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Q99633 (PRP18_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pre-mRNA-splicing factor 18
Alternative name(s):
PRP18 homolog
Short name=hPRP18
Gene names
Name:PRPF18
Synonyms:HPRP18
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length342 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Participates in the second step of pre-mRNA splicing. Ref.1

Subunit structure

Heterodimer with PPIH. Interacts with PRPF4 and with the spliceosome. Part of a complex containing U4/U6 snRNPs. Ref.1 Ref.5

Subcellular location

Nucleus speckle By similarity. Note: Colocalizes with spliceosomal snRNPs By similarity.

Sequence similarities

Belongs to the PRP18 family.

Ontologies

Keywords
   Biological processmRNA processing
mRNA splicing
   Cellular componentNucleus
Spliceosome
   Coding sequence diversityAlternative splicing
   PTMAcetylation
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processRNA splicing

Traceable author statement Ref.1. Source: ProtInc

mRNA processing

Traceable author statement Ref.1. Source: ProtInc

   Cellular_componentnuclear speck

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Traceable author statement Ref.1. Source: ProtInc

spliceosomal complex

Traceable author statement Ref.1. Source: ProtInc

   Molecular_functionpotassium channel inhibitor activity

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q99633-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q99633-2)

The sequence of this isoform differs from the canonical sequence as follows:
     22-259: Missing.
     286-342: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 342342Pre-mRNA-splicing factor 18
PRO_0000058582

Amino acid modifications

Modified residue11N-acetylmethionine Ref.6

Natural variations

Alternative sequence22 – 259238Missing in isoform 2.
VSP_008327
Alternative sequence286 – 34257Missing in isoform 2.
VSP_008328

Secondary structure

......... 342
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 269D79E1E733CF6D

FASTA34239,860
        10         20         30         40         50         60 
MDILKSEILR KRQLVEDRNL LVENKKYFKR SELAKKEEEA YFERCGYKIQ PKEEDQKPLT 

        70         80         90        100        110        120 
SSNPVLELEL AEEKLPMTLS RQEVIRRLRE RGEPIRLFGE TDYDAFQRLR KIEILTPEVN 

       130        140        150        160        170        180 
KGLRNDLKAA LDKIDQQYLN EIVGGQEPGE EDTQNDLKVH EENTTIEELE ALGESLGKGD 

       190        200        210        220        230        240 
DHKDMDIITK FLKFLLGVWA KELNAREDYV KRSVQGKLNS ATQKQTESYL RPLFRKLRKR 

       250        260        270        280        290        300 
NLPADIKESI TDIIKFMLQR EYVKANDAYL QMAIGNAPWP IGVTMVGIHA RTGREKIFSK 

       310        320        330        340 
HVAHVLNDET QRKYIQGLKR LMTICQKHFP TDPSKCVEYN AL 

« Hide

Isoform 2 [UniParc].

Checksum: 3A4FB4FF31181C8A
Show »

FASTA475,474

References

« Hide 'large scale' references
[1]"A human protein required for the second step of pre-mRNA splicing is functionally related to a yeast splicing factor."
Horowitz D.S., Krainer A.R.
Genes Dev. 11:139-151(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH PRPF4 AND THE SPLICEOSOME.
Tissue: Cervix carcinoma.
[2]"The DNA sequence and comparative analysis of human chromosome 10."
Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. expand/collapse author list , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Brain and Placenta.
[5]"A cyclophilin functions in pre-mRNA splicing."
Horowitz D.S., Lee E.J., Mabon S.A., Misteli T.
EMBO J. 21:470-480(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PPIH.
[6]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"Solution structure of splicing factor motif in pre-mRNA splicing factor 18 (HPRP18)."
RIKEN structural genomics initiative (RSGI)
Submitted (OCT-2006) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 60-122.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U51990 mRNA. Translation: AAB41490.1.
AL157392 Genomic DNA. Translation: CAI13123.1.
CH471072 Genomic DNA. Translation: EAW86281.1.
BC000794 mRNA. Translation: AAH00794.1.
BC002572 mRNA. No translation available.
CCDSCCDS7100.1. [Q99633-1]
RefSeqNP_003666.1. NM_003675.3. [Q99633-1]
UniGeneHs.729158.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2DK4NMR-A60-122[»]
ProteinModelPortalQ99633.
SMRQ99633. Positions 63-122.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid114129. 3 interactions.
IntActQ99633. 1 interaction.
STRING9606.ENSP00000367835.

PTM databases

PhosphoSiteQ99633.

Polymorphism databases

DMDM37082244.

Proteomic databases

MaxQBQ99633.
PaxDbQ99633.
PRIDEQ99633.

Protocols and materials databases

DNASU8559.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000378572; ENSP00000367835; ENSG00000165630. [Q99633-1]
GeneID8559.
KEGGhsa:8559.
UCSCuc001imp.3. human. [Q99633-1]

Organism-specific databases

CTD8559.
GeneCardsGC10P013628.
HGNCHGNC:17351. PRPF18.
HPAHPA037599.
MIM604993. gene.
neXtProtNX_Q99633.
PharmGKBPA38449.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG313018.
HOGENOMHOG000242389.
HOVERGENHBG053696.
InParanoidQ99633.
KOK12817.
OMAYFKRGEL.
OrthoDBEOG7034HM.
PhylomeDBQ99633.
TreeFamTF315049.

Gene expression databases

ArrayExpressQ99633.
BgeeQ99633.
CleanExHS_PRPF18.
GenevestigatorQ99633.

Family and domain databases

Gene3D1.20.940.10. 1 hit.
InterProIPR004098. Prp18.
IPR014906. PRP4-like.
IPR003648. SFM.
[Graphical view]
PfamPF02840. Prp18. 1 hit.
PF08799. PRP4. 1 hit.
[Graphical view]
SMARTSM00500. SFM. 1 hit.
[Graphical view]
SUPFAMSSF47938. SSF47938. 1 hit.
ProtoNetSearch...

Other

ChiTaRSPRPF18. human.
EvolutionaryTraceQ99633.
GenomeRNAi8559.
NextBio32085.
PROQ99633.
SOURCESearch...

Entry information

Entry namePRP18_HUMAN
AccessionPrimary (citable) accession number: Q99633
Secondary accession number(s): Q5T9P9, Q9BUI9
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: May 1, 1997
Last modified: July 9, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 10

Human chromosome 10: entries, gene names and cross-references to MIM