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Q99439 (CNN2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 136. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Calponin-2
Alternative name(s):
Calponin H2, smooth muscle
Neutral calponin
Gene names
Name:CNN2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length309 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.

Tissue specificity

Heart and smooth muscle.

Sequence similarities

Belongs to the calponin family.

Contains 3 calponin-like repeats.

Contains 1 CH (calponin-homology) domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q99439-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q99439-2)

The sequence of this isoform differs from the canonical sequence as follows:
     131-169: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.7 Ref.8
Chain2 – 309308Calponin-2
PRO_0000204773

Regions

Domain28 – 131104CH
Repeat166 – 19126Calponin-like 1
Repeat206 – 23126Calponin-like 2
Repeat245 – 26925Calponin-like 3

Amino acid modifications

Modified residue21N-acetylserine Ref.8 Ref.10
Modified residue81N6-acetyllysine Ref.8
Modified residue251N6-acetyllysine Ref.8

Natural variations

Alternative sequence131 – 16939Missing in isoform 2.
VSP_042941

Experimental info

Sequence conflict521K → R in BAA20887. Ref.6
Sequence conflict1421I → V in BAA20887. Ref.6
Sequence conflict1651V → G in BAA20887. Ref.6
Sequence conflict182 – 1832TA → HL in BAA20887. Ref.6
Sequence conflict187 – 20216RRHLY…LPPMD → AGISMTPRTISCPPWT in BAA20887. Ref.6

Secondary structure

................ 309
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: 0D767E9040190A5F

FASTA30933,697
        10         20         30         40         50         60 
MSSTQFNKGP SYGLSAEVKN RLLSKYDPQK EAELRTWIEG LTGLSIGPDF QKGLKDGTIL 

        70         80         90        100        110        120 
CTLMNKLQPG SVPKINRSMQ NWHQLENLSN FIKAMVSYGM NPVDLFEAND LFESGNMTQV 

       130        140        150        160        170        180 
QVSLLALAGK AKTKGLQSGV DIGVKYSEKQ ERNFDDATMK AGQCVIGLQM GTNKCASQSG 

       190        200        210        220        230        240 
MTAYGTRRHL YDPKNHILPP MDHSTISLQM GTNKCASQVG MTAPGTRRHI YDTKLGTDKC 

       250        260        270        280        290        300 
DNSSMSLQMG YTQGANQSGQ VFGLGRQIYD PKYCPQGTVA DGAPSGTGDC PDPGEVPEYP 


PYYQEEAGY 

« Hide

Isoform 2 [UniParc].

Checksum: 6CCEFDE1EE200798
Show »

FASTA27029,500

References

« Hide 'large scale' references
[1]"Molecular cloning and characterization of human non-smooth muscle calponin."
Masuda H., Tanaka K., Takagi M., Ohgami K., Sakamaki T., Shibata N., Takahashi K.
J. Biochem. 120:415-424(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Heart.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Uterus.
[3]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]"A unique downregulation of h2-calponin gene expression in Down syndrome: a possible attenuation mechanism for fetal survival by methylation at the CpG island in the trisomic chromosome 21."
Kuromitsu J., Yamashita H., Kataoka H., Takahara T., Muramatsu M., Sekine T., Okamoto N., Furuichi Y., Hayashizaki Y.
Mol. Cell. Biol. 17:707-712(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-202 (ISOFORM 1).
Tissue: Placenta.
[7]"Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."
Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J.
Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-21.
Tissue: Platelet.
[8]"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2; LYS-8 AND LYS-25, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
[9]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Solution structure of the CH domain of human calponin-2."
RIKEN structural genomics initiative (RSGI)
Submitted (AUG-2005) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 20-152.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D83735 mRNA. Translation: BAA12090.1.
AK126391 mRNA. Translation: BAG54322.1.
AC004528 Genomic DNA. No translation available.
AC011558 Genomic DNA. No translation available.
CH471139 Genomic DNA. Translation: EAW69565.1.
CH471139 Genomic DNA. Translation: EAW69567.1.
CH471139 Genomic DNA. Translation: EAW69570.1.
BC141818 mRNA. Translation: AAI41819.1.
BC141833 mRNA. Translation: AAI41834.1.
D86059 mRNA. Translation: BAA20887.1.
PIRJC4906.
RefSeqNP_004359.1. NM_004368.2.
NP_958434.1. NM_201277.1.
UniGeneHs.651512.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1WYNNMR-A20-151[»]
ProteinModelPortalQ99439.
SMRQ99439. Positions 13-153.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid107665. 15 interactions.
IntActQ99439. 1 interaction.
STRING9606.ENSP00000263097.

PTM databases

PhosphoSiteQ99439.

Polymorphism databases

DMDM6226844.

2D gel databases

OGPQ99439.

Proteomic databases

PaxDbQ99439.
PeptideAtlasQ99439.
PRIDEQ99439.

Protocols and materials databases

DNASU1265.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000263097; ENSP00000263097; ENSG00000064666. [Q99439-1]
ENST00000348419; ENSP00000340129; ENSG00000064666. [Q99439-2]
GeneID1265.
KEGGhsa:1265.
UCSCuc002lqu.3. human. [Q99439-1]
uc002lqv.3. human. [Q99439-2]

Organism-specific databases

CTD1265.
GeneCardsGC19P001026.
HGNCHGNC:2156. CNN2.
HPAHPA049095.
MIM602373. gene.
neXtProtNX_Q99439.
PharmGKBPA26666.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5199.
HOGENOMHOG000232113.
HOVERGENHBG005186.
InParanoidQ99439.
OrthoDBEOG71P2B3.
PhylomeDBQ99439.
TreeFamTF313921.

Gene expression databases

ArrayExpressQ99439.
BgeeQ99439.
CleanExHS_CNN2.
GenevestigatorQ99439.

Family and domain databases

Gene3D1.10.418.10. 1 hit.
InterProIPR001997. Calponin.
IPR000557. Calponin_repeat.
IPR001715. CH-domain.
IPR003096. SM22_calponin.
[Graphical view]
PfamPF00402. Calponin. 3 hits.
PF00307. CH. 1 hit.
[Graphical view]
PRINTSPR00889. CALPONIN.
PR00888. SM22CALPONIN.
SMARTSM00033. CH. 1 hit.
[Graphical view]
SUPFAMSSF47576. SSF47576. 1 hit.
PROSITEPS01052. CALPONIN_1. 3 hits.
PS51122. CALPONIN_2. 3 hits.
PS50021. CH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSCNN2. human.
EvolutionaryTraceQ99439.
GenomeRNAi1265.
NextBio5119.
PROQ99439.
SOURCESearch...

Entry information

Entry nameCNN2_HUMAN
AccessionPrimary (citable) accession number: Q99439
Secondary accession number(s): A5D8U8 expand/collapse secondary AC list , A6NFI4, D6W5X9, Q92578
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 136 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM