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Protein

COP9 signalosome complex subunit 8

Gene

COPS8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.5 Publications

GO - Biological processi

  • activation of NF-kappaB-inducing kinase activity Source: UniProtKB
  • COP9 signalosome assembly Source: InterPro
  • cullin deneddylation Source: UniProtKB
  • negative regulation of cell proliferation Source: UniProtKB
  • nucleotide-excision repair, DNA damage recognition Source: Reactome
  • transcription-coupled nucleotide-excision repair Source: Reactome
Complete GO annotation...

Enzyme and pathway databases

BioCyciZFISH:G66-32314-MONOMER.
ReactomeiR-HSA-5696394. DNA Damage Recognition in GG-NER.
R-HSA-6781823. Formation of TC-NER Pre-Incision Complex.
R-HSA-8856825. Cargo recognition for clathrin-mediated endocytosis.

Names & Taxonomyi

Protein namesi
Recommended name:
COP9 signalosome complex subunit 8
Short name:
SGN8
Short name:
Signalosome subunit 8
Alternative name(s):
COP9 homolog
Short name:
hCOP9
JAB1-containing signalosome subunit 8
Gene namesi
Name:COPS8
Synonyms:CSN8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:24335. COPS8.

Subcellular locationi

GO - Cellular componenti

  • COP9 signalosome Source: UniProtKB
  • cytoplasm Source: HPA
  • extracellular exosome Source: UniProtKB
  • nucleoplasm Source: HPA
  • nucleus Source: ProtInc
  • perinuclear region of cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Signalosome

Pathology & Biotechi

Organism-specific databases

DisGeNETi10920.
OpenTargetsiENSG00000198612.
PharmGKBiPA134968686.

Polymorphism and mutation databases

BioMutaiCOPS8.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001210072 – 209COP9 signalosome complex subunit 8Add BLAST208

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei175PhosphoserineCombined sources1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ99627.
MaxQBiQ99627.
PaxDbiQ99627.
PeptideAtlasiQ99627.
PRIDEiQ99627.
TopDownProteomicsiQ99627-1. [Q99627-1]

PTM databases

iPTMnetiQ99627.
PhosphoSitePlusiQ99627.

Expressioni

Gene expression databases

BgeeiENSG00000198612.
CleanExiHS_COPS8.
ExpressionAtlasiQ99627. baseline and differential.
GenevisibleiQ99627. HS.

Organism-specific databases

HPAiHPA036485.

Interactioni

Subunit structurei

Component of the CSN complex, composed of COPS1/GPS1, COPS2, COPS3, COPS4, COPS5, COPS6, COPS7 (COPS7A or COPS7B), COPS8 and COPS9 isoform 1 (PubMed:11337588, PubMed:18850735, PubMed:26456823). In the complex, it probably interacts directly with COPS3, COPS4 and COPS7 (COPS7A or COPS7B) (PubMed:18850735).3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
COPS3Q9UNS23EBI-2510102,EBI-350590
USHBP1Q8N6Y03EBI-2510102,EBI-739895

Protein-protein interaction databases

BioGridi116124. 58 interactors.
DIPiDIP-42075N.
IntActiQ99627. 18 interactors.
MINTiMINT-1204089.
STRINGi9606.ENSP00000346340.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4D10X-ray3.80H/P2-209[»]
4D18X-ray4.08H/P1-209[»]
4WSNX-ray5.50H/P/X/f/n/v1-209[»]
ProteinModelPortaliQ99627.
SMRiQ99627.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini68 – 151PCIAdd BLAST84

Sequence similaritiesi

Belongs to the CSN8 family.Curated
Contains 1 PCI domain.Curated

Phylogenomic databases

eggNOGiKOG4414. Eukaryota.
ENOG410XW9N. LUCA.
GeneTreeiENSGT00390000000977.
HOGENOMiHOG000253954.
HOVERGENiHBG051139.
InParanoidiQ99627.
KOiK12181.
OMAiDAKLLWM.
OrthoDBiEOG091G0O7O.
PhylomeDBiQ99627.
TreeFamiTF101150.

Family and domain databases

InterProiIPR033205. COP9_CSN8.
IPR033464. CSN8_PSD8_EIF3K.
[Graphical view]
PANTHERiPTHR13339. PTHR13339. 1 hit.
PfamiPF10075. CSN8_PSD8_EIF3K. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q99627-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPVAVMAESA FSFKKLLDQC ENQELEAPGG IATPPVYGQL LALYLLHNDM
60 70 80 90 100
NNARYLWKRI PPAIKSANSE LGGIWSVGQR IWQRDFPGIY TTINAHQWSE
110 120 130 140 150
TVQPIMEALR DATRRRAFAL VSQAYTSIIA DDFAAFVGLP VEEAVKGILE
160 170 180 190 200
QGWQADSTTR MVLPRKPVAG ALDVSFNKFI PLSEPAPVPP IPNEQQLARL

TDYVAFLEN
Length:209
Mass (Da):23,226
Last modified:May 1, 1997 - v1
Checksum:i203E236D8E304A8A
GO
Isoform 2 (identifier: Q99627-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-49: Missing.

Note: No experimental confirmation available.
Show »
Length:160
Mass (Da):17,909
Checksum:i3E7A1CA5C341955A
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0427151 – 49Missing in isoform 2. 1 PublicationAdd BLAST49

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51205 mRNA. Translation: AAB38529.1.
AK289891 mRNA. Translation: BAF82580.1.
CR456994 mRNA. Translation: CAG33275.1.
AC105760 Genomic DNA. Translation: AAY14978.1.
CH471063 Genomic DNA. Translation: EAW71097.1.
CH471063 Genomic DNA. Translation: EAW71098.1.
BC003090 mRNA. Translation: AAH03090.1.
BC036499 mRNA. Translation: AAH36499.1.
BC080617 mRNA. Translation: AAH80617.1.
CCDSiCCDS2517.1. [Q99627-1]
CCDS42835.1. [Q99627-2]
RefSeqiNP_006701.1. NM_006710.4. [Q99627-1]
NP_937832.1. NM_198189.2. [Q99627-2]
UniGeneiHs.531713.

Genome annotation databases

EnsembliENST00000354371; ENSP00000346340; ENSG00000198612. [Q99627-1]
ENST00000392008; ENSP00000375865; ENSG00000198612. [Q99627-2]
GeneIDi10920.
KEGGihsa:10920.
UCSCiuc002vwg.4. human. [Q99627-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51205 mRNA. Translation: AAB38529.1.
AK289891 mRNA. Translation: BAF82580.1.
CR456994 mRNA. Translation: CAG33275.1.
AC105760 Genomic DNA. Translation: AAY14978.1.
CH471063 Genomic DNA. Translation: EAW71097.1.
CH471063 Genomic DNA. Translation: EAW71098.1.
BC003090 mRNA. Translation: AAH03090.1.
BC036499 mRNA. Translation: AAH36499.1.
BC080617 mRNA. Translation: AAH80617.1.
CCDSiCCDS2517.1. [Q99627-1]
CCDS42835.1. [Q99627-2]
RefSeqiNP_006701.1. NM_006710.4. [Q99627-1]
NP_937832.1. NM_198189.2. [Q99627-2]
UniGeneiHs.531713.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4D10X-ray3.80H/P2-209[»]
4D18X-ray4.08H/P1-209[»]
4WSNX-ray5.50H/P/X/f/n/v1-209[»]
ProteinModelPortaliQ99627.
SMRiQ99627.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116124. 58 interactors.
DIPiDIP-42075N.
IntActiQ99627. 18 interactors.
MINTiMINT-1204089.
STRINGi9606.ENSP00000346340.

PTM databases

iPTMnetiQ99627.
PhosphoSitePlusiQ99627.

Polymorphism and mutation databases

BioMutaiCOPS8.

Proteomic databases

EPDiQ99627.
MaxQBiQ99627.
PaxDbiQ99627.
PeptideAtlasiQ99627.
PRIDEiQ99627.
TopDownProteomicsiQ99627-1. [Q99627-1]

Protocols and materials databases

DNASUi10920.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000354371; ENSP00000346340; ENSG00000198612. [Q99627-1]
ENST00000392008; ENSP00000375865; ENSG00000198612. [Q99627-2]
GeneIDi10920.
KEGGihsa:10920.
UCSCiuc002vwg.4. human. [Q99627-1]

Organism-specific databases

CTDi10920.
DisGeNETi10920.
GeneCardsiCOPS8.
HGNCiHGNC:24335. COPS8.
HPAiHPA036485.
MIMi616011. gene.
neXtProtiNX_Q99627.
OpenTargetsiENSG00000198612.
PharmGKBiPA134968686.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4414. Eukaryota.
ENOG410XW9N. LUCA.
GeneTreeiENSGT00390000000977.
HOGENOMiHOG000253954.
HOVERGENiHBG051139.
InParanoidiQ99627.
KOiK12181.
OMAiDAKLLWM.
OrthoDBiEOG091G0O7O.
PhylomeDBiQ99627.
TreeFamiTF101150.

Enzyme and pathway databases

BioCyciZFISH:G66-32314-MONOMER.
ReactomeiR-HSA-5696394. DNA Damage Recognition in GG-NER.
R-HSA-6781823. Formation of TC-NER Pre-Incision Complex.
R-HSA-8856825. Cargo recognition for clathrin-mediated endocytosis.

Miscellaneous databases

ChiTaRSiCOPS8. human.
GeneWikiiCOPS8.
GenomeRNAii10920.
PROiQ99627.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000198612.
CleanExiHS_COPS8.
ExpressionAtlasiQ99627. baseline and differential.
GenevisibleiQ99627. HS.

Family and domain databases

InterProiIPR033205. COP9_CSN8.
IPR033464. CSN8_PSD8_EIF3K.
[Graphical view]
PANTHERiPTHR13339. PTHR13339. 1 hit.
PfamiPF10075. CSN8_PSD8_EIF3K. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCSN8_HUMAN
AccessioniPrimary (citable) accession number: Q99627
Secondary accession number(s): A8K1H6, Q53QS9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: May 1, 1997
Last modified: November 30, 2016
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.