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Protein

P2X purinoceptor 4

Gene

P2RX4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for ATP that acts as a ligand-gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin.1 Publication

GO - Molecular functioni

  • ATP binding Source: BHF-UCL
  • cadherin binding Source: BHF-UCL
  • copper ion binding Source: BHF-UCL
  • drug binding Source: Ensembl
  • extracellular ATP-gated cation channel activity Source: UniProtKB
  • protein homodimerization activity Source: BHF-UCL
  • purinergic nucleotide receptor activity Source: BHF-UCL
  • receptor binding Source: BHF-UCL
  • zinc ion binding Source: BHF-UCL

GO - Biological processi

  • apoptotic signaling pathway Source: BHF-UCL
  • blood coagulation Source: Reactome
  • cellular response to ATP Source: UniProtKB
  • endothelial cell activation Source: BHF-UCL
  • ion transmembrane transport Source: UniProtKB
  • membrane depolarization Source: BHF-UCL
  • negative regulation of cardiac muscle hypertrophy Source: BHF-UCL
  • neuronal action potential Source: Ensembl
  • positive regulation of calcium ion transport Source: BHF-UCL
  • positive regulation of calcium ion transport into cytosol Source: BHF-UCL
  • positive regulation of calcium-mediated signaling Source: BHF-UCL
  • positive regulation of nitric oxide biosynthetic process Source: BHF-UCL
  • positive regulation of prostaglandin secretion Source: BHF-UCL
  • protein homooligomerization Source: Ensembl
  • purinergic nucleotide receptor signaling pathway Source: BHF-UCL
  • regulation of blood pressure Source: BHF-UCL
  • regulation of cardiac muscle contraction Source: BHF-UCL
  • regulation of sodium ion transport Source: BHF-UCL
  • relaxation of cardiac muscle Source: BHF-UCL
  • response to ATP Source: BHF-UCL
  • response to fluid shear stress Source: BHF-UCL
  • sensory perception of pain Source: BHF-UCL
  • signal transduction Source: BHF-UCL
  • tissue homeostasis Source: BHF-UCL
  • transport Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Enzyme and pathway databases

BioCyciZFISH:ENSG00000135124-MONOMER.
ReactomeiR-HSA-139853. Elevation of cytosolic Ca2+ levels.
R-HSA-418346. Platelet homeostasis.

Protein family/group databases

TCDBi1.A.7.1.5. the atp-gated p2x receptor cation channel (p2x receptor) family.

Names & Taxonomyi

Protein namesi
Recommended name:
P2X purinoceptor 4
Short name:
P2X4
Alternative name(s):
ATP receptor
Purinergic receptor
Gene namesi
Name:P2RX4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:8535. P2RX4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 33CytoplasmicSequence analysisAdd BLAST33
Transmembranei34 – 54Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini55 – 338ExtracellularSequence analysisAdd BLAST284
Transmembranei339 – 359Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini360 – 388CytoplasmicSequence analysisAdd BLAST29

GO - Cellular componenti

  • cell junction Source: BHF-UCL
  • dendritic spine Source: Ensembl
  • extracellular exosome Source: UniProtKB
  • integral component of nuclear inner membrane Source: GO_Central
  • integral component of plasma membrane Source: UniProtKB
  • lysosomal membrane Source: UniProtKB
  • membrane Source: UniProtKB
  • neuronal cell body Source: Ensembl
  • perinuclear region of cytoplasm Source: BHF-UCL
  • plasma membrane Source: BHF-UCL
  • postsynaptic density Source: Ensembl
  • terminal bouton Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi5025.
OpenTargetsiENSG00000135124.
PharmGKBiPA32864.

Chemistry databases

ChEMBLiCHEMBL2104.
DrugBankiDB09119. Eslicarbazepine acetate.
GuidetoPHARMACOLOGYi481.

Polymorphism and mutation databases

BioMutaiP2RX4.
DMDMi116242696.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001615531 – 388P2X purinoceptor 4Add BLAST388

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi75N-linked (GlcNAc...)Sequence analysis1
Glycosylationi110N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi116 ↔ 165By similarity
Disulfide bondi126 ↔ 149By similarity
Disulfide bondi132 ↔ 159By similarity
Glycosylationi153N-linked (GlcNAc...)Sequence analysis1
Glycosylationi184N-linked (GlcNAc...)1 Publication1
Glycosylationi199N-linked (GlcNAc...)Sequence analysis1
Glycosylationi208N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi217 ↔ 227By similarity
Disulfide bondi261 ↔ 270By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ99571.
PaxDbiQ99571.
PeptideAtlasiQ99571.
PRIDEiQ99571.

PTM databases

iPTMnetiQ99571.
PhosphoSitePlusiQ99571.

Expressioni

Gene expression databases

BgeeiENSG00000135124.
CleanExiHS_P2RX4.
ExpressionAtlasiQ99571. baseline and differential.
GenevisibleiQ99571. HS.

Organism-specific databases

HPAiHPA039494.

Interactioni

Subunit structurei

Functional P2XRs are organized as homomeric and heteromeric trimers.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
KRTAP5-9P263713EBI-2828248,EBI-3958099
NOTCH2NLQ7Z3S95EBI-2828248,EBI-945833
PIH1D3Q9NQM43EBI-2828248,EBI-10239299

GO - Molecular functioni

  • cadherin binding Source: BHF-UCL
  • protein homodimerization activity Source: BHF-UCL
  • receptor binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi111064. 31 interactors.
IntActiQ99571. 8 interactors.
STRINGi9606.ENSP00000336607.

Chemistry databases

BindingDBiQ99571.

Structurei

3D structure databases

ProteinModelPortaliQ99571.
SMRiQ99571.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the P2X receptor family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFJF. Eukaryota.
ENOG410XR0C. LUCA.
GeneTreeiENSGT00390000016028.
HOGENOMiHOG000232042.
HOVERGENiHBG053086.
InParanoidiQ99571.
KOiK05218.
OMAiTMINVGS.
OrthoDBiEOG091G0A2U.
PhylomeDBiQ99571.
TreeFamiTF328633.

Family and domain databases

Gene3Di2.60.490.10. 1 hit.
InterProiIPR003047. P2X4_purnocptor.
IPR027309. P2X_extracellular_dom.
IPR001429. P2X_purnocptor.
[Graphical view]
PANTHERiPTHR10125. PTHR10125. 1 hit.
PTHR10125:SF18. PTHR10125:SF18. 1 hit.
PfamiPF00864. P2X_receptor. 1 hit.
[Graphical view]
PIRSFiPIRSF005713. P2X_purinoceptor. 1 hit.
PRINTSiPR01311. P2X4RECEPTOR.
PR01307. P2XRECEPTOR.
TIGRFAMsiTIGR00863. P2X. 1 hit.
PROSITEiPS01212. P2X_RECEPTOR. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q99571-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGCCAALAA FLFEYDTPRI VLIRSRKVGL MNRAVQLLIL AYVIGWVFVW
60 70 80 90 100
EKGYQETDSV VSSVTTKVKG VAVTNTSKLG FRIWDVADYV IPAQEENSLF
110 120 130 140 150
VMTNVILTMN QTQGLCPEIP DATTVCKSDA SCTAGSAGTH SNGVSTGRCV
160 170 180 190 200
AFNGSVKTCE VAAWCPVEDD THVPQPAFLK AAENFTLLVK NNIWYPKFNF
210 220 230 240 250
SKRNILPNIT TTYLKSCIYD AKTDPFCPIF RLGKIVENAG HSFQDMAVEG
260 270 280 290 300
GIMGIQVNWD CNLDRAASLC LPRYSFRRLD TRDVEHNVSP GYNFRFAKYY
310 320 330 340 350
RDLAGNEQRT LIKAYGIRFD IIVFGKAGKF DIIPTMINIG SGLALLGMAT
360 370 380
VLCDIIVLYC MKKRLYYREK KYKYVEDYEQ GLASELDQ
Length:388
Mass (Da):43,369
Last modified:October 17, 2006 - v2
Checksum:iBA3BE7F30114C2A5
GO
Isoform 2 (identifier: Q99571-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     45-45: G → GCYHPHLAEVEMESPRR

Note: No experimental confirmation available.
Show »
Length:404
Mass (Da):45,305
Checksum:i0A8CB40D3D899F21
GO
Isoform 3 (identifier: Q99571-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     149-175: Missing.

Show »
Length:361
Mass (Da):40,481
Checksum:i9D09FA497870DDB1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti121D → S in AAB66834 (Ref. 8) Curated1
Sequence conflicti148R → W in AAB66834 (Ref. 8) Curated1
Sequence conflicti179L → F in AAB66834 (Ref. 8) Curated1
Sequence conflicti238N → I in AAB66834 (Ref. 8) Curated1
Sequence conflicti315Y → C in AAB58405 (Ref. 7) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0283076A → S.3 PublicationsCorresponds to variant rs1044249dbSNPEnsembl.1
Natural variantiVAR_014942242S → G.1 PublicationCorresponds to variant rs25644dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05381245G → GCYHPHLAEVEMESPRR in isoform 2. Curated1
Alternative sequenceiVSP_053813149 – 175Missing in isoform 3. 1 PublicationAdd BLAST27

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y07684 mRNA. Translation: CAA68948.1.
U87270
, U85971, U85972, U85973, U85974, U85975 Genomic DNA. Translation: AAD00556.1.
U83993 mRNA. Translation: AAD00553.1.
AF191093 Genomic DNA. Translation: AAF06661.1.
BT019738 mRNA. Translation: AAV38543.1.
BT019739 mRNA. Translation: AAV38544.1.
AC069209 Genomic DNA. No translation available.
BC033826 mRNA. Translation: AAH33826.1.
AF000234 mRNA. Translation: AAB58405.1.
AF012903 mRNA. Translation: AAB66834.1.
CCDSiCCDS58282.1. [Q99571-2]
CCDS9214.1. [Q99571-1]
RefSeqiNP_001243725.1. NM_001256796.1. [Q99571-2]
NP_001248326.1. NM_001261397.1. [Q99571-3]
NP_002551.2. NM_002560.2. [Q99571-1]
UniGeneiHs.321709.

Genome annotation databases

EnsembliENST00000337233; ENSP00000336607; ENSG00000135124. [Q99571-1]
ENST00000359949; ENSP00000353032; ENSG00000135124. [Q99571-2]
ENST00000542067; ENSP00000438329; ENSG00000135124. [Q99571-3]
GeneIDi5025.
KEGGihsa:5025.
UCSCiuc001tzr.4. human. [Q99571-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

Wikipedia

P2X receptor entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y07684 mRNA. Translation: CAA68948.1.
U87270
, U85971, U85972, U85973, U85974, U85975 Genomic DNA. Translation: AAD00556.1.
U83993 mRNA. Translation: AAD00553.1.
AF191093 Genomic DNA. Translation: AAF06661.1.
BT019738 mRNA. Translation: AAV38543.1.
BT019739 mRNA. Translation: AAV38544.1.
AC069209 Genomic DNA. No translation available.
BC033826 mRNA. Translation: AAH33826.1.
AF000234 mRNA. Translation: AAB58405.1.
AF012903 mRNA. Translation: AAB66834.1.
CCDSiCCDS58282.1. [Q99571-2]
CCDS9214.1. [Q99571-1]
RefSeqiNP_001243725.1. NM_001256796.1. [Q99571-2]
NP_001248326.1. NM_001261397.1. [Q99571-3]
NP_002551.2. NM_002560.2. [Q99571-1]
UniGeneiHs.321709.

3D structure databases

ProteinModelPortaliQ99571.
SMRiQ99571.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111064. 31 interactors.
IntActiQ99571. 8 interactors.
STRINGi9606.ENSP00000336607.

Chemistry databases

BindingDBiQ99571.
ChEMBLiCHEMBL2104.
DrugBankiDB09119. Eslicarbazepine acetate.
GuidetoPHARMACOLOGYi481.

Protein family/group databases

TCDBi1.A.7.1.5. the atp-gated p2x receptor cation channel (p2x receptor) family.

PTM databases

iPTMnetiQ99571.
PhosphoSitePlusiQ99571.

Polymorphism and mutation databases

BioMutaiP2RX4.
DMDMi116242696.

Proteomic databases

MaxQBiQ99571.
PaxDbiQ99571.
PeptideAtlasiQ99571.
PRIDEiQ99571.

Protocols and materials databases

DNASUi5025.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000337233; ENSP00000336607; ENSG00000135124. [Q99571-1]
ENST00000359949; ENSP00000353032; ENSG00000135124. [Q99571-2]
ENST00000542067; ENSP00000438329; ENSG00000135124. [Q99571-3]
GeneIDi5025.
KEGGihsa:5025.
UCSCiuc001tzr.4. human. [Q99571-1]

Organism-specific databases

CTDi5025.
DisGeNETi5025.
GeneCardsiP2RX4.
HGNCiHGNC:8535. P2RX4.
HPAiHPA039494.
MIMi600846. gene.
neXtProtiNX_Q99571.
OpenTargetsiENSG00000135124.
PharmGKBiPA32864.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IFJF. Eukaryota.
ENOG410XR0C. LUCA.
GeneTreeiENSGT00390000016028.
HOGENOMiHOG000232042.
HOVERGENiHBG053086.
InParanoidiQ99571.
KOiK05218.
OMAiTMINVGS.
OrthoDBiEOG091G0A2U.
PhylomeDBiQ99571.
TreeFamiTF328633.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000135124-MONOMER.
ReactomeiR-HSA-139853. Elevation of cytosolic Ca2+ levels.
R-HSA-418346. Platelet homeostasis.

Miscellaneous databases

ChiTaRSiP2RX4. human.
GeneWikiiP2RX4.
GenomeRNAii5025.
PROiQ99571.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000135124.
CleanExiHS_P2RX4.
ExpressionAtlasiQ99571. baseline and differential.
GenevisibleiQ99571. HS.

Family and domain databases

Gene3Di2.60.490.10. 1 hit.
InterProiIPR003047. P2X4_purnocptor.
IPR027309. P2X_extracellular_dom.
IPR001429. P2X_purnocptor.
[Graphical view]
PANTHERiPTHR10125. PTHR10125. 1 hit.
PTHR10125:SF18. PTHR10125:SF18. 1 hit.
PfamiPF00864. P2X_receptor. 1 hit.
[Graphical view]
PIRSFiPIRSF005713. P2X_purinoceptor. 1 hit.
PRINTSiPR01311. P2X4RECEPTOR.
PR01307. P2XRECEPTOR.
TIGRFAMsiTIGR00863. P2X. 1 hit.
PROSITEiPS01212. P2X_RECEPTOR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiP2RX4_HUMAN
AccessioniPrimary (citable) accession number: Q99571
Secondary accession number(s): E7EPF7
, F6RU17, O00450, O14722, Q5U089, Q5U090, Q8N4N1, Q9UBG9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: October 17, 2006
Last modified: November 30, 2016
This is version 156 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.