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Protein

Cell division cycle-associated protein 3

Gene

CDCA3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

F-box-like protein which is required for entry into mitosis. Acts by participating in E3 ligase complexes that mediate the ubiquitination and degradation of WEE1 kinase at G2/M phase (By similarity).By similarity

Pathway:iprotein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division cycle-associated protein 3
Alternative name(s):
Gene-rich cluster protein C8
Trigger of mitotic entry protein 1
Short name:
TOME-1
Gene namesi
Name:CDCA3
Synonyms:C8, GRCC8, TOME1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:14624. CDCA3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA26276.

Polymorphism and mutation databases

BioMutaiCDCA3.
DMDMi74732787.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 268268Cell division cycle-associated protein 3PRO_0000287708Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei29 – 291Phosphoserine2 Publications
Modified residuei31 – 311Phosphoserine1 Publication
Modified residuei37 – 371Phosphothreonine1 Publication
Modified residuei44 – 441Phosphoserine1 Publication
Modified residuei64 – 641Phosphoserine1 Publication
Modified residuei68 – 681PhosphoserineBy similarity
Modified residuei76 – 761Phosphothreonine1 Publication
Modified residuei87 – 871Phosphoserine2 Publications
Modified residuei199 – 1991Phosphoserine6 Publications
Modified residuei202 – 2021Phosphothreonine1 Publication
Modified residuei209 – 2091Phosphoserine5 Publications

Post-translational modificationi

Ubiquitinated and degraded by the APC/C-Cdh1 complex.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ99618.
PaxDbiQ99618.
PRIDEiQ99618.

PTM databases

PhosphoSiteiQ99618.

Expressioni

Gene expression databases

BgeeiQ99618.
CleanExiHS_CDCA3.
ExpressionAtlasiQ99618. baseline and differential.
GenevisibleiQ99618. HS.

Organism-specific databases

HPAiHPA026587.

Interactioni

Subunit structurei

Interacts with SKP1. Part of a SCF (SKP1-cullin-F-box) protein ligase complex (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
CTDSP1Q9GZU74EBI-739534,EBI-751587
CTDSP2O145955EBI-739534,EBI-2802973
TRAF1Q130773EBI-739534,EBI-359224
TRAF2Q129334EBI-739534,EBI-355744

Protein-protein interaction databases

BioGridi123657. 17 interactions.
IntActiQ99618. 6 interactions.
MINTiMINT-1441851.
STRINGi9606.ENSP00000442068.

Structurei

3D structure databases

ProteinModelPortaliQ99618.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni91 – 12030F-box-likeAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi258 – 2603KEN box

Domaini

The KEN box is required for the association with the APC/C-Cdh1 complex.By similarity

Phylogenomic databases

eggNOGiNOG46427.
GeneTreeiENSGT00390000017343.
HOGENOMiHOG000013100.
HOVERGENiHBG053225.
InParanoidiQ99618.
OMAiGSMRNRW.
PhylomeDBiQ99618.
TreeFamiTF101068.

Sequencei

Sequence statusi: Complete.

Q99618-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSAKSVPVT PARPPPHNKH LARVADPRSP SAGILRTPIQ VESSPQPGLP
60 70 80 90 100
AGEQLEGLKH AQDSDPRSPT LGIARTPMKT SSGDPPSPLV KQLSEVFETE
110 120 130 140 150
DSKSNLPPEP VLPPEAPLSS ELDLPLGTQL SVEEQMPPWN QTEFPSKQVF
160 170 180 190 200
SKEEARQPTE TPVASQSSDK PSRDPETPRS SGSMRNRWKP NSSKVLGRSP
210 220 230 240 250
LTILQDDNSP GTLTLRQGKR PSPLSENVSE LKEGAILGTG RLLKTGGRAW
260
EQGQDHDKEN QHFPLVES
Length:268
Mass (Da):28,998
Last modified:May 1, 1997 - v1
Checksum:i48933FA7FF57112C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BG354576 mRNA. No translation available.
U47924 Genomic DNA. Translation: AAB51327.1.
AK291421 mRNA. Translation: BAF84110.1.
CH471116 Genomic DNA. Translation: EAW88728.1.
CH471116 Genomic DNA. Translation: EAW88729.1.
BC002551 mRNA. Translation: AAH02551.1.
BC036512 mRNA. Translation: AAH36512.1.
CCDSiCCDS8565.1.
RefSeqiNP_001284531.1. NM_001297602.1.
NP_001284532.1. NM_001297603.1.
NP_001284533.1. NM_001297604.1.
NP_112589.1. NM_031299.5.
XP_011519329.1. XM_011521027.1.
UniGeneiHs.524216.
Hs.709355.

Genome annotation databases

EnsembliENST00000535406; ENSP00000446339; ENSG00000111665.
ENST00000538862; ENSP00000442068; ENSG00000111665.
GeneIDi83461.
KEGGihsa:83461.
UCSCiuc001qrg.2. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BG354576 mRNA. No translation available.
U47924 Genomic DNA. Translation: AAB51327.1.
AK291421 mRNA. Translation: BAF84110.1.
CH471116 Genomic DNA. Translation: EAW88728.1.
CH471116 Genomic DNA. Translation: EAW88729.1.
BC002551 mRNA. Translation: AAH02551.1.
BC036512 mRNA. Translation: AAH36512.1.
CCDSiCCDS8565.1.
RefSeqiNP_001284531.1. NM_001297602.1.
NP_001284532.1. NM_001297603.1.
NP_001284533.1. NM_001297604.1.
NP_112589.1. NM_031299.5.
XP_011519329.1. XM_011521027.1.
UniGeneiHs.524216.
Hs.709355.

3D structure databases

ProteinModelPortaliQ99618.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123657. 17 interactions.
IntActiQ99618. 6 interactions.
MINTiMINT-1441851.
STRINGi9606.ENSP00000442068.

PTM databases

PhosphoSiteiQ99618.

Polymorphism and mutation databases

BioMutaiCDCA3.
DMDMi74732787.

Proteomic databases

MaxQBiQ99618.
PaxDbiQ99618.
PRIDEiQ99618.

Protocols and materials databases

DNASUi83461.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000535406; ENSP00000446339; ENSG00000111665.
ENST00000538862; ENSP00000442068; ENSG00000111665.
GeneIDi83461.
KEGGihsa:83461.
UCSCiuc001qrg.2. human.

Organism-specific databases

CTDi83461.
GeneCardsiGC12M006960.
HGNCiHGNC:14624. CDCA3.
HPAiHPA026587.
MIMi607749. gene.
neXtProtiNX_Q99618.
PharmGKBiPA26276.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG46427.
GeneTreeiENSGT00390000017343.
HOGENOMiHOG000013100.
HOVERGENiHBG053225.
InParanoidiQ99618.
OMAiGSMRNRW.
PhylomeDBiQ99618.
TreeFamiTF101068.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

GeneWikiiCDCA3.
GenomeRNAii83461.
NextBioi72385.
PROiQ99618.
SOURCEiSearch...

Gene expression databases

BgeeiQ99618.
CleanExiHS_CDCA3.
ExpressionAtlasiQ99618. baseline and differential.
GenevisibleiQ99618. HS.

Family and domain databases

ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Drug target discovery by gene expression analysis: cell cycle genes."
    Walker M.G.
    Curr. Cancer Drug Targets 1:73-83(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Large-scale sequencing in human chromosome 12p13: experimental and computational gene structure determination."
    Ansari-Lari M.A., Shen Y., Muzny D.M., Lee W., Gibbs R.A.
    Genome Res. 7:268-280(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Brain.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Placenta and Testis.
  6. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
    Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
    Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199 AND SER-209, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
    Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
    J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199; THR-202 AND SER-209, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
    Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
    Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199 AND SER-209, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-29; SER-31; THR-37; SER-44; SER-64; THR-76; SER-87; SER-199 AND SER-209, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  10. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-87 AND SER-199, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  12. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-29; SER-199 AND SER-209, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.

Entry informationi

Entry nameiCDCA3_HUMAN
AccessioniPrimary (citable) accession number: Q99618
Secondary accession number(s): A8K5V6, D3DUS6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: May 1, 1997
Last modified: July 22, 2015
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.