Q99447 (PCY2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ethanolamine-phosphate cytidylyltransferase
EC=2.7.7.14

Alternative name(s):
CTP:phosphoethanolamine cytidylyltransferase
Phosphorylethanolamine transferase

Gene names

Name:

PCYT2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	389 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays an important role in the biosynthesis of the phospholipid phosphatidylethanolamine. Catalyzes the formation of CDP-ethanolamine.
Catalytic activity	CTP + ethanolamine phosphate = diphosphate + CDP-ethanolamine.
Pathway	Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 2/3.
Tissue specificity	Strongest expression in liver, heart, and skeletal muscle.
Sequence similarities	Belongs to the cytidylyltransferase family.

Ontologies

Keywords
Biological process	Lipid biosynthesis Lipid metabolism Phospholipid biosynthesis Phospholipid metabolism
Coding sequence diversity	Alternative splicing
Domain	Repeat
Molecular function	Nucleotidyltransferase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	phosphatidylethanolamine biosynthetic process Traceable author statement. Source: Reactome small molecule metabolic process Traceable author statement. Source: Reactome
Cellular_component	endoplasmic reticulum membrane Traceable author statement. Source: Reactome
Molecular_function	ethanolamine-phosphate cytidylyltransferase activity Traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q99447-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q99447-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-78: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 389

389

Ethanolamine-phosphate cytidylyltransferase

PRO_0000208461

Regions

Nucleotide binding

221 – 222

2

CTP

Nucleotide binding

229 – 232

4

CTP

Nucleotide binding

307 – 310

4

CTP

Nucleotide binding

336 – 340

5

CTP

Sites

Binding site

259

1

CTP

Natural variations

Alternative sequence

1 – 78

78

Missing in isoform 2.

VSP_045131

Experimental info

Sequence conflict

244

1

Y → H in AAH10075. Ref.6

Secondary structure

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389

Helix Strand Turn

Details...

Beta strand

24 – 29

6

Helix

36 – 47

12

Beta strand

49 – 56

8

Helix

59 – 65

7

Helix

73 – 82

10

Beta strand

88 – 91

4

Helix

99 – 104

6

Beta strand

108 – 112

5

Helix

127 – 131

5

Beta strand

135 – 137

3

Helix

146 – 154

9

Helix

197 – 203

7

Beta strand

214 – 220

7

Helix

227 – 237

11

Beta strand

240 – 250

11

Helix

252 – 259

8

Helix

268 – 276

9

Beta strand

283 – 288

6

Helix

294 – 299

6

Beta strand

303 – 307

5

Helix

323 – 328

6

Beta strand

331 – 333

3

Helix

342 – 350

9

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 13FE8EAEB7FFAF7F	FASTA	389	43,835
	10 20 30 40 50 60 MIRNGRGAAG GAEQPGPGGR RAVRVWCDGC YDMVHYGHSN QLRQARAMGD YLIVGVHTDE 70 80 90 100 110 120 EIAKHKGPPV FTQEERYKMV QAIKWVDEVV PAAPYVTTLE TLDKYNCDFC VHGNDITLTV 130 140 150 160 170 180 DGRDTYEEVK QAGRYRECKR TQGVSTTDLV GRMLLVTKAH HSSQEMSSEY REYADSFGKC 190 200 210 220 230 240 PGGRNPWTGV SQFLQTSQKI IQFASGKEPQ PGETVIYVAG AFDLFHIGHV DFLEKVHRLA 250 260 270 280 290 300 ERPYIIAGLH FDQEVNHYKG KNYPIMNLHE RTLSVLACRY VSEVVIGAPY AVTAELLSHF 310 320 330 340 350 360 KVDLVCHGKT EIIPDRDGSD PYQEPKRRGI FRQIDSGSNL TTDLIVQRII TNRLEYEARN 370 380 QKKEAKELAF LEAARQQAAQ PLGERDGDF
	Isoform 2 [UniParc]. Checksum: AE4851C05A3B403C	FASTA	311	35,200
	10 20 30 40 50 60 MVQAIKWVDE VVPAAPYVTT LETLDKYNCD FCVHGNDITL TVDGRDTYEE VKQAGRYREC 70 80 90 100 110 120 KRTQGVSTTD LVGRMLLVTK AHHSSQEMSS EYREYADSFG KCPGGRNPWT GVSQFLQTSQ 130 140 150 160 170 180 KIIQFASGKE PQPGETVIYV AGAFDLFHIG HVDFLEKVHR LAERPYIIAG LHFDQEVNHY 190 200 210 220 230 240 KGKNYPIMNL HERTLSVLAC RYVSEVVIGA PYAVTAELLS HFKVDLVCHG KTEIIPDRDG 250 260 270 280 290 300 SDPYQEPKRR GIFRQIDSGS NLTTDLIVQR IITNRLEYEA RNQKKEAKEL AFLEAARQQA 310 AQPLGERDGD F

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a human cDNA for CTP-phosphoethanolamine cytidylyltransferase by complementation in vivo of a yeast mutant." Nakashima A., Hosaka K., Nikawa J. J. Biol. Chem. 272:9567-9572(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Testis.
[5]	"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C. Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Muscle.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]	"Human CTP:phosphoethanolamine cytidylyltransferase." Structural genomics consortium (SGC) Submitted (JUL-2011) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 18-356 IN COMPLEX WITH CYTIDINE-5'-MONOPHOSPHATE.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D84307 mRNA. Translation: BAA12311.1.
CR456779 mRNA. Translation: CAG33060.1.
AK301723 mRNA. Translation: BAH13541.1.
AC145207 Genomic DNA. No translation available.
CH471099 Genomic DNA. Translation: EAW89705.1.
BC000351 mRNA. Translation: AAH00351.1.
BC010075 mRNA. Translation: AAH10075.1.

IPI

IPI00015285.

RefSeq

NP_001243364.1. NM_001256435.1.
NP_002852.1. NM_002861.3.

UniGene

Hs.569843.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3ELB	X-ray	2.00	A	18-356	[»]

ProteinModelPortal

Q99447.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q99447. 1 interaction.

MINT

MINT-3059003.

STRING

9606.ENSP00000331719.

PTM databases

PhosphoSite

Q99447.

Polymorphism databases

DMDM

12585314.

2D gel databases

UCD-2DPAGE

Q99447.

Proteomic databases

PaxDb

Q99447.

PRIDE

Q99447.

Protocols and materials databases

DNASU

5833.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000331285; ENSP00000331719; ENSG00000185813.
ENST00000538936; ENSP00000439245; ENSG00000185813.
ENST00000570388; ENSP00000458330; ENSG00000185813.

GeneID

5833.

KEGG

hsa:5833.

UCSC

uc002kce.2. human.

Organism-specific databases

CTD

5833.

GeneCards

GC17M079862.

HGNC

HGNC:8756. PCYT2.

HPA

HPA023033.
HPA023034.

MIM

602679. gene.

neXtProt

NX_Q99447.

PharmGKB

PA33101.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG0615.

HOGENOM

HOG000187618.

HOVERGEN

HBG000865.

InParanoid

Q99447.

KO

K00967.

OrthoDB

EOG4NGGMV.

PhylomeDB

Q99447.

Enzyme and pathway databases

Reactome

REACT_111217. Metabolism.

UniPathway

UPA00558; UER00742.

Gene expression databases

ArrayExpress

Q99447.

Bgee

Q99447.

CleanEx

HS_PCYT2.

Genevestigator

Q99447.

GermOnline

ENSG00000185813. Homo sapiens.

Family and domain databases

Gene3D

3.40.50.620. 2 hits.

InterPro

IPR004821. Cyt_trans-like.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]

Pfam

PF01467. CTP_transf_2. 2 hits.
[Graphical view]

TIGRFAMs

TIGR00125. cyt_tran_rel. 2 hits.

ProtoNet

Search...

Other

EvolutionaryTrace

Q99447.

GenomeRNAi

5833.

NextBio

22732.

SOURCE

Search...

Entry information

Entry name

PCY2_HUMAN

Accession

Primary (citable) accession number: Q99447
Secondary accession number(s): B7Z7A5, Q6IBM3, Q96G08

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2001
Last sequence update:	May 1, 1997
Last modified:	May 1, 2013
This is version 123 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.