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Protein

Calponin-2

Gene

CNN2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.

GO - Molecular functioni

  • actin binding Source: ProtInc
  • cadherin binding Source: BHF-UCL
  • calmodulin binding Source: UniProtKB-KW

GO - Biological processi

  • actomyosin structure organization Source: InterPro
  • cellular response to mechanical stimulus Source: MGI
  • cytoskeleton organization Source: ProtInc
  • interleukin-12-mediated signaling pathway Source: Reactome
  • neutrophil degranulation Source: Reactome
  • regulation of actin filament-based process Source: MGI

Keywordsi

Molecular functionActin-binding, Calmodulin-binding

Enzyme and pathway databases

ReactomeiR-HSA-6798695 Neutrophil degranulation
R-HSA-8950505 Gene and protein expression by JAK-STAT signaling after Interleukin-12 stimulation

Names & Taxonomyi

Protein namesi
Recommended name:
Calponin-2
Alternative name(s):
Calponin H2, smooth muscle
Neutral calponin
Gene namesi
Name:CNN2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000064666.14
HGNCiHGNC:2156 CNN2
MIMi602373 gene
neXtProtiNX_Q99439

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

DisGeNETi1265
OpenTargetsiENSG00000064666
PharmGKBiPA26666

Polymorphism and mutation databases

BioMutaiCNN2
DMDMi6226844

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00002047732 – 309Calponin-2Add BLAST308

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei8N6-acetyllysineCombined sources1
Modified residuei25N6-acetyllysineCombined sources1
Modified residuei138PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ99439
MaxQBiQ99439
PaxDbiQ99439
PeptideAtlasiQ99439
PRIDEiQ99439

2D gel databases

OGPiQ99439

PTM databases

iPTMnetiQ99439
PhosphoSitePlusiQ99439
SwissPalmiQ99439

Expressioni

Tissue specificityi

Heart and smooth muscle.

Gene expression databases

BgeeiENSG00000064666
CleanExiHS_CNN2
ExpressionAtlasiQ99439 baseline and differential
GenevisibleiQ99439 HS

Organism-specific databases

HPAiHPA049095

Interactioni

GO - Molecular functioni

  • actin binding Source: ProtInc
  • cadherin binding Source: BHF-UCL
  • calmodulin binding Source: UniProtKB-KW

Protein-protein interaction databases

BioGridi107665, 29 interactors
IntActiQ99439, 6 interactors
MINTiQ99439
STRINGi9606.ENSP00000263097

Structurei

Secondary structure

1309
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi30 – 42Combined sources13
Helixi50 – 55Combined sources6
Helixi59 – 67Combined sources9
Helixi81 – 98Combined sources18
Beta strandi102 – 104Combined sources3
Helixi108 – 112Combined sources5
Helixi118 – 131Combined sources14
Helixi132 – 134Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WYNNMR-A20-152[»]
ProteinModelPortaliQ99439
SMRiQ99439
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ99439

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 132Calponin-homology (CH)PROSITE-ProRule annotationAdd BLAST105
Repeati166 – 191Calponin-like 1Add BLAST26
Repeati206 – 231Calponin-like 2Add BLAST26
Repeati245 – 269Calponin-like 3Add BLAST25

Sequence similaritiesi

Belongs to the calponin family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2046 Eukaryota
COG5199 LUCA
GeneTreeiENSGT00550000074447
HOGENOMiHOG000232113
HOVERGENiHBG005186
InParanoidiQ99439
PhylomeDBiQ99439
TreeFamiTF313921

Family and domain databases

CDDicd00014 CH, 1 hit
Gene3Di1.10.418.10, 1 hit
InterProiView protein in InterPro
IPR001997 Calponin/LIMCH1
IPR000557 Calponin_repeat
IPR001715 CH-domain
IPR036872 CH_dom_sf
IPR029974 CNN2
IPR003096 SM22_calponin
PANTHERiPTHR18959:SF47 PTHR18959:SF47, 1 hit
PfamiView protein in Pfam
PF00402 Calponin, 3 hits
PF00307 CH, 1 hit
PRINTSiPR00889 CALPONIN
PR00888 SM22CALPONIN
SMARTiView protein in SMART
SM00033 CH, 1 hit
SUPFAMiSSF47576 SSF47576, 1 hit
PROSITEiView protein in PROSITE
PS01052 CALPONIN_1, 3 hits
PS51122 CALPONIN_2, 3 hits
PS50021 CH, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q99439-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSTQFNKGP SYGLSAEVKN RLLSKYDPQK EAELRTWIEG LTGLSIGPDF
60 70 80 90 100
QKGLKDGTIL CTLMNKLQPG SVPKINRSMQ NWHQLENLSN FIKAMVSYGM
110 120 130 140 150
NPVDLFEAND LFESGNMTQV QVSLLALAGK AKTKGLQSGV DIGVKYSEKQ
160 170 180 190 200
ERNFDDATMK AGQCVIGLQM GTNKCASQSG MTAYGTRRHL YDPKNHILPP
210 220 230 240 250
MDHSTISLQM GTNKCASQVG MTAPGTRRHI YDTKLGTDKC DNSSMSLQMG
260 270 280 290 300
YTQGANQSGQ VFGLGRQIYD PKYCPQGTVA DGAPSGTGDC PDPGEVPEYP

PYYQEEAGY
Length:309
Mass (Da):33,697
Last modified:January 23, 2007 - v4
Checksum:i0D767E9040190A5F
GO
Isoform 2 (identifier: Q99439-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     131-169: Missing.

Note: No experimental confirmation available.
Show »
Length:270
Mass (Da):29,500
Checksum:i6CCEFDE1EE200798
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti52K → R in BAA20887 (PubMed:9001224).Curated1
Sequence conflicti142I → V in BAA20887 (PubMed:9001224).Curated1
Sequence conflicti165V → G in BAA20887 (PubMed:9001224).Curated1
Sequence conflicti182 – 183TA → HL in BAA20887 (PubMed:9001224).Curated2
Sequence conflicti187 – 202RRHLY…LPPMD → AGISMTPRTISCPPWT in BAA20887 (PubMed:9001224).CuratedAdd BLAST16

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_042941131 – 169Missing in isoform 2. 1 PublicationAdd BLAST39

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83735 mRNA Translation: BAA12090.1
AK126391 mRNA Translation: BAG54322.1
AC004528 Genomic DNA No translation available.
AC011558 Genomic DNA No translation available.
CH471139 Genomic DNA Translation: EAW69565.1
CH471139 Genomic DNA Translation: EAW69567.1
CH471139 Genomic DNA Translation: EAW69570.1
BC141818 mRNA Translation: AAI41819.1
BC141833 mRNA Translation: AAI41834.1
D86059 mRNA Translation: BAA20887.1
CCDSiCCDS12053.1 [Q99439-1]
CCDS12054.1 [Q99439-2]
PIRiJC4906
RefSeqiNP_001290428.1, NM_001303499.1
NP_001290430.1, NM_001303501.1
NP_004359.1, NM_004368.3 [Q99439-1]
NP_958434.1, NM_201277.2 [Q99439-2]
UniGeneiHs.651512

Genome annotation databases

EnsembliENST00000263097; ENSP00000263097; ENSG00000064666 [Q99439-1]
ENST00000348419; ENSP00000340129; ENSG00000064666 [Q99439-2]
GeneIDi1265
KEGGihsa:1265
UCSCiuc002lqu.4 human [Q99439-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCNN2_HUMAN
AccessioniPrimary (citable) accession number: Q99439
Secondary accession number(s): A5D8U8
, A6NFI4, D6W5X9, Q92578
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 171 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

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