Q99571 (P2RX4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
P2X purinoceptor 4
Short name=P2X4

Alternative name(s):
ATP receptor
Purinergic receptor

Gene names

Name:

P2RX4

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for ATP that acts as a ligand-gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin.
Subunit structure	Functional P2XRs are organized as homomeric and heteromeric trimers By similarity.
Subcellular location	Membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the P2X receptor family.

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	Ion channel Ligand-gated ion channel Receptor
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	calcium ion transport Inferred from electronic annotation. Source: Compara endothelial cell activation Traceable author statement PubMed 12088286. Source: BHF-UCL membrane depolarization Inferred from direct assay Ref.1. Source: BHF-UCL negative regulation of cardiac muscle hypertrophy Inferred from mutant phenotype PubMed 15130891. Source: BHF-UCL nitric oxide biosynthetic process Inferred from electronic annotation. Source: Compara positive regulation of calcium ion transport into cytosol Inferred from direct assay PubMed 10969036. Source: BHF-UCL positive regulation of calcium-mediated signaling Inferred from direct assay PubMed 10969036. Source: BHF-UCL positive regulation of nitric oxide biosynthetic process Non-traceable author statement PubMed 10899068. Source: BHF-UCL positive regulation of prostaglandin secretion Non-traceable author statement PubMed 10899068. Source: BHF-UCL protein homooligomerization Inferred from electronic annotation. Source: Compara regulation of action potential in neuron Inferred from electronic annotation. Source: Compara regulation of apoptotic process Inferred from direct assay PubMed 17264311. Source: BHF-UCL regulation of blood pressure Inferred from mutant phenotype PubMed 11606481PubMed 15130891. Source: BHF-UCL regulation of cardiac muscle contraction Inferred from mutant phenotype PubMed 11606481PubMed 15130891. Source: BHF-UCL regulation of excitatory postsynaptic membrane potential Inferred from electronic annotation. Source: Compara regulation of sodium ion transport Inferred from sequence or structural similarity PubMed 17785580. Source: BHF-UCL relaxation of cardiac muscle Inferred from mutant phenotype PubMed 11606481PubMed 15130891. Source: BHF-UCL response to ATP Inferred from direct assay Ref.1. Source: BHF-UCL response to fluid shear stress Inferred from direct assay PubMed 10969036. Source: BHF-UCL sensory perception of pain Inferred from sequence or structural similarity PubMed 14978347. Source: BHF-UCL tissue homeostasis Non-traceable author statement PubMed 17264311. Source: BHF-UCL vasodilation Inferred from electronic annotation. Source: Compara
Cellular_component	apical part of cell Inferred from electronic annotation. Source: Compara cell junction Inferred from direct assay PubMed 12088286. Source: BHF-UCL integral to plasma membrane Inferred by curator PubMed 11606481. Source: BHF-UCL neuronal cell body Inferred from electronic annotation. Source: Compara perinuclear region of cytoplasm Inferred from direct assay PubMed 12088286. Source: BHF-UCL postsynaptic density Inferred from electronic annotation. Source: Compara terminal bouton Inferred from electronic annotation. Source: Compara
Molecular_function	ATP binding Inferred by curator PubMed 11606481. Source: BHF-UCL copper ion binding Inferred from sequence or structural similarity PubMed 12819199. Source: BHF-UCL drug binding Inferred from electronic annotation. Source: Compara extracellular ATP-gated cation channel activity Inferred from direct assay Ref.1. Source: BHF-UCL protein homodimerization activity Non-traceable author statement PubMed 17895406. Source: BHF-UCL purinergic nucleotide receptor activity Inferred from direct assay Ref.1. Source: BHF-UCL receptor binding Inferred from sequence or structural similarity PubMed 17785580. Source: BHF-UCL zinc ion binding Inferred from sequence or structural similarity PubMed 12819199. Source: BHF-UCL
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 388	388	P2X purinoceptor 4		PRO_0000161553
Regions
	Topological domain	1 – 33	33	Cytoplasmic Potential
	Transmembrane	34 – 54	21	Helical; Name=1; Potential
	Topological domain	55 – 338	284	Extracellular Potential
	Transmembrane	339 – 359	21	Helical; Name=2; Potential
	Topological domain	360 – 388	29	Cytoplasmic Potential
Amino acid modifications
	Modified residue	378	1	Phosphotyrosine By similarity
	Glycosylation	75	1	N-linked (GlcNAc...) Potential
	Glycosylation	110	1	N-linked (GlcNAc...) Potential
	Glycosylation	153	1	N-linked (GlcNAc...) Potential
	Glycosylation	184	1	N-linked (GlcNAc...) Ref.8
	Glycosylation	199	1	N-linked (GlcNAc...) Potential
	Glycosylation	208	1	N-linked (GlcNAc...) Potential
	Disulfide bond	116 ↔ 165		By similarity
	Disulfide bond	126 ↔ 149		By similarity
	Disulfide bond	132 ↔ 159		By similarity
	Disulfide bond	217 ↔ 227		By similarity
	Disulfide bond	261 ↔ 270		By similarity
Natural variations
	Natural variant	6	1	A → S. Ref.1 Ref.3 Ref.4 Corresponds to variant rs1044249 [ dbSNP | Ensembl ].		VAR_028307
	Natural variant	242	1	S → G. Ref.5 Corresponds to variant rs25644 [ dbSNP | Ensembl ].		VAR_014942
Experimental info
	Sequence conflict	121	1	D → S in AAB66834. Ref.7
	Sequence conflict	148	1	R → W in AAB66834. Ref.7
	Sequence conflict	179	1	L → F in AAB66834. Ref.7
	Sequence conflict	238	1	N → I in AAB66834. Ref.7
	Sequence conflict	315	1	Y → C in AAB58405. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q99571 [UniParc]. Last modified October 17, 2006. Version 2. Checksum: BA3BE7F30114C2A5	FASTA	388	43,369
	10 20 30 40 50 60 MAGCCAALAA FLFEYDTPRI VLIRSRKVGL MNRAVQLLIL AYVIGWVFVW EKGYQETDSV 70 80 90 100 110 120 VSSVTTKVKG VAVTNTSKLG FRIWDVADYV IPAQEENSLF VMTNVILTMN QTQGLCPEIP 130 140 150 160 170 180 DATTVCKSDA SCTAGSAGTH SNGVSTGRCV AFNGSVKTCE VAAWCPVEDD THVPQPAFLK 190 200 210 220 230 240 AAENFTLLVK NNIWYPKFNF SKRNILPNIT TTYLKSCIYD AKTDPFCPIF RLGKIVENAG 250 260 270 280 290 300 HSFQDMAVEG GIMGIQVNWD CNLDRAASLC LPRYSFRRLD TRDVEHNVSP GYNFRFAKYY 310 320 330 340 350 360 RDLAGNEQRT LIKAYGIRFD IIVFGKAGKF DIIPTMINIG SGLALLGMAT VLCDIIVLYC 370 380 MKKRLYYREK KYKYVEDYEQ GLASELDQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of recombinant human P2X4 receptor reveals pharmacological differences to the rat homologue." Garcia-Guzman M., Soto F., Gomez-Hernandez J.M., Lund P.E., Stuhmer W. Mol. Pharmacol. 51:109-118(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT SER-6. Tissue: Brain.
[2]	"Transcription map of the 5cM region surrounding the hepatocyte nuclear factor-1a/MODY3 gene on chromosome 12." Yamagata K., Oda N., Furuta H., Vaxillaire M., Southam L., Boriraj V., Chen X., Oda Y., Takeda J., Yamada S., Nishigori H., Lebeau M.M., Lathrop M., Cox R.D., Bell G.I. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[3]	"Shear stress downregulates the expression of P2X4 receptor by human endothelial cells." Korenaga R., Yamamoto K., Kamiya A., Ando J. Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT SER-6.
[4]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT SER-6.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLY-242. Tissue: Blood.
[6]	"Human P2X purinoceptor." Takahashi K., Korenaga R., Kamiya A., Ando J. Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 35-388.
[7]	"Cloning of P2X4 cDNA from human embryonic kidney (HEK) 293 cells." Chang A.S., Chang S.M. Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 73-326. Tissue: Kidney.
[8]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-184, MASS SPECTROMETRY. Tissue: Liver.

Web resources

Wikipedia

P2X receptor entry

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y07684 mRNA. Translation: CAA68948.1. U87270 , U85971, U85972, U85973, U85974, U85975 Genomic DNA. Translation: AAD00556.1. U83993 mRNA. Translation: AAD00553.1. AF191093 Genomic DNA. Translation: AAF06661.1. BT019738 mRNA. Translation: AAV38543.1. BC033826 mRNA. Translation: AAH33826.1. AF000234 mRNA. Translation: AAB58405.1. AF012903 mRNA. Translation: AAB66834.1.
IPI	IPI00293327.
RefSeq	NP_001243725.1. NM_001256796.1. NP_001248326.1. NM_001261397.1. NP_002551.2. NM_002560.2.
UniGene	Hs.321709.
3D structure databases
ProteinModelPortal	Q99571.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q99571. 3 interactions.
STRING	9606.ENSP00000336607.
PTM databases
PhosphoSite	Q99571.
Polymorphism databases
DMDM	116242696.
Proteomic databases
PaxDb	Q99571.
PRIDE	Q99571.
Protocols and materials databases
DNASU	5025.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000337233; ENSP00000336607; ENSG00000135124.
GeneID	5025.
KEGG	hsa:5025.
UCSC	uc001tzr.3. human.
Organism-specific databases
CTD	5025.
GeneCards	GC12P121647.
HGNC	HGNC:8535. P2RX4.
HPA	HPA039494.
MIM	600846. gene.
neXtProt	NX_Q99571.
PharmGKB	PA32864.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG47843.
HOGENOM	HOG000232042.
HOVERGEN	HBG053086.
InParanoid	Q99571.
KO	K05218.
OrthoDB	EOG418BNK.
PhylomeDB	Q99571.
Gene expression databases
ArrayExpress	Q99571.
Bgee	Q99571.
CleanEx	HS_P2RX4.
Genevestigator	Q99571.
GermOnline	ENSG00000135124. Homo sapiens.
Family and domain databases
Gene3D	2.60.490.10. 1 hit.
InterPro	IPR003047. P2X4_purnocptor. IPR027309. P2X_extracellular_dom. IPR001429. P2X_purnocptor. [Graphical view]
PANTHER	PTHR10125. PTHR10125. 1 hit. PTHR10125:SF18. PTHR10125:SF18. 1 hit.
Pfam	PF00864. P2X_receptor. 1 hit. [Graphical view]
PRINTS	PR01311. P2X4RECEPTOR. PR01307. P2XRECEPTOR.
TIGRFAMs	TIGR00863. P2X. 1 hit.
PROSITE	PS01212. P2X_RECEPTOR. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q99571.
ChEMBL	CHEMBL2104.
ChiTaRS	P2RX4. human.
GenomeRNAi	5025.
NextBio	19346.
SOURCE	Search...

Entry information

Entry name

P2RX4_HUMAN

Accession

Primary (citable) accession number: Q99571
Secondary accession number(s): O00450 , O14722, Q5U090, Q8N4N1, Q9UBG9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	October 17, 2006
Last modified:	May 1, 2013
This is version 119 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.