Q99471 (PFD5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Prefoldin subunit 5

Alternative name(s):
C-Myc-binding protein Mm-1
Myc modulator 1

Gene names

Name:	PFDN5
Synonyms:	MM1, PFD5

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	154 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins. Represses the transcriptional activity of MYC. Ref.9
Subunit structure	Heterohexamer of two PFD-alpha type and four PFD-beta type subunits. Binds to MYC; interacts with its N-terminal domain.
Subcellular location	Isoform 1: Nucleus Ref.3. Isoform 2: Cytoplasm Ref.3. Isoform 3: Nucleus Ref.3.
Tissue specificity	Highly expressed in pancreas and skeletal muscle and moderately in other tissues.
Sequence similarities	Belongs to the prefoldin subunit alpha family.
Sequence caution	The sequence BAA14006.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Molecular function	Chaperone
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	'de novo' posttranslational protein folding Traceable author statement. Source: Reactome negative regulation of canonical Wnt receptor signaling pathway Inferred from mutant phenotype PubMed 18281035. Source: BHF-UCL negative regulation of transcription, DNA-dependent Inferred from mutant phenotype PubMed 18281035. Source: BHF-UCL retina development in camera-type eye Inferred from electronic annotation. Source: Compara
Cellular_component	cytoplasm Traceable author statement PubMed 16130169. Source: UniProtKB nucleus Traceable author statement Ref.1. Source: ProtInc prefoldin complex Inferred from electronic annotation. Source: InterPro
Molecular_function	transcription corepressor activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
vIRF-3	Q9DUN1	8	EBI-357275,EBI-1647907	From a different organism.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: Q99471-1)

Also known as: MM1-alpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q99471-2)

Also known as: MM1-beta;

The sequence of this isoform differs from the canonical sequence as follows:
     59-154: GKELLVPLTS...LGAAQATAKA → DVCPWEAA

Note: Does not repress transcription activity of MYC.

Isoform 3 (identifier: Q99471-3)

Also known as: MM1-gamma;

The sequence of this isoform differs from the canonical sequence as follows:
     25-69: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 154	154	Prefoldin subunit 5		PRO_0000153661
Amino acid modifications
	Modified residue	42	1	N6-acetyllysine Ref.10
Natural variations
	Alternative sequence	25 – 69	45	Missing in isoform 3.		VSP_043103
	Alternative sequence	59 – 154	96	GKELL…ATAKA → DVCPWEAA in isoform 2.		VSP_043104

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (MM1-alpha) [UniParc]. Last modified January 11, 2001. Version 2. Checksum: 0DA1F3644548CB14	FASTA	154	17,328
	10 20 30 40 50 60 MAQSINITEL NLPQLEMLKN QLDQEVEFLS TSIAQLKVVQ TKYVEAKDCL NVLNKSNEGK 70 80 90 100 110 120 ELLVPLTSSM YVPGKLHDVE HVLIDVGTGY YVEKTAEDAK DFFKRKIDFL TKQMEKIQPA 130 140 150 LQEKHAMKQA VMEMMSQKIQ QLTALGAAQA TAKA
	Isoform 2 (MM1-beta) [UniParc]. Checksum: C07E3472E5BD2A76	FASTA	66	7,481
	10 20 30 40 50 60 MAQSINITEL NLPQLEMLKN QLDQEVEFLS TSIAQLKVVQ TKYVEAKDCL NVLNKSNEDV CPWEAA
	Isoform 3 (MM1-gamma) [UniParc]. Checksum: 899AFF472DCC00D7	FASTA	109	12,379
	10 20 30 40 50 60 MAQSINITEL NLPQLEMLKN QLDQMYVPGK LHDVEHVLID VGTGYYVEKT AEDAKDFFKR 70 80 90 100 KIDFLTKQME KIQPALQEKH AMKQAVMEMM SQKIQQLTAL GAAQATAKA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"MM-1, a novel c-Myc-associating protein that represses transcriptional activity of c-Myc." Mori K., Maeda Y., Kitaura H., Taira T., Iguchi-Ariga S.M., Ariga H. J. Biol. Chem. 273:29794-29800(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Brain.
[2]	"MM-1, a c-Myc-binding protein, is a candidate for a tumor suppressor in leukemia/lymphoma and tongue cancer." Fujioka Y., Taira T., Maeda Y., Tanaka S., Nishihara H., Iguchi-Ariga S.M.M., Nagashima K., Ariga H. J. Biol. Chem. 276:45137-45144(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1). Tissue: Blood and Fibroblast.
[3]	"Distinct localizations and repression activities of MM-1 isoforms toward c-Myc." Hagio Y., Kimura Y., Taira T., Fujioka Y., Iguchi-Ariga S.M.M., Ariga H. J. Cell. Biochem. 97:145-155(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3), SUBCELLULAR LOCATION, ALTERNATIVE SPLICING. Tissue: B-cell and Placenta.
[4]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Thymus.
[6]	"The finished DNA sequence of human chromosome 12." Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R. , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A. Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Placenta.
[9]	"Prefoldin, a chaperone that delivers unfolded proteins to cytosolic chaperonin." Vainberg I.E., Lewis S.A., Rommelaere H., Ampe C., Vandekerckhove J., Klein H.L., Cowan N.J. Cell 93:863-873(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[10]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-42, MASS SPECTROMETRY.
[11]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	D89667 mRNA. Translation: BAA14006.1. Different initiation. AB055802 Genomic DNA. Translation: BAB32643.1. AB055803 mRNA. Translation: BAB32644.1. AB055804 mRNA. Translation: BAB32645.1. AB055805 mRNA. Translation: BAB32646.1. BT007195 mRNA. Translation: AAP35859.1. AK292623 mRNA. Translation: BAF85312.1. AC073611 Genomic DNA. No translation available. CH471054 Genomic DNA. Translation: EAW96683.1. CH471054 Genomic DNA. Translation: EAW96685.1. BC003373 mRNA. No translation available. BC062671 mRNA. Translation: AAH62671.1.
IPI	IPI00015361. IPI00028183. IPI00030303.
RefSeq	NP_002615.2. NM_002624.3. NP_665904.1. NM_145897.2.
UniGene	Hs.655327.
3D structure databases
ProteinModelPortal	Q99471.
SMR	Q99471. Positions 11-144.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-28155N.
IntAct	Q99471. 9 interactions.
MINT	MINT-5003456.
STRING	9606.ENSP00000334188.
PTM databases
PhosphoSite	Q99471.
Polymorphism databases
DMDM	12231038.
2D gel databases
OGP	Q99471.
Proteomic databases
PaxDb	Q99471.
PeptideAtlas	Q99471.
PRIDE	Q99471.
Protocols and materials databases
DNASU	5204.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000243040; ENSP00000243040; ENSG00000123349. ENST00000334478; ENSP00000334188; ENSG00000123349. ENST00000351500; ENSP00000266964; ENSG00000123349. ENST00000551018; ENSP00000447942; ENSG00000123349.
GeneID	5204.
KEGG	hsa:5204.
UCSC	uc001scl.3. human. uc001scm.3. human.
Organism-specific databases
CTD	5204.
GeneCards	GC12P053689.
HGNC	HGNC:8869. PFDN5.
HPA	HPA008587.
MIM	604899. gene.
neXtProt	NX_Q99471.
PharmGKB	PA33210.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG1730.
HOGENOM	HOG000207562.
HOVERGEN	HBG004539.
InParanoid	Q99471.
KO	K04797.
OMA	KDYFKRR.
OrthoDB	EOG415GFQ.
PhylomeDB	Q99471.
Enzyme and pathway databases
Reactome	REACT_17015. Metabolism of proteins.
Gene expression databases
ArrayExpress	Q99471.
Bgee	Q99471.
CleanEx	HS_PFDN5.
Genevestigator	Q99471.
GermOnline	ENSG00000123349. Homo sapiens.
Family and domain databases
InterPro	IPR011599. PFD_alpha. IPR027236. PFDN5. IPR009053. Prefoldin. IPR004127. Prefoldin_subunit. [Graphical view]
PANTHER	PTHR12674:SF0. PTHR12674:SF0. 1 hit.
Pfam	PF02996. Prefoldin. 1 hit. [Graphical view]
SUPFAM	SSF46579. Prefoldin. 1 hit.
TIGRFAMs	TIGR00293. TIGR00293. 1 hit.
ProtoNet	Search...
Other
ChiTaRS	PFDN5. human.
GenomeRNAi	5204.
NextBio	20126.
SOURCE	Search...

Entry information

Entry name

PFD5_HUMAN

Accession

Primary (citable) accession number: Q99471
Secondary accession number(s): A8K9A8 , Q54AA8, Q9C083, Q9C084

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 11, 2001
Last modified:	May 29, 2013
This is version 116 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.