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Protein

D-aspartate oxidase

Gene

DDO

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Selectively catalyzes the oxidative deamination of D-aspartate and its N-methylated derivative, N-methyl D-aspartate.

Catalytic activityi

D-aspartate + H2O + O2 = oxaloacetate + NH3 + H2O2.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei166FAD; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei183FADBy similarity1
Binding sitei223SubstrateBy similarity1
Binding sitei278SubstrateBy similarity1
Binding sitei312FADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi6 – 20FADBy similarityAdd BLAST15
Nucleotide bindingi36 – 37FADBy similarity2
Nucleotide bindingi43 – 44FADBy similarity2
Nucleotide bindingi48 – 50FADBy similarity3
Nucleotide bindingi307 – 311FADBy similarity5

GO - Molecular functioni

  • cofactor binding Source: UniProtKB
  • D-aspartate oxidase activity Source: UniProtKB
  • FAD binding Source: InterPro
  • receptor binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciZFISH:ENSG00000053328-MONOMER.
ReactomeiR-HSA-389661. Glyoxylate metabolism and glycine degradation.
SABIO-RKQ99489.

Names & Taxonomyi

Protein namesi
Recommended name:
D-aspartate oxidase (EC:1.4.3.1)
Short name:
DASOX
Short name:
DDO
Gene namesi
Name:DDO
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:2727. DDO.

Subcellular locationi

GO - Cellular componenti

  • peroxisomal matrix Source: Reactome
  • peroxisome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

Pathology & Biotechi

Organism-specific databases

DisGeNETi8528.
OpenTargetsiENSG00000203797.
PharmGKBiPA27194.

Chemistry databases

ChEMBLiCHEMBL5887.

Polymorphism and mutation databases

BioMutaiDDO.
DMDMi2494037.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001627701 – 341D-aspartate oxidaseAdd BLAST341

Proteomic databases

MaxQBiQ99489.
PaxDbiQ99489.
PeptideAtlasiQ99489.
PRIDEiQ99489.

PTM databases

iPTMnetiQ99489.
PhosphoSitePlusiQ99489.

Expressioni

Gene expression databases

BgeeiENSG00000203797.
CleanExiHS_DDO.
ExpressionAtlasiQ99489. baseline and differential.
GenevisibleiQ99489. HS.

Organism-specific databases

HPAiHPA037525.
HPA037526.

Interactioni

GO - Molecular functioni

  • receptor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi114098. 3 interactors.
IntActiQ99489. 1 interactor.
STRINGi9606.ENSP00000357920.

Chemistry databases

BindingDBiQ99489.

Structurei

3D structure databases

ProteinModelPortaliQ99489.
SMRiQ99489.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi339 – 341Microbody targeting signalSequence analysis3

Sequence similaritiesi

Belongs to the DAMOX/DASOX family.Curated

Phylogenomic databases

eggNOGiKOG3923. Eukaryota.
COG0665. LUCA.
GeneTreeiENSGT00390000018635.
HOGENOMiHOG000046303.
HOVERGENiHBG003493.
InParanoidiQ99489.
KOiK00272.
OMAiWCSSAND.
OrthoDBiEOG091G0G0Y.
PhylomeDBiQ99489.
TreeFamiTF313887.

Family and domain databases

Gene3Di3.50.50.60. 3 hits.
InterProiIPR006181. D-amino_acid_oxidase_CS.
IPR023209. DAO.
IPR006076. FAD-dep_OxRdtase.
IPR023753. FAD/NAD-binding_dom.
[Graphical view]
PANTHERiPTHR11530. PTHR11530. 1 hit.
PfamiPF01266. DAO. 1 hit.
[Graphical view]
PIRSFiPIRSF000189. D-aa_oxidase. 1 hit.
PROSITEiPS00677. DAO. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform DDO-1 (identifier: Q99489-1) [UniParc]FASTAAdd to basket
Also known as: A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDTARIAVVG AGVVGLSTAV CISKLVPRCS VTIISDKFTP DTTSDVAAGM
60 70 80 90 100
LIPHTYPDTP IHTQKQWFRE TFNHLFAIAN SAEAGDAGVH LVSGWQIFQS
110 120 130 140 150
TPTEEVPFWA DVVLGFRKMT EAELKKFPQY VFGQAFTTLK CECPAYLPWL
160 170 180 190 200
EKRIKGSGGW TLTRRIEDLW ELHPSFDIVV NCSGLGSRQL AGDSKIFPVR
210 220 230 240 250
GQVLQVQAPW VEHFIRDGSG LTYIYPGTSH VTLGGTRQKG DWNLSPDAEN
260 270 280 290 300
SREILSRCCA LEPSLHGACN IREKVGLRPY RPGVRLQTEL LARDGQRLPV
310 320 330 340
VHHYGHGSGG ISVHWGTALE AARLVSECVH ALRTPIPKSN L
Length:341
Mass (Da):37,535
Last modified:May 1, 1997 - v1
Checksum:i8CAE7501FB7F215C
GO
Isoform DDO-2 (identifier: Q99489-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     95-153: Missing.

Show »
Length:282
Mass (Da):30,522
Checksum:iDBD01D57E5B72F7A
GO
Isoform 3 (identifier: Q99489-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MRPARHWETRFGARDFGGFQDCFFRDRLM

Show »
Length:369
Mass (Da):40,993
Checksum:i4E4C372EE90DD7C5
GO
Isoform 4 (identifier: Q99489-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MRPARHWETRFGARDFGGFQDCFFRDRLM
     95-153: Missing.

Show »
Length:310
Mass (Da):33,980
Checksum:iE74BBF6D27BE5CB3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti278R → S in BAF85718 (PubMed:14702039).Curated1
Isoform 4 (identifier: Q99489-4)
Sequence conflicti9T → N in DN990727 (Ref. 6) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_036244136F → L in a breast cancer sample; somatic mutation. 1 Publication1
Natural variantiVAR_014939189Q → E.Corresponds to variant rs17622dbSNPEnsembl.1
Natural variantiVAR_014940230H → Y.Corresponds to variant rs17621dbSNPEnsembl.1
Natural variantiVAR_014941255L → R.Corresponds to variant rs17623dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0376641M → MRPARHWETRFGARDFGGFQ DCFFRDRLM in isoform 3 and isoform 4. 3 Publications1
Alternative sequenceiVSP_00126995 – 153Missing in isoform DDO-2 and isoform 4. 2 PublicationsAdd BLAST59

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D89858 mRNA. Translation: BAA14031.1.
AK293029 mRNA. Translation: BAF85718.1.
AL050350 Genomic DNA. Translation: CAI42511.1.
AL050350 Genomic DNA. Translation: CAI42509.1.
AL050350 Genomic DNA. Translation: CAI42510.1.
CH471051 Genomic DNA. Translation: EAW48316.1.
CH471051 Genomic DNA. Translation: EAW48317.1.
BC032786 mRNA. Translation: AAH32786.3.
DN990727 mRNA. No translation available.
CCDSiCCDS5082.1. [Q99489-3]
CCDS5083.1. [Q99489-4]
PIRiJC5438.
JC5439.
RefSeqiNP_003640.2. NM_003649.2. [Q99489-3]
NP_004023.2. NM_004032.2. [Q99489-4]
UniGeneiHs.591348.

Genome annotation databases

EnsembliENST00000368923; ENSP00000357919; ENSG00000203797. [Q99489-4]
ENST00000368924; ENSP00000357920; ENSG00000203797. [Q99489-3]
GeneIDi8528.
KEGGihsa:8528.
UCSCiuc003puc.4. human. [Q99489-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D89858 mRNA. Translation: BAA14031.1.
AK293029 mRNA. Translation: BAF85718.1.
AL050350 Genomic DNA. Translation: CAI42511.1.
AL050350 Genomic DNA. Translation: CAI42509.1.
AL050350 Genomic DNA. Translation: CAI42510.1.
CH471051 Genomic DNA. Translation: EAW48316.1.
CH471051 Genomic DNA. Translation: EAW48317.1.
BC032786 mRNA. Translation: AAH32786.3.
DN990727 mRNA. No translation available.
CCDSiCCDS5082.1. [Q99489-3]
CCDS5083.1. [Q99489-4]
PIRiJC5438.
JC5439.
RefSeqiNP_003640.2. NM_003649.2. [Q99489-3]
NP_004023.2. NM_004032.2. [Q99489-4]
UniGeneiHs.591348.

3D structure databases

ProteinModelPortaliQ99489.
SMRiQ99489.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114098. 3 interactors.
IntActiQ99489. 1 interactor.
STRINGi9606.ENSP00000357920.

Chemistry databases

BindingDBiQ99489.
ChEMBLiCHEMBL5887.

PTM databases

iPTMnetiQ99489.
PhosphoSitePlusiQ99489.

Polymorphism and mutation databases

BioMutaiDDO.
DMDMi2494037.

Proteomic databases

MaxQBiQ99489.
PaxDbiQ99489.
PeptideAtlasiQ99489.
PRIDEiQ99489.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000368923; ENSP00000357919; ENSG00000203797. [Q99489-4]
ENST00000368924; ENSP00000357920; ENSG00000203797. [Q99489-3]
GeneIDi8528.
KEGGihsa:8528.
UCSCiuc003puc.4. human. [Q99489-1]

Organism-specific databases

CTDi8528.
DisGeNETi8528.
GeneCardsiDDO.
HGNCiHGNC:2727. DDO.
HPAiHPA037525.
HPA037526.
MIMi124450. gene.
neXtProtiNX_Q99489.
OpenTargetsiENSG00000203797.
PharmGKBiPA27194.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3923. Eukaryota.
COG0665. LUCA.
GeneTreeiENSGT00390000018635.
HOGENOMiHOG000046303.
HOVERGENiHBG003493.
InParanoidiQ99489.
KOiK00272.
OMAiWCSSAND.
OrthoDBiEOG091G0G0Y.
PhylomeDBiQ99489.
TreeFamiTF313887.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000053328-MONOMER.
ReactomeiR-HSA-389661. Glyoxylate metabolism and glycine degradation.
SABIO-RKQ99489.

Miscellaneous databases

GeneWikiiDDO_(gene).
GenomeRNAii8528.
PROiQ99489.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000203797.
CleanExiHS_DDO.
ExpressionAtlasiQ99489. baseline and differential.
GenevisibleiQ99489. HS.

Family and domain databases

Gene3Di3.50.50.60. 3 hits.
InterProiIPR006181. D-amino_acid_oxidase_CS.
IPR023209. DAO.
IPR006076. FAD-dep_OxRdtase.
IPR023753. FAD/NAD-binding_dom.
[Graphical view]
PANTHERiPTHR11530. PTHR11530. 1 hit.
PfamiPF01266. DAO. 1 hit.
[Graphical view]
PIRSFiPIRSF000189. D-aa_oxidase. 1 hit.
PROSITEiPS00677. DAO. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOXDD_HUMAN
AccessioniPrimary (citable) accession number: Q99489
Secondary accession number(s): A8KAG4
, Q5JXM4, Q5JXM5, Q5JXM6, Q8N552
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 1997
Last modified: November 2, 2016
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.