Q99536 (VAT1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Synaptic vesicle membrane protein VAT-1 homolog
EC=1.-.-.-

Gene names

Name:

VAT1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Possesses ATPase activity By similarity. Plays a part in calcium-regulated keratinocyte activation in epidermal repair mechanisms. Has no effect on cell proliferation. Ref.8 Ref.9
Subcellular location	Cytoplasm Ref.8 Ref.9.
Tissue specificity	Expressed in brain. Also expressed in glioblastoma cells. Ref.9
Induction	Increased expression in glioblastomas and on wounding, in basal keratinocytes. This expression is calcium ion-dependent. Ref.9
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.
Sequence caution	The sequence AAC37596.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Traceable author statement. Source: ProtInc
Molecular function	nucleotide binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.6
	Chain	2 – 393	392	Synaptic vesicle membrane protein VAT-1 homolog		PRO_0000160918
Amino acid modifications
	Modified residue	2	1	N-acetylserine Ref.6
Experimental info
	Sequence conflict	71	1	P → A in AAC37596. Ref.1
	Sequence conflict	82 – 86	5	RLRAC → NSARG in AAA95990. Ref.7
	Sequence conflict	207 – 208	2	QL → HV in AAA95990. Ref.7
	Sequence conflict	392	1	E → Q in AAC37596. Ref.1
	Sequence conflict	393	1	N → S in AAA95990. Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Q99536 [UniParc]. Last modified September 27, 2004. Version 2. Checksum: 25933347742C2FE6	FASTA	393	41,920
	10 20 30 40 50 60 MSDEREVAEA ATGEDASSPP PKTEAASDPQ HPAASEGAAA AAASPPLLRC LVLTGFGGYD 70 80 90 100 110 120 KVKLQSRPAA PPAPGPGQLT LRLRACGLNF ADLMARQGLY DRLPPLPVTP GMEGAGVVIA 130 140 150 160 170 180 VGEGVSDRKA GDRVMVLNRS GMWQEEVTVP SVQTFLIPEA MTFEEAAALL VNYITAYMVL 190 200 210 220 230 240 FDFGNLQPGH SVLVHMAAGG VGMAAVQLCR TVENVTVFGT ASASKHEALK ENGVTHPIDY 250 260 270 280 290 300 HTTDYVDEIK KISPKGVDIV MDPLGGSDTA KGYNLLKPMG KVVTYGMANL LTGPKRNLMA 310 320 330 340 350 360 LARTWWNQFS VTALQLLQAN RAVCGFHLGY LDGEVELVSG VVARLLALYN QGHIKPHIDS 370 380 390 VWPFEKVADA MKQMQEKKNV GKVLLVPGPE KEN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genomic sequence and analysis of 117 kb of human DNA containing the gene BRCA1." Smith T.M., Lee M.K., Szabo C.I., Jerome N., McEuen M., Taylor M., Hood L., King M.-C. Genome Res. 6:1029-1049(1996) [PubMed: 8938427] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain, Ovary, Skin and Uterus.
[6]	Bienvenut W.V. Submitted (JAN-2010) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-61; 64-82; 85-96; 103-128; 211-225; 256-271; 282-295 AND 322-344, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2, MASS SPECTROMETRY. Tissue: Ovarian carcinoma.
[7]	"22 genes from chromosome 17q21: cloning, sequencing, and characterization of mutations in breast cancer families and tumors." Friedman L.S., Ostermeyer E.A., Lynch E.D., Szabo C.I., Meza J.E., Anderson L.A., Dowd P., Lee M.K., Rowell S.E., Ellison J., Boyd J., King M.-C. Genomics 25:256-263(1995) [PubMed: 7774926] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 82-393.
[8]	"Human VAT-1: a calcium-regulated activation marker of human epithelial cells." Koch J., Foekens J., Timmermans M., Fink W., Wirzbach A., Kramer M.D., Schaefer B.M. Arch. Dermatol. Res. 295:203-210(2003) [PubMed: 12898150] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[9]	"Vesicle amine transport protein-1 (VAT-1) is upregulated in glioblastomas and promotes migration." Mertsch S., Becker M., Lichota A., Paulus W., Senner V. Neuropathol. Appl. Neurobiol. 35:342-352(2009) [PubMed: 19508442] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION BY WOUNDING.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L78833 Genomic DNA. Translation: AAC37596.1. Different initiation. BT007369 mRNA. Translation: AAP36033.1. AK290460 mRNA. Translation: BAF83149.1. CH471152 Genomic DNA. Translation: EAW60917.1. BC001913 mRNA. Translation: AAH01913.2. BC008725 mRNA. Translation: AAH08725.2. BC014279 mRNA. Translation: AAH14279.1. BC015041 mRNA. Translation: AAH15041.1. BC090858 mRNA. Translation: AAH90858.1. U18009 mRNA. Translation: AAA95990.1.
IPI	IPI00156689.
RefSeq	NP_006364.2. NM_006373.3.
UniGene	Hs.514199.
3D structure databases
ProteinModelPortal	Q99536.
SMR	Q99536. Positions 46-387.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q99536. 2 interactions.
STRING	Q99536.
PTM databases
PhosphoSite	Q99536.
Polymorphism databases
DMDM	52788294.
Proteomic databases
PeptideAtlas	Q99536.
PRIDE	Q99536.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000355653; ENSP00000347872; ENSG00000108828.
GeneID	10493.
KEGG	hsa:10493.
UCSC	uc002icm.1. human.
Organism-specific databases
CTD	10493.
GeneCards	GC17M041166.
H-InvDB	HIX0014088.
HGNC	HGNC:16919. VAT1.
HPA	HPA045170.
MIM	604631. gene.
neXtProt	NX_Q99536.
PharmGKB	PA38422.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG05036.
GeneTree	ENSGT00550000074483.
HOGENOM	HBG753318.
HOVERGEN	HBG002466.
InParanoid	Q99536.
OMA	LPVTPGM.
OrthoDB	EOG4FXR7X.
PhylomeDB	Q99536.
Gene expression databases
ArrayExpress	Q99536.
Bgee	Q99536.
CleanEx	HS_VAT1.
Genevestigator	Q99536.
GermOnline	ENSG00000108828. Homo sapiens.
Family and domain databases
InterPro	IPR013149. ADH_C. IPR013154. ADH_GroES-like. IPR002085. ADH_SF_Zn-type. IPR011032. GroES-like. IPR016040. NAD(P)-bd_dom. IPR002364. Quin_OxRdtase/zeta-crystal_CS. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00540.
PANTHER	PTHR11695. ADH_Sf_Zn. 1 hit.
Pfam	PF08240. ADH_N. 1 hit. PF00107. ADH_zinc_N. 1 hit. [Graphical view]
SUPFAM	SSF50129. GroES_like. 1 hit.
PROSITE	PS01162. QOR_ZETA_CRYSTAL. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	39818.
SOURCE	Search...

Entry information

Entry name

VAT1_HUMAN

Accession

Primary (citable) accession number: Q99536
Secondary accession number(s): A8K345 , Q13035, Q5BKZ7, Q96A39, Q9BUT8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	September 27, 2004
Last modified:	January 25, 2012
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.