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Q99633

- PRP18_HUMAN

UniProt

Q99633 - PRP18_HUMAN

Protein

Pre-mRNA-splicing factor 18

Gene

PRPF18

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
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    Functioni

    Participates in the second step of pre-mRNA splicing.1 Publication

    GO - Molecular functioni

    1. potassium channel inhibitor activity Source: Ensembl

    GO - Biological processi

    1. mRNA processing Source: ProtInc
    2. RNA splicing Source: ProtInc

    Keywords - Biological processi

    mRNA processing, mRNA splicing

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pre-mRNA-splicing factor 18
    Alternative name(s):
    PRP18 homolog
    Short name:
    hPRP18
    Gene namesi
    Name:PRPF18
    Synonyms:HPRP18
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 10

    Organism-specific databases

    HGNCiHGNC:17351. PRPF18.

    Subcellular locationi

    Nucleus speckle By similarity
    Note: Colocalizes with spliceosomal snRNPs.By similarity

    GO - Cellular componenti

    1. nuclear speck Source: UniProtKB-SubCell
    2. nucleus Source: ProtInc
    3. spliceosomal complex Source: ProtInc

    Keywords - Cellular componenti

    Nucleus, Spliceosome

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA38449.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 342342Pre-mRNA-splicing factor 18PRO_0000058582Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ99633.
    PaxDbiQ99633.
    PRIDEiQ99633.

    PTM databases

    PhosphoSiteiQ99633.

    Expressioni

    Gene expression databases

    ArrayExpressiQ99633.
    BgeeiQ99633.
    CleanExiHS_PRPF18.
    GenevestigatoriQ99633.

    Organism-specific databases

    HPAiHPA037599.

    Interactioni

    Subunit structurei

    Heterodimer with PPIH. Interacts with PRPF4 and with the spliceosome. Part of a complex containing U4/U6 snRNPs.2 Publications

    Protein-protein interaction databases

    BioGridi114129. 3 interactions.
    IntActiQ99633. 1 interaction.
    STRINGi9606.ENSP00000367835.

    Structurei

    Secondary structure

    1
    342
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi69 – 713
    Helixi81 – 9111
    Helixi102 – 11514
    Beta strandi117 – 1193

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2DK4NMR-A60-122[»]
    ProteinModelPortaliQ99633.
    SMRiQ99633. Positions 63-122.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ99633.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PRP18 family.Curated

    Phylogenomic databases

    eggNOGiNOG313018.
    HOGENOMiHOG000242389.
    HOVERGENiHBG053696.
    InParanoidiQ99633.
    KOiK12817.
    OMAiYFKRGEL.
    OrthoDBiEOG7034HM.
    PhylomeDBiQ99633.
    TreeFamiTF315049.

    Family and domain databases

    Gene3Di1.20.940.10. 1 hit.
    InterProiIPR004098. Prp18.
    IPR014906. PRP4-like.
    IPR003648. SFM.
    [Graphical view]
    PfamiPF02840. Prp18. 1 hit.
    PF08799. PRP4. 1 hit.
    [Graphical view]
    SMARTiSM00500. SFM. 1 hit.
    [Graphical view]
    SUPFAMiSSF47938. SSF47938. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q99633-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDILKSEILR KRQLVEDRNL LVENKKYFKR SELAKKEEEA YFERCGYKIQ    50
    PKEEDQKPLT SSNPVLELEL AEEKLPMTLS RQEVIRRLRE RGEPIRLFGE 100
    TDYDAFQRLR KIEILTPEVN KGLRNDLKAA LDKIDQQYLN EIVGGQEPGE 150
    EDTQNDLKVH EENTTIEELE ALGESLGKGD DHKDMDIITK FLKFLLGVWA 200
    KELNAREDYV KRSVQGKLNS ATQKQTESYL RPLFRKLRKR NLPADIKESI 250
    TDIIKFMLQR EYVKANDAYL QMAIGNAPWP IGVTMVGIHA RTGREKIFSK 300
    HVAHVLNDET QRKYIQGLKR LMTICQKHFP TDPSKCVEYN AL 342
    Length:342
    Mass (Da):39,860
    Last modified:May 1, 1997 - v1
    Checksum:i269D79E1E733CF6D
    GO
    Isoform 2 (identifier: Q99633-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         22-259: Missing.
         286-342: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:47
    Mass (Da):5,474
    Checksum:i3A4FB4FF31181C8A
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei22 – 259238Missing in isoform 2. 1 PublicationVSP_008327Add
    BLAST
    Alternative sequencei286 – 34257Missing in isoform 2. 1 PublicationVSP_008328Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U51990 mRNA. Translation: AAB41490.1.
    AL157392 Genomic DNA. Translation: CAI13123.1.
    CH471072 Genomic DNA. Translation: EAW86281.1.
    BC000794 mRNA. Translation: AAH00794.1.
    BC002572 mRNA. No translation available.
    CCDSiCCDS7100.1. [Q99633-1]
    RefSeqiNP_003666.1. NM_003675.3. [Q99633-1]
    UniGeneiHs.729158.

    Genome annotation databases

    EnsembliENST00000378572; ENSP00000367835; ENSG00000165630. [Q99633-1]
    GeneIDi8559.
    KEGGihsa:8559.
    UCSCiuc001imp.3. human. [Q99633-1]

    Polymorphism databases

    DMDMi37082244.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U51990 mRNA. Translation: AAB41490.1 .
    AL157392 Genomic DNA. Translation: CAI13123.1 .
    CH471072 Genomic DNA. Translation: EAW86281.1 .
    BC000794 mRNA. Translation: AAH00794.1 .
    BC002572 mRNA. No translation available.
    CCDSi CCDS7100.1. [Q99633-1 ]
    RefSeqi NP_003666.1. NM_003675.3. [Q99633-1 ]
    UniGenei Hs.729158.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2DK4 NMR - A 60-122 [» ]
    ProteinModelPortali Q99633.
    SMRi Q99633. Positions 63-122.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114129. 3 interactions.
    IntActi Q99633. 1 interaction.
    STRINGi 9606.ENSP00000367835.

    PTM databases

    PhosphoSitei Q99633.

    Polymorphism databases

    DMDMi 37082244.

    Proteomic databases

    MaxQBi Q99633.
    PaxDbi Q99633.
    PRIDEi Q99633.

    Protocols and materials databases

    DNASUi 8559.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000378572 ; ENSP00000367835 ; ENSG00000165630 . [Q99633-1 ]
    GeneIDi 8559.
    KEGGi hsa:8559.
    UCSCi uc001imp.3. human. [Q99633-1 ]

    Organism-specific databases

    CTDi 8559.
    GeneCardsi GC10P013628.
    HGNCi HGNC:17351. PRPF18.
    HPAi HPA037599.
    MIMi 604993. gene.
    neXtProti NX_Q99633.
    PharmGKBi PA38449.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG313018.
    HOGENOMi HOG000242389.
    HOVERGENi HBG053696.
    InParanoidi Q99633.
    KOi K12817.
    OMAi YFKRGEL.
    OrthoDBi EOG7034HM.
    PhylomeDBi Q99633.
    TreeFami TF315049.

    Miscellaneous databases

    ChiTaRSi PRPF18. human.
    EvolutionaryTracei Q99633.
    GenomeRNAii 8559.
    NextBioi 32085.
    PROi Q99633.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q99633.
    Bgeei Q99633.
    CleanExi HS_PRPF18.
    Genevestigatori Q99633.

    Family and domain databases

    Gene3Di 1.20.940.10. 1 hit.
    InterProi IPR004098. Prp18.
    IPR014906. PRP4-like.
    IPR003648. SFM.
    [Graphical view ]
    Pfami PF02840. Prp18. 1 hit.
    PF08799. PRP4. 1 hit.
    [Graphical view ]
    SMARTi SM00500. SFM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47938. SSF47938. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "A human protein required for the second step of pre-mRNA splicing is functionally related to a yeast splicing factor."
      Horowitz D.S., Krainer A.R.
      Genes Dev. 11:139-151(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH PRPF4 AND THE SPLICEOSOME.
      Tissue: Cervix carcinoma.
    2. "The DNA sequence and comparative analysis of human chromosome 10."
      Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J.
      , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
      Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Brain and Placenta.
    5. "A cyclophilin functions in pre-mRNA splicing."
      Horowitz D.S., Lee E.J., Mabon S.A., Misteli T.
      EMBO J. 21:470-480(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PPIH.
    6. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "Solution structure of splicing factor motif in pre-mRNA splicing factor 18 (HPRP18)."
      RIKEN structural genomics initiative (RSGI)
      Submitted (OCT-2006) to the PDB data bank
      Cited for: STRUCTURE BY NMR OF 60-122.

    Entry informationi

    Entry nameiPRP18_HUMAN
    AccessioniPrimary (citable) accession number: Q99633
    Secondary accession number(s): Q5T9P9, Q9BUI9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2003
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 121 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 10
      Human chromosome 10: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3