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Q99487

- PAFA2_HUMAN

UniProt

Q99487 - PAFA2_HUMAN

Protein

Platelet-activating factor acetylhydrolase 2, cytoplasmic

Gene

PAFAH2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
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    Functioni

    Has a marked selectivity for phospholipids with short acyl chains at the sn-2 position. May share a common physiologic function with the plasma-type enzyme.

    Catalytic activityi

    1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate.

    Enzyme regulationi

    Inhibited by phenylmethanesulfonyl fluoride, 3,4,dichloroisocoumarin, diisopropyl fluorophosphate (DFP) and diethyl p-nitrophenyl phosphate (DENP).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei236 – 2361NucleophileBy similarity
    Active sitei259 – 2591Charge relay systemPROSITE-ProRule annotation
    Active sitei314 – 3141Charge relay systemPROSITE-ProRule annotation

    GO - Molecular functioni

    1. 1-alkyl-2-acetylglycerophosphocholine esterase activity Source: UniProtKB-EC
    2. phospholipid binding Source: ProtInc

    GO - Biological processi

    1. lipid catabolic process Source: UniProtKB-KW
    2. lipid metabolic process Source: ProtInc
    3. negative regulation of apoptotic process Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Enzyme and pathway databases

    BRENDAi3.1.1.47. 2681.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Platelet-activating factor acetylhydrolase 2, cytoplasmic (EC:3.1.1.47)
    Alternative name(s):
    Serine-dependent phospholipase A2
    Short name:
    SD-PLA2
    Short name:
    hSD-PLA2
    Gene namesi
    Name:PAFAH2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 1

    Organism-specific databases

    HGNCiHGNC:8579. PAFAH2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA32910.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 392392Platelet-activating factor acetylhydrolase 2, cytoplasmicPRO_0000090383Add
    BLAST

    Proteomic databases

    MaxQBiQ99487.
    PaxDbiQ99487.
    PRIDEiQ99487.

    PTM databases

    PhosphoSiteiQ99487.

    Expressioni

    Tissue specificityi

    Broadly expressed in different tissues, but high in B- and T-lymphocytes. In brain, expression is restricted to amygdala and frontal cortex.

    Gene expression databases

    ArrayExpressiQ99487.
    BgeeiQ99487.
    CleanExiHS_PAFAH2.
    GenevestigatoriQ99487.

    Organism-specific databases

    HPAiHPA018157.

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    STRINGi9606.ENSP00000363400.

    Structurei

    3D structure databases

    ProteinModelPortaliQ99487.
    SMRiQ99487. Positions 10-384.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the serine esterase family.Curated

    Phylogenomic databases

    eggNOGiCOG4188.
    HOGENOMiHOG000008053.
    HOVERGENiHBG001322.
    InParanoidiQ99487.
    KOiK01062.
    OMAiQFRCAVA.
    OrthoDBiEOG7MSMQ5.
    PhylomeDBiQ99487.
    TreeFamiTF313831.

    Family and domain databases

    Gene3Di3.40.50.1820. 2 hits.
    InterProiIPR029058. AB_hydrolase.
    IPR005065. PAF_acetylhydro.
    IPR016715. PAF_acetylhydro_eukaryote.
    [Graphical view]
    PANTHERiPTHR10272. PTHR10272. 1 hit.
    PfamiPF03403. PAF-AH_p_II. 1 hit.
    [Graphical view]
    PIRSFiPIRSF018169. PAF_acetylhydrolase. 1 hit.
    SUPFAMiSSF53474. SSF53474. 2 hits.
    PROSITEiPS00120. LIPASE_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q99487-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGVNQSVGFP PVTGPHLVGC GDVMEGQNLQ GSFFRLFYPC QKAEETMEQP    50
    LWIPRYEYCT GLAEYLQFNK RCGGLLFNLA VGSCRLPVSW NGPFKTKDSG 100
    YPLIIFSHGL GAFRTLYSAF CMELASRGFV VAVPEHRDRS AATTYFCKQA 150
    PEENQPTNES LQEEWIPFRR VEEGEKEFHV RNPQVHQRVS ECLRVLKILQ 200
    EVTAGQTVFN ILPGGLDLMT LKGNIDMSRV AVMGHSFGGA TAILALAKET 250
    QFRCAVALDA WMFPLERDFY PKARGPVFFI NTEKFQTMES VNLMKKICAQ 300
    HEQSRIITVL GSVHRSQTDF AFVTGNLIGK FFSTETRGSL DPYEGQEVMV 350
    RAMLAFLQKH LDLKEDYNQW NNLIEGIGPS LTPGAPHHLS SL 392
    Length:392
    Mass (Da):44,036
    Last modified:May 1, 1997 - v1
    Checksum:i690FB7E6F5B68317
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti212 – 2121L → F in AAC39707. (PubMed:9494101)Curated

    Mass spectrometryi

    Molecular mass is 44162 Da from positions 1 - 392. Determined by ESI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D87845 mRNA. Translation: BAA13468.1.
    U89386 mRNA. Translation: AAC39707.1.
    AL592064 Genomic DNA. Translation: CAI10970.1.
    CH471059 Genomic DNA. Translation: EAX07852.1.
    CH471059 Genomic DNA. Translation: EAX07853.1.
    BC001158 mRNA. Translation: AAH01158.1.
    CCDSiCCDS270.1.
    RefSeqiNP_000428.2. NM_000437.3.
    XP_006710733.1. XM_006710670.1.
    UniGeneiHs.477083.

    Genome annotation databases

    EnsembliENST00000374282; ENSP00000363400; ENSG00000158006.
    ENST00000374284; ENSP00000363402; ENSG00000158006.
    GeneIDi5051.
    KEGGihsa:5051.
    UCSCiuc001bld.4. human.

    Polymorphism databases

    DMDMi6647691.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D87845 mRNA. Translation: BAA13468.1 .
    U89386 mRNA. Translation: AAC39707.1 .
    AL592064 Genomic DNA. Translation: CAI10970.1 .
    CH471059 Genomic DNA. Translation: EAX07852.1 .
    CH471059 Genomic DNA. Translation: EAX07853.1 .
    BC001158 mRNA. Translation: AAH01158.1 .
    CCDSi CCDS270.1.
    RefSeqi NP_000428.2. NM_000437.3.
    XP_006710733.1. XM_006710670.1.
    UniGenei Hs.477083.

    3D structure databases

    ProteinModelPortali Q99487.
    SMRi Q99487. Positions 10-384.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9606.ENSP00000363400.

    PTM databases

    PhosphoSitei Q99487.

    Polymorphism databases

    DMDMi 6647691.

    Proteomic databases

    MaxQBi Q99487.
    PaxDbi Q99487.
    PRIDEi Q99487.

    Protocols and materials databases

    DNASUi 5051.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000374282 ; ENSP00000363400 ; ENSG00000158006 .
    ENST00000374284 ; ENSP00000363402 ; ENSG00000158006 .
    GeneIDi 5051.
    KEGGi hsa:5051.
    UCSCi uc001bld.4. human.

    Organism-specific databases

    CTDi 5051.
    GeneCardsi GC01M026286.
    HGNCi HGNC:8579. PAFAH2.
    HPAi HPA018157.
    MIMi 602344. gene.
    neXtProti NX_Q99487.
    PharmGKBi PA32910.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG4188.
    HOGENOMi HOG000008053.
    HOVERGENi HBG001322.
    InParanoidi Q99487.
    KOi K01062.
    OMAi QFRCAVA.
    OrthoDBi EOG7MSMQ5.
    PhylomeDBi Q99487.
    TreeFami TF313831.

    Enzyme and pathway databases

    BRENDAi 3.1.1.47. 2681.

    Miscellaneous databases

    ChiTaRSi PAFAH2. human.
    GeneWikii PAFAH2.
    GenomeRNAii 5051.
    NextBioi 19464.
    PROi Q99487.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q99487.
    Bgeei Q99487.
    CleanExi HS_PAFAH2.
    Genevestigatori Q99487.

    Family and domain databases

    Gene3Di 3.40.50.1820. 2 hits.
    InterProi IPR029058. AB_hydrolase.
    IPR005065. PAF_acetylhydro.
    IPR016715. PAF_acetylhydro_eukaryote.
    [Graphical view ]
    PANTHERi PTHR10272. PTHR10272. 1 hit.
    Pfami PF03403. PAF-AH_p_II. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF018169. PAF_acetylhydrolase. 1 hit.
    SUPFAMi SSF53474. SSF53474. 2 hits.
    PROSITEi PS00120. LIPASE_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA cloning and expression of intracellular platelet-activating factor (PAF) acetylhydrolase II. Its homology with plasma PAF acetylhydrolase."
      Hattori K., Adachi H., Matsuzawa A., Yamamoto K., Tsujimoto M., Aoki J., Hattori M., Arai H., Inoue K.
      J. Biol. Chem. 271:33032-33038(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "Expression, purification and characterization of a human serine-dependent phospholipase A2 with high specificity for oxidized phospholipids and platelet activating factor."
      Rice S.Q.J., Southan C., Boyd H.F., Terrett J.A., Macphee C.H., Moores K., Gloger I.S., Tew D.G.
      Biochem. J. 330:1309-1315(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-5, MASS SPECTROMETRY.
      Tissue: Prostate.
    3. "The DNA sequence and biological annotation of human chromosome 1."
      Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
      , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
      Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Eye.
    6. Cited for: REVIEW.

    Entry informationi

    Entry nameiPAFA2_HUMAN
    AccessioniPrimary (citable) accession number: Q99487
    Secondary accession number(s): D3DPK1, O15458, Q5SY02
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 126 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3