Reviewed, UniProtKB/Swiss-Prot Q99608 (NECD_HUMAN)

Names and origin

Protein names

Recommended name:
    Necdin

Gene names

Name:

NDN

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Growth suppressor that facilitates the entry of the cell into cell cycle arrest. Functionally similar to the retinoblastoma protein it binds to and represses the activity of cell-cycle-promoting proteins such as SV40 large T antigen, adenovirus E1A, and the transcription factor E2F. Necdin also interacts with p53 and works in an additive manner to inhibit cell growth. Functions also as transcription factor and binds directly to specific guanosine-rich DNA sequences By similarity.
Subunit structure	Binds to the transactivation domains of E2F1 and p53. Binds also SV40 large T antigen and adenovirus E1A. Interacts with nucleobindin 1 and 2 By similarity.
Subcellular location	Perikaryon. Nucleus. Note: Neural perikarya, translocates to the nucleus of postmitotic neurons and interacts with the nuclear matrix.
Tissue specificity	Almost ubiquitous. Detected in fetal brain, lung, liver and kidney; in adult heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine and colon. Not detected in peripheral blood leukocytes. In brain, restricted to post-mitotic neurons.
Involvement in disease	Defects in NDN may be a cause of the Prader-Willi syndrome (PWS) which is a contiguous gene syndrome resulting from inactivity of the paternal copies of a number of genes on 15q11, through deletion or disruption of these genes or maternal uniparental disomy 15. The PWS syndrome is characterized by muscular hypotonia, mental retardation, short stature, obesity, hypogonadotropic hypogonadism, and small hands and feet.
Sequence similarities	Contains 1 MAGE domain.

Ontologies

Keywords
Biological process	Growth regulation Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	negative regulation of cell proliferation Ref.2 Traceable author statement. Source: ProtInc regulation of growth Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell perikaryon Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
AUP1	Q9Y679	1	EBI-718177,EBI-1058701
DHCR7	Q9UBM7	1	EBI-718177,EBI-1054468
LASS2	Q96G23	1	EBI-718177,EBI-1057080
RPS11	P62280	1	EBI-718177,EBI-1047710
SEC61A1	P61619	1	EBI-718177,EBI-358919
SRPRB	Q9Y5M8	1	EBI-718177,EBI-355393
TMEM33	P57088	1	EBI-718177,EBI-1048629

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 321	321	Necdin		PRO_0000156740
Regions
	Domain	98 – 297	200	MAGE

Sequences

	Sequence		Length	Mass (Da)
	Q99608-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 5068FE121308BFAF	FASTA	321	36,086
	10 20 30 40 50 60 MSEQSKDLSD PNFAAEAPNS EVHSSPGVSE GVPPSATLAE PQSPPLGPTA APQAAPPPQA 70 80 90 100 110 120 PNDEGDPKAL QQAAEEGRAH QAPSAAQPGP APPAPAQLVQ KAHELMWYVL VKDQKKMIIW 130 140 150 160 170 180 FPDMVKDVIG SYKKWCRSIL RRTSLILARV FGLHLRLTSL HTMEFALVKA LEPEELDRVA 190 200 210 220 230 240 LSNRMPMTGL LLMILSLIYV KGRGARESAV WNVLRILGLR PWKKHSTFGD VRKLITEEFV 250 260 270 280 290 300 QMNYLKYQRV PYVEPPEYEF FWGSRASREI TKMQIMEFLA RVFKKDPQAW PSRYREALEE 310 320 ARALREANPT AHYPRSSVSE D

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The human necdin gene, NDN, is maternally imprinted and located in the Prader-Willi syndrome chromosomal region." Jay P., Rougeulle C., Massacrier A., Moncla A., Mattei M.-G., Malzac P., Roeckel N., Taviaux S., Lefranc J.-L.B., Cau P., Berta P., Lalande M., Muscatelli F. Nat. Genet. 17:357-360(1997) [PubMed: 9354807] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Embryo.
[2]	"The human chromosomal gene for necdin, a neuronal growth suppressor, in the Prader-Willi syndrome deletion region." Nakada Y., Taniura H., Uetsuki T., Inazawa J., Yoshikawa K. Gene 213:65-72(1998) [PubMed: 9630521] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U35139 mRNA. Translation: AAB39469.1. AB007828 Genomic DNA. Translation: BAA22660.1. AK312779 mRNA. Translation: BAG35642.1. BC008750 mRNA. Translation: AAH08750.1.
IPI	IPI00001102.
RefSeq	NP_002478.1.
UniGene	Hs.50130
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q99608. 9 interactions.
STRING	Q99608.
Proteomic databases
PRIDE	Q99608.
Genome annotation databases
Ensembl	ENST00000331837; ENSP00000332643; ENSG00000182636; Homo sapiens. [Genome view]
GeneID	4692.
KEGG	hsa:4692.
UCSC	uc001ywk.1. human.
Organism-specific databases
CTD	4692.
GeneCards	GC15M021481.
H-InvDB	HIX0012046.
HGNC	HGNC:7675. NDN.
MIM	176270. phenotype. 602117. gene.
Orphanet	739. Prader-Willi syndrome.
PharmGKB	PA31479.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG05927.
HOGENOM	HBG126941.
HOVERGEN	Q99608.
InParanoid	Q99608.
OMA	KRMIIWF.
OrthoDB	EOG9VX4RC.
PhylomeDB	Q99608.
Enzyme and pathway databases
Pathway_Interaction_DB	p75ntrpathway. p75(NTR)-mediated signaling.
Gene expression databases
ArrayExpress	Q99608.
Bgee	Q99608.
CleanEx	HS_NDN.
Genevestigator	Q99608.
GermOnline	ENSG00000182636. Homo sapiens.
Family and domain databases
InterPro	IPR002190. MAGE. [Graphical view]
PANTHER	PTHR11736. MAGE. 1 hit.
Pfam	PF01454. MAGE. 1 hit. [Graphical view]
PROSITE	PS50838. MAGE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	18094.
SOURCE	Search...

Entry information

Entry name

NECD_HUMAN

Accession

Primary (citable) accession number: Q99608
Secondary accession number(s): B2R6Z5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 18, 2001
Last sequence update:	May 1, 1997
Last modified:	January 19, 2010
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.