Q99633 (PRP18_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pre-mRNA-splicing factor 18

Alternative name(s):
PRP18 homolog
Short name=hPRP18

Gene names

Name:	PRPF18
Synonyms:	HPRP18

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	342 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Participates in the second step of pre-mRNA splicing. Ref.1
Subunit structure	Heterodimer with PPIH. Interacts with PRPF4 and with the spliceosome. Part of a complex containing U4/U6 snRNPs. Ref.1 Ref.5
Subcellular location	Nucleus speckle By similarity. Note: Colocalizes with spliceosomal snRNPs By similarity.
Sequence similarities	Belongs to the PRP18 family.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus Spliceosome
Coding sequence diversity	Alternative splicing
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA splicing Traceable author statement Ref.1. Source: ProtInc mRNA processing Traceable author statement Ref.1. Source: ProtInc
Cellular_component	nuclear speck Inferred from electronic annotation. Source: UniProtKB-SubCell spliceosomal complex Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q99633-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q99633-2)

The sequence of this isoform differs from the canonical sequence as follows:
     22-259: Missing.
     286-342: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 342

342

Pre-mRNA-splicing factor 18

PRO_0000058582

Amino acid modifications

Modified residue

41

1

Phosphotyrosine By similarity

Natural variations

Alternative sequence

22 – 259

238

Missing in isoform 2.

VSP_008327

Alternative sequence

286 – 342

57

Missing in isoform 2.

VSP_008328

Secondary structure

1

.

.

.

.

.

.

.

.

.

342

Helix Strand Turn

Details...

Beta strand

69 – 71

3

Helix

81 – 91

11

Helix

102 – 115

14

Beta strand

117 – 119

3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 269D79E1E733CF6D	FASTA	342	39,860
	10 20 30 40 50 60 MDILKSEILR KRQLVEDRNL LVENKKYFKR SELAKKEEEA YFERCGYKIQ PKEEDQKPLT 70 80 90 100 110 120 SSNPVLELEL AEEKLPMTLS RQEVIRRLRE RGEPIRLFGE TDYDAFQRLR KIEILTPEVN 130 140 150 160 170 180 KGLRNDLKAA LDKIDQQYLN EIVGGQEPGE EDTQNDLKVH EENTTIEELE ALGESLGKGD 190 200 210 220 230 240 DHKDMDIITK FLKFLLGVWA KELNAREDYV KRSVQGKLNS ATQKQTESYL RPLFRKLRKR 250 260 270 280 290 300 NLPADIKESI TDIIKFMLQR EYVKANDAYL QMAIGNAPWP IGVTMVGIHA RTGREKIFSK 310 320 330 340 HVAHVLNDET QRKYIQGLKR LMTICQKHFP TDPSKCVEYN AL
	Isoform 2 [UniParc]. Checksum: 3A4FB4FF31181C8A	FASTA	47	5,474
	10 20 30 40 MDILKSEILR KRQLVEDRNL LREYVKANDA YLQMAIGNAP WPIGVTM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A human protein required for the second step of pre-mRNA splicing is functionally related to a yeast splicing factor." Horowitz D.S., Krainer A.R. Genes Dev. 11:139-151(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH PRPF4 AND THE SPLICEOSOME. Tissue: Cervix carcinoma.
[2]	"The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J. Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain and Placenta.
[5]	"A cyclophilin functions in pre-mRNA splicing." Horowitz D.S., Lee E.J., Mabon S.A., Misteli T. EMBO J. 21:470-480(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PPIH.
[6]	"Solution structure of splicing factor motif in pre-mRNA splicing factor 18 (HPRP18)." RIKEN structural genomics initiative (RSGI) Submitted (OCT-2006) to the PDB data bank Cited for: STRUCTURE BY NMR OF 60-122.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U51990 mRNA. Translation: AAB41490.1.
AL157392 Genomic DNA. Translation: CAI13123.1.
CH471072 Genomic DNA. Translation: EAW86281.1.
BC000794 mRNA. Translation: AAH00794.1.
BC002572 mRNA. No translation available.

IPI

IPI00021146.
IPI00024021.

RefSeq

NP_003666.1. NM_003675.3.

UniGene

Hs.729158.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2DK4	NMR	-	A	60-122	[»]

ProteinModelPortal

Q99633.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q99633. 1 interaction.

STRING

9606.ENSP00000367835.

PTM databases

PhosphoSite

Q99633.

Polymorphism databases

DMDM

37082244.

Proteomic databases

PaxDb

Q99633.

PRIDE

Q99633.

Protocols and materials databases

DNASU

8559.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000378572; ENSP00000367835; ENSG00000165630.

GeneID

8559.

KEGG

hsa:8559.

UCSC

uc001imp.3. human.

Organism-specific databases

CTD

8559.

GeneCards

GC10P013628.

HGNC

HGNC:17351. PRPF18.

HPA

HPA037599.

MIM

604993. gene.

neXtProt

NX_Q99633.

PharmGKB

PA38449.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG313018.

HOGENOM

HOG000242389.

HOVERGEN

HBG053696.

InParanoid

Q99633.

KO

K12817.

OMA

RGFRNDF.

OrthoDB

EOG4J6RR4.

PhylomeDB

Q99633.

Gene expression databases

ArrayExpress

Q99633.

Bgee

Q99633.

CleanEx

HS_PRPF18.

Genevestigator

Q99633.

GermOnline

ENSG00000165630. Homo sapiens.

Family and domain databases

Gene3D

1.20.940.10. 1 hit.

InterPro

IPR004098. Prp18.
IPR014906. PRP4.
IPR003648. SFM.
[Graphical view]

Pfam

PF02840. Prp18. 1 hit.
PF08799. PRP4. 1 hit.
[Graphical view]

SMART

SM00500. SFM. 1 hit.
[Graphical view]

SUPFAM

SSF47938. Prp18. 1 hit.

ProtoNet

Search...

Other

ChiTaRS

PRPF18. human.

EvolutionaryTrace

Q99633.

GenomeRNAi

8559.

NextBio

32085.

SOURCE

Search...

Entry information

Entry name

PRP18_HUMAN

Accession

Primary (citable) accession number: Q99633
Secondary accession number(s): Q5T9P9, Q9BUI9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2003
Last sequence update:	May 1, 1997
Last modified:	May 1, 2013
This is version 109 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.