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Q99627

- CSN8_HUMAN

UniProt

Q99627 - CSN8_HUMAN

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Protein

COP9 signalosome complex subunit 8

Gene

COPS8

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.5 Publications

GO - Biological processi

  1. activation of NF-kappaB-inducing kinase activity Source: UniProt
  2. cullin deneddylation Source: UniProtKB
  3. negative regulation of cell proliferation Source: UniProt
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
COP9 signalosome complex subunit 8
Short name:
SGN8
Short name:
Signalosome subunit 8
Alternative name(s):
COP9 homolog
Short name:
hCOP9
JAB1-containing signalosome subunit 8
Gene namesi
Name:COPS8
Synonyms:CSN8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 2

Organism-specific databases

HGNCiHGNC:24335. COPS8.

Subcellular locationi

Cytoplasm 1 Publication. Nucleus 1 Publication

GO - Cellular componenti

  1. COP9 signalosome Source: UniProtKB
  2. cytoplasm Source: HPA
  3. extracellular vesicular exosome Source: UniProt
  4. nucleus Source: HPA
  5. perinuclear region of cytoplasm Source: UniProt
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Signalosome

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134968686.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 209208COP9 signalosome complex subunit 8PRO_0000121007Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei175 – 1751Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ99627.
PaxDbiQ99627.
PRIDEiQ99627.

PTM databases

PhosphoSiteiQ99627.

Expressioni

Gene expression databases

BgeeiQ99627.
CleanExiHS_COPS8.
ExpressionAtlasiQ99627. baseline and differential.
GenevestigatoriQ99627.

Organism-specific databases

HPAiHPA036485.

Interactioni

Subunit structurei

Component of the CSN complex, composed of COPS1/GPS1, COPS2, COPS3, COPS4, COPS5, COP6, COPS7 (COPS7A or COPS7B) and COPS8. In the complex, it probably interacts directly with COPS3, COPS4 and COPS7 (COPS7A or COPS7B).1 Publication

Protein-protein interaction databases

BioGridi116124. 42 interactions.
DIPiDIP-42075N.
IntActiQ99627. 5 interactions.
MINTiMINT-1204089.
STRINGi9606.ENSP00000346340.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4D10X-ray3.80H/P2-209[»]
4D18X-ray4.08H/P1-209[»]
ProteinModelPortaliQ99627.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini68 – 15184PCIAdd
BLAST

Sequence similaritiesi

Belongs to the CSN8 family.Curated
Contains 1 PCI domain.Curated

Phylogenomic databases

eggNOGiNOG321945.
GeneTreeiENSGT00390000000977.
HOGENOMiHOG000253954.
HOVERGENiHBG051139.
InParanoidiQ99627.
KOiK12181.
OMAiISAHQWS.
OrthoDBiEOG76DTV5.
PhylomeDBiQ99627.
TreeFamiTF101150.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q99627-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPVAVMAESA FSFKKLLDQC ENQELEAPGG IATPPVYGQL LALYLLHNDM
60 70 80 90 100
NNARYLWKRI PPAIKSANSE LGGIWSVGQR IWQRDFPGIY TTINAHQWSE
110 120 130 140 150
TVQPIMEALR DATRRRAFAL VSQAYTSIIA DDFAAFVGLP VEEAVKGILE
160 170 180 190 200
QGWQADSTTR MVLPRKPVAG ALDVSFNKFI PLSEPAPVPP IPNEQQLARL

TDYVAFLEN
Length:209
Mass (Da):23,226
Last modified:May 1, 1997 - v1
Checksum:i203E236D8E304A8A
GO
Isoform 2 (identifier: Q99627-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-49: Missing.

Note: No experimental confirmation available.

Show »
Length:160
Mass (Da):17,909
Checksum:i3E7A1CA5C341955A
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 4949Missing in isoform 2. 1 PublicationVSP_042715Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U51205 mRNA. Translation: AAB38529.1.
AK289891 mRNA. Translation: BAF82580.1.
CR456994 mRNA. Translation: CAG33275.1.
AC105760 Genomic DNA. Translation: AAY14978.1.
CH471063 Genomic DNA. Translation: EAW71097.1.
CH471063 Genomic DNA. Translation: EAW71098.1.
BC003090 mRNA. Translation: AAH03090.1.
BC036499 mRNA. Translation: AAH36499.1.
BC080617 mRNA. Translation: AAH80617.1.
CCDSiCCDS2517.1. [Q99627-1]
CCDS42835.1. [Q99627-2]
RefSeqiNP_006701.1. NM_006710.4. [Q99627-1]
NP_937832.1. NM_198189.2. [Q99627-2]
UniGeneiHs.531713.

Genome annotation databases

EnsembliENST00000354371; ENSP00000346340; ENSG00000198612. [Q99627-1]
ENST00000392008; ENSP00000375865; ENSG00000198612. [Q99627-2]
GeneIDi10920.
KEGGihsa:10920.
UCSCiuc002vwg.3. human. [Q99627-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U51205 mRNA. Translation: AAB38529.1 .
AK289891 mRNA. Translation: BAF82580.1 .
CR456994 mRNA. Translation: CAG33275.1 .
AC105760 Genomic DNA. Translation: AAY14978.1 .
CH471063 Genomic DNA. Translation: EAW71097.1 .
CH471063 Genomic DNA. Translation: EAW71098.1 .
BC003090 mRNA. Translation: AAH03090.1 .
BC036499 mRNA. Translation: AAH36499.1 .
BC080617 mRNA. Translation: AAH80617.1 .
CCDSi CCDS2517.1. [Q99627-1 ]
CCDS42835.1. [Q99627-2 ]
RefSeqi NP_006701.1. NM_006710.4. [Q99627-1 ]
NP_937832.1. NM_198189.2. [Q99627-2 ]
UniGenei Hs.531713.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4D10 X-ray 3.80 H/P 2-209 [» ]
4D18 X-ray 4.08 H/P 1-209 [» ]
ProteinModelPortali Q99627.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 116124. 42 interactions.
DIPi DIP-42075N.
IntActi Q99627. 5 interactions.
MINTi MINT-1204089.
STRINGi 9606.ENSP00000346340.

PTM databases

PhosphoSitei Q99627.

Proteomic databases

MaxQBi Q99627.
PaxDbi Q99627.
PRIDEi Q99627.

Protocols and materials databases

DNASUi 10920.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000354371 ; ENSP00000346340 ; ENSG00000198612 . [Q99627-1 ]
ENST00000392008 ; ENSP00000375865 ; ENSG00000198612 . [Q99627-2 ]
GeneIDi 10920.
KEGGi hsa:10920.
UCSCi uc002vwg.3. human. [Q99627-1 ]

Organism-specific databases

CTDi 10920.
GeneCardsi GC02P237993.
HGNCi HGNC:24335. COPS8.
HPAi HPA036485.
neXtProti NX_Q99627.
PharmGKBi PA134968686.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG321945.
GeneTreei ENSGT00390000000977.
HOGENOMi HOG000253954.
HOVERGENi HBG051139.
InParanoidi Q99627.
KOi K12181.
OMAi ISAHQWS.
OrthoDBi EOG76DTV5.
PhylomeDBi Q99627.
TreeFami TF101150.

Miscellaneous databases

ChiTaRSi COPS8. human.
GeneWikii COPS8.
GenomeRNAii 10920.
NextBioi 41481.
PROi Q99627.

Gene expression databases

Bgeei Q99627.
CleanExi HS_COPS8.
ExpressionAtlasi Q99627. baseline and differential.
Genevestigatori Q99627.

Family and domain databases

ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The novel components of the Arabidopsis light signaling pathway may define a group of general developmental regulators shared by both animal and plant kingdoms."
    Chamovitz D.A., Deng X.-W.
    Cell 82:353-354(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Corpus callosum.
  3. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  4. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain and Skin.
  7. "A novel protein complex involved in signal transduction possessing similarities to 26S proteasome subunits."
    Seeger M., Kraft R., Ferrell K., Bech-Otschir D., Dumdey R., Schade R., Gordon C., Naumann M., Dubiel W.
    FASEB J. 12:469-478(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION.
  8. "COP9 signalosome-specific phosphorylation targets p53 to degradation by the ubiquitin system."
    Bech-Otschir D., Kraft R., Huang X., Henklein P., Kapelari B., Pollmann C., Dubiel W.
    EMBO J. 20:1630-1639(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. Cited for: FUNCTION, COMPOSITION OF THE CSN COMPLEX.
  10. "The ubiquitin ligase activity in the DDB2 and CSA complexes is differentially regulated by the COP9 signalosome in response to DNA damage."
    Groisman R., Polanowska J., Kuraoka I., Sawada J., Saijo M., Drapkin R., Kisselev A.F., Tanaka K., Nakatani Y.
    Cell 113:357-367(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Protein kinase CK2 and protein kinase D are associated with the COP9 signalosome."
    Uhle S., Medalia O., Waldron R., Dumdey R., Henklein P., Bech-Otschir D., Huang X., Berse M., Sperling J., Schade R., Dubiel W.
    EMBO J. 22:1302-1312(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "Characterization of the human COP9 signalosome complex using affinity purification and mass spectrometry."
    Fang L., Wang X., Yamoah K., Chen P.L., Pan Z.Q., Huang L.
    J. Proteome Res. 7:4914-4925(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CSN COMPLEX, CLEAVAGE OF INITIATOR METHIONINE, PHOSPHORYLATION AT SER-175.
  13. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  14. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-175, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  15. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCSN8_HUMAN
AccessioniPrimary (citable) accession number: Q99627
Secondary accession number(s): A8K1H6, Q53QS9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: May 1, 1997
Last modified: October 29, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3