Reviewed, UniProtKB/Swiss-Prot Q99578 (RIT2_HUMAN)

Names and origin

Protein names

Recommended name:
    GTP-binding protein Rit2
Alternative name(s):
    Ras-like protein expressed in neurons
    Ras-like without CAAX protein 2

Gene names

Name:	RIT2
Synonyms:	RIN, ROC2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	217 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Binds and exchanges GTP and GDP By similarity.
Subunit structure	Interacts with MLLT4, the C-terminal domain of RALGDS and RLF, but not with RIN1 and PIK3CA. RLF binds exclusively to the active GTP-bound form By similarity. Binds calmodulin.
Subcellular location	Cell membrane.
Tissue specificity	Neuron-specific.
Miscellaneous	Shows rapid uncatalyzed guanine nucleotide dissociation rates, which are much faster than those of most Ras subfamily members By similarity.
Sequence similarities	Belongs to the small GTPase superfamily. Ras family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Ligand	Calmodulin-binding GTP-binding Nucleotide-binding
Gene Ontology (GO)
Biological process	small GTPase mediated signal transduction Ref.1 Non-traceable author statement. Source: UniProtKB synaptic transmission Ref.2 Traceable author statement. Source: ProtInc
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro plasma membrane Ref.1 Traceable author statement. Source: UniProtKB
Molecular function	GTP binding Ref.1 Traceable author statement. Source: ProtInc GTPase activity Ref.1 Ref.2 Non-traceable author statement. Source: UniProtKB calmodulin binding Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GRB2	P62993	1	EBI-365914,EBI-401755
RGL1	Q9NZL6	1	EBI-365914,EBI-365926

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q99578-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q99578-2)

Also known as: RIBA;

The sequence of this isoform differs from the canonical sequence as follows:
     143-153: VSTEEGLSLAQ → PTLKEFGREEF
     154-217: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 217	217	GTP-binding protein Rit2		PRO_0000082727
Regions
	Nucleotide binding	27 – 34	8	GTP By similarity
	Nucleotide binding	74 – 78	5	GTP By similarity
	Nucleotide binding	133 – 136	4	GTP By similarity
Natural variations
	Alternative sequence	143 – 153	11	VSTEEGLSLAQ → PTLKEFGREEF in isoform 2.		VSP_018102
	Alternative sequence	154 – 217	64	Missing in isoform 2.		VSP_018103
Experimental info
	Sequence conflict	39	1	Q → L in AAM12636. Ref.4
	Sequence conflict	76	1	A → D in AAB42214. Ref.1
	Sequence conflict	200	1	S → C in AAH18060. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: CD2A9ECE4AEAA792	FASTA	217	24,668
	10 20 30 40 50 60 MEVENEASCS PGSASGGSRE YKVVMLGAGG VGKSAMTMQF ISHQFPDYHD PTIEDAYKTQ 70 80 90 100 110 120 VRIDNEPAYL DILDTAGQAE FTAMREQYMR GGEGFIICYS VTDRQSFQEA AKFKELIFQV 130 140 150 160 170 180 RHTYEIPLVL VGNKIDLEQF RQVSTEEGLS LAQEYNCGFF ETSAALRFCI DDAFHGLVRE 190 200 210 IRKKESMPSL MEKKLKRKDS LWKKLKGSLK KKRENMT
	Isoform 2 (RIBA). Checksum: 9820326EF5FCA223	FASTA	153	17,308
	10 20 30 40 50 60 MEVENEASCS PGSASGGSRE YKVVMLGAGG VGKSAMTMQF ISHQFPDYHD PTIEDAYKTQ 70 80 90 100 110 120 VRIDNEPAYL DILDTAGQAE FTAMREQYMR GGEGFIICYS VTDRQSFQEA AKFKELIFQV 130 140 150 RHTYEIPLVL VGNKIDLEQF RQPTLKEFGR EEF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Rin, a neuron-specific and calmodulin-binding small G-protein, and Rit define a novel subfamily of ras proteins." Lee C.H.J., Della N.G., Chew C.E., Zack D.J. J. Neurosci. 16:6784-6794(1996) [PubMed: 8824319] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CHARACTERIZATION.
[2]	"RIC, a calmodulin-binding Ras-like GTPase." Wes P.D., Yu M., Montell C. EMBO J. 15:5839-5848(1996) [PubMed: 8918462] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"Characterization of new small G proteins in the Ras subfamily." Kawasaki H., Housman D.E., Graybiel A.M. Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). Tissue: Neuron.
[4]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U71204 mRNA. Translation: AAB42214.1. Y07565 mRNA. Translation: CAA68850.1. U78164 mRNA. Translation: AAB64245.1. U78166 mRNA. Translation: AAB64247.1. AF493922 mRNA. Translation: AAM12636.1. AY563951 mRNA. Translation: AAS75332.1. BC018060 mRNA. Translation: AAH18060.1.
IPI	IPI00163763. IPI00289566.
RefSeq	NP_002921.1.
UniGene	Hs.464985
3D structure databases
HSSP	HSSP built from PDB template 1IOZ based on UniProtKB P01112.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q99578. 4 interactions.
Proteomic databases
PRIDE	Q99578.
Genome annotation databases
Ensembl	ENSG00000152214. Homo sapiens. [Contig view]
GeneID	6014.
KEGG	hsa:6014.
Organism-specific databases
GeneCards	GC18M038577.
HGNC	HGNC:10017. RIT2.
MIM	609592. gene.
PharmGKB	PA35527.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q99578.
HOVERGEN	Q99578.
OMA	Q99578. KDSLWRK.
Enzyme and pathway databases
Pathway_Interaction_DB	trkrpathway. Neurotrophic factor-mediated Trk receptor signaling. mapktrkpathway. Trk receptor signaling mediated by the MAPK pathway.
Reactome	REACT_11061. Signalling by NGF.
Gene expression databases
ArrayExpress	Q99578.
Bgee	Q99578.
CleanEx	HS_RIT2.
GermOnline	ENSG00000152214. Homo sapiens.
Family and domain databases
InterPro	IPR003577. GTPase_Ras. IPR013753. Ras. IPR001806. Ras_GTPase. IPR005225. Small_GTP_bd. [Graphical view]
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00173. RAS. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS51421. RAS. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	23463.
SOURCE	Search...

Entry information

Entry name

RIT2_HUMAN

Accession

Primary (citable) accession number: Q99578
Secondary accession number(s): O15295 , Q8TD69, Q8WVF6, Q92964

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2003
Last sequence update:	May 1, 1997
Last modified:	June 16, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)