Q99594 (TEAD3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcriptional enhancer factor TEF-5

Alternative name(s):
DTEF-1
TEA domain family member 3
Short name=TEAD-3

Gene names

Name:	TEAD3
Synonyms:	TEAD5, TEF5

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	435 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds to multiple functional elements of the human chorionic somatomammotropin-B gene enhancer. Ref.4 Ref.5
Subunit structure	Interacts with YAP1 and WWTR1/TAZ. Ref.4 Ref.5
Subcellular location	Nucleus.
Tissue specificity	Preferentially expressed in the placenta.
Sequence similarities	Contains 1 TEA DNA-binding domain.
Sequence caution	The sequence AAH27877.1 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical ATA isoleucine codon. The sequence AAH91488.2 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical ATA isoleucine codon. The sequence CAA64213.2 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical ATA isoleucine codon.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Ligand	DNA-binding
Molecular function	Activator
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	female pregnancy Traceable author statement Ref.1. Source: ProtInc hippo signaling cascade Inferred from direct assay Ref.4Ref.5. Source: UniProtKB positive regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara regulation of transcription from RNA polymerase II promoter Traceable author statement Ref.1. Source: ProtInc transcription initiation from RNA polymerase II promoter Traceable author statement. Source: Reactome
Cellular_component	nucleoplasm Traceable author statement. Source: Reactome transcription factor complex Inferred from electronic annotation. Source: Compara
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Inferred from direct assay Ref.4Ref.5. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.7
	Chain	2 – 435	434	Transcriptional enhancer factor TEF-5		PRO_0000205934
Regions
	DNA binding	30 – 97	68	TEA
	Region	173 – 435	263	Transcriptional activation Potential
	Compositional bias	146 – 213	68	Pro-rich
Amino acid modifications
	Modified residue	2	1	N-acetylalanine Ref.7
Natural variations
	Natural variant	254	1	T → M. Corresponds to variant rs35080860 [ dbSNP | Ensembl ].		VAR_052278

Sequences

	Sequence		Length	Mass (Da)
	Q99594 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 9F8E7900EB13D4DC	FASTA	435	48,676
	10 20 30 40 50 60 MASNSWNASS SPGEAREDGP EGLDKGLDND AEGVWSPDIE QSFQEALAIY PPCGRRKIIL 70 80 90 100 110 120 SDEGKMYGRN ELIARYIKLR TGKTRTRKQV SSHIQVLARK KVREYQVGIK AMNLDQVSKD 130 140 150 160 170 180 KALQSMASMS SAQIVSASVL QNKFSPPSPL PQAVFSTSSR FWSSPPLLGQ QPGPSQDIKP 190 200 210 220 230 240 FAQPAYPIQP PLPPTLSSYE PLAPLPSAAA SVPVWQDRTI ASSRLRLLEY SAFMEVQRDP 250 260 270 280 290 300 DTYSKHLFVH IGQTNPAFSD PPLEAVDVRQ IYDKFPEKKG GLKELYEKGP PNAFFLVKFW 310 320 330 340 350 360 ADLNSTIQEG PGAFYGVSSQ YSSADSMTIS VSTKVCSFGK QVVEKVETEY ARLENGRFVY 370 380 390 400 410 420 RIHRSPMCEY MINFIHKLKH LPEKYMMNSV LENFTILQVV TSRDSQETLL VIAFVFEVST 430 SEHGAQHHVY KLVKD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human TEF-5 is preferentially expressed in placenta and binds to multiple functional elements of the human chorionic somatomammotropin-B gene enhancer." Jacquemin P., Martial J.A., Davidson I. J. Biol. Chem. 272:12928-12937(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung and PNS.
[4]	"TEAD mediates YAP-dependent gene induction and growth control." Zhao B., Ye X., Yu J., Li L., Li W., Li S., Yu J., Lin J.D., Wang C.Y., Chinnaiyan A.M., Lai Z.C., Guan K.L. Genes Dev. 22:1962-1971(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH YAP1.
[5]	"TEAD transcription factors mediate the function of TAZ in cell growth and epithelial-mesenchymal transition." Zhang H., Liu C.Y., Zha Z.Y., Zhao B., Yao J., Zhao S., Xiong Y., Lei Q.Y., Guan K.L. J. Biol. Chem. 284:13355-13362(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, INTERACTION WITH WWTR1.
[6]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[7]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, MASS SPECTROMETRY, CLEAVAGE OF INITIATOR METHIONINE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X94439 mRNA. Translation: CAA64213.2. Sequence problems. AL022721 Genomic DNA. No translation available. BC027877 mRNA. Translation: AAH27877.1. Sequence problems. BC091488 mRNA. Translation: AAH91488.2. Sequence problems.
IPI	IPI00412347.
RefSeq	NP_003205.2. NM_003214.3.
UniGene	Hs.485205.
3D structure databases
ProteinModelPortal	Q99594.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q99594. 7 interactions.
STRING	9606.ENSP00000345772.
PTM databases
PhosphoSite	Q99594.
Polymorphism databases
DMDM	2501157.
Proteomic databases
PaxDb	Q99594.
PRIDE	Q99594.
Protocols and materials databases
DNASU	7005.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000338863; ENSP00000345772; ENSG00000007866.
GeneID	7005.
KEGG	hsa:7005.
UCSC	uc003okt.3. human.
Organism-specific databases
CTD	7005.
GeneCards	GC06M035441.
HGNC	HGNC:11716. TEAD3.
HPA	HPA028906.
MIM	603170. gene.
neXtProt	NX_Q99594.
PharmGKB	PA36434.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG246992.
HOGENOM	HOG000253933.
HOVERGEN	HBG056905.
KO	K09448.
OrthoDB	EOG4QNMW5.
Enzyme and pathway databases
Reactome	REACT_71. Gene Expression.
Gene expression databases
ArrayExpress	Q99594.
Bgee	Q99594.
CleanEx	HS_TEAD3.
Genevestigator	Q99594.
GermOnline	ENSG00000007866. Homo sapiens.
Family and domain databases
InterPro	IPR000818. TEA/ATTS. IPR016361. TEF. IPR027253. TEF-5. [Graphical view]
PANTHER	PTHR11834. PTHR11834. 1 hit.
Pfam	PF01285. TEA. 1 hit. [Graphical view]
PIRSF	PIRSF002603. TEF. 1 hit. PIRSF500720. TEF-5. 1 hit.
PRINTS	PR00065. TEADOMAIN.
SMART	SM00426. TEA. 1 hit. [Graphical view]
PROSITE	PS00554. TEA_1. 1 hit. PS51088. TEA_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	7005.
NextBio	27362.
SOURCE	Search...

Entry information

Entry name

TEAD3_HUMAN

Accession

Primary (citable) accession number: Q99594
Secondary accession number(s): O95910, Q5BJG7, Q8N6Y4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 119 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.