Q99624 (S38A3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sodium-coupled neutral amino acid transporter 3

Alternative name(s):
N-system amino acid transporter 1
Na(+)-coupled neutral amino acid transporter 3
Solute carrier family 38 member 3
System N amino acid transporter 1

Gene names

Name:	SLC38A3
Synonyms:	G17, NAT1, SN1, SNAT3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Sodium-dependent amino acid/proton antiporter. Mediates electrogenic cotransport of glutamine and sodium ions in exchange for protons. Also recognizes histidine, asparagine and alanine. May mediate amino acid transport in either direction under physiological conditions. May play a role in nitrogen metabolism and synaptic transmission. Ref.1 UniProtKB Q9JHZ9
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the amino acid/polyamine transporter 2 family.

Ontologies

Keywords
Biological process	Amino-acid transport Antiport Ion transport Sodium transport Symport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Sodium
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	sodium ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	L-alanine transmembrane transporter activity Inferred from direct assay Ref.1. Source: UniProtKB L-asparagine transmembrane transporter activity Inferred from direct assay Ref.1. Source: UniProtKB L-glutamine transmembrane transporter activity Inferred from direct assay Ref.1. Source: UniProtKB L-histidine transmembrane transporter activity Inferred from direct assay Ref.1. Source: UniProtKB antiporter activity Inferred from electronic annotation. Source: UniProtKB-KW symporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 504	504	Sodium-coupled neutral amino acid transporter 3		PRO_0000093828
Regions
	Transmembrane	83 – 103	21	Helical; Potential
	Transmembrane	106 – 126	21	Helical; Potential
	Transmembrane	144 – 164	21	Helical; Potential
	Transmembrane	187 – 207	21	Helical; Potential
	Transmembrane	213 – 233	21	Helical; Potential
	Transmembrane	324 – 344	21	Helical; Potential
	Transmembrane	366 – 386	21	Helical; Potential
	Transmembrane	408 – 428	21	Helical; Potential
	Transmembrane	431 – 451	21	Helical; Potential
	Transmembrane	471 – 491	21	Helical; Potential
Amino acid modifications
	Modified residue	52	1	Phosphoserine By similarity
	Modified residue	54	1	Phosphoserine By similarity
	Glycosylation	74	1	N-linked (GlcNAc...) Potential
	Glycosylation	247	1	N-linked (GlcNAc...) Potential
	Glycosylation	248	1	N-linked (GlcNAc...) Potential
	Glycosylation	252	1	N-linked (GlcNAc...) Potential
	Glycosylation	323	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	12	1	L → P in CAG33251. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q99624 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 86E7D205C6001C60	FASTA	504	55,773
	10 20 30 40 50 60 MEAPLQTEMV ELVPNGKHSE GLLPVITPMA GNQRVEDPAR SCMEGKSFLQ KSPSKEPHFT 70 80 90 100 110 120 DFEGKTSFGM SVFNLSNAIM GSGILGLAYA MANTGIILFL FLLTAVALLS SYSIHLLLKS 130 140 150 160 170 180 SGVVGIRAYE QLGYRAFGTP GKLAAALAIT LQNIGAMSSY LYIIKSELPL VIQTFLNLEE 190 200 210 220 230 240 KTSDWYMNGN YLVILVSVTI ILPLALMRQL GYLGYSSGFS LSCMVFFLIA VIYKKFHVPC 250 260 270 280 290 300 PLPPNFNNTT GNFSHVEIVK EKVQLQVEPE ASAFCTPSYF TLNSQTAYTI PIMAFAFVCH 310 320 330 340 350 360 PEVLPIYTEL KDPSKKKMQH ISNLSIAVMY IMYFLAALFG YLTFYNGVES ELLHTYSKVD 370 380 390 400 410 420 PFDVLILCVR VAVLTAVTLT VPIVLFPVRR AIQQMLFPNQ EFSWLRHVLI AVGLLTCINL 430 440 450 460 470 480 LVIFAPNILG IFGVIGATSA PFLIFIFPAI FYFRIMPTEK EPARSTPKIL ALCFAMLGFL 490 500 LMTMSLSFII IDWASGTSRH GGNH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary structure, genomic organization, and functional and electrogenic characteristics of human system N 1, a Na+- and H+-coupled glutamine transporter." Fei Y.-J., Sugawara M., Nakanishi T., Huang W., Wang H., Prasad P.D., Leibach F.H., Ganapathy V. J. Biol. Chem. 275:23707-23717(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION. Tissue: Liver.
[2]	Latif F., Lerman M., Minna J., Duh F.-M., Koonin E., Bader S. Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Corpus callosum.
[5]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Retina.
[6]	"The DNA sequence, annotation and analysis of human chromosome 3." Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A. Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[9]	"The 630-kb lung cancer homozygous deletion region on human chromosome 3p21.3: identification and evaluation of the resident candidate tumor suppressor genes." The international lung cancer chromosome 3p21.3 tumor suppressor gene consortium Lerman M.I., Minna J.D. Cancer Res. 60:6116-6133(2000) [PubMed] [Europe PMC] [Abstract] Cited for: DISCUSSION OF SEQUENCE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF244548 mRNA. Translation: AAG15313.1. U49082 mRNA. Translation: AAB47236.1. CR456970 mRNA. Translation: CAG33251.1. AK313461 mRNA. Translation: BAG36247.1. BX537382 mRNA. Translation: CAD97624.1. AC002077 Genomic DNA. No translation available. CH471055 Genomic DNA. Translation: EAW65050.1. BC042875 mRNA. Translation: AAH42875.1.
IPI	IPI00186295.
RefSeq	NP_006832.1. NM_006841.4.
UniGene	Hs.76460.
3D structure databases
ProteinModelPortal	Q99624.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q99624. 3 interactions.
MINT	MINT-1384272.
Protein family/group databases
TCDB	2.A.18.6.3. amino acid/auxin permease (AAAP) family.
PTM databases
PhosphoSite	Q99624.
Polymorphism databases
DMDM	52783419.
Proteomic databases
PRIDE	Q99624.
Protocols and materials databases
DNASU	10991.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	10991.
KEGG	hsa:10991.
UCSC	uc003cyn.4. human.
Organism-specific databases
CTD	10991.
GeneCards	GC03P050244.
HGNC	HGNC:18044. SLC38A3.
MIM	604437. gene.
neXtProt	NX_Q99624.
PharmGKB	PA38281.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	HBG059571.
InParanoid	Q99624.
KO	K13576.
Enzyme and pathway databases
Reactome	REACT_15518. Transmembrane transport of small molecules. REACT_19419. Amino acid and oligopeptide SLC transporters.
Gene expression databases
CleanEx	HS_NAT1. HS_SLC38A3.
Genevestigator	Q99624.
GermOnline	ENSG00000188338. Homo sapiens.
Family and domain databases
InterPro	IPR013057. AA_transpt_TM. [Graphical view]
Pfam	PF01490. Aa_trans. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SLC38A3. human.
DrugBank	DB00174. L-Asparagine. DB00130. L-Glutamine. DB00117. L-Histidine.
GenomeRNAi	10991.
NextBio	41767.
SOURCE	Search...

Entry information

Entry name

S38A3_HUMAN

Accession

Primary (citable) accession number: Q99624
Secondary accession number(s): B2R8Q0, Q6IB34

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	May 1, 1997
Last modified:	April 3, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.