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Protein

Sodium-coupled neutral amino acid transporter 3

Gene

SLC38A3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sodium-dependent amino acid/proton antiporter. Mediates electrogenic cotransport of glutamine and sodium ions in exchange for protons. Also recognizes histidine, asparagine and alanine. May mediate amino acid transport in either direction under physiological conditions. May play a role in nitrogen metabolism and synaptic transmission.By similarity1 Publication

GO - Molecular functioni

  1. antiporter activity Source: UniProtKB-KW
  2. L-alanine transmembrane transporter activity Source: UniProtKB
  3. L-asparagine transmembrane transporter activity Source: UniProtKB
  4. L-glutamine transmembrane transporter activity Source: UniProtKB
  5. L-histidine transmembrane transporter activity Source: UniProtKB
  6. symporter activity Source: UniProtKB-KW

GO - Biological processi

  1. amino acid transport Source: Reactome
  2. asparagine transport Source: UniProtKB
  3. glutamine transport Source: UniProtKB
  4. histidine transport Source: UniProtKB
  5. ion transport Source: Reactome
  6. L-alanine transport Source: UniProtKB
  7. L-histidine transmembrane transport Source: GOC
  8. sodium ion transport Source: UniProtKB-KW
  9. transmembrane transport Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Antiport, Ion transport, Sodium transport, Symport, Transport

Keywords - Ligandi

Sodium

Enzyme and pathway databases

ReactomeiREACT_13796. Amino acid transport across the plasma membrane.

Protein family/group databases

TCDBi2.A.18.6.3. the amino acid/auxin permease (aaap) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium-coupled neutral amino acid transporter 3
Alternative name(s):
N-system amino acid transporter 1
Na(+)-coupled neutral amino acid transporter 3
Solute carrier family 38 member 3
System N amino acid transporter 1
Gene namesi
Name:SLC38A3Imported
Synonyms:G17, NAT1, SN1, SNAT3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 3

Organism-specific databases

HGNCiHGNC:18044. SLC38A3.

Subcellular locationi

Cell membrane By similarity; Multi-pass membrane protein By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei83 – 10321HelicalSequence AnalysisAdd
BLAST
Transmembranei106 – 12621HelicalSequence AnalysisAdd
BLAST
Transmembranei144 – 16421HelicalSequence AnalysisAdd
BLAST
Transmembranei187 – 20721HelicalSequence AnalysisAdd
BLAST
Transmembranei213 – 23321HelicalSequence AnalysisAdd
BLAST
Transmembranei324 – 34421HelicalSequence AnalysisAdd
BLAST
Transmembranei366 – 38621HelicalSequence AnalysisAdd
BLAST
Transmembranei408 – 42821HelicalSequence AnalysisAdd
BLAST
Transmembranei431 – 45121HelicalSequence AnalysisAdd
BLAST
Transmembranei471 – 49121HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of plasma membrane Source: UniProtKB
  2. plasma membrane Source: LIFEdb
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA38281.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 504504Sodium-coupled neutral amino acid transporter 3PRO_0000093828Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi74 – 741N-linked (GlcNAc...)Sequence Analysis
Glycosylationi247 – 2471N-linked (GlcNAc...)Sequence Analysis
Glycosylationi248 – 2481N-linked (GlcNAc...)Sequence Analysis
Glycosylationi252 – 2521N-linked (GlcNAc...)Sequence Analysis
Glycosylationi323 – 3231N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ99624.
PRIDEiQ99624.

PTM databases

PhosphoSiteiQ99624.

Expressioni

Gene expression databases

CleanExiHS_NAT1.
HS_SLC38A3.
GenevestigatoriQ99624.

Interactioni

Protein-protein interaction databases

BioGridi116187. 4 interactions.
IntActiQ99624. 3 interactions.
MINTiMINT-1384272.

Structurei

3D structure databases

ProteinModelPortaliQ99624.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00760000119147.
HOVERGENiHBG059571.
InParanoidiQ99624.
KOiK13576.
PhylomeDBiQ99624.

Family and domain databases

InterProiIPR013057. AA_transpt_TM.
[Graphical view]
PfamiPF01490. Aa_trans. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99624-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAPLQTEMV ELVPNGKHSE GLLPVITPMA GNQRVEDPAR SCMEGKSFLQ
60 70 80 90 100
KSPSKEPHFT DFEGKTSFGM SVFNLSNAIM GSGILGLAYA MANTGIILFL
110 120 130 140 150
FLLTAVALLS SYSIHLLLKS SGVVGIRAYE QLGYRAFGTP GKLAAALAIT
160 170 180 190 200
LQNIGAMSSY LYIIKSELPL VIQTFLNLEE KTSDWYMNGN YLVILVSVTI
210 220 230 240 250
ILPLALMRQL GYLGYSSGFS LSCMVFFLIA VIYKKFHVPC PLPPNFNNTT
260 270 280 290 300
GNFSHVEIVK EKVQLQVEPE ASAFCTPSYF TLNSQTAYTI PIMAFAFVCH
310 320 330 340 350
PEVLPIYTEL KDPSKKKMQH ISNLSIAVMY IMYFLAALFG YLTFYNGVES
360 370 380 390 400
ELLHTYSKVD PFDVLILCVR VAVLTAVTLT VPIVLFPVRR AIQQMLFPNQ
410 420 430 440 450
EFSWLRHVLI AVGLLTCINL LVIFAPNILG IFGVIGATSA PFLIFIFPAI
460 470 480 490 500
FYFRIMPTEK EPARSTPKIL ALCFAMLGFL LMTMSLSFII IDWASGTSRH

GGNH
Length:504
Mass (Da):55,773
Last modified:May 1, 1997 - v1
Checksum:i86E7D205C6001C60
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti12 – 121L → P in CAG33251 (Ref. 3) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF244548 mRNA. Translation: AAG15313.1.
U49082 mRNA. Translation: AAB47236.1.
CR456970 mRNA. Translation: CAG33251.1.
AK313461 mRNA. Translation: BAG36247.1.
BX537382 mRNA. Translation: CAD97624.1.
AC002077 Genomic DNA. No translation available.
CH471055 Genomic DNA. Translation: EAW65050.1.
BC042875 mRNA. Translation: AAH42875.1.
CCDSiCCDS74940.1.
RefSeqiNP_006832.1. NM_006841.5.
XP_006713017.1. XM_006712954.1.
UniGeneiHs.76460.

Genome annotation databases

EnsembliENST00000614032; ENSP00000481301; ENSG00000188338.
GeneIDi10991.
KEGGihsa:10991.
UCSCiuc003cyn.4. human.

Polymorphism databases

DMDMi52783419.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF244548 mRNA. Translation: AAG15313.1.
U49082 mRNA. Translation: AAB47236.1.
CR456970 mRNA. Translation: CAG33251.1.
AK313461 mRNA. Translation: BAG36247.1.
BX537382 mRNA. Translation: CAD97624.1.
AC002077 Genomic DNA. No translation available.
CH471055 Genomic DNA. Translation: EAW65050.1.
BC042875 mRNA. Translation: AAH42875.1.
CCDSiCCDS74940.1.
RefSeqiNP_006832.1. NM_006841.5.
XP_006713017.1. XM_006712954.1.
UniGeneiHs.76460.

3D structure databases

ProteinModelPortaliQ99624.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116187. 4 interactions.
IntActiQ99624. 3 interactions.
MINTiMINT-1384272.

Chemistry

DrugBankiDB00174. L-Asparagine.
DB00130. L-Glutamine.
DB00117. L-Histidine.
GuidetoPHARMACOLOGYi1172.

Protein family/group databases

TCDBi2.A.18.6.3. the amino acid/auxin permease (aaap) family.

PTM databases

PhosphoSiteiQ99624.

Polymorphism databases

DMDMi52783419.

Proteomic databases

MaxQBiQ99624.
PRIDEiQ99624.

Protocols and materials databases

DNASUi10991.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000614032; ENSP00000481301; ENSG00000188338.
GeneIDi10991.
KEGGihsa:10991.
UCSCiuc003cyn.4. human.

Organism-specific databases

CTDi10991.
GeneCardsiGC03P050244.
HGNCiHGNC:18044. SLC38A3.
MIMi604437. gene.
neXtProtiNX_Q99624.
PharmGKBiPA38281.
GenAtlasiSearch...

Phylogenomic databases

GeneTreeiENSGT00760000119147.
HOVERGENiHBG059571.
InParanoidiQ99624.
KOiK13576.
PhylomeDBiQ99624.

Enzyme and pathway databases

ReactomeiREACT_13796. Amino acid transport across the plasma membrane.

Miscellaneous databases

ChiTaRSiSLC38A3. human.
GeneWikiiSLC38A3.
GenomeRNAii10991.
NextBioi41767.
PROiQ99624.
SOURCEiSearch...

Gene expression databases

CleanExiHS_NAT1.
HS_SLC38A3.
GenevestigatoriQ99624.

Family and domain databases

InterProiIPR013057. AA_transpt_TM.
[Graphical view]
PfamiPF01490. Aa_trans. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Primary structure, genomic organization, and functional and electrogenic characteristics of human system N 1, a Na+- and H+-coupled glutamine transporter."
    Fei Y.-J., Sugawara M., Nakanishi T., Huang W., Wang H., Prasad P.D., Leibach F.H., Ganapathy V.
    J. Biol. Chem. 275:23707-23717(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    Tissue: LiverImported.
  2. Latif F., Lerman M., Minna J., Duh F.-M., Koonin E., Bader S.
    Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Corpus callosum.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Retina.
  6. "The DNA sequence, annotation and analysis of human chromosome 3."
    Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J.
    , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
    Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: BrainImported.
  9. "The 630-kb lung cancer homozygous deletion region on human chromosome 3p21.3: identification and evaluation of the resident candidate tumor suppressor genes."
    The international lung cancer chromosome 3p21.3 tumor suppressor gene consortium
    Lerman M.I., Minna J.D.
    Cancer Res. 60:6116-6133(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISCUSSION OF SEQUENCE.
  10. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiS38A3_HUMAN
AccessioniPrimary (citable) accession number: Q99624
Secondary accession number(s): B2R8Q0, Q6IB34
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: May 1, 1997
Last modified: March 4, 2015
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.