Q99611 (SPS2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Selenide, water dikinase 2
EC=2.7.9.3

Alternative name(s):
Selenium donor protein 2
Selenophosphate synthase 2

Gene names

Name:	SEPHS2
Synonyms:	SPS2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	448 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Synthesizes selenophosphate from selenide and ATP.
Catalytic activity	ATP + selenide + H2O = AMP + selenophosphate + phosphate.
Sequence similarities	Belongs to the selenophosphate synthase 1 family. Class I subfamily.

Ontologies

Keywords
Coding sequence diversity	Polymorphism Selenocysteine
Ligand	ATP-binding Nucleotide-binding Selenium
Molecular function	Kinase Transferase
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	selenocysteine biosynthetic process Non-traceable author statement Ref.2. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW selenide, water dikinase activity Non-traceable author statement Ref.2. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 448	447	Selenide, water dikinase 2		PRO_0000127650
Regions
	Nucleotide binding	319 – 325	7	ATP Potential
Sites
	Active site	60	1	Potential
	Site	63	1	Important for catalytic activity By similarity
Amino acid modifications
	Non-standard residue	60	1	Selenocysteine
	Modified residue	2	1	N-acetylalanine Ref.5
Natural variations
	Natural variant	269	1	P → A. Corresponds to variant rs1804600 [ dbSNP | Ensembl ].		VAR_052345

Sequences

	Sequence		Length	Mass (Da)
	Q99611 [UniParc]. Last modified February 26, 2008. Version 3. Checksum: 309520C0CAE770F7	FASTA	448	47,305
	10 20 30 40 50 60 MAEASATGAC GEAMAAAEGS SGPAGLTLGR SFSNYRPFEP QALGLSPSWR LTGFSGMKGU 70 80 90 100 110 120 GCKVPQEALL KLLAGLTRPD VRPPLGRGLV GGQEEASQEA GLPAGAGPSP TFPALGIGMD 130 140 150 160 170 180 SCVIPLRHGG LSLVQTTDFF YPLVEDPYMM GRIACANVLS DLYAMGITEC DNMLMLLSVS 190 200 210 220 230 240 QSMSEEEREK VTPLMVKGFR DAAEEGGTAV TGGQTVVNPW IIIGGVATVV CQPNEFIMPD 250 260 270 280 290 300 SAVVGDVLVL TKPLGTQVAV NAHQWLDNPE RWNKVKMVVS REEVELAYQE AMFNMATLNR 310 320 330 340 350 360 TAAGLMHTFN AHAATDITGF GILGHSQNLA KQQRNEVSFV IHNLPIIAKM AAVSKASGRF 370 380 390 400 410 420 GLLQGTSAET SGGLLICLPR EQAARFCSEI KSSKYGEGHQ AWIVGIVEKG NRTARIIDKP 430 440 RVIEVLPRGA TAAVLAPDSS NASSEPSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A new approach to the study of haematopoietic development in the yolk sac and embryoid bodies." Guimaraes M.J., Bazan J.F., Zlotnik A., Wiles M.V., Grimaldi J.C., Lee F., McClanahan T. Development 121:3335-3346(1995) [PubMed: 7588067] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Identification of a novel selD homolog from eukaryotes, bacteria, and archaea: is there an autoregulatory mechanism in selenocysteine metabolism?" Guimaraes M.J., Peterson D., Vicari A., Cocks B.G., Copeland N.G., Gilbert D.J., Jenkins N.A., Ferrick D.A., Kastelein R., Bazan J.F., Zlotnik A. Proc. Natl. Acad. Sci. U.S.A. 93:15086-15091(1996) [PubMed: 8986768] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The sequence and analysis of duplication-rich human chromosome 16." Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J. , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A. Nature 432:988-994(2004) [PubMed: 15616553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Muscle and Skin.
[5]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, MASS SPECTROMETRY. Tissue: Embryonic kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U43286 mRNA. Translation: AAC50958.2. AC116348 Genomic DNA. No translation available. BC002381 mRNA. Translation: AAH02381.3. BC016643 mRNA. Translation: AAH16643.1.
IPI	IPI00016906.
RefSeq	NP_036380.2. NM_012248.2.
UniGene	Hs.118725.
3D structure databases
ProteinModelPortal	Q99611.
SMR	Q99611. Positions 38-427.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q99611. 1 interaction.
STRING	Q99611.
PTM databases
PhosphoSite	Q99611.
Polymorphism databases
DMDM	172044671.
Proteomic databases
PRIDE	Q99611.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000478753; ENSP00000418669; ENSG00000179918.
GeneID	22928.
KEGG	hsa:22928.
Organism-specific databases
CTD	22928.
GeneCards	GC16M030454.
H-InvDB	HIX0012959.
HGNC	HGNC:19686. SEPHS2.
MIM	606218. gene.
neXtProt	NX_Q99611.
GenAtlas	Search...
Phylogenomic databases
GeneTree	ENSGT00390000000950.
HOGENOM	HBG383791.
HOVERGEN	HBG001207.
InParanoid	Q99611.
OMA	GMDSCVI.
OrthoDB	EOG4ZW5B4.
PhylomeDB	Q99611.
Enzyme and pathway databases
BRENDA	2.7.9.3. 2681.
Gene expression databases
ArrayExpress	Q99611.
Bgee	Q99611.
CleanEx	HS_SEPHS2.
Genevestigator	Q99611.
Family and domain databases
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR016188. PurM_N-like. IPR004536. SelD. [Graphical view]
KO	K01008.
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 1 hit. SSF55326. PurM_N-like. 1 hit.
TIGRFAMs	TIGR00476. SelD. 1 hit.
ProtoNet	Search...
Other
NextBio	43647.
SOURCE	Search...

Entry information

Entry name

SPS2_HUMAN

Accession

Primary (citable) accession number: Q99611
Secondary accession number(s): Q9BUQ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	February 26, 2008
Last modified:	January 25, 2012
This is version 100 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.