UniProtKB - Q99543 (DNJC2_HUMAN)
(max 400 entries)x
Your basket is currently empty. i
Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)
Protein
DnaJ homolog subfamily C member 2
Gene
DNAJC2
Organism
Homo sapiens (Human)
Status
Functioni
Acts both as a chaperone in the cytosol and as a chromatin regulator in the nucleus. When cytosolic, acts as a molecular chaperone: component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, stimulates the ATPase activity of the ribosome-associated pool of Hsp70-type chaperones HSPA14 that bind to the nascent polypeptide chain. When nuclear, mediates the switching from polycomb-repressed genes to an active state: specifically recruited at histone H2A ubiquitinated at 'Lys-119' (H2AK119ub), and promotes the displacement of the polycomb PRC1 complex from chromatin, thereby facilitating transcription activation. Specifically binds DNA sequence 5'-GTCAAGC-3'.3 Publications
Miscellaneous
Constitutes a myeloid leukemia-associated antigen and might be a target for leukemia T-cell therapy.
GO - Molecular functioni
- ATPase activator activity Source: Reactome
- chromatin binding Source: UniProtKB
- DNA binding Source: InterPro
- histone binding Source: UniProtKB
- Hsp70 protein binding Source: UniProtKB
- RNA binding Source: UniProtKB
- RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: NTNU_SB
- ubiquitin modification-dependent histone binding Source: UniProtKB
GO - Biological processi
- 'de novo' cotranslational protein folding Source: UniProtKB
- covalent chromatin modification Source: UniProtKB-KW
- DNA replication Source: Ensembl
- negative regulation of DNA biosynthetic process Source: Ensembl
- positive regulation of transcription, DNA-templated Source: UniProtKB
- regulation of cellular response to heat Source: Reactome
- transcription, DNA-templated Source: UniProtKB-KW
Keywordsi
Molecular function | Activator, Chaperone, Chromatin regulator |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-HSA-3371453. Regulation of HSF1-mediated heat shock response. |
Names & Taxonomyi
Protein namesi | Recommended name: DnaJ homolog subfamily C member 2Alternative name(s): M-phase phosphoprotein 11 Zuotin-related factor 1 Cleaved into the following chain: |
Gene namesi | Name:DNAJC2 Synonyms:MPHOSPH11, MPP11, ZRF1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000105821.14. |
HGNCi | HGNC:13192. DNAJC2. |
MIMi | 605502. gene. |
neXtProti | NX_Q99543. |
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 512 – 513 | HQ → AA: Loss of function. 1 Publication | 2 |
Organism-specific databases
DisGeNETi | 27000. |
OpenTargetsi | ENSG00000105821. |
PharmGKBi | PA162383835. |
Polymorphism and mutation databases
BioMutai | DNAJC2. |
DMDMi | 296439472. |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000425752 | 1 – 621 | DnaJ homolog subfamily C member 2Add BLAST | 621 | |
Initiator methioninei | Removed; alternate1 Publication | |||
ChainiPRO_0000071123 | 2 – 621 | DnaJ homolog subfamily C member 2, N-terminally processedAdd BLAST | 620 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources | 1 | |
Modified residuei | 47 | PhosphoserineCombined sources | 1 | |
Modified residuei | 49 | PhosphoserineCombined sources | 1 | |
Modified residuei | 60 | PhosphoserineCombined sources | 1 | |
Modified residuei | 63 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Phosphorylated in M (mitotic) phase.1 Publication
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q99543. |
MaxQBi | Q99543. |
PaxDbi | Q99543. |
PeptideAtlasi | Q99543. |
PRIDEi | Q99543. |
PTM databases
iPTMneti | Q99543. |
PhosphoSitePlusi | Q99543. |
Expressioni
Tissue specificityi
Widely expressed.1 Publication
Inductioni
Expression is repressed by CEBPA. Strongly overexpressed in leukemic cells.3 Publications
Gene expression databases
Bgeei | ENSG00000105821. |
CleanExi | HS_DNAJC2. |
ExpressionAtlasi | Q99543. baseline and differential. |
Genevisiblei | Q99543. HS. |
Organism-specific databases
HPAi | HPA020454. |
Interactioni
Subunit structurei
Interacts (via ZRF1-UBD region) with ID1 (By similarity). Component of ribosome-associated complex (RAC), a heterodimer composed of Hsp70/DnaK-type chaperone HSPA14 and Hsp40/DnaJ-type chaperone DNAJC2.By similarity1 Publication
Binary interactionsi
With | Entry | #Exp. | IntAct | Notes |
---|---|---|---|---|
HIST1H2AM | P0C0S8 | 2 | EBI-11017224,EBI-1390628 |
GO - Molecular functioni
- histone binding Source: UniProtKB
- Hsp70 protein binding Source: UniProtKB
- ubiquitin modification-dependent histone binding Source: UniProtKB
Protein-protein interaction databases
BioGridi | 117946. 20 interactors. |
DIPi | DIP-60462N. |
IntActi | Q99543. 5 interactors. |
STRINGi | 9606.ENSP00000368565. |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more detailsFeature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Helixi | 556 – 568 | Combined sources | 13 | |
Helixi | 576 – 583 | Combined sources | 8 | |
Helixi | 589 – 618 | Combined sources | 30 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2M2E | NMR | - | A | 551-621 | [»] | |
ProteinModelPortali | Q99543. | |||||
SMRi | Q99543. | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 88 – 161 | JPROSITE-ProRule annotationAdd BLAST | 74 | |
Domaini | 449 – 511 | SANT 1PROSITE-ProRule annotationAdd BLAST | 63 | |
Domaini | 549 – 604 | SANT 2PROSITE-ProRule annotationAdd BLAST | 56 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 23 – 31 | Epitope (recognized by CD8(+) cytotoxic T-lymphocytes) | 9 | |
Regioni | 160 – 250 | ZRF1-UBDAdd BLAST | 91 |
Domaini
The ZRF1-UBD region specifically recognizes and binds H2AK119ub. The ZRF1-UBD region is also involved in protein-protein interactions with other proteins, suggesting that it may be masked by some regulator, thereby preventing its association with H2AK119ub.1 Publication
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0724. Eukaryota. COG5269. LUCA. |
GeneTreei | ENSGT00530000063419. |
HOGENOMi | HOG000006900. |
HOVERGENi | HBG008782. |
InParanoidi | Q99543. |
KOi | K09522. |
OMAi | TTPDRWD. |
OrthoDBi | EOG091G04S8. |
PhylomeDBi | Q99543. |
TreeFami | TF105834. |
Family and domain databases
CDDi | cd06257. DnaJ. 1 hit. cd00167. SANT. 2 hits. |
Gene3Di | 1.10.287.110. 1 hit. |
InterProi | View protein in InterPro IPR001623. DnaJ_domain. IPR018253. DnaJ_domain_CS. IPR009057. Homeobox-like_sf. IPR036869. J_dom_sf. IPR032003. RAC_head. IPR001005. SANT/Myb. IPR017884. SANT_dom. |
Pfami | View protein in Pfam PF00226. DnaJ. 1 hit. PF00249. Myb_DNA-binding. 2 hits. PF16717. RAC_head. 1 hit. |
SMARTi | View protein in SMART SM00271. DnaJ. 1 hit. SM00717. SANT. 2 hits. |
SUPFAMi | SSF46565. SSF46565. 1 hit. SSF46689. SSF46689. 2 hits. |
PROSITEi | View protein in PROSITE PS00636. DNAJ_1. 1 hit. PS50076. DNAJ_2. 1 hit. PS51293. SANT. 2 hits. |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q99543-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
10 20 30 40 50
MLLLPSAADG RGTAITHALT SASTLCQVEP VGRWFEAFVK RRNRNASASF
60 70 80 90 100
QELEDKKELS EESEDEELQL EEFPMLKTLD PKDWKNQDHY AVLGLGHVRY
110 120 130 140 150
KATQRQIKAA HKAMVLKHHP DKRKAAGEPI KEGDNDYFTC ITKAYEMLSD
160 170 180 190 200
PVKRRAFNSV DPTFDNSVPS KSEAKDNFFE VFTPVFERNS RWSNKKNVPK
210 220 230 240 250
LGDMNSSFED VDIFYSFWYN FDSWREFSYL DEEEKEKAEC RDERRWIEKQ
260 270 280 290 300
NRATRAQRKK EEMNRIRTLV DNAYSCDPRI KKFKEEEKAK KEAEKKAKAE
310 320 330 340 350
AKRKEQEAKE KQRQAELEAA RLAKEKEEEE VRQQALLAKK EKDIQKKAIK
360 370 380 390 400
KERQKLRNSC KTWNHFSDNE AERVKMMEEV EKLCDRLELA SLQCLNETLT
410 420 430 440 450
SCTKEVGKAA LEKQIEEINE QIRKEKEEAE ARMRQASKNT EKSTGGGGNG
460 470 480 490 500
SKNWSEDDLQ LLIKAVNLFP AGTNSRWEVI ANYMNIHSSS GVKRTAKDVI
510 520 530 540 550
GKAKSLQKLD PHQKDDINKK AFDKFKKEHG VVPQADNATP SERFEGPYTD
560 570 580 590 600
FTPWTTEEQK LLEQALKTYP VNTPERWEKI AEAVPGRTKK DCMKRYKELV
610 620
EMVKAKKAAQ EQVLNASRAK K
Sequence cautioni
The sequence AAI39752 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAA66913 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 252 | R → G in CAA66913 (PubMed:8885239).Curated | 1 | |
Sequence conflicti | 472 | G → R in CAA66913 (PubMed:8885239).Curated | 1 | |
Sequence conflicti | 578 | E → K in AAI39752 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_023562 | 362 – 414 | Missing in isoform 2. 1 PublicationAdd BLAST | 53 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X98260 mRNA. Translation: CAA66913.1. Different initiation. AC004668 Genomic DNA. No translation available. BC000859 mRNA. Translation: AAH00859.1. BC139751 mRNA. Translation: AAI39752.1. Different initiation. |
CCDSi | CCDS43628.1. [Q99543-1] CCDS47679.1. [Q99543-2] |
RefSeqi | NP_001123359.1. NM_001129887.1. [Q99543-2] NP_055192.1. NM_014377.1. [Q99543-1] |
UniGenei | Hs.558476. |
Genome annotation databases
Ensembli | ENST00000249270; ENSP00000249270; ENSG00000105821. [Q99543-2] ENST00000379263; ENSP00000368565; ENSG00000105821. [Q99543-1] |
GeneIDi | 27000. |
KEGGi | hsa:27000. |
UCSCi | uc003vbo.4. human. [Q99543-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Entry informationi
Entry namei | DNJC2_HUMAN | |
Accessioni | Q99543Primary (citable) accession number: Q99543 Secondary accession number(s): A4VCI0, Q9BVX1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 11, 2001 |
Last sequence update: | May 18, 2010 | |
Last modified: | March 28, 2018 | |
This is version 166 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |