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Protein

Synaptic vesicle membrane protein VAT-1 homolog

Gene

VAT1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Possesses ATPase activity (By similarity). Plays a part in calcium-regulated keratinocyte activation in epidermal repair mechanisms. Has no effect on cell proliferation. Negatively regulates mitochondrial fusion in cooperation with mitofusin proteins (MFN1-2).By similarity3 Publications

GO - Molecular functioni

GO - Biological processi

  • negative regulation of mitochondrial fusion Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptic vesicle membrane protein VAT-1 homolog (EC:1.-.-.-)
Gene namesi
Name:VAT1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:16919. VAT1.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
  • integral component of membrane Source: ProtInc
  • mitochondrial outer membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA38422.

Polymorphism and mutation databases

BioMutaiVAT1.
DMDMi52788294.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources1 Publication
Chaini2 – 393392Synaptic vesicle membrane protein VAT-1 homologPRO_0000160918Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineCombined sources1 Publication
Modified residuei2 – 21PhosphoserineBy similarity
Modified residuei18 – 181PhosphoserineCombined sources
Modified residuei27 – 271PhosphoserineCombined sources
Modified residuei35 – 351PhosphoserineCombined sources
Modified residuei44 – 441PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ99536.
MaxQBiQ99536.
PaxDbiQ99536.
PeptideAtlasiQ99536.
PRIDEiQ99536.
TopDownProteomicsiQ99536-1. [Q99536-1]

PTM databases

iPTMnetiQ99536.
PhosphoSiteiQ99536.
SwissPalmiQ99536.

Expressioni

Tissue specificityi

Expressed in brain. Also expressed in glioblastoma cells.1 Publication

Inductioni

Increased expression in glioblastomas and on wounding, in basal keratinocytes. This expression is calcium ion-dependent.1 Publication

Gene expression databases

BgeeiENSG00000108828.
CleanExiHS_VAT1.
ExpressionAtlasiQ99536. baseline and differential.
GenevisibleiQ99536. HS.

Organism-specific databases

HPAiHPA045170.

Interactioni

Protein-protein interaction databases

BioGridi115756. 20 interactions.
IntActiQ99536. 8 interactions.
MINTiMINT-4999872.
STRINGi9606.ENSP00000347872.

Structurei

3D structure databases

ProteinModelPortaliQ99536.
SMRiQ99536. Positions 37-387.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1198. Eukaryota.
COG0604. LUCA.
GeneTreeiENSGT00550000074483.
HOGENOMiHOG000294681.
HOVERGENiHBG002466.
InParanoidiQ99536.
OMAiFGLWQEV.
OrthoDBiEOG091G0EEJ.
PhylomeDBiQ99536.
TreeFamiTF314255.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR013149. ADH_C.
IPR013154. ADH_N.
IPR002085. ADH_SF_Zn-type.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
IPR020843. PKS_ER.
IPR002364. Quin_OxRdtase/zeta-crystal_CS.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SMARTiSM00829. PKS_ER. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 1 hit.
SSF51735. SSF51735. 1 hit.
PROSITEiPS01162. QOR_ZETA_CRYSTAL. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q99536-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSDEREVAEA ATGEDASSPP PKTEAASDPQ HPAASEGAAA AAASPPLLRC
60 70 80 90 100
LVLTGFGGYD KVKLQSRPAA PPAPGPGQLT LRLRACGLNF ADLMARQGLY
110 120 130 140 150
DRLPPLPVTP GMEGAGVVIA VGEGVSDRKA GDRVMVLNRS GMWQEEVTVP
160 170 180 190 200
SVQTFLIPEA MTFEEAAALL VNYITAYMVL FDFGNLQPGH SVLVHMAAGG
210 220 230 240 250
VGMAAVQLCR TVENVTVFGT ASASKHEALK ENGVTHPIDY HTTDYVDEIK
260 270 280 290 300
KISPKGVDIV MDPLGGSDTA KGYNLLKPMG KVVTYGMANL LTGPKRNLMA
310 320 330 340 350
LARTWWNQFS VTALQLLQAN RAVCGFHLGY LDGEVELVSG VVARLLALYN
360 370 380 390
QGHIKPHIDS VWPFEKVADA MKQMQEKKNV GKVLLVPGPE KEN
Length:393
Mass (Da):41,920
Last modified:September 27, 2004 - v2
Checksum:i25933347742C2FE6
GO
Isoform 2 (identifier: Q99536-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-134: Missing.

Note: No experimental confirmation available.
Show »
Length:259
Mass (Da):28,341
Checksum:iF18FE952D93B9481
GO
Isoform 3 (identifier: Q99536-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     61-128: Missing.

Note: No experimental confirmation available.
Show »
Length:325
Mass (Da):34,890
Checksum:iE7343C6107A69186
GO

Sequence cautioni

The sequence AAC37596 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti71 – 711P → A in AAC37596 (PubMed:8938427).Curated
Sequence conflicti82 – 865RLRAC → NSARG in AAA95990 (PubMed:7774926).Curated
Sequence conflicti207 – 2082QL → HV in AAA95990 (PubMed:7774926).Curated
Sequence conflicti392 – 3921E → Q in AAC37596 (PubMed:8938427).Curated
Sequence conflicti393 – 3931N → S in AAA95990 (PubMed:7774926).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 134134Missing in isoform 2. 1 PublicationVSP_055794Add
BLAST
Alternative sequencei61 – 12868Missing in isoform 3. 1 PublicationVSP_055795Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L78833 Genomic DNA. Translation: AAC37596.1. Different initiation.
BT007369 mRNA. Translation: AAP36033.1.
AK298536 mRNA. Translation: BAG60736.1.
AK290460 mRNA. Translation: BAF83149.1.
AK315838 mRNA. Translation: BAF98729.1.
AC055866 Genomic DNA. No translation available.
AC135721 Genomic DNA. No translation available.
CH471152 Genomic DNA. Translation: EAW60917.1.
BC001913 mRNA. Translation: AAH01913.2.
BC008725 mRNA. Translation: AAH08725.2.
BC014279 mRNA. Translation: AAH14279.1.
BC015041 mRNA. Translation: AAH15041.1.
BC090858 mRNA. Translation: AAH90858.1.
U18009 mRNA. Translation: AAA95990.1.
CCDSiCCDS11451.1. [Q99536-1]
RefSeqiNP_006364.2. NM_006373.3. [Q99536-1]
UniGeneiHs.514199.

Genome annotation databases

EnsembliENST00000355653; ENSP00000347872; ENSG00000108828. [Q99536-1]
ENST00000420567; ENSP00000408553; ENSG00000108828. [Q99536-2]
ENST00000587173; ENSP00000465946; ENSG00000108828. [Q99536-3]
GeneIDi10493.
KEGGihsa:10493.
UCSCiuc002icm.2. human. [Q99536-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L78833 Genomic DNA. Translation: AAC37596.1. Different initiation.
BT007369 mRNA. Translation: AAP36033.1.
AK298536 mRNA. Translation: BAG60736.1.
AK290460 mRNA. Translation: BAF83149.1.
AK315838 mRNA. Translation: BAF98729.1.
AC055866 Genomic DNA. No translation available.
AC135721 Genomic DNA. No translation available.
CH471152 Genomic DNA. Translation: EAW60917.1.
BC001913 mRNA. Translation: AAH01913.2.
BC008725 mRNA. Translation: AAH08725.2.
BC014279 mRNA. Translation: AAH14279.1.
BC015041 mRNA. Translation: AAH15041.1.
BC090858 mRNA. Translation: AAH90858.1.
U18009 mRNA. Translation: AAA95990.1.
CCDSiCCDS11451.1. [Q99536-1]
RefSeqiNP_006364.2. NM_006373.3. [Q99536-1]
UniGeneiHs.514199.

3D structure databases

ProteinModelPortaliQ99536.
SMRiQ99536. Positions 37-387.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115756. 20 interactions.
IntActiQ99536. 8 interactions.
MINTiMINT-4999872.
STRINGi9606.ENSP00000347872.

PTM databases

iPTMnetiQ99536.
PhosphoSiteiQ99536.
SwissPalmiQ99536.

Polymorphism and mutation databases

BioMutaiVAT1.
DMDMi52788294.

Proteomic databases

EPDiQ99536.
MaxQBiQ99536.
PaxDbiQ99536.
PeptideAtlasiQ99536.
PRIDEiQ99536.
TopDownProteomicsiQ99536-1. [Q99536-1]

Protocols and materials databases

DNASUi10493.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000355653; ENSP00000347872; ENSG00000108828. [Q99536-1]
ENST00000420567; ENSP00000408553; ENSG00000108828. [Q99536-2]
ENST00000587173; ENSP00000465946; ENSG00000108828. [Q99536-3]
GeneIDi10493.
KEGGihsa:10493.
UCSCiuc002icm.2. human. [Q99536-1]

Organism-specific databases

CTDi10493.
GeneCardsiVAT1.
HGNCiHGNC:16919. VAT1.
HPAiHPA045170.
MIMi604631. gene.
neXtProtiNX_Q99536.
PharmGKBiPA38422.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1198. Eukaryota.
COG0604. LUCA.
GeneTreeiENSGT00550000074483.
HOGENOMiHOG000294681.
HOVERGENiHBG002466.
InParanoidiQ99536.
OMAiFGLWQEV.
OrthoDBiEOG091G0EEJ.
PhylomeDBiQ99536.
TreeFamiTF314255.

Miscellaneous databases

ChiTaRSiVAT1. human.
GeneWikiiVAT1.
GenomeRNAii10493.
PROiQ99536.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000108828.
CleanExiHS_VAT1.
ExpressionAtlasiQ99536. baseline and differential.
GenevisibleiQ99536. HS.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR013149. ADH_C.
IPR013154. ADH_N.
IPR002085. ADH_SF_Zn-type.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
IPR020843. PKS_ER.
IPR002364. Quin_OxRdtase/zeta-crystal_CS.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SMARTiSM00829. PKS_ER. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 1 hit.
SSF51735. SSF51735. 1 hit.
PROSITEiPS01162. QOR_ZETA_CRYSTAL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVAT1_HUMAN
AccessioniPrimary (citable) accession number: Q99536
Secondary accession number(s): A8K345
, B0AZP7, B4DPX4, Q13035, Q5BKZ7, Q96A39, Q9BUT8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: September 27, 2004
Last modified: September 7, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.