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Q99618

- CDCA3_HUMAN

UniProt

Q99618 - CDCA3_HUMAN

Protein

Cell division cycle-associated protein 3

Gene

CDCA3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
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    Functioni

    F-box-like protein which is required for entry into mitosis. Acts by participating in E3 ligase complexes that mediate the ubiquitination and degradation of WEE1 kinase at G2/M phase By similarity.By similarity

    Pathwayi

    GO - Biological processi

    1. mitotic nuclear division Source: UniProtKB-KW
    2. protein ubiquitination Source: UniProtKB-UniPathway

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis, Ubl conjugation pathway

    Enzyme and pathway databases

    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cell division cycle-associated protein 3
    Alternative name(s):
    Gene-rich cluster protein C8
    Trigger of mitotic entry protein 1
    Short name:
    TOME-1
    Gene namesi
    Name:CDCA3
    Synonyms:C8, GRCC8, TOME1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 12

    Organism-specific databases

    HGNCiHGNC:14624. CDCA3.

    Subcellular locationi

    Cytoplasmcytosol By similarity

    GO - Cellular componenti

    1. cytosol Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA26276.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 268268Cell division cycle-associated protein 3PRO_0000287708Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei29 – 291Phosphoserine2 Publications
    Modified residuei31 – 311Phosphoserine1 Publication
    Modified residuei37 – 371Phosphothreonine1 Publication
    Modified residuei44 – 441Phosphoserine1 Publication
    Modified residuei64 – 641Phosphoserine1 Publication
    Modified residuei68 – 681PhosphoserineBy similarity
    Modified residuei76 – 761Phosphothreonine1 Publication
    Modified residuei87 – 871Phosphoserine2 Publications
    Modified residuei94 – 941Phosphoserine2 Publications
    Modified residuei199 – 1991Phosphoserine6 Publications
    Modified residuei202 – 2021Phosphothreonine1 Publication
    Modified residuei209 – 2091Phosphoserine5 Publications

    Post-translational modificationi

    Ubiquitinated and degraded by the APC/C-Cdh1 complex.By similarity

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiQ99618.
    PaxDbiQ99618.
    PRIDEiQ99618.

    PTM databases

    PhosphoSiteiQ99618.

    Expressioni

    Gene expression databases

    ArrayExpressiQ99618.
    BgeeiQ99618.
    CleanExiHS_CDCA3.
    GenevestigatoriQ99618.

    Organism-specific databases

    HPAiHPA026587.

    Interactioni

    Subunit structurei

    Interacts with SKP1. Part of a SCF (SKP1-cullin-F-box) protein ligase complex By similarity.By similarity

    Protein-protein interaction databases

    BioGridi123657. 10 interactions.
    IntActiQ99618. 4 interactions.
    MINTiMINT-1441851.
    STRINGi9606.ENSP00000229265.

    Structurei

    3D structure databases

    ProteinModelPortaliQ99618.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni91 – 12030F-box-likeAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi258 – 2603KEN box

    Domaini

    The KEN box is required for the association with the APC/C-Cdh1 complex.By similarity

    Phylogenomic databases

    eggNOGiNOG46427.
    HOGENOMiHOG000013100.
    HOVERGENiHBG053225.
    InParanoidiQ99618.
    OMAiKTGGRAW.
    PhylomeDBiQ99618.
    TreeFamiTF101068.

    Sequencei

    Sequence statusi: Complete.

    Q99618-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGSAKSVPVT PARPPPHNKH LARVADPRSP SAGILRTPIQ VESSPQPGLP    50
    AGEQLEGLKH AQDSDPRSPT LGIARTPMKT SSGDPPSPLV KQLSEVFETE 100
    DSKSNLPPEP VLPPEAPLSS ELDLPLGTQL SVEEQMPPWN QTEFPSKQVF 150
    SKEEARQPTE TPVASQSSDK PSRDPETPRS SGSMRNRWKP NSSKVLGRSP 200
    LTILQDDNSP GTLTLRQGKR PSPLSENVSE LKEGAILGTG RLLKTGGRAW 250
    EQGQDHDKEN QHFPLVES 268
    Length:268
    Mass (Da):28,998
    Last modified:May 1, 1997 - v1
    Checksum:i48933FA7FF57112C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BG354576 mRNA. No translation available.
    U47924 Genomic DNA. Translation: AAB51327.1.
    AK291421 mRNA. Translation: BAF84110.1.
    CH471116 Genomic DNA. Translation: EAW88728.1.
    CH471116 Genomic DNA. Translation: EAW88729.1.
    BC002551 mRNA. Translation: AAH02551.1.
    BC036512 mRNA. Translation: AAH36512.1.
    CCDSiCCDS8565.1.
    RefSeqiNP_112589.1. NM_031299.4.
    UniGeneiHs.524216.

    Genome annotation databases

    EnsembliENST00000535406; ENSP00000446339; ENSG00000111665.
    ENST00000538862; ENSP00000442068; ENSG00000111665.
    GeneIDi83461.
    KEGGihsa:83461.
    UCSCiuc001qrg.2. human.

    Polymorphism databases

    DMDMi74732787.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BG354576 mRNA. No translation available.
    U47924 Genomic DNA. Translation: AAB51327.1 .
    AK291421 mRNA. Translation: BAF84110.1 .
    CH471116 Genomic DNA. Translation: EAW88728.1 .
    CH471116 Genomic DNA. Translation: EAW88729.1 .
    BC002551 mRNA. Translation: AAH02551.1 .
    BC036512 mRNA. Translation: AAH36512.1 .
    CCDSi CCDS8565.1.
    RefSeqi NP_112589.1. NM_031299.4.
    UniGenei Hs.524216.

    3D structure databases

    ProteinModelPortali Q99618.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 123657. 10 interactions.
    IntActi Q99618. 4 interactions.
    MINTi MINT-1441851.
    STRINGi 9606.ENSP00000229265.

    PTM databases

    PhosphoSitei Q99618.

    Polymorphism databases

    DMDMi 74732787.

    Proteomic databases

    MaxQBi Q99618.
    PaxDbi Q99618.
    PRIDEi Q99618.

    Protocols and materials databases

    DNASUi 83461.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000535406 ; ENSP00000446339 ; ENSG00000111665 .
    ENST00000538862 ; ENSP00000442068 ; ENSG00000111665 .
    GeneIDi 83461.
    KEGGi hsa:83461.
    UCSCi uc001qrg.2. human.

    Organism-specific databases

    CTDi 83461.
    GeneCardsi GC12M006959.
    HGNCi HGNC:14624. CDCA3.
    HPAi HPA026587.
    MIMi 607749. gene.
    neXtProti NX_Q99618.
    PharmGKBi PA26276.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG46427.
    HOGENOMi HOG000013100.
    HOVERGENi HBG053225.
    InParanoidi Q99618.
    OMAi KTGGRAW.
    PhylomeDBi Q99618.
    TreeFami TF101068.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .

    Miscellaneous databases

    GeneWikii CDCA3.
    GenomeRNAii 83461.
    NextBioi 72385.
    PROi Q99618.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q99618.
    Bgeei Q99618.
    CleanExi HS_CDCA3.
    Genevestigatori Q99618.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Drug target discovery by gene expression analysis: cell cycle genes."
      Walker M.G.
      Curr. Cancer Drug Targets 1:73-83(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Large-scale sequencing in human chromosome 12p13: experimental and computational gene structure determination."
      Ansari-Lari M.A., Shen Y., Muzny D.M., Lee W., Gibbs R.A.
      Genome Res. 7:268-280(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: Brain.
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Placenta and Testis.
    6. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
      Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
      Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199 AND SER-209, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    7. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
      Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
      J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199; THR-202 AND SER-209, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    8. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
      Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
      Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199 AND SER-209, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    9. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-29; SER-31; THR-37; SER-44; SER-64; THR-76; SER-87; SER-199 AND SER-209, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    10. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-87 AND SER-199, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.
    12. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-29; SER-94; SER-199 AND SER-209, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    13. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-94, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCDCA3_HUMAN
    AccessioniPrimary (citable) accession number: Q99618
    Secondary accession number(s): A8K5V6, D3DUS6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 15, 2007
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 12
      Human chromosome 12: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways

    External Data

    Dasty 3