Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Dimethylaniline monooxygenase [N-oxide-forming] 2

Gene

FMO2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the N-oxidation of certain primary alkylamines to their oximes via an N-hydroxylamine intermediate. Inactive toward certain tertiary amines, such as imipramine or chloropromazine. Can catalyze the S-oxidation of methimazole. The truncated form is catalytically inactive.1 Publication

Catalytic activityi

N,N-dimethylaniline + NADPH + O2 = N,N-dimethylaniline N-oxide + NADP+ + H2O.

Cofactori

Protein has several cofactor binding sites:

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi9 – 14FADSequence analysis6
Nucleotide bindingi191 – 196NADPSequence analysis6

GO - Molecular functioni

GO - Biological processi

  • drug metabolic process Source: BHF-UCL
  • NADPH oxidation Source: UniProtKB
  • NADP metabolic process Source: BHF-UCL
  • organic acid metabolic process Source: BHF-UCL
  • oxygen metabolic process Source: Ensembl
  • toxin metabolic process Source: BHF-UCL
  • xenobiotic metabolic process Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, Magnesium, NADP

Enzyme and pathway databases

BioCyciZFISH:HS01807-MONOMER.
BRENDAi1.14.13.8. 2681.
ReactomeiR-HSA-217271. FMO oxidises nucleophiles.

Names & Taxonomyi

Protein namesi
Recommended name:
Dimethylaniline monooxygenase [N-oxide-forming] 2 (EC:1.14.13.8)
Alternative name(s):
Dimethylaniline oxidase 2
FMO 1B1
Pulmonary flavin-containing monooxygenase 2
Short name:
FMO 2
Gene namesi
Name:FMO2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:3770. FMO2.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: Reactome
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: UniProtKB
  • organelle membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Organism-specific databases

DisGeNETi2327.
PharmGKBiPA164741534.

Chemistry databases

ChEMBLiCHEMBL3542432.

Polymorphism and mutation databases

DMDMi327478599.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001476462 – 471Dimethylaniline monooxygenase [N-oxide-forming] 2Add BLAST470

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1

Post-translational modificationi

The truncated form is probably unable to fold correctly and is rapidly degraded.
FMO2*1 is sumoylated at 'Lys-492'.1 Publication

Keywords - PTMi

Acetylation, Ubl conjugation

Proteomic databases

PaxDbiQ99518.
PeptideAtlasiQ99518.
PRIDEiQ99518.

PTM databases

iPTMnetiQ99518.
PhosphoSitePlusiQ99518.

Expressioni

Tissue specificityi

Expressed in lung (at protein level). Expressed predominantly in lung, and at a much lesser extent in kidney. Also expressed in fetal lung, but not in liver, kidney and brain.2 Publications

Gene expression databases

BgeeiENSG00000094963.
CleanExiHS_FMO2.
ExpressionAtlasiQ99518. baseline and differential.
GenevisibleiQ99518. HS.

Organism-specific databases

HPAiHPA028261.

Interactioni

Protein-protein interaction databases

BioGridi108614. 1 interactor.
IntActiQ99518. 2 interactors.
STRINGi9606.ENSP00000209929.

Structurei

3D structure databases

ProteinModelPortaliQ99518.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the FMO family.Curated

Keywords - Domaini

Transmembrane

Phylogenomic databases

eggNOGiKOG1399. Eukaryota.
COG2072. LUCA.
HOGENOMiHOG000076537.
HOVERGENiHBG002037.
InParanoidiQ99518.
KOiK00485.
OrthoDBiEOG091G0465.
PhylomeDBiQ99518.
TreeFamiTF105285.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
InterProiIPR012143. DiMe-aniline_mOase.
IPR023753. FAD/NAD-binding_dom.
IPR000960. Flavin_mOase.
IPR020946. Flavin_mOase-like.
IPR002254. Flavin_mOase_2.
[Graphical view]
PfamiPF00743. FMO-like. 1 hit.
[Graphical view]
PIRSFiPIRSF000332. FMO. 1 hit.
PRINTSiPR00370. FMOXYGENASE.
PR01122. FMOXYGENASE2.
SUPFAMiSSF51905. SSF51905. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99518-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKKVAVIGA GVSGLISLKC CVDEGLEPTC FERTEDIGGV WRFKENVEDG
60 70 80 90 100
RASIYQSVVT NTSKEMSCFS DFPMPEDFPN FLHNSKLLEY FRIFAKKFDL
110 120 130 140 150
LKYIQFQTTV LSVRKCPDFS SSGQWKVVTQ SNGKEQSAVF DAVMVCSGHH
160 170 180 190 200
ILPHIPLKSF PGMERFKGQY FHSRQYKHPD GFEGKRILVI GMGNSGSDIA
210 220 230 240 250
VELSKNAAQV FISTRHGTWV MSRISEDGYP WDSVFHTRFR SMLRNVLPRT
260 270 280 290 300
AVKWMIEQQM NRWFNHENYG LEPQNKYIMK EPVLNDDVPS RLLCGAIKVK
310 320 330 340 350
STVKELTETS AIFEDGTVEE NIDVIIFATG YSFSFPFLED SLVKVENNMV
360 370 380 390 400
SLYKYIFPAH LDKSTLACIG LIQPLGSIFP TAELQARWVT RVFKGLCSLP
410 420 430 440 450
SERTMMMDII KRNEKRIDLF GESQSQTLQT NYVDYLDELA LEIGAKPDFC
460 470
SLLFKDPKLA VRLYFGPCNS Y
Length:471
Mass (Da):53,644
Last modified:April 5, 2011 - v4
Checksum:iAF87E304131BA703
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti71D → DD in AAW82431 (Ref. 2) Curated1

Polymorphismi

The sequence shown is that of the allele FMO2*2A. There are two alleles; one major, FMO2*2A (truncated form) and one minor, FMO2*1 (full-length form similar to the protein found in other mammals) (PubMed:9804831). A nonsense mutation transforms the Gln-472 of FMO2*1 in a premature stop codon (PubMed:9804831). FMO2*2A occurs in essentially 100% of Caucasians and Asians (PubMed:9804831). FMO2*1 is present at a frequency of approximately 4% to 13% in the sample of population of African descent (PubMed:9804831, PubMed:11042094, PubMed:18794725). FMO2*2A is catalytically inactive (PubMed:9804831).3 Publications

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01484036D → G.2 PublicationsCorresponds to variant rs2020870dbSNPEnsembl.1
Natural variantiVAR_01536159V → I.1 PublicationCorresponds to variant rs55708639dbSNPEnsembl.1
Natural variantiVAR_02218569F → Y.1 PublicationCorresponds to variant rs28745274dbSNPEnsembl.1
Natural variantiVAR_01484181F → S.1 PublicationCorresponds to variant rs2020860dbSNPEnsembl.1
Natural variantiVAR_014842182F → S.2 PublicationsCorresponds to variant rs2307492dbSNPEnsembl.1
Natural variantiVAR_014843195S → L.2 PublicationsCorresponds to variant rs2020862dbSNPEnsembl.1
Natural variantiVAR_015362238R → Q.2 PublicationsCorresponds to variant rs28369895dbSNPEnsembl.1
Natural variantiVAR_022186314E → G.1 PublicationCorresponds to variant rs2020863dbSNPEnsembl.1
Natural variantiVAR_015363391R → T.2 PublicationsCorresponds to variant rs28369899dbSNPEnsembl.1
Natural variantiVAR_014844413N → K.2 PublicationsCorresponds to variant rs2020865dbSNPEnsembl.1
Natural variantiVAR_064887471Y → YQYRLVGPGQWEGARNAIFT QKQRILKPLKTRALKDSSNF SVSFLLKILGLLAVVVAFFC QLQWS in allele FMO2*1. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09267 mRNA. Translation: CAA70462.1.
AY916056 Genomic DNA. Translation: AAW82431.1.
BT006979 mRNA. Translation: AAP35625.1.
AL021026 Genomic DNA. Translation: CAA15910.1.
CH471067 Genomic DNA. Translation: EAW90889.1.
BC005894 mRNA. Translation: AAH05894.1.
RefSeqiNP_001451.2. NM_001460.4.
UniGeneiHs.144912.

Genome annotation databases

EnsembliENST00000209929; ENSP00000209929; ENSG00000094963.
GeneIDi2327.
KEGGihsa:2327.
UCSCiuc057ngi.1. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09267 mRNA. Translation: CAA70462.1.
AY916056 Genomic DNA. Translation: AAW82431.1.
BT006979 mRNA. Translation: AAP35625.1.
AL021026 Genomic DNA. Translation: CAA15910.1.
CH471067 Genomic DNA. Translation: EAW90889.1.
BC005894 mRNA. Translation: AAH05894.1.
RefSeqiNP_001451.2. NM_001460.4.
UniGeneiHs.144912.

3D structure databases

ProteinModelPortaliQ99518.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108614. 1 interactor.
IntActiQ99518. 2 interactors.
STRINGi9606.ENSP00000209929.

Chemistry databases

ChEMBLiCHEMBL3542432.

PTM databases

iPTMnetiQ99518.
PhosphoSitePlusiQ99518.

Polymorphism and mutation databases

DMDMi327478599.

Proteomic databases

PaxDbiQ99518.
PeptideAtlasiQ99518.
PRIDEiQ99518.

Protocols and materials databases

DNASUi2327.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000209929; ENSP00000209929; ENSG00000094963.
GeneIDi2327.
KEGGihsa:2327.
UCSCiuc057ngi.1. human.

Organism-specific databases

CTDi2327.
DisGeNETi2327.
GeneCardsiFMO2.
HGNCiHGNC:3770. FMO2.
HPAiHPA028261.
MIMi603955. gene.
neXtProtiNX_Q99518.
PharmGKBiPA164741534.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1399. Eukaryota.
COG2072. LUCA.
HOGENOMiHOG000076537.
HOVERGENiHBG002037.
InParanoidiQ99518.
KOiK00485.
OrthoDBiEOG091G0465.
PhylomeDBiQ99518.
TreeFamiTF105285.

Enzyme and pathway databases

BioCyciZFISH:HS01807-MONOMER.
BRENDAi1.14.13.8. 2681.
ReactomeiR-HSA-217271. FMO oxidises nucleophiles.

Miscellaneous databases

ChiTaRSiFMO2. human.
GeneWikiiFMO2.
GenomeRNAii2327.
PROiQ99518.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000094963.
CleanExiHS_FMO2.
ExpressionAtlasiQ99518. baseline and differential.
GenevisibleiQ99518. HS.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
InterProiIPR012143. DiMe-aniline_mOase.
IPR023753. FAD/NAD-binding_dom.
IPR000960. Flavin_mOase.
IPR020946. Flavin_mOase-like.
IPR002254. Flavin_mOase_2.
[Graphical view]
PfamiPF00743. FMO-like. 1 hit.
[Graphical view]
PIRSFiPIRSF000332. FMO. 1 hit.
PRINTSiPR00370. FMOXYGENASE.
PR01122. FMOXYGENASE2.
SUPFAMiSSF51905. SSF51905. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiFMO2_HUMAN
AccessioniPrimary (citable) accession number: Q99518
Secondary accession number(s): Q5EBX4, Q86U73, Q9BRX1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: April 5, 2011
Last modified: November 2, 2016
This is version 162 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.