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Q99439

- CNN2_HUMAN

UniProt

Q99439 - CNN2_HUMAN

Protein

Calponin-2

Gene

CNN2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
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    • History
      Entry version 140 (01 Oct 2014)
      Sequence version 4 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.

    GO - Molecular functioni

    1. actin binding Source: ProtInc

    GO - Biological processi

    1. actomyosin structure organization Source: InterPro
    2. cellular response to mechanical stimulus Source: MGI
    3. cytoskeleton organization Source: ProtInc
    4. hemopoiesis Source: Ensembl
    5. negative regulation of cell migration Source: Ensembl
    6. negative regulation of phagocytosis Source: Ensembl
    7. positive regulation of gene expression Source: Ensembl
    8. regulation of actin filament-based process Source: MGI
    9. regulation of cell proliferation Source: Ensembl
    10. wound healing Source: Ensembl

    Keywords - Ligandi

    Actin-binding, Calmodulin-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calponin-2
    Alternative name(s):
    Calponin H2, smooth muscle
    Neutral calponin
    Gene namesi
    Name:CNN2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:2156. CNN2.

    Subcellular locationi

    GO - Cellular componenti

    1. cell-cell junction Source: ProtInc
    2. cytoskeleton Source: ProtInc
    3. extracellular vesicular exosome Source: UniProt
    4. membrane Source: UniProtKB
    5. stress fiber Source: MGI

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA26666.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed3 Publications
    Chaini2 – 309308Calponin-2PRO_0000204773Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine2 Publications
    Modified residuei8 – 81N6-acetyllysine1 Publication
    Modified residuei25 – 251N6-acetyllysine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ99439.
    PaxDbiQ99439.
    PeptideAtlasiQ99439.
    PRIDEiQ99439.

    2D gel databases

    OGPiQ99439.

    PTM databases

    PhosphoSiteiQ99439.

    Expressioni

    Tissue specificityi

    Heart and smooth muscle.

    Gene expression databases

    ArrayExpressiQ99439.
    BgeeiQ99439.
    CleanExiHS_CNN2.
    GenevestigatoriQ99439.

    Organism-specific databases

    HPAiHPA049095.

    Interactioni

    Protein-protein interaction databases

    BioGridi107665. 15 interactions.
    IntActiQ99439. 1 interaction.
    STRINGi9606.ENSP00000263097.

    Structurei

    Secondary structure

    1
    309
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi30 – 4213
    Helixi50 – 556
    Helixi59 – 679
    Helixi81 – 9818
    Beta strandi102 – 1043
    Helixi108 – 1125
    Helixi118 – 13114
    Helixi132 – 1343

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1WYNNMR-A20-152[»]
    ProteinModelPortaliQ99439.
    SMRiQ99439. Positions 13-153.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ99439.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini28 – 131104CHPROSITE-ProRule annotationAdd
    BLAST
    Repeati166 – 19126Calponin-like 1Add
    BLAST
    Repeati206 – 23126Calponin-like 2Add
    BLAST
    Repeati245 – 26925Calponin-like 3Add
    BLAST

    Sequence similaritiesi

    Belongs to the calponin family.Curated
    Contains 3 calponin-like repeats.PROSITE-ProRule annotation
    Contains 1 CH (calponin-homology) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG5199.
    HOGENOMiHOG000232113.
    HOVERGENiHBG005186.
    InParanoidiQ99439.
    OrthoDBiEOG71P2B3.
    PhylomeDBiQ99439.
    TreeFamiTF313921.

    Family and domain databases

    Gene3Di1.10.418.10. 1 hit.
    InterProiIPR001997. Calponin.
    IPR000557. Calponin_repeat.
    IPR001715. CH-domain.
    IPR003096. SM22_calponin.
    [Graphical view]
    PfamiPF00402. Calponin. 3 hits.
    PF00307. CH. 1 hit.
    [Graphical view]
    PRINTSiPR00889. CALPONIN.
    PR00888. SM22CALPONIN.
    SMARTiSM00033. CH. 1 hit.
    [Graphical view]
    SUPFAMiSSF47576. SSF47576. 1 hit.
    PROSITEiPS01052. CALPONIN_1. 3 hits.
    PS51122. CALPONIN_2. 3 hits.
    PS50021. CH. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q99439-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSSTQFNKGP SYGLSAEVKN RLLSKYDPQK EAELRTWIEG LTGLSIGPDF    50
    QKGLKDGTIL CTLMNKLQPG SVPKINRSMQ NWHQLENLSN FIKAMVSYGM 100
    NPVDLFEAND LFESGNMTQV QVSLLALAGK AKTKGLQSGV DIGVKYSEKQ 150
    ERNFDDATMK AGQCVIGLQM GTNKCASQSG MTAYGTRRHL YDPKNHILPP 200
    MDHSTISLQM GTNKCASQVG MTAPGTRRHI YDTKLGTDKC DNSSMSLQMG 250
    YTQGANQSGQ VFGLGRQIYD PKYCPQGTVA DGAPSGTGDC PDPGEVPEYP 300
    PYYQEEAGY 309
    Length:309
    Mass (Da):33,697
    Last modified:January 23, 2007 - v4
    Checksum:i0D767E9040190A5F
    GO
    Isoform 2 (identifier: Q99439-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         131-169: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:270
    Mass (Da):29,500
    Checksum:i6CCEFDE1EE200798
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti52 – 521K → R in BAA20887. (PubMed:9001224)Curated
    Sequence conflicti142 – 1421I → V in BAA20887. (PubMed:9001224)Curated
    Sequence conflicti165 – 1651V → G in BAA20887. (PubMed:9001224)Curated
    Sequence conflicti182 – 1832TA → HL in BAA20887. (PubMed:9001224)Curated
    Sequence conflicti187 – 20216RRHLY…LPPMD → AGISMTPRTISCPPWT in BAA20887. (PubMed:9001224)CuratedAdd
    BLAST

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei131 – 16939Missing in isoform 2. 1 PublicationVSP_042941Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D83735 mRNA. Translation: BAA12090.1.
    AK126391 mRNA. Translation: BAG54322.1.
    AC004528 Genomic DNA. No translation available.
    AC011558 Genomic DNA. No translation available.
    CH471139 Genomic DNA. Translation: EAW69565.1.
    CH471139 Genomic DNA. Translation: EAW69567.1.
    CH471139 Genomic DNA. Translation: EAW69570.1.
    BC141818 mRNA. Translation: AAI41819.1.
    BC141833 mRNA. Translation: AAI41834.1.
    D86059 mRNA. Translation: BAA20887.1.
    CCDSiCCDS12053.1. [Q99439-1]
    CCDS12054.1. [Q99439-2]
    PIRiJC4906.
    RefSeqiNP_004359.1. NM_004368.2. [Q99439-1]
    NP_958434.1. NM_201277.1. [Q99439-2]
    UniGeneiHs.651512.

    Genome annotation databases

    EnsembliENST00000263097; ENSP00000263097; ENSG00000064666. [Q99439-1]
    ENST00000348419; ENSP00000340129; ENSG00000064666. [Q99439-2]
    GeneIDi1265.
    KEGGihsa:1265.
    UCSCiuc002lqu.3. human. [Q99439-1]
    uc002lqv.3. human. [Q99439-2]

    Polymorphism databases

    DMDMi6226844.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D83735 mRNA. Translation: BAA12090.1 .
    AK126391 mRNA. Translation: BAG54322.1 .
    AC004528 Genomic DNA. No translation available.
    AC011558 Genomic DNA. No translation available.
    CH471139 Genomic DNA. Translation: EAW69565.1 .
    CH471139 Genomic DNA. Translation: EAW69567.1 .
    CH471139 Genomic DNA. Translation: EAW69570.1 .
    BC141818 mRNA. Translation: AAI41819.1 .
    BC141833 mRNA. Translation: AAI41834.1 .
    D86059 mRNA. Translation: BAA20887.1 .
    CCDSi CCDS12053.1. [Q99439-1 ]
    CCDS12054.1. [Q99439-2 ]
    PIRi JC4906.
    RefSeqi NP_004359.1. NM_004368.2. [Q99439-1 ]
    NP_958434.1. NM_201277.1. [Q99439-2 ]
    UniGenei Hs.651512.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1WYN NMR - A 20-152 [» ]
    ProteinModelPortali Q99439.
    SMRi Q99439. Positions 13-153.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 107665. 15 interactions.
    IntActi Q99439. 1 interaction.
    STRINGi 9606.ENSP00000263097.

    PTM databases

    PhosphoSitei Q99439.

    Polymorphism databases

    DMDMi 6226844.

    2D gel databases

    OGPi Q99439.

    Proteomic databases

    MaxQBi Q99439.
    PaxDbi Q99439.
    PeptideAtlasi Q99439.
    PRIDEi Q99439.

    Protocols and materials databases

    DNASUi 1265.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000263097 ; ENSP00000263097 ; ENSG00000064666 . [Q99439-1 ]
    ENST00000348419 ; ENSP00000340129 ; ENSG00000064666 . [Q99439-2 ]
    GeneIDi 1265.
    KEGGi hsa:1265.
    UCSCi uc002lqu.3. human. [Q99439-1 ]
    uc002lqv.3. human. [Q99439-2 ]

    Organism-specific databases

    CTDi 1265.
    GeneCardsi GC19P001026.
    HGNCi HGNC:2156. CNN2.
    HPAi HPA049095.
    MIMi 602373. gene.
    neXtProti NX_Q99439.
    PharmGKBi PA26666.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5199.
    HOGENOMi HOG000232113.
    HOVERGENi HBG005186.
    InParanoidi Q99439.
    OrthoDBi EOG71P2B3.
    PhylomeDBi Q99439.
    TreeFami TF313921.

    Miscellaneous databases

    ChiTaRSi CNN2. human.
    EvolutionaryTracei Q99439.
    GenomeRNAii 1265.
    NextBioi 5119.
    PROi Q99439.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q99439.
    Bgeei Q99439.
    CleanExi HS_CNN2.
    Genevestigatori Q99439.

    Family and domain databases

    Gene3Di 1.10.418.10. 1 hit.
    InterProi IPR001997. Calponin.
    IPR000557. Calponin_repeat.
    IPR001715. CH-domain.
    IPR003096. SM22_calponin.
    [Graphical view ]
    Pfami PF00402. Calponin. 3 hits.
    PF00307. CH. 1 hit.
    [Graphical view ]
    PRINTSi PR00889. CALPONIN.
    PR00888. SM22CALPONIN.
    SMARTi SM00033. CH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47576. SSF47576. 1 hit.
    PROSITEi PS01052. CALPONIN_1. 3 hits.
    PS51122. CALPONIN_2. 3 hits.
    PS50021. CH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of human non-smooth muscle calponin."
      Masuda H., Tanaka K., Takagi M., Ohgami K., Sakamaki T., Shibata N., Takahashi K.
      J. Biochem. 120:415-424(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Heart.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Uterus.
    3. "The DNA sequence and biology of human chromosome 19."
      Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
      , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
      Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    6. "A unique downregulation of h2-calponin gene expression in Down syndrome: a possible attenuation mechanism for fetal survival by methylation at the CpG island in the trisomic chromosome 21."
      Kuromitsu J., Yamashita H., Kataoka H., Takahara T., Muramatsu M., Sekine T., Okamoto N., Furuichi Y., Hayashizaki Y.
      Mol. Cell. Biol. 17:707-712(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-202 (ISOFORM 1).
      Tissue: Placenta.
    7. "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."
      Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J.
      Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-21.
      Tissue: Platelet.
    8. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
      Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
      Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2; LYS-8 AND LYS-25, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
    9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Solution structure of the CH domain of human calponin-2."
      RIKEN structural genomics initiative (RSGI)
      Submitted (AUG-2005) to the PDB data bank
      Cited for: STRUCTURE BY NMR OF 20-152.

    Entry informationi

    Entry nameiCNN2_HUMAN
    AccessioniPrimary (citable) accession number: Q99439
    Secondary accession number(s): A5D8U8
    , A6NFI4, D6W5X9, Q92578
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 140 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3