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Q99616 (CCL13_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 139. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
C-C motif chemokine 13
Alternative name(s):
CK-beta-10
Monocyte chemoattractant protein 4
Monocyte chemotactic protein 4
Short name=MCP-4
NCC-1
Small-inducible cytokine A13
Gene names
Name:CCL13
Synonyms:MCP4, NCC1, SCYA13
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length98 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. Signals through CCR2B and CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens.

Subcellular location

Secreted.

Tissue specificity

Widely expressed. Found in small intestine, thymus, colon, lung, trachea, stomach and lymph node. Low levels seen in the pulmonary artery smooth muscle cells.

Induction

By IL1/interleukin-1 and TNF.

Post-translational modification

One major form (form long), and two minor forms (short chain and medium chain) are produced by differential signal peptide cleavage. The medium chain is about 30-fold less active than the long chain.

Miscellaneous

This protein can bind heparin.

Sequence similarities

Belongs to the intercrine beta (chemokine CC) family.

Mass spectrometry

Molecular mass is 9314±30 Da from positions 17 - 98. Determined by MALDI. Ref.3

Molecular mass is 8760±30 Da from positions 22 - 98. Determined by MALDI. Ref.3

Molecular mass is 8575±30 Da from positions 24 - 98. Determined by MALDI. Ref.3

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1616 Ref.3
Chain17 – 9882C-C motif chemokine 13, long chain
PRO_0000005201
Chain22 – 9877C-C motif chemokine 13, medium chain
PRO_0000005202
Chain24 – 9875C-C motif chemokine 13, short chain
PRO_0000005203

Amino acid modifications

Modified residue241Pyrrolidone carboxylic acid; in short chain
Disulfide bond34 ↔ 58 Ref.13
Disulfide bond35 ↔ 74 Ref.13

Natural variations

Natural variant51A → S.
Corresponds to variant rs3136677 [ dbSNP | Ensembl ].
VAR_024169
Natural variant291N → S.
Corresponds to variant rs34566308 [ dbSNP | Ensembl ].
VAR_048706

Experimental info

Sequence conflict93 – 986AHTLKT → LTP in CAB01111. Ref.12

Secondary structure

.............. 98
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q99616 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 612688DFCD308873

FASTA9810,986
        10         20         30         40         50         60 
MKVSAVLLCL LLMTAAFNPQ GLAQPDALNV PSTCCFTFSS KKISLQRLKS YVITTSRCPQ 

        70         80         90 
KAVIFRTKLG KEICADPKEK WVQNYMKHLG RKAHTLKT 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of MCP-4, a novel CC chemokine related to MCP-1 and eotaxin."
Power C.A., Meyer A., Rison S.C.G., Guye-Coulin F., Wells T.N.C.
Eur. Cytokine Netw. 7:513-513(1996)
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Lung.
[2]"Human monocyte chemoattractant protein (MCP)-4 is a novel CC chemokine with activities on monocytes, eosinophils, and basophils induced in allergic and nonallergic inflammation that signals through the CC chemokine receptors (CCR)-2 and -3."
Garcia-Zepeda E.A., Combadiere C., Rothenberg M.E., Sarafi M.N., Lavigne F., Hamid Q., Murphy P.M., Luster A.D.
J. Immunol. 157:5613-5626(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Heart.
[3]"Monocyte chemotactic protein 4 (MCP-4), a novel structural and functional analogue of MCP-3 and eotaxin."
Uguccioni M., Loetscher P., Forssmann U., Dewald B., Li H., Lima S.H., Li Y., Kreider B., Garotta G., Thelen M., Baggiolini M.
J. Exp. Med. 183:2379-2384(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 17-98, MASS SPECTROMETRY.
Tissue: Fetus.
[4]"Cloning, in vitro expression, and functional characterization of a novel human CC chemokine of the monocyte chemotactic protein (MCP) family (MCP-4) that binds and signals through the CC chemokine receptor 2B."
Berkhout T.A., Sarau H.M., Moores K., White J.R., Elshourbagy N., Appelbaum E., Reape T.J., Brawner M., Makwana J., Foley J.J., Schmidt D.B., Imburgia C., Macnulty D., Matthews J., O'Donnell K., O'Shannessy D., Scott M., Groot P.H.E., Macphee C.
J. Biol. Chem. 272:16404-16413(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 22-33, IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: Fetus.
[5]"Monocyte chemotactic protein-4: tissue-specific expression and signaling through CC chemokine receptor-2."
Godiska R., Chantry D., Raport C.J., Schweickart V.L., Trong H.L., Gray P.W.
J. Leukoc. Biol. 61:353-360(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[6]"Genomic organization, sequence analysis and transcriptional regulation of the human MCP-4 chemokine gene (SCYA13) in dermal fibroblasts: a comparison to other eosinophilic beta-chemokines."
Hein H., Schluter C., Kulke R., Christophers E., Schroeder J.-M., Bartels J.
Biochem. Biophys. Res. Commun. 255:470-476(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Skin fibroblast.
[7]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[8]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[9]"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. expand/collapse author list , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[10]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[11]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[12]"Expression of a MCP-4 like novel CC-chemokine in human dermal fibroblasts: molecular cloning and RT-PCR analysis."
Bartels J.H., Schlueter C., Richter E., Noso N., Christophers E., Schroeder J.-M.
Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 17-95.
Tissue: Foreskin.
[13]"Structure of human monocyte chemoattractant protein 4 (MCP-4/CCL13)."
Barinka C., Prahl A., Lubkowski J.
Acta Crystallogr. D 64:273-278(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 24-98, DISULFIDE BONDS.
+Additional computationally mapped references.

Web resources

Wikipedia

CCL13 entry

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U46767 mRNA. Translation: AAB38703.1.
X98306 mRNA. Translation: CAA66950.1.
U59808 mRNA. Translation: AAD09362.1.
AJ001634 mRNA. Translation: CAA04888.1.
BT007385 mRNA. Translation: AAP36049.1.
AC002482 Genomic DNA. Translation: AAB67307.1.
CR450337 mRNA. Translation: CAG29333.1.
CH471147 Genomic DNA. Translation: EAW80206.1.
BC008621 mRNA. Translation: AAH08621.1.
Z77650 mRNA. Translation: CAB01111.1.
RefSeqNP_005399.1. NM_005408.2.
UniGeneHs.414629.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2RA4X-ray1.70A/B24-98[»]
ProteinModelPortalQ99616.
SMRQ99616. Positions 26-90.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112260. 2 interactions.
DIPDIP-5874N.
IntActQ99616. 1 interaction.
MINTMINT-103367.
STRING9606.ENSP00000225844.

PTM databases

PhosphoSiteQ99616.

Polymorphism databases

DMDM3024128.

Proteomic databases

PaxDbQ99616.
PRIDEQ99616.

Protocols and materials databases

DNASU6357.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000225844; ENSP00000225844; ENSG00000181374.
GeneID6357.
KEGGhsa:6357.
UCSCuc002hic.3. human.

Organism-specific databases

CTD6357.
GeneCardsGC17P032684.
HGNCHGNC:10611. CCL13.
MIM601391. gene.
neXtProtNX_Q99616.
PharmGKBPA35544.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG84946.
HOGENOMHOG000036686.
InParanoidQ99616.
KOK16595.
OMACCFTFSS.
OrthoDBEOG7C5MC2.
PhylomeDBQ99616.
TreeFamTF334888.

Gene expression databases

ArrayExpressQ99616.
BgeeQ99616.
CleanExHS_CCL13.
GenevestigatorQ99616.

Family and domain databases

InterProIPR000827. Chemokine_CC_CS.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]
PfamPF00048. IL8. 1 hit.
[Graphical view]
SMARTSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMSSF54117. SSF54117. 1 hit.
PROSITEPS00472. SMALL_CYTOKINES_CC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ99616.
GenomeRNAi6357.
NextBio24692.
PMAP-CutDBQ99616.
PROQ99616.
SOURCESearch...

Entry information

Entry nameCCL13_HUMAN
AccessionPrimary (citable) accession number: Q99616
Secondary accession number(s): O95689, Q6ICQ6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: May 1, 1997
Last modified: April 16, 2014
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM