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Protein

Dimethylaniline monooxygenase [N-oxide-forming] 2

Gene

FMO2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the N-oxidation of certain primary alkylamines to their oximes via an N-hydroxylamine intermediate. Inactive toward certain tertiary amines, such as imipramine or chloropromazine. Can catalyze the S-oxidation of methimazole. The truncated form is catalytically inactive.1 Publication

Catalytic activityi

N,N-dimethylaniline + NADPH + O2 = N,N-dimethylaniline N-oxide + NADP+ + H2O.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei32FADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi9 – 13FADBy similarity5
Nucleotide bindingi40 – 41FADBy similarity2
Nucleotide bindingi60 – 61NADPBy similarity2
Nucleotide bindingi61 – 62FADBy similarity2
Nucleotide bindingi195 – 198NADPBy similarity4

GO - Molecular functioni

GO - Biological processi

  • drug metabolic process Source: BHF-UCL
  • NADPH oxidation Source: UniProtKB
  • NADP metabolic process Source: BHF-UCL
  • organic acid metabolic process Source: BHF-UCL
  • oxygen metabolic process Source: Ensembl
  • toxin metabolic process Source: BHF-UCL
  • xenobiotic metabolic process Source: BHF-UCL

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
LigandFAD, Flavoprotein, Magnesium, NADP

Enzyme and pathway databases

BRENDAi1.14.13.8 2681
ReactomeiR-HSA-217271 FMO oxidises nucleophiles

Names & Taxonomyi

Protein namesi
Recommended name:
Dimethylaniline monooxygenase [N-oxide-forming] 2 (EC:1.14.13.8)
Alternative name(s):
Dimethylaniline oxidase 2
FMO 1B1
Pulmonary flavin-containing monooxygenase 2
Short name:
FMO 2
Gene namesi
Name:FMO2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000094963.13
HGNCiHGNC:3770 FMO2
MIMi603955 gene
neXtProtiNX_Q99518

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Organism-specific databases

DisGeNETi2327
PharmGKBiPA164741534

Chemistry databases

ChEMBLiCHEMBL3542432

Polymorphism and mutation databases

DMDMi327478599

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001476462 – 471Dimethylaniline monooxygenase [N-oxide-forming] 2Add BLAST470

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1

Post-translational modificationi

The truncated form is probably unable to fold correctly and is rapidly degraded.
FMO2*1 is sumoylated at 'Lys-492'.1 Publication

Keywords - PTMi

Acetylation, Ubl conjugation

Proteomic databases

EPDiQ99518
PaxDbiQ99518
PeptideAtlasiQ99518
PRIDEiQ99518

PTM databases

iPTMnetiQ99518
PhosphoSitePlusiQ99518

Expressioni

Tissue specificityi

Expressed in lung (at protein level). Expressed predominantly in lung, and at a much lesser extent in kidney. Also expressed in fetal lung, but not in liver, kidney and brain.2 Publications

Gene expression databases

BgeeiENSG00000094963
CleanExiHS_FMO2
ExpressionAtlasiQ99518 baseline and differential
GenevisibleiQ99518 HS

Organism-specific databases

HPAiHPA028261

Interactioni

Protein-protein interaction databases

BioGridi108614, 1 interactor
IntActiQ99518, 2 interactors
STRINGi9606.ENSP00000209929

Structurei

3D structure databases

ProteinModelPortaliQ99518
SMRiQ99518
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the FMO family.Curated

Phylogenomic databases

eggNOGiKOG1399 Eukaryota
COG2072 LUCA
HOGENOMiHOG000076537
HOVERGENiHBG002037
InParanoidiQ99518
KOiK00485
OrthoDBiEOG091G0465
PhylomeDBiQ99518
TreeFamiTF105285

Family and domain databases

Gene3Di3.50.50.60, 2 hits
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR000960 Flavin_mOase
IPR020946 Flavin_mOase-like
IPR002254 Flavin_mOase_2
PfamiView protein in Pfam
PF00743 FMO-like, 1 hit
PIRSFiPIRSF000332 FMO, 1 hit
PRINTSiPR00370 FMOXYGENASE
PR01122 FMOXYGENASE2
SUPFAMiSSF51905 SSF51905, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99518-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKKVAVIGA GVSGLISLKC CVDEGLEPTC FERTEDIGGV WRFKENVEDG
60 70 80 90 100
RASIYQSVVT NTSKEMSCFS DFPMPEDFPN FLHNSKLLEY FRIFAKKFDL
110 120 130 140 150
LKYIQFQTTV LSVRKCPDFS SSGQWKVVTQ SNGKEQSAVF DAVMVCSGHH
160 170 180 190 200
ILPHIPLKSF PGMERFKGQY FHSRQYKHPD GFEGKRILVI GMGNSGSDIA
210 220 230 240 250
VELSKNAAQV FISTRHGTWV MSRISEDGYP WDSVFHTRFR SMLRNVLPRT
260 270 280 290 300
AVKWMIEQQM NRWFNHENYG LEPQNKYIMK EPVLNDDVPS RLLCGAIKVK
310 320 330 340 350
STVKELTETS AIFEDGTVEE NIDVIIFATG YSFSFPFLED SLVKVENNMV
360 370 380 390 400
SLYKYIFPAH LDKSTLACIG LIQPLGSIFP TAELQARWVT RVFKGLCSLP
410 420 430 440 450
SERTMMMDII KRNEKRIDLF GESQSQTLQT NYVDYLDELA LEIGAKPDFC
460 470
SLLFKDPKLA VRLYFGPCNS Y
Length:471
Mass (Da):53,644
Last modified:April 5, 2011 - v4
Checksum:iAF87E304131BA703
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti71D → DD in AAW82431 (Ref. 2) Curated1

Polymorphismi

The sequence shown is that of the allele FMO2*2A. There are two alleles; one major, FMO2*2A (truncated form) and one minor, FMO2*1 (full-length form similar to the protein found in other mammals) (PubMed:9804831). A nonsense mutation transforms the Gln-472 of FMO2*1 in a premature stop codon (PubMed:9804831). FMO2*2A occurs in essentially 100% of Caucasians and Asians (PubMed:9804831). FMO2*1 is present at a frequency of approximately 4% to 13% in the sample of population of African descent (PubMed:9804831, PubMed:11042094, PubMed:18794725). FMO2*2A is catalytically inactive (PubMed:9804831).3 Publications

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01484036D → G2 PublicationsCorresponds to variant dbSNP:rs2020870Ensembl.1
Natural variantiVAR_01536159V → I1 PublicationCorresponds to variant dbSNP:rs55708639Ensembl.1
Natural variantiVAR_02218569F → Y1 PublicationCorresponds to variant dbSNP:rs28745274Ensembl.1
Natural variantiVAR_01484181F → S1 PublicationCorresponds to variant dbSNP:rs2020860Ensembl.1
Natural variantiVAR_014842182F → S2 PublicationsCorresponds to variant dbSNP:rs2307492Ensembl.1
Natural variantiVAR_014843195S → L2 PublicationsCorresponds to variant dbSNP:rs2020862Ensembl.1
Natural variantiVAR_015362238R → Q2 PublicationsCorresponds to variant dbSNP:rs28369895Ensembl.1
Natural variantiVAR_022186314E → G1 PublicationCorresponds to variant dbSNP:rs2020863Ensembl.1
Natural variantiVAR_015363391R → T2 PublicationsCorresponds to variant dbSNP:rs28369899Ensembl.1
Natural variantiVAR_014844413N → K2 PublicationsCorresponds to variant dbSNP:rs2020865Ensembl.1
Natural variantiVAR_064887471Y → YQYRLVGPGQWEGARNAIFT QKQRILKPLKTRALKDSSNF SVSFLLKILGLLAVVVAFFC QLQWS in allele FMO2*1. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09267 mRNA Translation: CAA70462.1
AY916056 Genomic DNA Translation: AAW82431.1
BT006979 mRNA Translation: AAP35625.1
AL021026 Genomic DNA No translation available.
CH471067 Genomic DNA Translation: EAW90889.1
BC005894 mRNA Translation: AAH05894.1
RefSeqiNP_001451.2, NM_001460.4
UniGeneiHs.144912

Genome annotation databases

EnsembliENST00000209929; ENSP00000209929; ENSG00000094963
GeneIDi2327
KEGGihsa:2327
UCSCiuc057ngi.1 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiFMO2_HUMAN
AccessioniPrimary (citable) accession number: Q99518
Secondary accession number(s): Q5EBX4, Q86U73, Q9BRX1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: April 5, 2011
Last modified: May 23, 2018
This is version 170 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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