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Protein

Pre-mRNA-splicing factor 18

Gene

PRPF18

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Participates in the second step of pre-mRNA splicing.1 Publication

GO - Molecular functioni

  1. potassium channel inhibitor activity Source: Ensembl

GO - Biological processi

  1. mRNA processing Source: ProtInc
  2. RNA splicing Source: ProtInc
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor 18
Alternative name(s):
PRP18 homolog
Short name:
hPRP18
Gene namesi
Name:PRPF18
Synonyms:HPRP18
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 10

Organism-specific databases

HGNCiHGNC:17351. PRPF18.

Subcellular locationi

Nucleus speckle By similarity
Note: Colocalizes with spliceosomal snRNPs.By similarity

GO - Cellular componenti

  1. nuclear speck Source: UniProtKB-SubCell
  2. nucleus Source: ProtInc
  3. spliceosomal complex Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA38449.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 342342Pre-mRNA-splicing factor 18PRO_0000058582Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ99633.
PaxDbiQ99633.
PRIDEiQ99633.

PTM databases

PhosphoSiteiQ99633.

Expressioni

Gene expression databases

BgeeiQ99633.
CleanExiHS_PRPF18.
ExpressionAtlasiQ99633. baseline and differential.
GenevestigatoriQ99633.

Organism-specific databases

HPAiHPA037599.
HPA047950.

Interactioni

Subunit structurei

Heterodimer with PPIH. Interacts with PRPF4 and with the spliceosome. Part of a complex containing U4/U6 snRNPs.2 Publications

Protein-protein interaction databases

BioGridi114129. 3 interactions.
IntActiQ99633. 1 interaction.
STRINGi9606.ENSP00000367835.

Structurei

Secondary structure

1
342
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi69 – 713Combined sources
Helixi81 – 9111Combined sources
Helixi102 – 11514Combined sources
Beta strandi117 – 1193Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DK4NMR-A60-122[»]
ProteinModelPortaliQ99633.
SMRiQ99633. Positions 63-122.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ99633.

Family & Domainsi

Sequence similaritiesi

Belongs to the PRP18 family.Curated

Phylogenomic databases

eggNOGiNOG313018.
GeneTreeiENSGT00390000015073.
HOGENOMiHOG000242389.
HOVERGENiHBG053696.
InParanoidiQ99633.
KOiK12817.
OMAiRREHELK.
OrthoDBiEOG7034HM.
PhylomeDBiQ99633.
TreeFamiTF315049.

Family and domain databases

Gene3Di1.20.940.10. 1 hit.
InterProiIPR004098. Prp18.
IPR014906. PRP4-like.
IPR003648. SFM.
[Graphical view]
PfamiPF02840. Prp18. 1 hit.
PF08799. PRP4. 1 hit.
[Graphical view]
SMARTiSM00500. SFM. 1 hit.
[Graphical view]
SUPFAMiSSF47938. SSF47938. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q99633-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDILKSEILR KRQLVEDRNL LVENKKYFKR SELAKKEEEA YFERCGYKIQ
60 70 80 90 100
PKEEDQKPLT SSNPVLELEL AEEKLPMTLS RQEVIRRLRE RGEPIRLFGE
110 120 130 140 150
TDYDAFQRLR KIEILTPEVN KGLRNDLKAA LDKIDQQYLN EIVGGQEPGE
160 170 180 190 200
EDTQNDLKVH EENTTIEELE ALGESLGKGD DHKDMDIITK FLKFLLGVWA
210 220 230 240 250
KELNAREDYV KRSVQGKLNS ATQKQTESYL RPLFRKLRKR NLPADIKESI
260 270 280 290 300
TDIIKFMLQR EYVKANDAYL QMAIGNAPWP IGVTMVGIHA RTGREKIFSK
310 320 330 340
HVAHVLNDET QRKYIQGLKR LMTICQKHFP TDPSKCVEYN AL
Length:342
Mass (Da):39,860
Last modified:May 1, 1997 - v1
Checksum:i269D79E1E733CF6D
GO
Isoform 2 (identifier: Q99633-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     22-259: Missing.
     286-342: Missing.

Note: No experimental confirmation available.

Show »
Length:47
Mass (Da):5,474
Checksum:i3A4FB4FF31181C8A
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei22 – 259238Missing in isoform 2. 1 PublicationVSP_008327Add
BLAST
Alternative sequencei286 – 34257Missing in isoform 2. 1 PublicationVSP_008328Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51990 mRNA. Translation: AAB41490.1.
AL157392 Genomic DNA. Translation: CAI13123.1.
CH471072 Genomic DNA. Translation: EAW86281.1.
BC000794 mRNA. Translation: AAH00794.1.
BC002572 mRNA. No translation available.
CCDSiCCDS7100.1. [Q99633-1]
RefSeqiNP_003666.1. NM_003675.3. [Q99633-1]
UniGeneiHs.729158.

Genome annotation databases

EnsembliENST00000378572; ENSP00000367835; ENSG00000165630. [Q99633-1]
GeneIDi8559.
KEGGihsa:8559.
UCSCiuc001imp.3. human. [Q99633-1]

Polymorphism databases

DMDMi37082244.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51990 mRNA. Translation: AAB41490.1.
AL157392 Genomic DNA. Translation: CAI13123.1.
CH471072 Genomic DNA. Translation: EAW86281.1.
BC000794 mRNA. Translation: AAH00794.1.
BC002572 mRNA. No translation available.
CCDSiCCDS7100.1. [Q99633-1]
RefSeqiNP_003666.1. NM_003675.3. [Q99633-1]
UniGeneiHs.729158.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DK4NMR-A60-122[»]
ProteinModelPortaliQ99633.
SMRiQ99633. Positions 63-122.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114129. 3 interactions.
IntActiQ99633. 1 interaction.
STRINGi9606.ENSP00000367835.

PTM databases

PhosphoSiteiQ99633.

Polymorphism databases

DMDMi37082244.

Proteomic databases

MaxQBiQ99633.
PaxDbiQ99633.
PRIDEiQ99633.

Protocols and materials databases

DNASUi8559.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000378572; ENSP00000367835; ENSG00000165630. [Q99633-1]
GeneIDi8559.
KEGGihsa:8559.
UCSCiuc001imp.3. human. [Q99633-1]

Organism-specific databases

CTDi8559.
GeneCardsiGC10P013628.
HGNCiHGNC:17351. PRPF18.
HPAiHPA037599.
HPA047950.
MIMi604993. gene.
neXtProtiNX_Q99633.
PharmGKBiPA38449.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG313018.
GeneTreeiENSGT00390000015073.
HOGENOMiHOG000242389.
HOVERGENiHBG053696.
InParanoidiQ99633.
KOiK12817.
OMAiRREHELK.
OrthoDBiEOG7034HM.
PhylomeDBiQ99633.
TreeFamiTF315049.

Miscellaneous databases

ChiTaRSiPRPF18. human.
EvolutionaryTraceiQ99633.
GenomeRNAii8559.
NextBioi32085.
PROiQ99633.
SOURCEiSearch...

Gene expression databases

BgeeiQ99633.
CleanExiHS_PRPF18.
ExpressionAtlasiQ99633. baseline and differential.
GenevestigatoriQ99633.

Family and domain databases

Gene3Di1.20.940.10. 1 hit.
InterProiIPR004098. Prp18.
IPR014906. PRP4-like.
IPR003648. SFM.
[Graphical view]
PfamiPF02840. Prp18. 1 hit.
PF08799. PRP4. 1 hit.
[Graphical view]
SMARTiSM00500. SFM. 1 hit.
[Graphical view]
SUPFAMiSSF47938. SSF47938. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A human protein required for the second step of pre-mRNA splicing is functionally related to a yeast splicing factor."
    Horowitz D.S., Krainer A.R.
    Genes Dev. 11:139-151(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH PRPF4 AND THE SPLICEOSOME.
    Tissue: Cervix carcinoma.
  2. "The DNA sequence and comparative analysis of human chromosome 10."
    Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J.
    , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
    Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Brain and Placenta.
  5. "A cyclophilin functions in pre-mRNA splicing."
    Horowitz D.S., Lee E.J., Mabon S.A., Misteli T.
    EMBO J. 21:470-480(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PPIH.
  6. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Solution structure of splicing factor motif in pre-mRNA splicing factor 18 (HPRP18)."
    RIKEN structural genomics initiative (RSGI)
    Submitted (OCT-2006) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 60-122.

Entry informationi

Entry nameiPRP18_HUMAN
AccessioniPrimary (citable) accession number: Q99633
Secondary accession number(s): Q5T9P9, Q9BUI9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: May 1, 1997
Last modified: March 4, 2015
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.