Q99583 (MNT_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Max-binding protein MNT

Alternative name(s):
Class D basic helix-loop-helix protein 3
Short name=bHLHd3
Myc antagonist MNT
Protein ROX

Gene names

Name:	MNT
Synonyms:	BHLHD3, ROX

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	582 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Binds DNA as a heterodimer with MAX and represses transcription. Binds to the canonical E box sequence 5'-CACGTG-3' and, with higher affinity, to 5'-CACGCG-3'.
Subunit structure	Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a homodimer or a heterodimer with MAX.
Subcellular location	Nucleus.
Sequence similarities	Contains 1 bHLH (basic helix-loop-helix) domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Ligand	DNA-binding
Molecular function	Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell aging Inferred from electronic annotation. Source: Compara multicellular organismal development Traceable author statement Ref.1. Source: ProtInc negative regulation of cell proliferation Traceable author statement Ref.1. Source: ProtInc regulation of apoptotic process Inferred from electronic annotation. Source: Compara regulation of cell cycle Inferred from electronic annotation. Source: Compara transcription from RNA polymerase II promoter Traceable author statement Ref.1. Source: ProtInc
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW chromatin binding Inferred from electronic annotation. Source: Compara sequence-specific DNA binding transcription factor activity Traceable author statement Ref.1. Source: ProtInc transcription coactivator activity Traceable author statement Ref.2. Source: ProtInc transcription corepressor activity Traceable author statement Ref.2. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 582	582	Max-binding protein MNT		PRO_0000127281
Regions
	Domain	220 – 271	52	bHLH
	Region	271 – 299	29	Leucine-zipper
Natural variations
	Natural variant	109	1	A → T. Corresponds to variant rs7207965 [ dbSNP | Ensembl ].		VAR_061258

Sequences

	Sequence		Length	Mass (Da)
	Q99583 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 06AC320D79BF18A0	FASTA	582	62,300
	10 20 30 40 50 60 MSIETLLEAA RFLEWQAQQQ QRAREEQERL RLEQEREQEQ KKANSLARLA HTLPVEEPRM 70 80 90 100 110 120 EAPPLPLSPP APPPAPPPPL ATPAPLTVIP IPVVTNSPQP LPPPPPLPAA AQPLPLAPRQ 130 140 150 160 170 180 PALVGAPGLS IKEPAPLPSR PQVPTPAPLL PDSKATIPPN GSPKPLQPLP TPVLTIAPHP 190 200 210 220 230 240 GVQPQLAPQQ PPPPTLGTLK LAPAEEVKSS EQKKRPGGIG TREVHNKLEK NRRAHLKECF 250 260 270 280 290 300 ETLKRNIPNV DDKKTSNLSV LRTALRYIQS LKRKEKEYEH EMERLAREKI ATQQRLAELK 310 320 330 340 350 360 HELSQWMDVL EIDRVLRQTG QPEDDQASTS TASEGEDNID EDMEEDRAGL GPPKLSHRPQ 370 380 390 400 410 420 PELLKSTLPP PSTTPAPLPP HPHPHPHSVA LPPAHLPVQQ QQPQQKTPLP APPPPPAAPA 430 440 450 460 470 480 QTLVPAPAHL VATAGGGSTV IAHTATTHAS VIQTVNHVLQ GPGGKHIAHI APSAPSPAVQ 490 500 510 520 530 540 LAPATPPIGH ITVHPATLNH VAHLGSQLPL YPQPVAVSHI AHTLSHQQVN GTAGLGPPAT 550 560 570 580 VMAKPAVGAQ VVHHPQLVGQ TVLNPVTMVT MPSFPVSTLK LA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Rox, a novel bHLHZip protein expressed in quiescent cells that heterodimerizes with Max, binds a non-canonical E box and acts as a transcriptional repressor." Meroni G., Reymond A., Alcalay M., Borsani G., Tanigami A., Tonlorenzi R., Lo Nigro C., Messali S., Zollo M., Ledbetter D.H., Brent R., Ballabio A., Carrozzo R. EMBO J. 16:2892-2906(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Fetal brain.
[2]	"The human ROX gene: genomic structure and mutation analysis in human breast tumors." Nigro C.L., Venesio T., Reymond A., Meroni G., Alberici P., Cainarca S., Enrico F., Stack M., Ledbetter D.H., Liscia D.S., Ballabio A., Carrozzo R. Genomics 49:275-282(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pancreas.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X96401 mRNA. Translation: CAA65265.1. Y13440 , Y13441, Y13442, Y13443, Y13444 Genomic DNA. Translation: CAA73851.1. AK291596 mRNA. Translation: BAF84285.1. CH471108 Genomic DNA. Translation: EAW90541.1. CH471108 Genomic DNA. Translation: EAW90542.1. BC117563 mRNA. Translation: AAI17564.1.
IPI	IPI00016177.
RefSeq	NP_064706.1. NM_020310.2.
UniGene	Hs.626579. Hs.632239.
3D structure databases
ProteinModelPortal	Q99583.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000174618.
PTM databases
PhosphoSite	Q99583.
Polymorphism databases
DMDM	3914034.
Proteomic databases
PaxDb	Q99583.
PRIDE	Q99583.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000174618; ENSP00000174618; ENSG00000070444. ENST00000404961; ENSP00000384177; ENSG00000070444.
GeneID	4335.
KEGG	hsa:4335.
UCSC	uc002fur.3. human.
Organism-specific databases
CTD	4335.
GeneCards	GC17M002287.
HGNC	HGNC:7188. MNT.
MIM	603039. gene.
neXtProt	NX_Q99583.
PharmGKB	PA30898.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG314808.
HOGENOM	HOG000013115.
HOVERGEN	HBG006379.
InParanoid	Q99583.
KO	K09115.
OMA	AHLPVQQ.
OrthoDB	EOG4WQ130.
PhylomeDB	Q99583.
Gene expression databases
Bgee	Q99583.
CleanEx	HS_MNT.
Genevestigator	Q99583.
GermOnline	ENSG00000070444. Homo sapiens.
Family and domain databases
Gene3D	4.10.280.10. 1 hit.
InterPro	IPR011598. bHLH_dom. [Graphical view]
Pfam	PF00010. HLH. 1 hit. [Graphical view]
SMART	SM00353. HLH. 1 hit. [Graphical view]
SUPFAM	SSF47459. HLH_basic. 1 hit.
PROSITE	PS50888. BHLH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	MNT. human.
GenomeRNAi	4335.
NextBio	17053.
SOURCE	Search...

Entry information

Entry name

MNT_HUMAN

Accession

Primary (citable) accession number: Q99583
Secondary accession number(s): A8K6D1, D3DTI7, Q1ED38

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	May 1, 1997
Last modified:	May 1, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.