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Q99627

- CSN8_HUMAN

UniProt

Q99627 - CSN8_HUMAN

Protein

COP9 signalosome complex subunit 8

Gene

COPS8

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
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    Functioni

    Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.5 Publications

    GO - Biological processi

    1. activation of NF-kappaB-inducing kinase activity Source: UniProt
    2. cullin deneddylation Source: UniProtKB
    3. negative regulation of cell proliferation Source: UniProt

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    COP9 signalosome complex subunit 8
    Short name:
    SGN8
    Short name:
    Signalosome subunit 8
    Alternative name(s):
    COP9 homolog
    Short name:
    hCOP9
    JAB1-containing signalosome subunit 8
    Gene namesi
    Name:COPS8
    Synonyms:CSN8
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 2

    Organism-specific databases

    HGNCiHGNC:24335. COPS8.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. COP9 signalosome Source: UniProtKB
    2. cytoplasm Source: HPA
    3. extracellular vesicular exosome Source: UniProt
    4. nucleus Source: HPA
    5. perinuclear region of cytoplasm Source: UniProt

    Keywords - Cellular componenti

    Cytoplasm, Nucleus, Signalosome

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134968686.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 209208COP9 signalosome complex subunit 8PRO_0000121007Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei175 – 1751Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ99627.
    PaxDbiQ99627.
    PRIDEiQ99627.

    PTM databases

    PhosphoSiteiQ99627.

    Expressioni

    Gene expression databases

    ArrayExpressiQ99627.
    BgeeiQ99627.
    CleanExiHS_COPS8.
    GenevestigatoriQ99627.

    Organism-specific databases

    HPAiHPA036485.

    Interactioni

    Subunit structurei

    Component of the CSN complex, composed of COPS1/GPS1, COPS2, COPS3, COPS4, COPS5, COP6, COPS7 (COPS7A or COPS7B) and COPS8. In the complex, it probably interacts directly with COPS3, COPS4 and COPS7 (COPS7A or COPS7B).1 Publication

    Protein-protein interaction databases

    BioGridi116124. 41 interactions.
    DIPiDIP-42075N.
    IntActiQ99627. 5 interactions.
    MINTiMINT-1204089.
    STRINGi9606.ENSP00000346340.

    Structurei

    3D structure databases

    ProteinModelPortaliQ99627.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini68 – 15184PCIAdd
    BLAST

    Sequence similaritiesi

    Belongs to the CSN8 family.Curated
    Contains 1 PCI domain.Curated

    Phylogenomic databases

    eggNOGiNOG321945.
    HOGENOMiHOG000253954.
    HOVERGENiHBG051139.
    InParanoidiQ99627.
    KOiK12181.
    OMAiISAHQWS.
    OrthoDBiEOG76DTV5.
    PhylomeDBiQ99627.
    TreeFamiTF101150.

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q99627-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MPVAVMAESA FSFKKLLDQC ENQELEAPGG IATPPVYGQL LALYLLHNDM    50
    NNARYLWKRI PPAIKSANSE LGGIWSVGQR IWQRDFPGIY TTINAHQWSE 100
    TVQPIMEALR DATRRRAFAL VSQAYTSIIA DDFAAFVGLP VEEAVKGILE 150
    QGWQADSTTR MVLPRKPVAG ALDVSFNKFI PLSEPAPVPP IPNEQQLARL 200
    TDYVAFLEN 209
    Length:209
    Mass (Da):23,226
    Last modified:May 1, 1997 - v1
    Checksum:i203E236D8E304A8A
    GO
    Isoform 2 (identifier: Q99627-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-49: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:160
    Mass (Da):17,909
    Checksum:i3E7A1CA5C341955A
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 4949Missing in isoform 2. 1 PublicationVSP_042715Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U51205 mRNA. Translation: AAB38529.1.
    AK289891 mRNA. Translation: BAF82580.1.
    CR456994 mRNA. Translation: CAG33275.1.
    AC105760 Genomic DNA. Translation: AAY14978.1.
    CH471063 Genomic DNA. Translation: EAW71097.1.
    CH471063 Genomic DNA. Translation: EAW71098.1.
    BC003090 mRNA. Translation: AAH03090.1.
    BC036499 mRNA. Translation: AAH36499.1.
    BC080617 mRNA. Translation: AAH80617.1.
    CCDSiCCDS2517.1. [Q99627-1]
    CCDS42835.1. [Q99627-2]
    RefSeqiNP_006701.1. NM_006710.4. [Q99627-1]
    NP_937832.1. NM_198189.2. [Q99627-2]
    UniGeneiHs.531713.

    Genome annotation databases

    EnsembliENST00000354371; ENSP00000346340; ENSG00000198612. [Q99627-1]
    ENST00000392008; ENSP00000375865; ENSG00000198612. [Q99627-2]
    GeneIDi10920.
    KEGGihsa:10920.
    UCSCiuc002vwg.3. human. [Q99627-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U51205 mRNA. Translation: AAB38529.1 .
    AK289891 mRNA. Translation: BAF82580.1 .
    CR456994 mRNA. Translation: CAG33275.1 .
    AC105760 Genomic DNA. Translation: AAY14978.1 .
    CH471063 Genomic DNA. Translation: EAW71097.1 .
    CH471063 Genomic DNA. Translation: EAW71098.1 .
    BC003090 mRNA. Translation: AAH03090.1 .
    BC036499 mRNA. Translation: AAH36499.1 .
    BC080617 mRNA. Translation: AAH80617.1 .
    CCDSi CCDS2517.1. [Q99627-1 ]
    CCDS42835.1. [Q99627-2 ]
    RefSeqi NP_006701.1. NM_006710.4. [Q99627-1 ]
    NP_937832.1. NM_198189.2. [Q99627-2 ]
    UniGenei Hs.531713.

    3D structure databases

    ProteinModelPortali Q99627.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 116124. 41 interactions.
    DIPi DIP-42075N.
    IntActi Q99627. 5 interactions.
    MINTi MINT-1204089.
    STRINGi 9606.ENSP00000346340.

    PTM databases

    PhosphoSitei Q99627.

    Proteomic databases

    MaxQBi Q99627.
    PaxDbi Q99627.
    PRIDEi Q99627.

    Protocols and materials databases

    DNASUi 10920.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000354371 ; ENSP00000346340 ; ENSG00000198612 . [Q99627-1 ]
    ENST00000392008 ; ENSP00000375865 ; ENSG00000198612 . [Q99627-2 ]
    GeneIDi 10920.
    KEGGi hsa:10920.
    UCSCi uc002vwg.3. human. [Q99627-1 ]

    Organism-specific databases

    CTDi 10920.
    GeneCardsi GC02P237993.
    HGNCi HGNC:24335. COPS8.
    HPAi HPA036485.
    neXtProti NX_Q99627.
    PharmGKBi PA134968686.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG321945.
    HOGENOMi HOG000253954.
    HOVERGENi HBG051139.
    InParanoidi Q99627.
    KOi K12181.
    OMAi ISAHQWS.
    OrthoDBi EOG76DTV5.
    PhylomeDBi Q99627.
    TreeFami TF101150.

    Miscellaneous databases

    ChiTaRSi COPS8. human.
    GeneWikii COPS8.
    GenomeRNAii 10920.
    NextBioi 41481.
    PROi Q99627.

    Gene expression databases

    ArrayExpressi Q99627.
    Bgeei Q99627.
    CleanExi HS_COPS8.
    Genevestigatori Q99627.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "The novel components of the Arabidopsis light signaling pathway may define a group of general developmental regulators shared by both animal and plant kingdoms."
      Chamovitz D.A., Deng X.-W.
      Cell 82:353-354(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Corpus callosum.
    3. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    4. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
      Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
      , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
      Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain and Skin.
    7. "A novel protein complex involved in signal transduction possessing similarities to 26S proteasome subunits."
      Seeger M., Kraft R., Ferrell K., Bech-Otschir D., Dumdey R., Schade R., Gordon C., Naumann M., Dubiel W.
      FASEB J. 12:469-478(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION.
    8. "COP9 signalosome-specific phosphorylation targets p53 to degradation by the ubiquitin system."
      Bech-Otschir D., Kraft R., Huang X., Henklein P., Kapelari B., Pollmann C., Dubiel W.
      EMBO J. 20:1630-1639(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. Cited for: FUNCTION, COMPOSITION OF THE CSN COMPLEX.
    10. "The ubiquitin ligase activity in the DDB2 and CSA complexes is differentially regulated by the COP9 signalosome in response to DNA damage."
      Groisman R., Polanowska J., Kuraoka I., Sawada J., Saijo M., Drapkin R., Kisselev A.F., Tanaka K., Nakatani Y.
      Cell 113:357-367(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    11. "Protein kinase CK2 and protein kinase D are associated with the COP9 signalosome."
      Uhle S., Medalia O., Waldron R., Dumdey R., Henklein P., Bech-Otschir D., Huang X., Berse M., Sperling J., Schade R., Dubiel W.
      EMBO J. 22:1302-1312(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    12. "Characterization of the human COP9 signalosome complex using affinity purification and mass spectrometry."
      Fang L., Wang X., Yamoah K., Chen P.L., Pan Z.Q., Huang L.
      J. Proteome Res. 7:4914-4925(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE CSN COMPLEX, CLEAVAGE OF INITIATOR METHIONINE, PHOSPHORYLATION AT SER-175.
    13. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    14. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-175, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    15. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCSN8_HUMAN
    AccessioniPrimary (citable) accession number: Q99627
    Secondary accession number(s): A8K1H6, Q53QS9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 23, 2004
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 2
      Human chromosome 2: entries, gene names and cross-references to MIM
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3