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Q99447 (PCY2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 132. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ethanolamine-phosphate cytidylyltransferase

EC=2.7.7.14
Alternative name(s):
CTP:phosphoethanolamine cytidylyltransferase
Phosphorylethanolamine transferase
Gene names
Name:PCYT2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length389 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays an important role in the biosynthesis of the phospholipid phosphatidylethanolamine. Catalyzes the formation of CDP-ethanolamine.

Catalytic activity

CTP + ethanolamine phosphate = diphosphate + CDP-ethanolamine.

Pathway

Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 2/3.

Tissue specificity

Strongest expression in liver, heart, and skeletal muscle.

Sequence similarities

Belongs to the cytidylyltransferase family.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q99447-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q99447-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-78: Missing.
Isoform 3 (identifier: Q99447-3)

The sequence of this isoform differs from the canonical sequence as follows:
     179-179: K → KPPHPIPAGDILSSEGCSQ
Note: No experimental confirmation available. Gene prediction based on cDNA data.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 389389Ethanolamine-phosphate cytidylyltransferase
PRO_0000208461

Regions

Nucleotide binding221 – 2222CTP
Nucleotide binding229 – 2324CTP
Nucleotide binding307 – 3104CTP
Nucleotide binding336 – 3405CTP

Sites

Binding site2591CTP

Natural variations

Alternative sequence1 – 7878Missing in isoform 2.
VSP_045131
Alternative sequence1791K → KPPHPIPAGDILSSEGCSQ in isoform 3.
VSP_046844

Experimental info

Sequence conflict2441Y → H in AAH10075. Ref.6

Secondary structure

............................................... 389
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 13FE8EAEB7FFAF7F

FASTA38943,835
        10         20         30         40         50         60 
MIRNGRGAAG GAEQPGPGGR RAVRVWCDGC YDMVHYGHSN QLRQARAMGD YLIVGVHTDE 

        70         80         90        100        110        120 
EIAKHKGPPV FTQEERYKMV QAIKWVDEVV PAAPYVTTLE TLDKYNCDFC VHGNDITLTV 

       130        140        150        160        170        180 
DGRDTYEEVK QAGRYRECKR TQGVSTTDLV GRMLLVTKAH HSSQEMSSEY REYADSFGKC 

       190        200        210        220        230        240 
PGGRNPWTGV SQFLQTSQKI IQFASGKEPQ PGETVIYVAG AFDLFHIGHV DFLEKVHRLA 

       250        260        270        280        290        300 
ERPYIIAGLH FDQEVNHYKG KNYPIMNLHE RTLSVLACRY VSEVVIGAPY AVTAELLSHF 

       310        320        330        340        350        360 
KVDLVCHGKT EIIPDRDGSD PYQEPKRRGI FRQIDSGSNL TTDLIVQRII TNRLEYEARN 

       370        380 
QKKEAKELAF LEAARQQAAQ PLGERDGDF 

« Hide

Isoform 2 [UniParc].

Checksum: AE4851C05A3B403C
Show »

FASTA31135,200
Isoform 3 [UniParc].

Checksum: 8BA20CC46F0F06C9
Show »

FASTA40745,622

References

« Hide 'large scale' references
[1]"Cloning of a human cDNA for CTP-phosphoethanolamine cytidylyltransferase by complementation in vivo of a yeast mutant."
Nakashima A., Hosaka K., Nikawa J.
J. Biol. Chem. 272:9567-9572(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Testis.
[5]"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. expand/collapse author list , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Muscle.
[7]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Human CTP:phosphoethanolamine cytidylyltransferase."
Structural genomics consortium (SGC)
Submitted (JUL-2011) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 18-356 IN COMPLEX WITH CYTIDINE-5'-MONOPHOSPHATE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D84307 mRNA. Translation: BAA12311.1.
CR456779 mRNA. Translation: CAG33060.1.
AK301723 mRNA. Translation: BAH13541.1.
AC145207 Genomic DNA. No translation available.
CH471099 Genomic DNA. Translation: EAW89705.1.
BC000351 mRNA. Translation: AAH00351.1.
BC010075 mRNA. Translation: AAH10075.1.
RefSeqNP_001171846.1. NM_001184917.2.
NP_001243364.1. NM_001256435.2.
NP_001269132.1. NM_001282203.1.
NP_002852.1. NM_002861.4.
UniGeneHs.569843.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3ELBX-ray2.00A18-356[»]
ProteinModelPortalQ99447.
SMRQ99447. Positions 19-351.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid111791. 17 interactions.
IntActQ99447. 7 interactions.
MINTMINT-3059003.
STRING9606.ENSP00000331719.

PTM databases

PhosphoSiteQ99447.

Polymorphism databases

DMDM12585314.

2D gel databases

UCD-2DPAGEQ99447.

Proteomic databases

PaxDbQ99447.
PRIDEQ99447.

Protocols and materials databases

DNASU5833.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000331285; ENSP00000331719; ENSG00000185813. [Q99447-2]
ENST00000538721; ENSP00000442050; ENSG00000185813. [Q99447-3]
ENST00000538936; ENSP00000439245; ENSG00000185813. [Q99447-1]
ENST00000570388; ENSP00000458330; ENSG00000185813. [Q99447-2]
GeneID5833.
KEGGhsa:5833.
UCSCuc002kce.2. human. [Q99447-1]

Organism-specific databases

CTD5833.
GeneCardsGC17M079862.
HGNCHGNC:8756. PCYT2.
HPAHPA023033.
HPA023034.
MIM602679. gene.
neXtProtNX_Q99447.
PharmGKBPA33101.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0615.
HOGENOMHOG000187618.
HOVERGENHBG000865.
InParanoidQ99447.
KOK00967.
OMAADSFGKP.
OrthoDBEOG7CRTPS.
PhylomeDBQ99447.
TreeFamTF106337.

Enzyme and pathway databases

ReactomeREACT_111217. Metabolism.
UniPathwayUPA00558; UER00742.

Gene expression databases

ArrayExpressQ99447.
BgeeQ99447.
CleanExHS_PCYT2.
GenevestigatorQ99447.

Family and domain databases

Gene3D3.40.50.620. 2 hits.
InterProIPR004821. Cyt_trans-like.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF01467. CTP_transf_2. 2 hits.
[Graphical view]
TIGRFAMsTIGR00125. cyt_tran_rel. 2 hits.
ProtoNetSearch...

Other

EvolutionaryTraceQ99447.
GenomeRNAi5833.
NextBio22732.
PROQ99447.
SOURCESearch...

Entry information

Entry namePCY2_HUMAN
AccessionPrimary (citable) accession number: Q99447
Secondary accession number(s): B7Z7A5 expand/collapse secondary AC list , F5H8B1, Q6IBM3, Q96G08
Entry history
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: May 1, 1997
Last modified: April 16, 2014
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM