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Q99616

- CCL13_HUMAN

UniProt

Q99616 - CCL13_HUMAN

Protein

C-C motif chemokine 13

Gene

CCL13

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. Signals through CCR2B and CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens.

    GO - Molecular functioni

    1. chemokine activity Source: UniProtKB
    2. receptor binding Source: ProtInc

    GO - Biological processi

    1. cell-cell signaling Source: ProtInc
    2. cellular calcium ion homeostasis Source: ProtInc
    3. chemotaxis Source: ProtInc
    4. cytoskeleton organization Source: UniProtKB
    5. eosinophil chemotaxis Source: UniProtKB
    6. immune response Source: InterPro
    7. inflammatory response Source: ProtInc
    8. regulation of cell shape Source: UniProtKB
    9. signal transduction Source: ProtInc

    Keywords - Molecular functioni

    Cytokine

    Keywords - Biological processi

    Chemotaxis, Inflammatory response

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    C-C motif chemokine 13
    Alternative name(s):
    CK-beta-10
    Monocyte chemoattractant protein 4
    Monocyte chemotactic protein 4
    Short name:
    MCP-4
    NCC-1
    Small-inducible cytokine A13
    Cleaved into the following 3 chains:
    Gene namesi
    Name:CCL13
    Synonyms:MCP4, NCC1, SCYA13
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 17

    Organism-specific databases

    HGNCiHGNC:10611. CCL13.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: ProtInc

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA35544.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 16161 PublicationAdd
    BLAST
    Chaini17 – 9882C-C motif chemokine 13, long chainPRO_0000005201Add
    BLAST
    Chaini22 – 9877C-C motif chemokine 13, medium chainPRO_0000005202Add
    BLAST
    Chaini24 – 9875C-C motif chemokine 13, short chainPRO_0000005203Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei24 – 241Pyrrolidone carboxylic acid; in short chain1 Publication
    Disulfide bondi34 ↔ 581 Publication
    Disulfide bondi35 ↔ 741 Publication

    Post-translational modificationi

    One major form (form long), and two minor forms (short chain and medium chain) are produced by differential signal peptide cleavage. The medium chain is about 30-fold less active than the long chain.

    Keywords - PTMi

    Disulfide bond, Pyrrolidone carboxylic acid

    Proteomic databases

    PaxDbiQ99616.
    PRIDEiQ99616.

    PTM databases

    PhosphoSiteiQ99616.

    Miscellaneous databases

    PMAP-CutDBQ99616.

    Expressioni

    Tissue specificityi

    Widely expressed. Found in small intestine, thymus, colon, lung, trachea, stomach and lymph node. Low levels seen in the pulmonary artery smooth muscle cells.

    Inductioni

    By IL1/interleukin-1 and TNF.

    Gene expression databases

    ArrayExpressiQ99616.
    BgeeiQ99616.
    CleanExiHS_CCL13.
    GenevestigatoriQ99616.

    Interactioni

    Protein-protein interaction databases

    BioGridi112260. 2 interactions.
    DIPiDIP-5874N.
    IntActiQ99616. 1 interaction.
    MINTiMINT-103367.
    STRINGi9606.ENSP00000225844.

    Structurei

    Secondary structure

    1
    98
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi32 – 343
    Helixi45 – 473
    Beta strandi48 – 536
    Beta strandi58 – 603
    Beta strandi63 – 675
    Beta strandi72 – 754
    Helixi80 – 8910

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2RA4X-ray1.70A/B24-98[»]
    ProteinModelPortaliQ99616.
    SMRiQ99616. Positions 26-90.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ99616.

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG84946.
    HOGENOMiHOG000036686.
    InParanoidiQ99616.
    KOiK16595.
    OMAiCCFTFSS.
    OrthoDBiEOG7C5MC2.
    PhylomeDBiQ99616.
    TreeFamiTF334888.

    Family and domain databases

    InterProiIPR000827. Chemokine_CC_CS.
    IPR001811. Chemokine_IL8-like_dom.
    [Graphical view]
    PfamiPF00048. IL8. 1 hit.
    [Graphical view]
    SMARTiSM00199. SCY. 1 hit.
    [Graphical view]
    SUPFAMiSSF54117. SSF54117. 1 hit.
    PROSITEiPS00472. SMALL_CYTOKINES_CC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q99616-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKVSAVLLCL LLMTAAFNPQ GLAQPDALNV PSTCCFTFSS KKISLQRLKS   50
    YVITTSRCPQ KAVIFRTKLG KEICADPKEK WVQNYMKHLG RKAHTLKT 98
    Length:98
    Mass (Da):10,986
    Last modified:May 1, 1997 - v1
    Checksum:i612688DFCD308873
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti93 – 986AHTLKT → LTP in CAB01111. 1 PublicationCurated

    Mass spectrometryi

    Molecular mass is 9314±30 Da from positions 17 - 98. Determined by MALDI. 1 Publication
    Molecular mass is 8760±30 Da from positions 22 - 98. Determined by MALDI. 1 Publication
    Molecular mass is 8575±30 Da from positions 24 - 98. Determined by MALDI. 1 Publication

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti5 – 51A → S.
    Corresponds to variant rs3136677 [ dbSNP | Ensembl ].
    VAR_024169
    Natural varianti29 – 291N → S.
    Corresponds to variant rs34566308 [ dbSNP | Ensembl ].
    VAR_048706

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U46767 mRNA. Translation: AAB38703.1.
    X98306 mRNA. Translation: CAA66950.1.
    U59808 mRNA. Translation: AAD09362.1.
    AJ001634 mRNA. Translation: CAA04888.1.
    BT007385 mRNA. Translation: AAP36049.1.
    AC002482 Genomic DNA. Translation: AAB67307.1.
    CR450337 mRNA. Translation: CAG29333.1.
    CH471147 Genomic DNA. Translation: EAW80206.1.
    BC008621 mRNA. Translation: AAH08621.1.
    Z77650 mRNA. Translation: CAB01111.1.
    CCDSiCCDS11281.1.
    RefSeqiNP_005399.1. NM_005408.2.
    UniGeneiHs.414629.

    Genome annotation databases

    EnsembliENST00000225844; ENSP00000225844; ENSG00000181374.
    GeneIDi6357.
    KEGGihsa:6357.
    UCSCiuc002hic.3. human.

    Polymorphism databases

    DMDMi3024128.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Web resourcesi

    Wikipedia

    CCL13 entry

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U46767 mRNA. Translation: AAB38703.1 .
    X98306 mRNA. Translation: CAA66950.1 .
    U59808 mRNA. Translation: AAD09362.1 .
    AJ001634 mRNA. Translation: CAA04888.1 .
    BT007385 mRNA. Translation: AAP36049.1 .
    AC002482 Genomic DNA. Translation: AAB67307.1 .
    CR450337 mRNA. Translation: CAG29333.1 .
    CH471147 Genomic DNA. Translation: EAW80206.1 .
    BC008621 mRNA. Translation: AAH08621.1 .
    Z77650 mRNA. Translation: CAB01111.1 .
    CCDSi CCDS11281.1.
    RefSeqi NP_005399.1. NM_005408.2.
    UniGenei Hs.414629.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2RA4 X-ray 1.70 A/B 24-98 [» ]
    ProteinModelPortali Q99616.
    SMRi Q99616. Positions 26-90.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 112260. 2 interactions.
    DIPi DIP-5874N.
    IntActi Q99616. 1 interaction.
    MINTi MINT-103367.
    STRINGi 9606.ENSP00000225844.

    PTM databases

    PhosphoSitei Q99616.

    Polymorphism databases

    DMDMi 3024128.

    Proteomic databases

    PaxDbi Q99616.
    PRIDEi Q99616.

    Protocols and materials databases

    DNASUi 6357.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000225844 ; ENSP00000225844 ; ENSG00000181374 .
    GeneIDi 6357.
    KEGGi hsa:6357.
    UCSCi uc002hic.3. human.

    Organism-specific databases

    CTDi 6357.
    GeneCardsi GC17P032684.
    HGNCi HGNC:10611. CCL13.
    MIMi 601391. gene.
    neXtProti NX_Q99616.
    PharmGKBi PA35544.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG84946.
    HOGENOMi HOG000036686.
    InParanoidi Q99616.
    KOi K16595.
    OMAi CCFTFSS.
    OrthoDBi EOG7C5MC2.
    PhylomeDBi Q99616.
    TreeFami TF334888.

    Miscellaneous databases

    EvolutionaryTracei Q99616.
    GenomeRNAii 6357.
    NextBioi 24692.
    PMAP-CutDB Q99616.
    PROi Q99616.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q99616.
    Bgeei Q99616.
    CleanExi HS_CCL13.
    Genevestigatori Q99616.

    Family and domain databases

    InterProi IPR000827. Chemokine_CC_CS.
    IPR001811. Chemokine_IL8-like_dom.
    [Graphical view ]
    Pfami PF00048. IL8. 1 hit.
    [Graphical view ]
    SMARTi SM00199. SCY. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54117. SSF54117. 1 hit.
    PROSITEi PS00472. SMALL_CYTOKINES_CC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of MCP-4, a novel CC chemokine related to MCP-1 and eotaxin."
      Power C.A., Meyer A., Rison S.C.G., Guye-Coulin F., Wells T.N.C.
      Eur. Cytokine Netw. 7:513-513(1996)
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Lung.
    2. "Human monocyte chemoattractant protein (MCP)-4 is a novel CC chemokine with activities on monocytes, eosinophils, and basophils induced in allergic and nonallergic inflammation that signals through the CC chemokine receptors (CCR)-2 and -3."
      Garcia-Zepeda E.A., Combadiere C., Rothenberg M.E., Sarafi M.N., Lavigne F., Hamid Q., Murphy P.M., Luster A.D.
      J. Immunol. 157:5613-5626(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Heart.
    3. "Monocyte chemotactic protein 4 (MCP-4), a novel structural and functional analogue of MCP-3 and eotaxin."
      Uguccioni M., Loetscher P., Forssmann U., Dewald B., Li H., Lima S.H., Li Y., Kreider B., Garotta G., Thelen M., Baggiolini M.
      J. Exp. Med. 183:2379-2384(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 17-98, PYROGLUTAMATE FORMATION AT GLN-24, MASS SPECTROMETRY.
      Tissue: Fetus.
    4. "Cloning, in vitro expression, and functional characterization of a novel human CC chemokine of the monocyte chemotactic protein (MCP) family (MCP-4) that binds and signals through the CC chemokine receptor 2B."
      Berkhout T.A., Sarau H.M., Moores K., White J.R., Elshourbagy N., Appelbaum E., Reape T.J., Brawner M., Makwana J., Foley J.J., Schmidt D.B., Imburgia C., Macnulty D., Matthews J., O'Donnell K., O'Shannessy D., Scott M., Groot P.H.E., Macphee C.
      J. Biol. Chem. 272:16404-16413(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 22-33, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Fetus.
    5. "Monocyte chemotactic protein-4: tissue-specific expression and signaling through CC chemokine receptor-2."
      Godiska R., Chantry D., Raport C.J., Schweickart V.L., Trong H.L., Gray P.W.
      J. Leukoc. Biol. 61:353-360(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    6. "Genomic organization, sequence analysis and transcriptional regulation of the human MCP-4 chemokine gene (SCYA13) in dermal fibroblasts: a comparison to other eosinophilic beta-chemokines."
      Hein H., Schluter C., Kulke R., Christophers E., Schroeder J.-M., Bartels J.
      Biochem. Biophys. Res. Commun. 255:470-476(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Skin fibroblast.
    7. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    8. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
      Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    9. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
      Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
      , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
      Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    10. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    11. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    12. "Expression of a MCP-4 like novel CC-chemokine in human dermal fibroblasts: molecular cloning and RT-PCR analysis."
      Bartels J.H., Schlueter C., Richter E., Noso N., Christophers E., Schroeder J.-M.
      Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 17-95.
      Tissue: Foreskin.
    13. "Structure of human monocyte chemoattractant protein 4 (MCP-4/CCL13)."
      Barinka C., Prahl A., Lubkowski J.
      Acta Crystallogr. D 64:273-278(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 24-98, DISULFIDE BONDS.

    Entry informationi

    Entry nameiCCL13_HUMAN
    AccessioniPrimary (citable) accession number: Q99616
    Secondary accession number(s): O95689, Q6ICQ6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 142 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    This protein can bind heparin.

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 17
      Human chromosome 17: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3