Q99618 (CDCA3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cell division cycle-associated protein 3

Alternative name(s):
Gene-rich cluster protein C8
Trigger of mitotic entry protein 1
Short name=TOME-1

Gene names

Name:	CDCA3
Synonyms:	C8, GRCC8, TOME1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	268 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	F-box-like protein which is required for entry into mitosis. Acts by participating in E3 ligase complexes that mediate the ubiquitination and degradation of WEE1 kinase at G2/M phase By similarity.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with SKP1. Part of a SCF (SKP1-cullin-F-box) protein ligase complex By similarity.
Subcellular location	Cytoplasm › cytosol By similarity.
Domain	The KEN box is required for the association with the APC/C-Cdh1 complex By similarity.
Post-translational modification	Ubiquitinated and degraded by the APC/C-Cdh1 complex By similarity.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis Ubl conjugation pathway
Cellular component	Cytoplasm
PTM	Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW protein ubiquitination Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 268	268	Cell division cycle-associated protein 3		PRO_0000287708
Regions
	Region	91 – 120	30	F-box-like
	Motif	258 – 260	3	KEN box
Amino acid modifications
	Modified residue	199	1	Phosphoserine Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11
	Modified residue	202	1	Phosphothreonine Ref.7
	Modified residue	209	1	Phosphoserine Ref.6 Ref.7 Ref.8 Ref.9 Ref.11

Sequences

	Sequence		Length	Mass (Da)
	Q99618 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 48933FA7FF57112C	FASTA	268	28,998
	10 20 30 40 50 60 MGSAKSVPVT PARPPPHNKH LARVADPRSP SAGILRTPIQ VESSPQPGLP AGEQLEGLKH 70 80 90 100 110 120 AQDSDPRSPT LGIARTPMKT SSGDPPSPLV KQLSEVFETE DSKSNLPPEP VLPPEAPLSS 130 140 150 160 170 180 ELDLPLGTQL SVEEQMPPWN QTEFPSKQVF SKEEARQPTE TPVASQSSDK PSRDPETPRS 190 200 210 220 230 240 SGSMRNRWKP NSSKVLGRSP LTILQDDNSP GTLTLRQGKR PSPLSENVSE LKEGAILGTG 250 260 RLLKTGGRAW EQGQDHDKEN QHFPLVES

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Drug target discovery by gene expression analysis: cell cycle genes." Walker M.G. Curr. Cancer Drug Targets 1:73-83(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Large-scale sequencing in human chromosome 12p13: experimental and computational gene structure determination." Ansari-Lari M.A., Shen Y., Muzny D.M., Lee W., Gibbs R.A. Genome Res. 7:268-280(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Brain.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta and Testis.
[6]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199 AND SER-209, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199; THR-202 AND SER-209, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199 AND SER-209, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199 AND SER-209, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[11]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199 AND SER-209, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BG354576 mRNA. No translation available. U47924 Genomic DNA. Translation: AAB51327.1. AK291421 mRNA. Translation: BAF84110.1. CH471116 Genomic DNA. Translation: EAW88728.1. CH471116 Genomic DNA. Translation: EAW88729.1. BC002551 mRNA. Translation: AAH02551.1. BC036512 mRNA. Translation: AAH36512.1.
IPI	IPI00010428.
RefSeq	NP_112589.1. NM_031299.4.
UniGene	Hs.524216.
3D structure databases
ProteinModelPortal	Q99618.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q99618. 4 interactions.
MINT	MINT-1441851.
STRING	9606.ENSP00000229265.
PTM databases
PhosphoSite	Q99618.
Polymorphism databases
DMDM	74732787.
Proteomic databases
PaxDb	Q99618.
PRIDE	Q99618.
Protocols and materials databases
DNASU	83461.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000535406; ENSP00000446339; ENSG00000111665. ENST00000538862; ENSP00000442068; ENSG00000111665. ENST00000598882; ENSP00000469847; ENSG00000268136. ENST00000599860; ENSP00000471183; ENSG00000268136.
GeneID	83461.
KEGG	hsa:83461.
UCSC	uc001qrg.2. human.
Organism-specific databases
CTD	83461.
GeneCards	GC12M006959.
HGNC	HGNC:14624. CDCA3.
HPA	HPA026587.
MIM	607749. gene.
neXtProt	NX_Q99618.
PharmGKB	PA26276.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG46427.
HOGENOM	HOG000013100.
HOVERGEN	HBG053225.
InParanoid	Q99618.
OMA	KTGGRAW.
OrthoDB	EOG4H9XMC.
PhylomeDB	Q99618.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
ArrayExpress	Q99618.
Bgee	Q99618.
CleanEx	HS_CDCA3.
Genevestigator	Q99618.
Family and domain databases
ProtoNet	Search...
Other
GenomeRNAi	83461.
NextBio	72385.
SOURCE	Search...

Entry information

Entry name

CDCA3_HUMAN

Accession

Primary (citable) accession number: Q99618
Secondary accession number(s): A8K5V6, D3DUS6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	May 1, 1997
Last modified:	May 1, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.