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UniProtKB - Q99543 (DNJC2_HUMAN)

UniProt

Q99543 - DNJC2_HUMAN

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Protein

DnaJ homolog subfamily C member 2

Gene

DNAJC2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts both as a chaperone in the cytosol and as a chromatin regulator in the nucleus. When cytosolic, acts as a molecular chaperone: component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, stimulates the ATPase activity of the ribosome-associated pool of Hsp70-type chaperones HSPA14 that bind to the nascent polypeptide chain. When nuclear, mediates the switching from polycomb-repressed genes to an active state: specifically recruited at histone H2A ubiquitinated at 'Lys-119' (H2AK119ub), and promotes the displacement of the polycomb PRC1 complex from chromatin, thereby facilitating transcription activation. Specifically binds DNA sequence 5'-GTCAAGC-3'.3 Publications

GO - Molecular functioni

  • ATPase activator activity Source: Reactome
  • chromatin binding Source: UniProtKB
  • DNA binding Source: InterPro
  • histone binding Source: UniProtKB
  • Hsp70 protein binding Source: UniProtKB
  • poly(A) RNA binding Source: UniProtKB
  • ubiquitin binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Chaperone, Chromatin regulator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-3371453. Regulation of HSF1-mediated heat shock response.

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ homolog subfamily C member 2
Alternative name(s):
M-phase phosphoprotein 11
Zuotin-related factor 1
Cleaved into the following chain:
DnaJ homolog subfamily C member 2, N-terminally processed
Gene namesi
Name:DNAJC2
Synonyms:MPHOSPH11, MPP11, ZRF1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:13192. DNAJC2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: UniProtKB
  • nuclear membrane Source: HPA
  • nucleoplasm Source: Reactome
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi512 – 5132HQ → AA: Loss of function. 1 Publication

Organism-specific databases

PharmGKBiPA162383835.

Polymorphism and mutation databases

BioMutaiDNAJC2.
DMDMi296439472.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 621621DnaJ homolog subfamily C member 2PRO_0000425752Add
BLAST
Initiator methionineiRemoved; alternate1 Publication
Chaini2 – 621620DnaJ homolog subfamily C member 2, N-terminally processedPRO_0000071123Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineCombined sources
Modified residuei47 – 471PhosphoserineCombined sources
Modified residuei49 – 491PhosphoserineCombined sources
Modified residuei60 – 601PhosphoserineCombined sources
Modified residuei63 – 631PhosphoserineCombined sources

Post-translational modificationi

Phosphorylated in M (mitotic) phase.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ99543.
MaxQBiQ99543.
PaxDbiQ99543.
PeptideAtlasiQ99543.
PRIDEiQ99543.

PTM databases

iPTMnetiQ99543.
PhosphoSiteiQ99543.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Inductioni

Expression is repressed by CEBPA. Strongly overexpressed in leukemic cells.3 Publications

Gene expression databases

BgeeiENSG00000105821.
CleanExiHS_DNAJC2.
ExpressionAtlasiQ99543. baseline and differential.
GenevisibleiQ99543. HS.

Organism-specific databases

HPAiHPA020454.

Interactioni

Subunit structurei

Interacts (via ZRF1-UBD region) with ID1 (By similarity). Component of ribosome-associated complex (RAC), a heterodimer composed of Hsp70/DnaK-type chaperone HSPA14 and Hsp40/DnaJ-type chaperone DNAJC2.By similarity1 Publication

GO - Molecular functioni

  • histone binding Source: UniProtKB
  • Hsp70 protein binding Source: UniProtKB
  • ubiquitin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi117946. 19 interactions.
DIPiDIP-60462N.
IntActiQ99543. 2 interactions.
STRINGi9606.ENSP00000368565.

Structurei

Secondary structure

1
621
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi556 – 56813Combined sources
Helixi576 – 5838Combined sources
Helixi589 – 61830Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2M2ENMR-A551-621[»]
ProteinModelPortaliQ99543.
SMRiQ99543. Positions 87-163, 551-620.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini88 – 16174JPROSITE-ProRule annotationAdd
BLAST
Domaini449 – 51163SANT 1PROSITE-ProRule annotationAdd
BLAST
Domaini549 – 60456SANT 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni23 – 319Epitope (recognized by CD8(+) cytotoxic T-lymphocytes)
Regioni160 – 25091ZRF1-UBDAdd
BLAST

Domaini

The ZRF1-UBD region specifically recognizes and binds H2AK119ub. The ZRF1-UBD region is also involved in protein-protein interactions with other proteins, suggesting that it may be masked by some regulator, thereby preventing its association with H2AK119ub.1 Publication

Sequence similaritiesi

Contains 1 J domain.PROSITE-ProRule annotation
Contains 2 SANT domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0724. Eukaryota.
COG5269. LUCA.
GeneTreeiENSGT00530000063419.
HOGENOMiHOG000006900.
HOVERGENiHBG008782.
InParanoidiQ99543.
KOiK09522.
OMAiLTSCTKE.
OrthoDBiEOG091G04S8.
PhylomeDBiQ99543.
TreeFamiTF105834.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.10.60. 1 hit.
1.10.287.110. 1 hit.
InterProiIPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR009057. Homeodomain-like.
IPR032003. RAC_head.
IPR001005. SANT/Myb.
IPR017884. SANT_dom.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF00249. Myb_DNA-binding. 2 hits.
PF16717. RAC_head. 1 hit.
[Graphical view]
SMARTiSM00271. DnaJ. 1 hit.
SM00717. SANT. 2 hits.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF46689. SSF46689. 2 hits.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51293. SANT. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q99543-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLLLPSAADG RGTAITHALT SASTLCQVEP VGRWFEAFVK RRNRNASASF 
        60         70         80         90        100
QELEDKKELS EESEDEELQL EEFPMLKTLD PKDWKNQDHY AVLGLGHVRY 
       110        120        130        140        150
KATQRQIKAA HKAMVLKHHP DKRKAAGEPI KEGDNDYFTC ITKAYEMLSD 
       160        170        180        190        200
PVKRRAFNSV DPTFDNSVPS KSEAKDNFFE VFTPVFERNS RWSNKKNVPK 
       210        220        230        240        250
LGDMNSSFED VDIFYSFWYN FDSWREFSYL DEEEKEKAEC RDERRWIEKQ 
       260        270        280        290        300
NRATRAQRKK EEMNRIRTLV DNAYSCDPRI KKFKEEEKAK KEAEKKAKAE 
       310        320        330        340        350
AKRKEQEAKE KQRQAELEAA RLAKEKEEEE VRQQALLAKK EKDIQKKAIK 
       360        370        380        390        400
KERQKLRNSC KTWNHFSDNE AERVKMMEEV EKLCDRLELA SLQCLNETLT 
       410        420        430        440        450
SCTKEVGKAA LEKQIEEINE QIRKEKEEAE ARMRQASKNT EKSTGGGGNG 
       460        470        480        490        500
SKNWSEDDLQ LLIKAVNLFP AGTNSRWEVI ANYMNIHSSS GVKRTAKDVI 
       510        520        530        540        550
GKAKSLQKLD PHQKDDINKK AFDKFKKEHG VVPQADNATP SERFEGPYTD 
       560        570        580        590        600
FTPWTTEEQK LLEQALKTYP VNTPERWEKI AEAVPGRTKK DCMKRYKELV 
       610        620 
EMVKAKKAAQ EQVLNASRAK K
Length:621
Mass (Da):71,996
Last modified:May 18, 2010 - v4
Checksum:iE4DAEE7A73D0F64C
GO
Isoform 2 (identifier: Q99543-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     362-414: Missing.

Show »
Length:568
Mass (Da):65,913
Checksum:i390BA8AC8C0DFCAD
GO

Sequence cautioni

The sequence AAI39752 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAA66913 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti252 – 2521R → G in CAA66913 (PubMed:8885239).Curated
Sequence conflicti472 – 4721G → R in CAA66913 (PubMed:8885239).Curated
Sequence conflicti578 – 5781E → K in AAI39752 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei362 – 41453Missing in isoform 2. 1 PublicationVSP_023562Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98260 mRNA. Translation: CAA66913.1. Different initiation.
AC004668 Genomic DNA. No translation available.
BC000859 mRNA. Translation: AAH00859.1.
BC139751 mRNA. Translation: AAI39752.1. Different initiation.
CCDSiCCDS43628.1. [Q99543-1]
CCDS47679.1. [Q99543-2]
RefSeqiNP_001123359.1. NM_001129887.1. [Q99543-2]
NP_055192.1. NM_014377.1. [Q99543-1]
UniGeneiHs.558476.

Genome annotation databases

EnsembliENST00000249270; ENSP00000249270; ENSG00000105821. [Q99543-2]
ENST00000379263; ENSP00000368565; ENSG00000105821. [Q99543-1]
GeneIDi27000.
KEGGihsa:27000.
UCSCiuc003vbo.4. human. [Q99543-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98260 mRNA. Translation: CAA66913.1. Different initiation.
AC004668 Genomic DNA. No translation available.
BC000859 mRNA. Translation: AAH00859.1.
BC139751 mRNA. Translation: AAI39752.1. Different initiation.
CCDSiCCDS43628.1. [Q99543-1]
CCDS47679.1. [Q99543-2]
RefSeqiNP_001123359.1. NM_001129887.1. [Q99543-2]
NP_055192.1. NM_014377.1. [Q99543-1]
UniGeneiHs.558476.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2M2ENMR-A551-621[»]
ProteinModelPortaliQ99543.
SMRiQ99543. Positions 87-163, 551-620.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117946. 19 interactions.
DIPiDIP-60462N.
IntActiQ99543. 2 interactions.
STRINGi9606.ENSP00000368565.

PTM databases

iPTMnetiQ99543.
PhosphoSiteiQ99543.

Polymorphism and mutation databases

BioMutaiDNAJC2.
DMDMi296439472.

Proteomic databases

EPDiQ99543.
MaxQBiQ99543.
PaxDbiQ99543.
PeptideAtlasiQ99543.
PRIDEiQ99543.

Protocols and materials databases

DNASUi27000.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000249270; ENSP00000249270; ENSG00000105821. [Q99543-2]
ENST00000379263; ENSP00000368565; ENSG00000105821. [Q99543-1]
GeneIDi27000.
KEGGihsa:27000.
UCSCiuc003vbo.4. human. [Q99543-1]

Organism-specific databases

CTDi27000.
GeneCardsiDNAJC2.
HGNCiHGNC:13192. DNAJC2.
HPAiHPA020454.
MIMi605502. gene.
neXtProtiNX_Q99543.
PharmGKBiPA162383835.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0724. Eukaryota.
COG5269. LUCA.
GeneTreeiENSGT00530000063419.
HOGENOMiHOG000006900.
HOVERGENiHBG008782.
InParanoidiQ99543.
KOiK09522.
OMAiLTSCTKE.
OrthoDBiEOG091G04S8.
PhylomeDBiQ99543.
TreeFamiTF105834.

Enzyme and pathway databases

ReactomeiR-HSA-3371453. Regulation of HSF1-mediated heat shock response.

Miscellaneous databases

GeneWikiiZRF1.
GenomeRNAii27000.
PROiQ99543.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000105821.
CleanExiHS_DNAJC2.
ExpressionAtlasiQ99543. baseline and differential.
GenevisibleiQ99543. HS.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.10.60. 1 hit.
1.10.287.110. 1 hit.
InterProiIPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR009057. Homeodomain-like.
IPR032003. RAC_head.
IPR001005. SANT/Myb.
IPR017884. SANT_dom.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF00249. Myb_DNA-binding. 2 hits.
PF16717. RAC_head. 1 hit.
[Graphical view]
SMARTiSM00271. DnaJ. 1 hit.
SM00717. SANT. 2 hits.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF46689. SSF46689. 2 hits.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51293. SANT. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDNJC2_HUMAN
AccessioniPrimary (citable) accession number: Q99543
Secondary accession number(s): A4VCI0, Q9BVX1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: May 18, 2010
Last modified: September 7, 2016
This is version 151 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Constitutes a myeloid leukemia-associated antigen and might be a target for leukemia T-cell therapy.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.