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Q99489 (OXDD_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-aspartate oxidase

Short name=DASOX
Short name=DDO
EC=1.4.3.1
Gene names
Name:DDO
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length341 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Selectively catalyzes the oxidative deamination of D-aspartate and its N-methylated derivative, N-methyl D-aspartate.

Catalytic activity

D-aspartate + H2O + O2 = oxaloacetate + NH3 + H2O2.

Cofactor

FAD or 6-hydroxyflavin adenine dinucleotide.

Subcellular location

Peroxisome.

Sequence similarities

Belongs to the DAMOX/DASOX family.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform DDO-1 (identifier: Q99489-1)

Also known as: A;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform DDO-2 (identifier: Q99489-2)

The sequence of this isoform differs from the canonical sequence as follows:
     95-153: Missing.
Isoform 3 (identifier: Q99489-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MRPARHWETRFGARDFGGFQDCFFRDRLM
Isoform 4 (identifier: Q99489-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MRPARHWETRFGARDFGGFQDCFFRDRLM
     95-153: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 341341D-aspartate oxidase
PRO_0000162770

Regions

Nucleotide binding6 – 2015FAD By similarity
Nucleotide binding36 – 372FAD By similarity
Nucleotide binding43 – 442FAD By similarity
Nucleotide binding48 – 503FAD By similarity
Nucleotide binding307 – 3115FAD By similarity
Motif339 – 3413Microbody targeting signal Potential

Sites

Binding site1661FAD; via amide nitrogen and carbonyl oxygen By similarity
Binding site1831FAD By similarity
Binding site2231Substrate By similarity
Binding site2781Substrate By similarity
Binding site3121FAD By similarity

Natural variations

Alternative sequence11M → MRPARHWETRFGARDFGGFQ DCFFRDRLM in isoform 3 and isoform 4.
VSP_037664
Alternative sequence95 – 15359Missing in isoform DDO-2 and isoform 4.
VSP_001269
Natural variant1361F → L in a breast cancer sample; somatic mutation. Ref.7
VAR_036244
Natural variant1891Q → E.
Corresponds to variant rs17622 [ dbSNP | Ensembl ].
VAR_014939
Natural variant2301H → Y.
Corresponds to variant rs17621 [ dbSNP | Ensembl ].
VAR_014940
Natural variant2551L → R.
Corresponds to variant rs17623 [ dbSNP | Ensembl ].
VAR_014941

Experimental info

Sequence conflict2781R → S in BAF85718. Ref.2
Isoform 4:
Sequence conflict91T → N in DN990727. Ref.6

Sequences

Sequence LengthMass (Da)Tools
Isoform DDO-1 (A) [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 8CAE7501FB7F215C

FASTA34137,535
        10         20         30         40         50         60 
MDTARIAVVG AGVVGLSTAV CISKLVPRCS VTIISDKFTP DTTSDVAAGM LIPHTYPDTP 

        70         80         90        100        110        120 
IHTQKQWFRE TFNHLFAIAN SAEAGDAGVH LVSGWQIFQS TPTEEVPFWA DVVLGFRKMT 

       130        140        150        160        170        180 
EAELKKFPQY VFGQAFTTLK CECPAYLPWL EKRIKGSGGW TLTRRIEDLW ELHPSFDIVV 

       190        200        210        220        230        240 
NCSGLGSRQL AGDSKIFPVR GQVLQVQAPW VEHFIRDGSG LTYIYPGTSH VTLGGTRQKG 

       250        260        270        280        290        300 
DWNLSPDAEN SREILSRCCA LEPSLHGACN IREKVGLRPY RPGVRLQTEL LARDGQRLPV 

       310        320        330        340 
VHHYGHGSGG ISVHWGTALE AARLVSECVH ALRTPIPKSN L 

« Hide

Isoform DDO-2 [UniParc].

Checksum: DBD01D57E5B72F7A
Show »

FASTA28230,522
Isoform 3 [UniParc].

Checksum: 4E4C372EE90DD7C5
Show »

FASTA36940,993
Isoform 4 [UniParc].

Checksum: E74BBF6D27BE5CB3
Show »

FASTA31033,980

References

« Hide 'large scale' references
[1]"Structural and functional characterization of the human brain D-aspartate oxidase."
Setoyama C., Miura R.
J. Biochem. 121:798-803(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS DDO-1 AND DDO-2).
Tissue: Brain.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Uterus.
[3]"The DNA sequence and analysis of human chromosome 6."
Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Brain.
[6]"High-throughput cloning of full-length human cDNAs directly from cDNA libraries optimized for large and rare transcripts."
Birkett C., Cho J., Gau Y., Hamer R., Kelly S., Kovacs K., Liu L., Liu X., Porter J., Sachs A., Shu Y., Sun Z., Wong J., Wu M., Zhang X., Jay G., He W.
Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-206 (ISOFORM 4).
[7]"The consensus coding sequences of human breast and colorectal cancers."
Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. expand/collapse author list , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.
Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT [LARGE SCALE ANALYSIS] LEU-136.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D89858 mRNA. Translation: BAA14031.1.
AK293029 mRNA. Translation: BAF85718.1.
AL050350 Genomic DNA. Translation: CAI42511.1.
AL050350 Genomic DNA. Translation: CAI42509.1.
AL050350 Genomic DNA. Translation: CAI42510.1.
CH471051 Genomic DNA. Translation: EAW48316.1.
CH471051 Genomic DNA. Translation: EAW48317.1.
BC032786 mRNA. Translation: AAH32786.3.
DN990727 mRNA. No translation available.
PIRJC5438.
JC5439.
RefSeqNP_003640.2. NM_003649.2.
NP_004023.2. NM_004032.2.
UniGeneHs.591348.

3D structure databases

ProteinModelPortalQ99489.
SMRQ99489. Positions 3-324.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid114098. 3 interactions.
IntActQ99489. 1 interaction.
STRING9606.ENSP00000357920.

Chemistry

BindingDBQ99489.
ChEMBLCHEMBL5887.

PTM databases

PhosphoSiteQ99489.

Polymorphism databases

DMDM2494037.

Proteomic databases

PaxDbQ99489.
PRIDEQ99489.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000368923; ENSP00000357919; ENSG00000203797. [Q99489-4]
ENST00000368924; ENSP00000357920; ENSG00000203797. [Q99489-3]
GeneID8528.
KEGGhsa:8528.
UCSCuc003puc.3. human. [Q99489-3]
uc003pud.3. human. [Q99489-4]

Organism-specific databases

CTD8528.
GeneCardsGC06M110672.
HGNCHGNC:2727. DDO.
HPAHPA037525.
HPA037526.
MIM124450. gene.
neXtProtNX_Q99489.
PharmGKBPA27194.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0665.
HOGENOMHOG000046303.
HOVERGENHBG003493.
InParanoidQ99489.
KOK00272.
OMAYANDESG.
OrthoDBEOG7B31NB.
PhylomeDBQ99489.
TreeFamTF313887.

Enzyme and pathway databases

SABIO-RKQ99489.

Gene expression databases

ArrayExpressQ99489.
BgeeQ99489.
CleanExHS_DDO.
GenevestigatorQ99489.

Family and domain databases

InterProIPR023209. D-aa_oxidase.
IPR006181. D-amino_acid_oxidase_CS.
IPR006076. FAD-dep_OxRdtase.
[Graphical view]
PANTHERPTHR11530. PTHR11530. 1 hit.
PfamPF01266. DAO. 1 hit.
[Graphical view]
PIRSFPIRSF000189. D-aa_oxidase. 1 hit.
PROSITEPS00677. DAO. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiDDO_(gene).
GenomeRNAi8528.
NextBio31934.
PROQ99489.
SOURCESearch...

Entry information

Entry nameOXDD_HUMAN
AccessionPrimary (citable) accession number: Q99489
Secondary accession number(s): A8KAG4 expand/collapse secondary AC list , Q5JXM4, Q5JXM5, Q5JXM6, Q8N552
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 1997
Last modified: April 16, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 6

Human chromosome 6: entries, gene names and cross-references to MIM