Q99616 (CCL13_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
C-C motif chemokine 13

Alternative name(s):
CK-beta-10
Monocyte chemoattractant protein 4
Monocyte chemotactic protein 4
Short name=MCP-4
NCC-1
Small-inducible cytokine A13

Cleaved into the following 3 chains:

1. C-C motif chemokine 13, long chain
2. C-C motif chemokine 13, medium chain
3. C-C motif chemokine 13, short chain

Gene names

Name:	CCL13
Synonyms:	MCP4, NCC1, SCYA13

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	98 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. Signals through CCR2B and CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens.
Subcellular location	Secreted.
Tissue specificity	Widely expressed. Found in small intestine, thymus, colon, lung, trachea, stomach and lymph node. Low levels seen in the pulmonary artery smooth muscle cells.
Induction	By IL1/interleukin-1 and TNF.
Post-translational modification	One major form (form long), and two minor forms (short chain and medium chain) are produced by differential signal peptide cleavage. The medium chain is about 30-fold less active than the long chain.
Miscellaneous	This protein can bind heparin.
Sequence similarities	Belongs to the intercrine beta (chemokine CC) family.
Mass spectrometry	Molecular mass is 9314±30 Da from positions 17 - 98. Determined by MALDI. Ref.3 Molecular mass is 8760±30 Da from positions 22 - 98. Determined by MALDI. Ref.3 Molecular mass is 8575±30 Da from positions 24 - 98. Determined by MALDI. Ref.3

Ontologies

Keywords
Biological process	Chemotaxis Inflammatory response
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Cytokine
PTM	Disulfide bond Pyrrolidone carboxylic acid
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cell-cell signaling Traceable author statement Ref.4. Source: ProtInc cellular calcium ion homeostasis Traceable author statement Ref.4. Source: ProtInc cytoskeleton organization Inferred from direct assay PubMed 10072545. Source: UniProtKB eosinophil chemotaxis Inferred from direct assay PubMed 10072545. Source: UniProtKB immune response Inferred from electronic annotation. Source: InterPro inflammatory response Traceable author statement Ref.4. Source: ProtInc regulation of cell shape Inferred from direct assay PubMed 10072545. Source: UniProtKB signal transduction Traceable author statement Ref.4PubMed 9558100. Source: ProtInc
Cellular_component	extracellular space Traceable author statement Ref.4. Source: ProtInc
Molecular_function	chemokine activity Inferred from direct assay PubMed 10072545. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 16

16

Ref.3

Chain

17 – 98

82

C-C motif chemokine 13, long chain

PRO_0000005201

Chain

22 – 98

77

C-C motif chemokine 13, medium chain

PRO_0000005202

Chain

24 – 98

75

C-C motif chemokine 13, short chain

PRO_0000005203

Amino acid modifications

Modified residue

24

1

Pyrrolidone carboxylic acid; in short chain

Disulfide bond

34 ↔ 58

Ref.13

Disulfide bond

35 ↔ 74

Ref.13

Natural variations

Natural variant

5

1

A → S.
Corresponds to variant rs3136677 [ dbSNP | Ensembl ].

VAR_024169

Natural variant

29

1

N → S.
Corresponds to variant rs34566308 [ dbSNP | Ensembl ].

VAR_048706

Experimental info

Sequence conflict

93 – 98

6

AHTLKT → LTP in CAB01111. Ref.12

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

98

Helix Strand Turn

Details...

Beta strand

32 – 34

3

Helix

45 – 47

3

Beta strand

48 – 53

6

Beta strand

58 – 60

3

Beta strand

63 – 67

5

Beta strand

72 – 75

4

Helix

80 – 89

10

Sequences

	Sequence		Length	Mass (Da)
	Q99616 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 612688DFCD308873	FASTA	98	10,986
	10 20 30 40 50 60 MKVSAVLLCL LLMTAAFNPQ GLAQPDALNV PSTCCFTFSS KKISLQRLKS YVITTSRCPQ 70 80 90 KAVIFRTKLG KEICADPKEK WVQNYMKHLG RKAHTLKT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of MCP-4, a novel CC chemokine related to MCP-1 and eotaxin." Power C.A., Meyer A., Rison S.C.G., Guye-Coulin F., Wells T.N.C. Eur. Cytokine Netw. 7:513-513(1996) Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lung.
[2]	"Human monocyte chemoattractant protein (MCP)-4 is a novel CC chemokine with activities on monocytes, eosinophils, and basophils induced in allergic and nonallergic inflammation that signals through the CC chemokine receptors (CCR)-2 and -3." Garcia-Zepeda E.A., Combadiere C., Rothenberg M.E., Sarafi M.N., Lavigne F., Hamid Q., Murphy P.M., Luster A.D. J. Immunol. 157:5613-5626(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Heart.
[3]	"Monocyte chemotactic protein 4 (MCP-4), a novel structural and functional analogue of MCP-3 and eotaxin." Uguccioni M., Loetscher P., Forssmann U., Dewald B., Li H., Lima S.H., Li Y., Kreider B., Garotta G., Thelen M., Baggiolini M. J. Exp. Med. 183:2379-2384(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 17-98, MASS SPECTROMETRY. Tissue: Fetus.
[4]	"Cloning, in vitro expression, and functional characterization of a novel human CC chemokine of the monocyte chemotactic protein (MCP) family (MCP-4) that binds and signals through the CC chemokine receptor 2B." Berkhout T.A., Sarau H.M., Moores K., White J.R., Elshourbagy N., Appelbaum E., Reape T.J., Brawner M., Makwana J., Foley J.J., Schmidt D.B., Imburgia C., Macnulty D., Matthews J., O'Donnell K., O'Shannessy D., Scott M., Groot P.H.E., Macphee C. J. Biol. Chem. 272:16404-16413(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 22-33, MASS SPECTROMETRY. Tissue: Fetus.
[5]	"Monocyte chemotactic protein-4: tissue-specific expression and signaling through CC chemokine receptor-2." Godiska R., Chantry D., Raport C.J., Schweickart V.L., Trong H.L., Gray P.W. J. Leukoc. Biol. 61:353-360(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[6]	"Genomic organization, sequence analysis and transcriptional regulation of the human MCP-4 chemokine gene (SCYA13) in dermal fibroblasts: a comparison to other eosinophilic beta-chemokines." Hein H., Schluter C., Kulke R., Christophers E., Schroeder J.-M., Bartels J. Biochem. Biophys. Res. Commun. 255:470-476(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Skin fibroblast.
[7]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[8]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[9]	"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C. Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[10]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[11]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[12]	"Expression of a MCP-4 like novel CC-chemokine in human dermal fibroblasts: molecular cloning and RT-PCR analysis." Bartels J.H., Schlueter C., Richter E., Noso N., Christophers E., Schroeder J.-M. Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 17-95. Tissue: Foreskin.
[13]	"Structure of human monocyte chemoattractant protein 4 (MCP-4/CCL13)." Barinka C., Prahl A., Lubkowski J. Acta Crystallogr. D 64:273-278(2008) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 24-98, DISULFIDE BONDS.
+	Additional computationally mapped references.

Web resources

Wikipedia

CCL13 entry

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U46767 mRNA. Translation: AAB38703.1.
X98306 mRNA. Translation: CAA66950.1.
U59808 mRNA. Translation: AAD09362.1.
AJ001634 mRNA. Translation: CAA04888.1.
BT007385 mRNA. Translation: AAP36049.1.
AC002482 Genomic DNA. Translation: AAB67307.1.
CR450337 mRNA. Translation: CAG29333.1.
CH471147 Genomic DNA. Translation: EAW80206.1.
BC008621 mRNA. Translation: AAH08621.1.
Z77650 mRNA. Translation: CAB01111.1.

IPI

IPI00016914.

RefSeq

NP_005399.1. NM_005408.2.

UniGene

Hs.414629.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2RA4	X-ray	1.70	A/B	24-98	[»]

ProteinModelPortal

Q99616.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-5874N.

IntAct

Q99616. 1 interaction.

MINT

MINT-103367.

STRING

9606.ENSP00000225844.

PTM databases

PhosphoSite

Q99616.

Polymorphism databases

DMDM

3024128.

Proteomic databases

PaxDb

Q99616.

PRIDE

Q99616.

Protocols and materials databases

DNASU

6357.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000225844; ENSP00000225844; ENSG00000181374.

GeneID

6357.

KEGG

hsa:6357.

UCSC

uc002hic.3. human.

Organism-specific databases

CTD

6357.

GeneCards

GC17P032684.

HGNC

HGNC:10611. CCL13.

MIM

601391. gene.

neXtProt

NX_Q99616.

PharmGKB

PA35544.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG84946.

HOGENOM

HOG000036686.

InParanoid

Q99616.

KO

K16595.

OMA

SKKISLQ.

OrthoDB

EOG45DWR1.

PhylomeDB

Q99616.

Gene expression databases

ArrayExpress

Q99616.

Bgee

Q99616.

CleanEx

HS_CCL13.

Genevestigator

Q99616.

GermOnline

ENSG00000181374. Homo sapiens.

Family and domain databases

InterPro

IPR000827. Chemokine_CC_CS.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]

Pfam

PF00048. IL8. 1 hit.
[Graphical view]

SMART

SM00199. SCY. 1 hit.
[Graphical view]

SUPFAM

SSF54117. Chemokine_IL8. 1 hit.

PROSITE

PS00472. SMALL_CYTOKINES_CC. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q99616.

GenomeRNAi

6357.

NextBio

24692.

PMAP-CutDB

Q99616.

SOURCE

Search...

Entry information

Entry name

CCL13_HUMAN

Accession

Primary (citable) accession number: Q99616
Secondary accession number(s): O95689, Q6ICQ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	May 1, 1997
Last modified:	May 1, 2013
This is version 132 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.