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Q99447

- PCY2_HUMAN

UniProt

Q99447 - PCY2_HUMAN

Protein

Ethanolamine-phosphate cytidylyltransferase

Gene

PCYT2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 137 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
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    Functioni

    Plays an important role in the biosynthesis of the phospholipid phosphatidylethanolamine. Catalyzes the formation of CDP-ethanolamine.

    Catalytic activityi

    CTP + ethanolamine phosphate = diphosphate + CDP-ethanolamine.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei259 – 2591CTP

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi221 – 2222CTP
    Nucleotide bindingi229 – 2324CTP
    Nucleotide bindingi307 – 3104CTP
    Nucleotide bindingi336 – 3405CTP

    GO - Molecular functioni

    1. ethanolamine-phosphate cytidylyltransferase activity Source: UniProtKB

    GO - Biological processi

    1. glycerophospholipid biosynthetic process Source: Reactome
    2. phosphatidylethanolamine biosynthetic process Source: Reactome
    3. phospholipid biosynthetic process Source: UniProtKB
    4. phospholipid metabolic process Source: Reactome
    5. small molecule metabolic process Source: Reactome

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

    Enzyme and pathway databases

    ReactomeiREACT_120919. Synthesis of PE.
    UniPathwayiUPA00558; UER00742.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ethanolamine-phosphate cytidylyltransferase (EC:2.7.7.14)
    Alternative name(s):
    CTP:phosphoethanolamine cytidylyltransferase
    Phosphorylethanolamine transferase
    Gene namesi
    Name:PCYT2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 17

    Organism-specific databases

    HGNCiHGNC:8756. PCYT2.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: Reactome

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA33101.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 389389Ethanolamine-phosphate cytidylyltransferasePRO_0000208461Add
    BLAST

    Proteomic databases

    MaxQBiQ99447.
    PaxDbiQ99447.
    PRIDEiQ99447.

    2D gel databases

    UCD-2DPAGEQ99447.

    PTM databases

    PhosphoSiteiQ99447.

    Expressioni

    Tissue specificityi

    Strongest expression in liver, heart, and skeletal muscle.

    Gene expression databases

    ArrayExpressiQ99447.
    BgeeiQ99447.
    CleanExiHS_PCYT2.
    GenevestigatoriQ99447.

    Organism-specific databases

    HPAiHPA023033.
    HPA023034.

    Interactioni

    Protein-protein interaction databases

    BioGridi111791. 19 interactions.
    IntActiQ99447. 7 interactions.
    MINTiMINT-3059003.
    STRINGi9606.ENSP00000331719.

    Structurei

    Secondary structure

    1
    389
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi24 – 296
    Helixi36 – 4712
    Beta strandi49 – 568
    Helixi59 – 657
    Helixi73 – 8210
    Beta strandi88 – 914
    Helixi99 – 1046
    Beta strandi108 – 1125
    Helixi127 – 1315
    Beta strandi135 – 1373
    Helixi146 – 1549
    Helixi197 – 2037
    Beta strandi214 – 2207
    Helixi227 – 23711
    Beta strandi240 – 25011
    Helixi252 – 2598
    Helixi268 – 2769
    Beta strandi283 – 2886
    Helixi294 – 2996
    Beta strandi303 – 3075
    Helixi323 – 3286
    Beta strandi331 – 3333
    Helixi342 – 3509

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3ELBX-ray2.00A18-356[»]
    ProteinModelPortaliQ99447.
    SMRiQ99447. Positions 19-351.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ99447.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytidylyltransferase family.Curated

    Phylogenomic databases

    eggNOGiCOG0615.
    HOGENOMiHOG000187618.
    HOVERGENiHBG000865.
    InParanoidiQ99447.
    KOiK00967.
    OMAiADSFGKP.
    OrthoDBiEOG7CRTPS.
    PhylomeDBiQ99447.
    TreeFamiTF106337.

    Family and domain databases

    Gene3Di3.40.50.620. 2 hits.
    InterProiIPR004821. Cyt_trans-like.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PfamiPF01467. CTP_transf_2. 2 hits.
    [Graphical view]
    TIGRFAMsiTIGR00125. cyt_tran_rel. 2 hits.

    Sequences (4)i

    Sequence statusi: Complete.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q99447-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MIRNGRGAAG GAEQPGPGGR RAVRVWCDGC YDMVHYGHSN QLRQARAMGD    50
    YLIVGVHTDE EIAKHKGPPV FTQEERYKMV QAIKWVDEVV PAAPYVTTLE 100
    TLDKYNCDFC VHGNDITLTV DGRDTYEEVK QAGRYRECKR TQGVSTTDLV 150
    GRMLLVTKAH HSSQEMSSEY REYADSFGKC PGGRNPWTGV SQFLQTSQKI 200
    IQFASGKEPQ PGETVIYVAG AFDLFHIGHV DFLEKVHRLA ERPYIIAGLH 250
    FDQEVNHYKG KNYPIMNLHE RTLSVLACRY VSEVVIGAPY AVTAELLSHF 300
    KVDLVCHGKT EIIPDRDGSD PYQEPKRRGI FRQIDSGSNL TTDLIVQRII 350
    TNRLEYEARN QKKEAKELAF LEAARQQAAQ PLGERDGDF 389
    Length:389
    Mass (Da):43,835
    Last modified:May 1, 1997 - v1
    Checksum:i13FE8EAEB7FFAF7F
    GO
    Isoform 2 (identifier: Q99447-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-78: Missing.

    Show »
    Length:311
    Mass (Da):35,200
    Checksum:iAE4851C05A3B403C
    GO
    Isoform 3 (identifier: Q99447-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         179-179: K → KPPHPIPAGDILSSEGCSQ

    Note: No experimental confirmation available. Gene prediction based on cDNA data.

    Show »
    Length:407
    Mass (Da):45,622
    Checksum:i8BA20CC46F0F06C9
    GO
    Isoform 4 (identifier: Q99447-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-32: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:357
    Mass (Da):40,520
    Checksum:iF246E5C6A87F55CD
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti244 – 2441Y → H in AAH10075. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 7878Missing in isoform 2. 1 PublicationVSP_045131Add
    BLAST
    Alternative sequencei1 – 3232Missing in isoform 4. 1 PublicationVSP_054615Add
    BLAST
    Alternative sequencei179 – 1791K → KPPHPIPAGDILSSEGCSQ in isoform 3. CuratedVSP_046844

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D84307 mRNA. Translation: BAA12311.1.
    CR456779 mRNA. Translation: CAG33060.1.
    AK301723 mRNA. Translation: BAH13541.1.
    AK316385 mRNA. Translation: BAH14756.1.
    AC145207 Genomic DNA. No translation available.
    CH471099 Genomic DNA. Translation: EAW89705.1.
    BC000351 mRNA. Translation: AAH00351.1.
    BC010075 mRNA. Translation: AAH10075.1.
    CCDSiCCDS11791.1. [Q99447-1]
    CCDS54178.1. [Q99447-3]
    CCDS58610.1. [Q99447-2]
    CCDS62364.1. [Q99447-4]
    RefSeqiNP_001171846.1. NM_001184917.2. [Q99447-3]
    NP_001243364.1. NM_001256435.2. [Q99447-2]
    NP_001269132.1. NM_001282203.1. [Q99447-2]
    NP_001269133.1. NM_001282204.1. [Q99447-4]
    NP_002852.1. NM_002861.4. [Q99447-1]
    UniGeneiHs.569843.

    Genome annotation databases

    EnsembliENST00000331285; ENSP00000331719; ENSG00000185813. [Q99447-2]
    ENST00000538721; ENSP00000442050; ENSG00000185813. [Q99447-3]
    ENST00000538936; ENSP00000439245; ENSG00000185813. [Q99447-1]
    ENST00000570388; ENSP00000458330; ENSG00000185813. [Q99447-2]
    ENST00000570391; ENSP00000461190; ENSG00000185813. [Q99447-4]
    GeneIDi5833.
    KEGGihsa:5833.
    UCSCiuc002kce.2. human. [Q99447-1]

    Polymorphism databases

    DMDMi12585314.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D84307 mRNA. Translation: BAA12311.1 .
    CR456779 mRNA. Translation: CAG33060.1 .
    AK301723 mRNA. Translation: BAH13541.1 .
    AK316385 mRNA. Translation: BAH14756.1 .
    AC145207 Genomic DNA. No translation available.
    CH471099 Genomic DNA. Translation: EAW89705.1 .
    BC000351 mRNA. Translation: AAH00351.1 .
    BC010075 mRNA. Translation: AAH10075.1 .
    CCDSi CCDS11791.1. [Q99447-1 ]
    CCDS54178.1. [Q99447-3 ]
    CCDS58610.1. [Q99447-2 ]
    CCDS62364.1. [Q99447-4 ]
    RefSeqi NP_001171846.1. NM_001184917.2. [Q99447-3 ]
    NP_001243364.1. NM_001256435.2. [Q99447-2 ]
    NP_001269132.1. NM_001282203.1. [Q99447-2 ]
    NP_001269133.1. NM_001282204.1. [Q99447-4 ]
    NP_002852.1. NM_002861.4. [Q99447-1 ]
    UniGenei Hs.569843.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3ELB X-ray 2.00 A 18-356 [» ]
    ProteinModelPortali Q99447.
    SMRi Q99447. Positions 19-351.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 111791. 19 interactions.
    IntActi Q99447. 7 interactions.
    MINTi MINT-3059003.
    STRINGi 9606.ENSP00000331719.

    PTM databases

    PhosphoSitei Q99447.

    Polymorphism databases

    DMDMi 12585314.

    2D gel databases

    UCD-2DPAGE Q99447.

    Proteomic databases

    MaxQBi Q99447.
    PaxDbi Q99447.
    PRIDEi Q99447.

    Protocols and materials databases

    DNASUi 5833.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000331285 ; ENSP00000331719 ; ENSG00000185813 . [Q99447-2 ]
    ENST00000538721 ; ENSP00000442050 ; ENSG00000185813 . [Q99447-3 ]
    ENST00000538936 ; ENSP00000439245 ; ENSG00000185813 . [Q99447-1 ]
    ENST00000570388 ; ENSP00000458330 ; ENSG00000185813 . [Q99447-2 ]
    ENST00000570391 ; ENSP00000461190 ; ENSG00000185813 . [Q99447-4 ]
    GeneIDi 5833.
    KEGGi hsa:5833.
    UCSCi uc002kce.2. human. [Q99447-1 ]

    Organism-specific databases

    CTDi 5833.
    GeneCardsi GC17M079862.
    HGNCi HGNC:8756. PCYT2.
    HPAi HPA023033.
    HPA023034.
    MIMi 602679. gene.
    neXtProti NX_Q99447.
    PharmGKBi PA33101.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0615.
    HOGENOMi HOG000187618.
    HOVERGENi HBG000865.
    InParanoidi Q99447.
    KOi K00967.
    OMAi ADSFGKP.
    OrthoDBi EOG7CRTPS.
    PhylomeDBi Q99447.
    TreeFami TF106337.

    Enzyme and pathway databases

    UniPathwayi UPA00558 ; UER00742 .
    Reactomei REACT_120919. Synthesis of PE.

    Miscellaneous databases

    EvolutionaryTracei Q99447.
    GenomeRNAii 5833.
    NextBioi 22732.
    PROi Q99447.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q99447.
    Bgeei Q99447.
    CleanExi HS_PCYT2.
    Genevestigatori Q99447.

    Family and domain databases

    Gene3Di 3.40.50.620. 2 hits.
    InterProi IPR004821. Cyt_trans-like.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    Pfami PF01467. CTP_transf_2. 2 hits.
    [Graphical view ]
    TIGRFAMsi TIGR00125. cyt_tran_rel. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of a human cDNA for CTP-phosphoethanolamine cytidylyltransferase by complementation in vivo of a yeast mutant."
      Nakashima A., Hosaka K., Nikawa J.
      J. Biol. Chem. 272:9567-9572(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 4).
      Tissue: Testis.
    5. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
      Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
      , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
      Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Muscle.
    7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Human CTP:phosphoethanolamine cytidylyltransferase."
      Structural genomics consortium (SGC)
      Submitted (JUL-2011) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 18-356 IN COMPLEX WITH CYTIDINE-5'-MONOPHOSPHATE.

    Entry informationi

    Entry nameiPCY2_HUMAN
    AccessioniPrimary (citable) accession number: Q99447
    Secondary accession number(s): B7Z7A5
    , B7ZAS0, F5H8B1, Q6IBM3, Q96G08
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 24, 2001
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 137 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 17
      Human chromosome 17: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3