT1.

    Subcellular locationi

    Endoplasmic reticulum membrane 1 Publication; Peripheral membrane protein 1 Publication. Golgi apparatus membrane 1 Publication; Peripheral membrane protein 1 Publication

    GO - Cellular componenti

    1. cytosol Source: Reactome
    2. endoplasmic reticulum Source: ProtInc
    3. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    4. Golgi apparatus Source: ProtInc
    5. Golgi membrane Source: UniProtKB-SubCell
    6. membrane Source: UniProtKB
    7. nucleoplasm Source: Reactome

    Keywords - Cellular componenti

    Endoplasmic reticulum, Golgi apparatus, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi238 – 2381N → A: Loss of kinase activity. 1 Publication
    Mutagenesisi251 – 2511D → A: Loss of kinase activity. 1 Publication
    Mutagenesisi486 – 4883RNL → AAA: Loss of CDC2-CCNB1 interaction.

    Organism-specific databases

    PharmGKBiPA385.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 499499Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinasePRO_0000086573Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine1 Publication
    Modified residuei17 – 171Phosphothreonine3 Publications
    Modified residuei94 – 941Phosphoserine2 Publications
    Modified residuei120 – 1201Phosphoserine2 Publications
    Modified residuei160 – 1601Phosphoserine2 Publications
    Modified residuei426 – 4261Phosphoserine; by PLK12 Publications
    Modified residuei469 – 4691Phosphoserine3 Publications
    Modified residuei473 – 4731Phosphoserine2 Publications
    Modified residuei495 – 4951Phosphothreonine; by PLK13 Publications

    Post-translational modificationi

    Autophosphorylated. Phosphorylated by CDC2-CCNB1 complexes on undefined serine and threonine residues. The phosphorylation by CDC2-CCNB1 complexes may inhibit the catalytic activity.5 Publications

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ99640.
    PaxDbiQ99640.
    PRIDEiQ99640.

    PTM databases

    PhosphoSiteiQ99640.

    Expressioni

    Gene expression databases

    ArrayExpressiQ99640.
    BgeeiQ99640.
    CleanExiHS_MYT1.
    HS_PKMYT1.
    GenevestigatoriQ99640.

    Organism-specific databases

    HPAiCAB046000.

    Interactioni

    Subunit structurei

    Interacts with CDC2-CCNB1 complex. Can also interact with PIN1 when phosphorylated by CDC2-CCNB1.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CCNB1P146354EBI-495308,EBI-495332
    CDK1P064933EBI-495308,EBI-444308

    Protein-protein interaction databases

    BioGridi114544. 9 interactions.
    IntActiQ99640. 6 interactions.
    MINTiMINT-3059438.
    STRINGi9606.ENSP00000262300.

    Structurei

    Secondary structure

    1
    499
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi101 – 1033
    Helixi105 – 1095
    Beta strandi110 – 11910
    Beta strandi122 – 1298
    Turni130 – 1323
    Beta strandi135 – 14410
    Helixi148 – 16417
    Beta strandi173 – 1797
    Beta strandi182 – 1887
    Helixi194 – 2018
    Helixi207 – 22620
    Helixi236 – 2383
    Beta strandi239 – 2413
    Helixi243 – 2453
    Beta strandi247 – 2493
    Helixi271 – 2733
    Helixi276 – 2805
    Helixi286 – 30116
    Helixi309 – 3157
    Turni316 – 3183
    Helixi322 – 3254
    Helixi330 – 33910
    Turni344 – 3463
    Helixi350 – 3545
    Helixi357 – 3593

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3P1AX-ray1.70A75-362[»]
    ProteinModelPortaliQ99640.
    SMRiQ99640. Positions 76-362.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ99640.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini110 – 359250Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni398 – 499102Interaction with PIN1Add
    BLAST
    Regioni437 – 49963Interaction with CDC2-CCNB1Add
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi382 – 39817Membrane-association motifAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi5 – 7268Pro-richAdd
    BLAST

    Domaini

    The membrane-association motif is essential for the localization to membrane of Golgi stack. According to some authors, it is a transmembrane domain; the existence of a transmembrane region is however unproven.

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000293277.
    HOVERGENiHBG053623.
    InParanoidiQ99640.
    KOiK06633.
    PhylomeDBiQ99640.
    TreeFamiTF101087.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR008271. Ser/Thr_kinase_AS.
    IPR016235. Tyr/Thr_kinase_Cdc2_inhib.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000567. TYPK_Myt1. 1 hit.
    SUPFAMiSSF56112. SSF56112. 2 hits.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequences (4)i

    Sequence statusi: Complete.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q99640-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MLERPPALAM PMPTEGTPPP LSGTPIPVPA YFRHAEPGFS LKRPRGLSRS    50
    LPPPPPAKGS IPISRLFPPR TPGWHQLQPR RVSFRGEASE TLQSPGYDPS 100
    RPESFFQQSF QRLSRLGHGS YGEVFKVRSK EDGRLYAVKR SMSPFRGPKD 150
    RARKLAEVGS HEKVGQHPCC VRLEQAWEEG GILYLQTELC GPSLQQHCEA 200
    WGASLPEAQV WGYLRDTLLA LAHLHSQGLV HLDVKPANIF LGPRGRCKLG 250
    DFGLLVELGT AGAGEVQEGD PRYMAPELLQ GSYGTAADVF SLGLTILEVA 300
    CNMELPHGGE GWQQLRQGYL PPEFTAGLSS ELRSVLVMML EPDPKLRATA 350
    EALLALPVLR QPRAWGVLWC MAAEALSRGW ALWQALLALL CWLWHGLAHP 400
    ASWLQPLGPP ATPPGSPPCS LLLDSSLSSN WDDDSLGPSL SPEAVLARTV 450
    GSTSTPRSRC TPRDALDLSD INSEPPRGSF PSFEPRNLLS LFEDTLDPT 499
    Length:499
    Mass (Da):54,521
    Last modified:May 1, 1997 - v1
    Checksum:i4DF28A5965265567
    GO
    Isoform 2 (identifier: Q99640-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         439-499: SLSPEAVLAR...SLFEDTLDPT → GHPPCLACPP...AGAHPGMPWT

    Note: No experimental confirmation available.

    Show »
    Length:480
    Mass (Da):52,146
    Checksum:iE3A040B3858812A6
    GO
    Isoform 3 (identifier: Q99640-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-9: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:490
    Mass (Da):53,542
    Checksum:i0D60B306B464E780
    GO
    Isoform 4 (identifier: Q99640-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         5-73: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:430
    Mass (Da):47,284
    Checksum:iCD5FC8DADE1E8E7E
    GO

    Sequence cautioni

    The sequence CAD28540.1 differs from that shown. Reason: Chimeric cDNA.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti131 – 1311E → A in BAG53500. 1 PublicationCurated
    Sequence conflicti415 – 4151G → D in AAB71843. (PubMed:9268380)Curated
    Sequence conflicti427 – 4271L → F in AAB71843. (PubMed:9268380)Curated
    Sequence conflicti491 – 4911L → M in AAB71843. (PubMed:9268380)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti103 – 1031E → Q.1 Publication
    Corresponds to variant rs55834293 [ dbSNP | Ensembl ].
    VAR_041034
    Natural varianti140 – 1401R → C.2 Publications
    Corresponds to variant rs4149796 [ dbSNP | Ensembl ].
    VAR_019928
    Natural varianti246 – 2461R → H.1 Publication
    Corresponds to variant rs35192104 [ dbSNP | Ensembl ].
    VAR_041035
    Natural varianti351 – 3511E → K.1 Publication
    Corresponds to variant rs56382954 [ dbSNP | Ensembl ].
    VAR_041036
    Natural varianti417 – 4171P → R.1 Publication
    Corresponds to variant rs4149800 [ dbSNP | Ensembl ].
    VAR_019929
    Natural varianti445 – 4451V → A.1 Publication
    Corresponds to variant rs10546 [ dbSNP | Ensembl ].
    VAR_019930

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 99Missing in isoform 3. 1 PublicationVSP_046846
    Alternative sequencei5 – 7369Missing in isoform 4. 1 PublicationVSP_046847Add
    BLAST
    Alternative sequencei439 – 49961SLSPE…TLDPT → GHPPCLACPPAGLHSPLRLS WPGLWGAPPPPGAGAHPGMP WT in isoform 2. 1 PublicationVSP_045699Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF014118 mRNA. Translation: AAB71843.1.
    U56816 mRNA. Translation: AAC50949.1.
    AK097642 mRNA. Translation: BAG53500.1.
    AK098452 mRNA. No translation available.
    AK301926 mRNA. Translation: BAG63346.1.
    AF549406 Genomic DNA. Translation: AAN40703.1.
    AC004233 Genomic DNA. Translation: AAC04478.1.
    AC004235 Genomic DNA. Translation: AAC04477.1.
    CH471112 Genomic DNA. Translation: EAW85439.1.
    CH471112 Genomic DNA. Translation: EAW85440.1.
    AL713779 mRNA. Translation: CAD28540.1. Sequence problems.
    CCDSiCCDS10486.1. [Q99640-1]
    CCDS45391.1. [Q99640-2]
    CCDS58414.1. [Q99640-3]
    CCDS58415.1. [Q99640-4]
    RefSeqiNP_001245379.1. NM_001258450.1. [Q99640-4]
    NP_001245380.1. NM_001258451.1. [Q99640-3]
    NP_004194.3. NM_004203.4. [Q99640-1]
    NP_872629.1. NM_182687.2. [Q99640-2]
    XP_006721039.1. XM_006720976.1. [Q99640-1]
    UniGeneiHs.732385.

    Genome annotation databases

    EnsembliENST00000262300; ENSP00000262300; ENSG00000127564. [Q99640-1]
    ENST00000440027; ENSP00000397739; ENSG00000127564. [Q99640-2]
    ENST00000573944; ENSP00000459123; ENSG00000127564. [Q99640-3]
    ENST00000574385; ENSP00000458943; ENSG00000127564. [Q99640-3]
    ENST00000574730; ENSP00000460868; ENSG00000127564. [Q99640-4]
    GeneIDi9088.
    KEGGihsa:9088.
    UCSCiuc002csn.3. human. [Q99640-1]

    Polymorphism databases

    DMDMi55976573.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Web resourcesi

    NIEHS-SNPs

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF014118 mRNA. Translation: AAB71843.1 .
    U56816 mRNA. Translation: AAC50949.1 .
    AK097642 mRNA. Translation: BAG53500.1 .
    AK098452 mRNA. No translation available.
    AK301926 mRNA. Translation: BAG63346.1 .
    AF549406 Genomic DNA. Translation: AAN40703.1 .
    AC004233 Genomic DNA. Translation: AAC04478.1 .
    AC004235 Genomic DNA. Translation: AAC04477.1 .
    CH471112 Genomic DNA. Translation: EAW85439.1 .
    CH471112 Genomic DNA. Translation: EAW85440.1 .
    AL713779 mRNA. Translation: CAD28540.1 . Sequence problems.
    CCDSi CCDS10486.1. [Q99640-1 ]
    CCDS45391.1. [Q99640-2 ]
    CCDS58414.1. [Q99640-3 ]
    CCDS58415.1. [Q99640-4 ]
    RefSeqi NP_001245379.1. NM_001258450.1. [Q99640-4 ]
    NP_001245380.1. NM_001258451.1. [Q99640-3 ]
    NP_004194.3. NM_004203.4. [Q99640-1 ]
    NP_872629.1. NM_182687.2. [Q99640-2 ]
    XP_006721039.1. XM_006720976.1. [Q99640-1 ]
    UniGenei Hs.732385.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3P1A X-ray 1.70 A 75-362 [» ]
    ProteinModelPortali Q99640.
    SMRi Q99640. Positions 76-362.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114544. 9 interactions.
    IntActi Q99640. 6 interactions.
    MINTi MINT-3059438.
    STRINGi 9606.ENSP00000262300.

    Chemistry

    BindingDBi Q99640.
    ChEMBLi CHEMBL3984.
    GuidetoPHARMACOLOGYi 2167.

    PTM databases

    PhosphoSitei Q99640.

    Polymorphism databases

    DMDMi 55976573.

    Proteomic databases

    MaxQBi Q99640.
    PaxDbi Q99640.
    PRIDEi Q99640.

    Protocols and materials databases

    DNASUi 9088.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000262300 ; ENSP00000262300 ; ENSG00000127564 . [Q99640-1 ]
    ENST00000440027 ; ENSP00000397739 ; ENSG00000127564 . [Q99640-2 ]
    ENST00000573944 ; ENSP00000459123 ; ENSG00000127564 . [Q99640-3 ]
    ENST00000574385 ; ENSP00000458943 ; ENSG00000127564 . [Q99640-3 ]
    ENST00000574730 ; ENSP00000460868 ; ENSG00000127564 . [Q99640-4 ]
    GeneIDi 9088.
    KEGGi hsa:9088.
    UCSCi uc002csn.3. human. [Q99640-1 ]

    Organism-specific databases

    CTDi 9088.
    GeneCardsi GC16M002995.
    HGNCi HGNC:29650. PKMYT1.
    HPAi CAB046000.
    MIMi 602474. gene.
    neXtProti NX_Q99640.
    PharmGKBi PA385.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000293277.
    HOVERGENi HBG053623.
    InParanoidi Q99640.
    KOi K06633.
    PhylomeDBi Q99640.
    TreeFami TF101087.

    Enzyme and pathway databases

    Reactomei REACT_1006. Polo-like kinase mediated events.
    REACT_1846. G2/M DNA replication checkpoint.
    REACT_1857. Cyclin A/B1 associated events during G2/M transition.
    SignaLinki Q99640.

    Miscellaneous databases

    ChiTaRSi PKMYT1. human.
    EvolutionaryTracei Q99640.
    GeneWikii PKMYT1.
    GenomeRNAii 9088.
    NextBioi 34055.
    PROi Q99640.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q99640.
    Bgeei Q99640.
    CleanExi HS_MYT1.
    HS_PKMYT1.
    Genevestigatori Q99640.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR008271. Ser/Thr_kinase_AS.
    IPR016235. Tyr/Thr_kinase_Cdc2_inhib.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000567. TYPK_Myt1. 1 hit.
    SUPFAMi SSF56112. SSF56112. 2 hits.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Human Myt1 is a cell cycle-regulated kinase that inhibits Cdc2 but not Cdk2 activity."
      Booher R.N., Holman P.S., Fattaey A.
      J. Biol. Chem. 272:22300-22306(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ENZYME REGULATION.
    2. "The human Myt1 kinase preferentially phosphorylates Cdc2 on threonine 14 and localizes to the endoplasmic reticulum and Golgi complex."
      Liu F., Stanton J.J., Wu Z., Piwnica-Worms H.
      Mol. Cell. Biol. 17:571-583(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION.
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2; 3 AND 4).
      Tissue: Testis and Thyroid.
    4. NIEHS SNPs program
      Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS CYS-140; ARG-417 AND ALA-445.
    5. "The sequence and analysis of duplication-rich human chromosome 16."
      Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J.
      , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A.
      Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 403-499 (ISOFORM 1).
      Tissue: Brain.
    8. "The essential mitotic peptidyl-prolyl isomerase Pin1 binds and regulates mitosis-specific phosphoproteins."
      Shen M., Stukenberg P.T., Kirschner M.W., Lu K.P.
      Genes Dev. 12:706-720(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PIN1.
    9. "The C-terminal domain of the Cdc2 inhibitory kinase Myt1 interacts with Cdc2 complexes and is required for inhibition of G(2)/M progression."
      Wells N.J., Watanabe N., Tokusumi T., Jiang W., Verdecia M.A., Hunter T.
      J. Cell Sci. 112:3361-3371(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION, COMPONENT OF CDC2-CCNB1 COMPLEX, INTERACTION WITH PIN1, MUTAGENESIS OF ASP-251.
    10. "Overproduction of human Myt1 kinase induces a G2 cell cycle delay by interfering with the intracellular trafficking of Cdc2-cyclin B1 complexes."
      Liu F., Rothblum-Oviatt C., Ryan C.E., Piwnica-Worms H.
      Mol. Cell. Biol. 19:5113-5123(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF ASN-238 AND 486-ARG--LEU-488.
    11. "Identification of a consensus motif for Plk (Polo-like kinase) phosphorylation reveals Myt1 as a Plk1 substrate."
      Nakajima H., Toyoshima-Morimoto F., Taniguchi E., Nishida E.
      J. Biol. Chem. 278:25277-25280(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT SER-426 AND THR-495.
    12. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
      Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
      Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-17; SER-94; SER-469 AND THR-495, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    13. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-120; SER-469 AND SER-473, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    14. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-17 AND SER-160, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    15. "Patterns of somatic mutation in human cancer genomes."
      Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G.
      , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
      Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANTS [LARGE SCALE ANALYSIS] GLN-103; CYS-140; HIS-246 AND LYS-351.

    Entry informationi

    Entry nameiPMYT1_HUMAN
    AccessioniPrimary (citable) accession number: Q99640
    Secondary accession number(s): B3KUN8
    , B4DXD4, D3DUA4, F8W164, I3L1V2, O14731, Q7LE24, Q8TCM9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 23, 2004
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 135 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 16
      Human chromosome 16: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    7. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3