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Protein

D-aspartate oxidase

Gene

DDO

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Selectively catalyzes the oxidative deamination of D-aspartate and its N-methylated derivative, N-methyl D-aspartate.

Catalytic activityi

D-aspartate + H2O + O2 = oxaloacetate + NH3 + H2O2.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei166FAD; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei183FADBy similarity1
Binding sitei223SubstrateBy similarity1
Binding sitei278SubstrateBy similarity1
Binding sitei312FADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi6 – 20FADBy similarityAdd BLAST15
Nucleotide bindingi36 – 37FADBy similarity2
Nucleotide bindingi43 – 44FADBy similarity2
Nucleotide bindingi48 – 50FADBy similarity3
Nucleotide bindingi307 – 311FADBy similarity5

GO - Molecular functioni

  • cofactor binding Source: UniProtKB
  • D-aspartate oxidase activity Source: UniProtKB
  • FAD binding Source: InterPro
  • receptor binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein

Enzyme and pathway databases

ReactomeiR-HSA-389661 Glyoxylate metabolism and glycine degradation
SABIO-RKiQ99489

Names & Taxonomyi

Protein namesi
Recommended name:
D-aspartate oxidase (EC:1.4.3.1)
Short name:
DASOX
Short name:
DDO
Gene namesi
Name:DDO
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000203797.9
HGNCiHGNC:2727 DDO
MIMi124450 gene
neXtProtiNX_Q99489

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Peroxisome

Pathology & Biotechi

Organism-specific databases

DisGeNETi8528
OpenTargetsiENSG00000203797
PharmGKBiPA27194

Chemistry databases

ChEMBLiCHEMBL5887

Polymorphism and mutation databases

BioMutaiDDO
DMDMi2494037

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001627701 – 341D-aspartate oxidaseAdd BLAST341

Proteomic databases

MaxQBiQ99489
PaxDbiQ99489
PeptideAtlasiQ99489
PRIDEiQ99489

PTM databases

iPTMnetiQ99489
PhosphoSitePlusiQ99489

Expressioni

Gene expression databases

BgeeiENSG00000203797
CleanExiHS_DDO
ExpressionAtlasiQ99489 baseline and differential
GenevisibleiQ99489 HS

Organism-specific databases

HPAiHPA037525
HPA037526

Interactioni

GO - Molecular functioni

  • receptor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi1140983 interactors.
IntActiQ99489 1 interactor.
STRINGi9606.ENSP00000357920

Chemistry databases

BindingDBiQ99489

Structurei

3D structure databases

ProteinModelPortaliQ99489
SMRiQ99489
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi339 – 341Microbody targeting signalSequence analysis3

Sequence similaritiesi

Belongs to the DAMOX/DASOX family.Curated

Phylogenomic databases

eggNOGiKOG3923 Eukaryota
COG0665 LUCA
GeneTreeiENSGT00390000018635
HOGENOMiHOG000046303
HOVERGENiHBG003493
InParanoidiQ99489
KOiK00272
OMAiHLVSGWQ
OrthoDBiEOG091G0G0Y
PhylomeDBiQ99489
TreeFamiTF313887

Family and domain databases

InterProiView protein in InterPro
IPR006181 D-amino_acid_oxidase_CS
IPR023209 DAO
IPR006076 FAD-dep_OxRdtase
PANTHERiPTHR11530 PTHR11530, 1 hit
PfamiView protein in Pfam
PF01266 DAO, 1 hit
PIRSFiPIRSF000189 D-aa_oxidase, 1 hit
PROSITEiView protein in PROSITE
PS00677 DAO, 1 hit

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform DDO-1 (identifier: Q99489-1) [UniParc]FASTAAdd to basket
Also known as: A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDTARIAVVG AGVVGLSTAV CISKLVPRCS VTIISDKFTP DTTSDVAAGM
60 70 80 90 100
LIPHTYPDTP IHTQKQWFRE TFNHLFAIAN SAEAGDAGVH LVSGWQIFQS
110 120 130 140 150
TPTEEVPFWA DVVLGFRKMT EAELKKFPQY VFGQAFTTLK CECPAYLPWL
160 170 180 190 200
EKRIKGSGGW TLTRRIEDLW ELHPSFDIVV NCSGLGSRQL AGDSKIFPVR
210 220 230 240 250
GQVLQVQAPW VEHFIRDGSG LTYIYPGTSH VTLGGTRQKG DWNLSPDAEN
260 270 280 290 300
SREILSRCCA LEPSLHGACN IREKVGLRPY RPGVRLQTEL LARDGQRLPV
310 320 330 340
VHHYGHGSGG ISVHWGTALE AARLVSECVH ALRTPIPKSN L
Length:341
Mass (Da):37,535
Last modified:May 1, 1997 - v1
Checksum:i8CAE7501FB7F215C
GO
Isoform DDO-2 (identifier: Q99489-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     95-153: Missing.

Show »
Length:282
Mass (Da):30,522
Checksum:iDBD01D57E5B72F7A
GO
Isoform 3 (identifier: Q99489-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MRPARHWETRFGARDFGGFQDCFFRDRLM

Show »
Length:369
Mass (Da):40,993
Checksum:i4E4C372EE90DD7C5
GO
Isoform 4 (identifier: Q99489-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MRPARHWETRFGARDFGGFQDCFFRDRLM
     95-153: Missing.

Show »
Length:310
Mass (Da):33,980
Checksum:iE74BBF6D27BE5CB3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti278R → S in BAF85718 (PubMed:14702039).Curated1
Isoform 4 (identifier: Q99489-4)
Sequence conflicti9T → N in DN990727 (Ref. 6) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_036244136F → L in a breast cancer sample; somatic mutation. 1 Publication1
Natural variantiVAR_014939189Q → E. Corresponds to variant dbSNP:rs17622Ensembl.1
Natural variantiVAR_014940230H → Y. Corresponds to variant dbSNP:rs17621Ensembl.1
Natural variantiVAR_014941255L → R. Corresponds to variant dbSNP:rs17623Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0376641M → MRPARHWETRFGARDFGGFQ DCFFRDRLM in isoform 3 and isoform 4. 3 Publications1
Alternative sequenceiVSP_00126995 – 153Missing in isoform DDO-2 and isoform 4. 2 PublicationsAdd BLAST59

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D89858 mRNA Translation: BAA14031.1
AK293029 mRNA Translation: BAF85718.1
AL050350 Genomic DNA No translation available.
CH471051 Genomic DNA Translation: EAW48316.1
CH471051 Genomic DNA Translation: EAW48317.1
BC032786 mRNA Translation: AAH32786.3
DN990727 mRNA No translation available.
CCDSiCCDS5082.1 [Q99489-3]
CCDS5083.1 [Q99489-4]
PIRiJC5438
JC5439
RefSeqiNP_003640.2, NM_003649.2 [Q99489-3]
NP_004023.2, NM_004032.2 [Q99489-4]
UniGeneiHs.591348

Genome annotation databases

EnsembliENST00000368923; ENSP00000357919; ENSG00000203797 [Q99489-4]
ENST00000368924; ENSP00000357920; ENSG00000203797 [Q99489-3]
GeneIDi8528
KEGGihsa:8528
UCSCiuc003puc.4 human [Q99489-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiOXDD_HUMAN
AccessioniPrimary (citable) accession number: Q99489
Secondary accession number(s): A8KAG4
, Q5JXM4, Q5JXM5, Q5JXM6, Q8N552
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 1997
Last modified: February 28, 2018
This is version 156 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome