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Protein

Ethanolamine-phosphate cytidylyltransferase

Gene

PCYT2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in the biosynthesis of the phospholipid phosphatidylethanolamine. Catalyzes the formation of CDP-ethanolamine.

Catalytic activityi

CTP + ethanolamine phosphate = diphosphate + CDP-ethanolamine.

Pathwayi: phosphatidylethanolamine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes phosphatidylethanolamine from ethanolamine.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Choline/ethanolamine kinase (CHKB), Ethanolamine kinase 2 (ETNK2), Choline kinase alpha (CHKA), Ethanolamine kinase 1 (ETNK1)
  2. Ethanolamine-phosphate cytidylyltransferase (PCYT2)
  3. Ethanolaminephosphotransferase 1 (EPT1), Choline/ethanolaminephosphotransferase 1 (CEPT1)
This subpathway is part of the pathway phosphatidylethanolamine biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylethanolamine from ethanolamine, the pathway phosphatidylethanolamine biosynthesis and in Phospholipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei259CTP1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi221 – 222CTP2
Nucleotide bindingi229 – 232CTP4
Nucleotide bindingi307 – 310CTP4
Nucleotide bindingi336 – 340CTP5

GO - Molecular functioni

  • ethanolamine-phosphate cytidylyltransferase activity Source: UniProtKB

GO - Biological processi

  • phosphatidylethanolamine biosynthetic process Source: Reactome
  • phospholipid biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:HS06840-MONOMER.
ZFISH:HS06840-MONOMER.
ReactomeiR-HSA-1483213. Synthesis of PE.
UniPathwayiUPA00558; UER00742.

Names & Taxonomyi

Protein namesi
Recommended name:
Ethanolamine-phosphate cytidylyltransferase (EC:2.7.7.14)
Alternative name(s):
CTP:phosphoethanolamine cytidylyltransferase
Phosphorylethanolamine transferase
Gene namesi
Name:PCYT2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:8756. PCYT2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000185813.
PharmGKBiPA33101.

Chemistry databases

DrugBankiDB00709. Lamivudine.

Polymorphism and mutation databases

DMDMi12585314.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002084611 – 389Ethanolamine-phosphate cytidylyltransferaseAdd BLAST389

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei338PhosphoserineCombined sources1
Modified residuei341PhosphothreonineCombined sources1
Modified residuei342PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ99447.
MaxQBiQ99447.
PaxDbiQ99447.
PeptideAtlasiQ99447.
PRIDEiQ99447.

2D gel databases

UCD-2DPAGEQ99447.

PTM databases

iPTMnetiQ99447.
PhosphoSitePlusiQ99447.

Expressioni

Tissue specificityi

Strongest expression in liver, heart, and skeletal muscle.

Gene expression databases

BgeeiENSG00000185813.
CleanExiHS_PCYT2.
ExpressionAtlasiQ99447. baseline and differential.
GenevisibleiQ99447. HS.

Organism-specific databases

HPAiHPA023033.
HPA023034.

Interactioni

Protein-protein interaction databases

BioGridi111791. 19 interactors.
IntActiQ99447. 7 interactors.
MINTiMINT-3059003.
STRINGi9606.ENSP00000442050.

Structurei

Secondary structure

1389
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi24 – 29Combined sources6
Helixi36 – 47Combined sources12
Beta strandi49 – 56Combined sources8
Helixi59 – 65Combined sources7
Helixi73 – 82Combined sources10
Beta strandi88 – 91Combined sources4
Helixi99 – 104Combined sources6
Beta strandi108 – 112Combined sources5
Helixi127 – 131Combined sources5
Beta strandi135 – 137Combined sources3
Helixi146 – 154Combined sources9
Helixi197 – 203Combined sources7
Beta strandi214 – 220Combined sources7
Helixi227 – 237Combined sources11
Beta strandi240 – 250Combined sources11
Helixi252 – 259Combined sources8
Helixi268 – 276Combined sources9
Beta strandi283 – 288Combined sources6
Helixi294 – 299Combined sources6
Beta strandi303 – 307Combined sources5
Helixi323 – 328Combined sources6
Beta strandi331 – 333Combined sources3
Helixi342 – 350Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3ELBX-ray2.00A18-356[»]
4XSVX-ray2.70A18-356[»]
ProteinModelPortaliQ99447.
SMRiQ99447.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ99447.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytidylyltransferase family.Curated

Phylogenomic databases

eggNOGiKOG2803. Eukaryota.
COG0615. LUCA.
GeneTreeiENSGT00550000075065.
HOGENOMiHOG000187618.
HOVERGENiHBG000865.
InParanoidiQ99447.
KOiK00967.
OMAiNDMTTEK.
OrthoDBiEOG091G0AIL.
PhylomeDBiQ99447.
TreeFamiTF106337.

Family and domain databases

Gene3Di3.40.50.620. 2 hits.
InterProiIPR004821. Cyt_trans-like.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01467. CTP_transf_like. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00125. cyt_tran_rel. 2 hits.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q99447-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIRNGRGAAG GAEQPGPGGR RAVRVWCDGC YDMVHYGHSN QLRQARAMGD
60 70 80 90 100
YLIVGVHTDE EIAKHKGPPV FTQEERYKMV QAIKWVDEVV PAAPYVTTLE
110 120 130 140 150
TLDKYNCDFC VHGNDITLTV DGRDTYEEVK QAGRYRECKR TQGVSTTDLV
160 170 180 190 200
GRMLLVTKAH HSSQEMSSEY REYADSFGKC PGGRNPWTGV SQFLQTSQKI
210 220 230 240 250
IQFASGKEPQ PGETVIYVAG AFDLFHIGHV DFLEKVHRLA ERPYIIAGLH
260 270 280 290 300
FDQEVNHYKG KNYPIMNLHE RTLSVLACRY VSEVVIGAPY AVTAELLSHF
310 320 330 340 350
KVDLVCHGKT EIIPDRDGSD PYQEPKRRGI FRQIDSGSNL TTDLIVQRII
360 370 380
TNRLEYEARN QKKEAKELAF LEAARQQAAQ PLGERDGDF
Length:389
Mass (Da):43,835
Last modified:May 1, 1997 - v1
Checksum:i13FE8EAEB7FFAF7F
GO
Isoform 2 (identifier: Q99447-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-78: Missing.

Show »
Length:311
Mass (Da):35,200
Checksum:iAE4851C05A3B403C
GO
Isoform 3 (identifier: Q99447-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     179-179: K → KPPHPIPAGDILSSEGCSQ

Note: No experimental confirmation available. Gene prediction based on cDNA data.
Show »
Length:407
Mass (Da):45,622
Checksum:i8BA20CC46F0F06C9
GO
Isoform 4 (identifier: Q99447-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-32: Missing.

Note: No experimental confirmation available.
Show »
Length:357
Mass (Da):40,520
Checksum:iF246E5C6A87F55CD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti244Y → H in AAH10075 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0451311 – 78Missing in isoform 2. 1 PublicationAdd BLAST78
Alternative sequenceiVSP_0546151 – 32Missing in isoform 4. 1 PublicationAdd BLAST32
Alternative sequenceiVSP_046844179K → KPPHPIPAGDILSSEGCSQ in isoform 3. Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84307 mRNA. Translation: BAA12311.1.
CR456779 mRNA. Translation: CAG33060.1.
AK301723 mRNA. Translation: BAH13541.1.
AK316385 mRNA. Translation: BAH14756.1.
AC145207 Genomic DNA. No translation available.
CH471099 Genomic DNA. Translation: EAW89705.1.
BC000351 mRNA. Translation: AAH00351.1.
BC010075 mRNA. Translation: AAH10075.1.
CCDSiCCDS11791.1. [Q99447-1]
CCDS54178.1. [Q99447-3]
CCDS58610.1. [Q99447-2]
CCDS62364.1. [Q99447-4]
RefSeqiNP_001171846.1. NM_001184917.2. [Q99447-3]
NP_001243364.1. NM_001256435.2. [Q99447-2]
NP_001269132.1. NM_001282203.1. [Q99447-2]
NP_001269133.1. NM_001282204.1. [Q99447-4]
NP_002852.1. NM_002861.4. [Q99447-1]
XP_016880400.1. XM_017024911.1. [Q99447-4]
UniGeneiHs.569843.

Genome annotation databases

EnsembliENST00000331285; ENSP00000331719; ENSG00000185813. [Q99447-2]
ENST00000538721; ENSP00000442050; ENSG00000185813. [Q99447-3]
ENST00000538936; ENSP00000439245; ENSG00000185813. [Q99447-1]
ENST00000570388; ENSP00000458330; ENSG00000185813. [Q99447-2]
ENST00000570391; ENSP00000461190; ENSG00000185813. [Q99447-4]
GeneIDi5833.
KEGGihsa:5833.
UCSCiuc002kce.4. human. [Q99447-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84307 mRNA. Translation: BAA12311.1.
CR456779 mRNA. Translation: CAG33060.1.
AK301723 mRNA. Translation: BAH13541.1.
AK316385 mRNA. Translation: BAH14756.1.
AC145207 Genomic DNA. No translation available.
CH471099 Genomic DNA. Translation: EAW89705.1.
BC000351 mRNA. Translation: AAH00351.1.
BC010075 mRNA. Translation: AAH10075.1.
CCDSiCCDS11791.1. [Q99447-1]
CCDS54178.1. [Q99447-3]
CCDS58610.1. [Q99447-2]
CCDS62364.1. [Q99447-4]
RefSeqiNP_001171846.1. NM_001184917.2. [Q99447-3]
NP_001243364.1. NM_001256435.2. [Q99447-2]
NP_001269132.1. NM_001282203.1. [Q99447-2]
NP_001269133.1. NM_001282204.1. [Q99447-4]
NP_002852.1. NM_002861.4. [Q99447-1]
XP_016880400.1. XM_017024911.1. [Q99447-4]
UniGeneiHs.569843.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3ELBX-ray2.00A18-356[»]
4XSVX-ray2.70A18-356[»]
ProteinModelPortaliQ99447.
SMRiQ99447.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111791. 19 interactors.
IntActiQ99447. 7 interactors.
MINTiMINT-3059003.
STRINGi9606.ENSP00000442050.

Chemistry databases

DrugBankiDB00709. Lamivudine.

PTM databases

iPTMnetiQ99447.
PhosphoSitePlusiQ99447.

Polymorphism and mutation databases

DMDMi12585314.

2D gel databases

UCD-2DPAGEQ99447.

Proteomic databases

EPDiQ99447.
MaxQBiQ99447.
PaxDbiQ99447.
PeptideAtlasiQ99447.
PRIDEiQ99447.

Protocols and materials databases

DNASUi5833.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000331285; ENSP00000331719; ENSG00000185813. [Q99447-2]
ENST00000538721; ENSP00000442050; ENSG00000185813. [Q99447-3]
ENST00000538936; ENSP00000439245; ENSG00000185813. [Q99447-1]
ENST00000570388; ENSP00000458330; ENSG00000185813. [Q99447-2]
ENST00000570391; ENSP00000461190; ENSG00000185813. [Q99447-4]
GeneIDi5833.
KEGGihsa:5833.
UCSCiuc002kce.4. human. [Q99447-1]

Organism-specific databases

CTDi5833.
GeneCardsiPCYT2.
HGNCiHGNC:8756. PCYT2.
HPAiHPA023033.
HPA023034.
MIMi602679. gene.
neXtProtiNX_Q99447.
OpenTargetsiENSG00000185813.
PharmGKBiPA33101.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2803. Eukaryota.
COG0615. LUCA.
GeneTreeiENSGT00550000075065.
HOGENOMiHOG000187618.
HOVERGENiHBG000865.
InParanoidiQ99447.
KOiK00967.
OMAiNDMTTEK.
OrthoDBiEOG091G0AIL.
PhylomeDBiQ99447.
TreeFamiTF106337.

Enzyme and pathway databases

UniPathwayiUPA00558; UER00742.
BioCyciMetaCyc:HS06840-MONOMER.
ZFISH:HS06840-MONOMER.
ReactomeiR-HSA-1483213. Synthesis of PE.

Miscellaneous databases

EvolutionaryTraceiQ99447.
GenomeRNAii5833.
PROiQ99447.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000185813.
CleanExiHS_PCYT2.
ExpressionAtlasiQ99447. baseline and differential.
GenevisibleiQ99447. HS.

Family and domain databases

Gene3Di3.40.50.620. 2 hits.
InterProiIPR004821. Cyt_trans-like.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01467. CTP_transf_like. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00125. cyt_tran_rel. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiPCY2_HUMAN
AccessioniPrimary (citable) accession number: Q99447
Secondary accession number(s): B7Z7A5
, B7ZAS0, F5H8B1, Q6IBM3, Q96G08
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: May 1, 1997
Last modified: November 2, 2016
This is version 157 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.