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Q99439

- CNN2_HUMAN

UniProt

Q99439 - CNN2_HUMAN

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Protein
Calponin-2
Gene
CNN2
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.

GO - Molecular functioni

  1. actin binding Source: ProtInc

GO - Biological processi

  1. actomyosin structure organization Source: InterPro
  2. cellular response to mechanical stimulus Source: MGI
  3. cytoskeleton organization Source: ProtInc
  4. hemopoiesis Source: Ensembl
  5. negative regulation of cell migration Source: Ensembl
  6. negative regulation of phagocytosis Source: Ensembl
  7. positive regulation of gene expression Source: Ensembl
  8. regulation of actin filament-based process Source: MGI
  9. regulation of cell proliferation Source: Ensembl
  10. wound healing Source: Ensembl
Complete GO annotation...

Keywords - Ligandi

Actin-binding, Calmodulin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Calponin-2
Alternative name(s):
Calponin H2, smooth muscle
Neutral calponin
Gene namesi
Name:CNN2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 19

Organism-specific databases

HGNCiHGNC:2156. CNN2.

Subcellular locationi

GO - Cellular componenti

  1. cell-cell junction Source: ProtInc
  2. cytoskeleton Source: ProtInc
  3. extracellular vesicular exosome Source: UniProt
  4. stress fiber Source: MGI
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA26666.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 309308Calponin-2
PRO_0000204773Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine2 Publications
Modified residuei8 – 81N6-acetyllysine1 Publication
Modified residuei25 – 251N6-acetyllysine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ99439.
PaxDbiQ99439.
PeptideAtlasiQ99439.
PRIDEiQ99439.

2D gel databases

OGPiQ99439.

PTM databases

PhosphoSiteiQ99439.

Expressioni

Tissue specificityi

Heart and smooth muscle.

Gene expression databases

ArrayExpressiQ99439.
BgeeiQ99439.
CleanExiHS_CNN2.
GenevestigatoriQ99439.

Organism-specific databases

HPAiHPA049095.

Interactioni

Protein-protein interaction databases

BioGridi107665. 15 interactions.
IntActiQ99439. 1 interaction.
STRINGi9606.ENSP00000263097.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi30 – 4213
Helixi50 – 556
Helixi59 – 679
Helixi81 – 9818
Beta strandi102 – 1043
Helixi108 – 1125
Helixi118 – 13114
Helixi132 – 1343

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WYNNMR-A20-152[»]
ProteinModelPortaliQ99439.
SMRiQ99439. Positions 13-153.

Miscellaneous databases

EvolutionaryTraceiQ99439.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini28 – 131104CH
Add
BLAST
Repeati166 – 19126Calponin-like 1
Add
BLAST
Repeati206 – 23126Calponin-like 2
Add
BLAST
Repeati245 – 26925Calponin-like 3
Add
BLAST

Sequence similaritiesi

Belongs to the calponin family.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5199.
HOGENOMiHOG000232113.
HOVERGENiHBG005186.
InParanoidiQ99439.
OrthoDBiEOG71P2B3.
PhylomeDBiQ99439.
TreeFamiTF313921.

Family and domain databases

Gene3Di1.10.418.10. 1 hit.
InterProiIPR001997. Calponin.
IPR000557. Calponin_repeat.
IPR001715. CH-domain.
IPR003096. SM22_calponin.
[Graphical view]
PfamiPF00402. Calponin. 3 hits.
PF00307. CH. 1 hit.
[Graphical view]
PRINTSiPR00889. CALPONIN.
PR00888. SM22CALPONIN.
SMARTiSM00033. CH. 1 hit.
[Graphical view]
SUPFAMiSSF47576. SSF47576. 1 hit.
PROSITEiPS01052. CALPONIN_1. 3 hits.
PS51122. CALPONIN_2. 3 hits.
PS50021. CH. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q99439-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSSTQFNKGP SYGLSAEVKN RLLSKYDPQK EAELRTWIEG LTGLSIGPDF    50
QKGLKDGTIL CTLMNKLQPG SVPKINRSMQ NWHQLENLSN FIKAMVSYGM 100
NPVDLFEAND LFESGNMTQV QVSLLALAGK AKTKGLQSGV DIGVKYSEKQ 150
ERNFDDATMK AGQCVIGLQM GTNKCASQSG MTAYGTRRHL YDPKNHILPP 200
MDHSTISLQM GTNKCASQVG MTAPGTRRHI YDTKLGTDKC DNSSMSLQMG 250
YTQGANQSGQ VFGLGRQIYD PKYCPQGTVA DGAPSGTGDC PDPGEVPEYP 300
PYYQEEAGY 309
Length:309
Mass (Da):33,697
Last modified:January 23, 2007 - v4
Checksum:i0D767E9040190A5F
GO
Isoform 2 (identifier: Q99439-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     131-169: Missing.

Note: No experimental confirmation available.

Show »
Length:270
Mass (Da):29,500
Checksum:i6CCEFDE1EE200798
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei131 – 16939Missing in isoform 2.
VSP_042941Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti52 – 521K → R in BAA20887. 1 Publication
Sequence conflicti142 – 1421I → V in BAA20887. 1 Publication
Sequence conflicti165 – 1651V → G in BAA20887. 1 Publication
Sequence conflicti182 – 1832TA → HL in BAA20887. 1 Publication
Sequence conflicti187 – 20216RRHLY…LPPMD → AGISMTPRTISCPPWT in BAA20887. 1 Publication
Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D83735 mRNA. Translation: BAA12090.1.
AK126391 mRNA. Translation: BAG54322.1.
AC004528 Genomic DNA. No translation available.
AC011558 Genomic DNA. No translation available.
CH471139 Genomic DNA. Translation: EAW69565.1.
CH471139 Genomic DNA. Translation: EAW69567.1.
CH471139 Genomic DNA. Translation: EAW69570.1.
BC141818 mRNA. Translation: AAI41819.1.
BC141833 mRNA. Translation: AAI41834.1.
D86059 mRNA. Translation: BAA20887.1.
CCDSiCCDS12053.1. [Q99439-1]
CCDS12054.1. [Q99439-2]
PIRiJC4906.
RefSeqiNP_004359.1. NM_004368.2. [Q99439-1]
NP_958434.1. NM_201277.1. [Q99439-2]
UniGeneiHs.651512.

Genome annotation databases

EnsembliENST00000263097; ENSP00000263097; ENSG00000064666. [Q99439-1]
ENST00000348419; ENSP00000340129; ENSG00000064666. [Q99439-2]
GeneIDi1265.
KEGGihsa:1265.
UCSCiuc002lqu.3. human. [Q99439-1]
uc002lqv.3. human. [Q99439-2]

Polymorphism databases

DMDMi6226844.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D83735 mRNA. Translation: BAA12090.1 .
AK126391 mRNA. Translation: BAG54322.1 .
AC004528 Genomic DNA. No translation available.
AC011558 Genomic DNA. No translation available.
CH471139 Genomic DNA. Translation: EAW69565.1 .
CH471139 Genomic DNA. Translation: EAW69567.1 .
CH471139 Genomic DNA. Translation: EAW69570.1 .
BC141818 mRNA. Translation: AAI41819.1 .
BC141833 mRNA. Translation: AAI41834.1 .
D86059 mRNA. Translation: BAA20887.1 .
CCDSi CCDS12053.1. [Q99439-1 ]
CCDS12054.1. [Q99439-2 ]
PIRi JC4906.
RefSeqi NP_004359.1. NM_004368.2. [Q99439-1 ]
NP_958434.1. NM_201277.1. [Q99439-2 ]
UniGenei Hs.651512.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1WYN NMR - A 20-152 [» ]
ProteinModelPortali Q99439.
SMRi Q99439. Positions 13-153.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 107665. 15 interactions.
IntActi Q99439. 1 interaction.
STRINGi 9606.ENSP00000263097.

PTM databases

PhosphoSitei Q99439.

Polymorphism databases

DMDMi 6226844.

2D gel databases

OGPi Q99439.

Proteomic databases

MaxQBi Q99439.
PaxDbi Q99439.
PeptideAtlasi Q99439.
PRIDEi Q99439.

Protocols and materials databases

DNASUi 1265.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000263097 ; ENSP00000263097 ; ENSG00000064666 . [Q99439-1 ]
ENST00000348419 ; ENSP00000340129 ; ENSG00000064666 . [Q99439-2 ]
GeneIDi 1265.
KEGGi hsa:1265.
UCSCi uc002lqu.3. human. [Q99439-1 ]
uc002lqv.3. human. [Q99439-2 ]

Organism-specific databases

CTDi 1265.
GeneCardsi GC19P001026.
HGNCi HGNC:2156. CNN2.
HPAi HPA049095.
MIMi 602373. gene.
neXtProti NX_Q99439.
PharmGKBi PA26666.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5199.
HOGENOMi HOG000232113.
HOVERGENi HBG005186.
InParanoidi Q99439.
OrthoDBi EOG71P2B3.
PhylomeDBi Q99439.
TreeFami TF313921.

Miscellaneous databases

ChiTaRSi CNN2. human.
EvolutionaryTracei Q99439.
GenomeRNAii 1265.
NextBioi 5119.
PROi Q99439.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q99439.
Bgeei Q99439.
CleanExi HS_CNN2.
Genevestigatori Q99439.

Family and domain databases

Gene3Di 1.10.418.10. 1 hit.
InterProi IPR001997. Calponin.
IPR000557. Calponin_repeat.
IPR001715. CH-domain.
IPR003096. SM22_calponin.
[Graphical view ]
Pfami PF00402. Calponin. 3 hits.
PF00307. CH. 1 hit.
[Graphical view ]
PRINTSi PR00889. CALPONIN.
PR00888. SM22CALPONIN.
SMARTi SM00033. CH. 1 hit.
[Graphical view ]
SUPFAMi SSF47576. SSF47576. 1 hit.
PROSITEi PS01052. CALPONIN_1. 3 hits.
PS51122. CALPONIN_2. 3 hits.
PS50021. CH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of human non-smooth muscle calponin."
    Masuda H., Tanaka K., Takagi M., Ohgami K., Sakamaki T., Shibata N., Takahashi K.
    J. Biochem. 120:415-424(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Heart.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Uterus.
  3. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  6. "A unique downregulation of h2-calponin gene expression in Down syndrome: a possible attenuation mechanism for fetal survival by methylation at the CpG island in the trisomic chromosome 21."
    Kuromitsu J., Yamashita H., Kataoka H., Takahara T., Muramatsu M., Sekine T., Okamoto N., Furuichi Y., Hayashizaki Y.
    Mol. Cell. Biol. 17:707-712(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-202 (ISOFORM 1).
    Tissue: Placenta.
  7. "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."
    Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J.
    Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-21.
    Tissue: Platelet.
  8. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
    Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
    Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2; LYS-8 AND LYS-25, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Solution structure of the CH domain of human calponin-2."
    RIKEN structural genomics initiative (RSGI)
    Submitted (AUG-2005) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 20-152.

Entry informationi

Entry nameiCNN2_HUMAN
AccessioniPrimary (citable) accession number: Q99439
Secondary accession number(s): A5D8U8
, A6NFI4, D6W5X9, Q92578
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 139 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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