Q99567 (NUP88_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nuclear pore complex protein Nup88

Alternative name(s):
88 kDa nucleoporin
Nucleoporin Nup88

Gene names

Name:

NUP88

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	741 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential component of nuclear pore complex.
Subunit structure	Interacts with NUP214/CAN. Ref.1
Subcellular location	Nucleus › nuclear pore complex.
Tissue specificity	Ubiquitous.
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.4 Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10

Ontologies

Keywords
Biological process	Protein transport Translocation Transport mRNA transport
Cellular component	Nuclear pore complex Nucleus
Coding sequence diversity	Polymorphism
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Traceable author statement. Source: Reactome glucose transport Traceable author statement. Source: Reactome mRNA transport Inferred from electronic annotation. Source: UniProtKB-KW protein transport Inferred from electronic annotation. Source: UniProtKB-KW regulation of glucose transport Traceable author statement. Source: Reactome transmembrane transport Traceable author statement. Source: Reactome viral reproduction Traceable author statement. Source: Reactome
Cellular component	cytosol Traceable author statement. Source: Reactome nuclear pore Traceable author statement. Source: ProtInc
Molecular function	transporter activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 741	741	Nuclear pore complex protein Nup88		PRO_0000204887
Regions
	Coiled coil	585 – 651	67	Potential
Amino acid modifications
	Modified residue	35	1	Phosphoserine Ref.7
	Modified residue	45	1	Phosphoserine Ref.8
	Modified residue	50	1	Phosphoserine Ref.7 Ref.8
	Modified residue	96	1	Phosphoserine Ref.6
	Modified residue	442	1	Phosphoserine Ref.7 Ref.9
	Modified residue	517	1	Phosphoserine Ref.4 Ref.5 Ref.7 Ref.8 Ref.10
	Modified residue	525	1	Phosphothreonine Ref.5 Ref.7 Ref.8 Ref.10
	Modified residue	540	1	Phosphoserine Ref.8
Natural variations
	Natural variant	289	1	N → S. Corresponds to variant rs1806245 [ dbSNP | Ensembl ].		VAR_029340
Experimental info
	Sequence conflict	247	1	A → D in CAA69904. Ref.1
	Sequence conflict	291 – 301	11	GKLLGPLPMHP → WKAVGSIAHAS in CAA69904. Ref.1
	Sequence conflict	456	1	K → R in CAA69904. Ref.1
	Sequence conflict	518	1	P → S in CAA69904. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q99567 [UniParc]. Last modified November 8, 2002. Version 2. Checksum: 954A8E2E203BC20B	FASTA	741	83,542
	10 20 30 40 50 60 MAAAEGPVGD GELWQTWLPN HVVFLRLREG LKNQSPTEAE KPASSSLPSS PPPQLLTRNV 70 80 90 100 110 120 VFGLGGELFL WDGEDSSFLV VRLRGPSGGG EEPALSQYQR LLCINPPLFE IYQVLLSPTQ 130 140 150 160 170 180 HHVALIGIKG LMVLELPKRW GKNSEFEGGK STVNCSTTPV AERFFTSSTS LTLKHAAWYP 190 200 210 220 230 240 SEILDPHVVL LTSDNVIRIY SLREPQTPTN VIILSEAEEE SLVLNKGRAY TASLGETAVA 250 260 270 280 290 300 FDFGPLAAVP KTLFGQNGKD EVVAYPLYIL YENGETFLTY ISLLHSPGNI GKLLGPLPMH 310 320 330 340 350 360 PAAEDNYGYD ACAVLCLPCV PNILVIATES GMLYHCVVLE GEEEDDHTSE KSWDSRIDLI 370 380 390 400 410 420 PSLYVFECVE LELALKLASG EDDPFDSDFS CPVKLHRDPK CPSRYHCTHE AGVHSVGLTW 430 440 450 460 470 480 IHKLHKFLGS DEEDKDSLQE LSTEQKCFVE HILCTKPLPC RQPAPIRGFW IVPDILGPTM 490 500 510 520 530 540 ICITSTYECL IWPLLSTVHP ASPPLLCTRE DVEVAESPLR VLAETPDSFE KHIRSILQRS 550 560 570 580 590 600 VANPAFLKAS EKDIAPPPEE CLQLLSRATQ VFREQYILKQ DLAKEEIQRR VKLLCDQKKK 610 620 630 640 650 660 QLEDLSYCRE ERKSLREMAE RLADKYEEAK EKQEDIMNRM KKLLHSFHSE LPVLSDSERD 670 680 690 700 710 720 MKKELQLIPD QLRHLGNAIK QVTMKKDYQQ QKMEKVLSLP KPTIILSAYQ RKCIQSILKE 730 740 EGEHIREMVK QINDIRNHVN F

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The human homologue of yeast CRM1 is in a dynamic subcomplex with CAN/Nup214 and the novel nuclear pore component Nup88." Fornerod M., van Deursen J.M., van Baal S., Reynolds A., Davis D., Murti K.G., Fransen J., Grosveld G. EMBO J. 16:807-816(1997) [PubMed: 9049309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH NUP214/CAN. Tissue: Placenta.
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[4]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-517, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[5]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed: 16964243] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-517 AND THR-525, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[6]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-96, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[7]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-35; SER-50; SER-442; SER-517 AND THR-525, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45; SER-50; SER-517; THR-525 AND SER-540, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-442, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[10]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-517 AND THR-525, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[11]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y08612 mRNA. Translation: CAA69904.1. CH471108 Genomic DNA. Translation: EAW90340.1. CH471108 Genomic DNA. Translation: EAW90342.1. BC000335 mRNA. Translation: AAH00335.1.
IPI	IPI00001738.
RefSeq	NP_002523.2. NM_002532.4.
UniGene	Hs.584784.
3D structure databases
ProteinModelPortal	Q99567.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q99567. 10 interactions.
MINT	MINT-191368.
STRING	Q99567.
PTM databases
PhosphoSite	Q99567.
Polymorphism databases
DMDM	25008854.
Proteomic databases
PeptideAtlas	Q99567.
PRIDE	Q99567.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000225696; ENSP00000225696; ENSG00000108559.
GeneID	4927.
KEGG	hsa:4927.
NMPDR	fig|9606.3.peg.12953.
UCSC	uc002gbo.1. human.
Organism-specific databases
CTD	4927.
GeneCards	GC17M005230.
H-InvDB	HIX0013467.
HGNC	HGNC:8067. NUP88.
HPA	CAB002209. HPA021816.
MIM	602552. gene.
neXtProt	NX_Q99567.
PharmGKB	PA31855.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG19089.
GeneTree	ENSGT00390000015063.
HOGENOM	HBG357280.
HOVERGEN	HBG052700.
InParanoid	Q99567.
OMA	PLYILYE.
OrthoDB	EOG43R3M9.
PhylomeDB	Q99567.
Enzyme and pathway databases
Reactome	REACT_15518. Transmembrane transport of small molecules. REACT_474. Metabolism of carbohydrates. REACT_6185. HIV Infection.
Gene expression databases
ArrayExpress	Q99567.
Bgee	Q99567.
CleanEx	HS_NUP88.
Genevestigator	Q99567.
GermOnline	ENSG00000108559. Homo sapiens.
Family and domain databases
InterPro	IPR019321. Nucleoporin_Nup88. [Graphical view]
KO	K14318.
Pfam	PF10168. Nup88. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	18975.
SOURCE	Search...

Entry information

Entry name

NUP88_HUMAN

Accession

Primary (citable) accession number: Q99567
Secondary accession number(s): D3DTM2, Q9BWE5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	November 8, 2002
Last modified:	January 25, 2012
This is version 94 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.