Q99437 (VATO_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
V-type proton ATPase 21 kDa proteolipid subunit
Short name=V-ATPase 21 kDa proteolipid subunit

Alternative name(s):
Vacuolar proton pump 21 kDa proteolipid subunit
hATPL

Gene names

Name:	ATP6V0B
Synonyms:	ATP6F

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	205 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Proton-conducting pore forming subunit of the membrane integral V0 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.
Subcellular location	Vacuole membrane; Multi-pass membrane protein.
Tissue specificity	Ubiquitous.
Sequence similarities	Belongs to the V-ATPase proteolipid subunit family.

Ontologies

Keywords
Biological process	Hydrogen ion transport Ion transport Transport
Cellular component	Membrane Vacuole
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ATP hydrolysis coupled proton transport Inferred from electronic annotation. Source: InterPro cellular iron ion homeostasis Traceable author statement. Source: Reactome insulin receptor signaling pathway Traceable author statement. Source: Reactome transferrin transport Traceable author statement. Source: Reactome
Cellular component	endosome membrane Traceable author statement. Source: Reactome integral to membrane Traceable author statement. Source: ProtInc proton-transporting V-type ATPase, V0 domain Inferred from electronic annotation. Source: InterPro vacuolar membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	hydrogen ion transmembrane transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 205	205	V-type proton ATPase 21 kDa proteolipid subunit		PRO_0000071777
Regions
	Topological domain	1 – 3	3	Lumenal Potential
	Transmembrane	4 – 24	21	Helical; Potential
	Topological domain	25 – 51	27	Cytoplasmic Potential
	Transmembrane	52 – 72	21	Helical; Potential
	Topological domain	73 – 90	18	Lumenal Potential
	Transmembrane	91 – 111	21	Helical; Potential
	Topological domain	112 – 137	26	Cytoplasmic Potential
	Transmembrane	138 – 158	21	Helical; Potential
	Topological domain	159 – 175	17	Lumenal Potential
	Transmembrane	176 – 196	21	Helical; Potential
	Topological domain	197 – 205	9	Cytoplasmic Potential
Sites
	Site	98	1	Essential for proton translocation By similarity
Natural variations
	Natural variant	155	1	V → M in a breast cancer sample; somatic mutation. Ref.4		VAR_035703

Sequences

	Sequence		Length	Mass (Da)
	Q99437 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: F17C688D068A2785	FASTA	205	21,406
	10 20 30 40 50 60 MTGLALLYSG VFVAFWACAL AVGVCYTIFD LGFRFDVAWF LTETSPFMWS NLGIGLAISL 70 80 90 100 110 120 SVVGAAWGIY ITGSSIIGGG VKAPRIKTKN LVSIIFCEAV AIYGIIMAIV ISNMAEPFSA 130 140 150 160 170 180 TDPKAIGHRN YHAGYSMFGA GLTVGLSNLF CGVCVGIVGS GAALADAQNP SLFVKILIVE 190 200 IFGSAIGLFG VIVAILQTSR VKMGD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of the gene encoding a second proteolipid subunit of human vacuolar H(+)-ATPase (ATP6F)." Nishigori H., Yamada S., Tomura H., Fernald A.A., le Beau M.M., Takeuchi T., Takeda J. Genomics 50:222-228(1998) [PubMed: 9653649] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney.
[2]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Muscle.
[4]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed: 16959974] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] MET-155.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D89052 mRNA. Translation: BAA13753.1. BT007151 mRNA. Translation: AAP35815.1. BC000423 mRNA. Translation: AAH00423.1. BC005876 mRNA. Translation: AAH05876.1.
IPI	IPI00015261.
RefSeq	NP_001034546.1. NM_001039457.1. NP_004038.1. NM_004047.3.
UniGene	Hs.596514.
3D structure databases
ProteinModelPortal	Q99437.
SMR	Q99437. Positions 49-112, 134-198.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q99437. 3 interactions.
STRING	Q99437.
Polymorphism databases
DMDM	6136172.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000236067; ENSP00000236067; ENSG00000117410.
GeneID	533.
KEGG	hsa:533.
UCSC	uc001cld.1. human.
Organism-specific databases
CTD	533.
GeneCards	GC01P044440.
H-InvDB	HIX0000513.
HGNC	HGNC:861. ATP6V0B.
MIM	603717. gene.
neXtProt	NX_Q99437.
GenAtlas	Search...
Phylogenomic databases
GeneTree	ENSGT00550000075120.
HOGENOM	HBG325773.
HOVERGEN	HBG105033.
InParanoid	Q99437.
OMA	VNLFCGI.
OrthoDB	EOG4ZW5C4.
PhylomeDB	Q99437.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction. REACT_15518. Transmembrane transport of small molecules.
Gene expression databases
ArrayExpress	Q99437.
Bgee	Q99437.
CleanEx	HS_ATP6V0B.
Genevestigator	Q99437.
GermOnline	ENSG00000117410. Homo sapiens.
Family and domain databases
InterPro	IPR002379. ATPase_F0/V0-cplx_csu. IPR000245. ATPase_V0-cplx_csu. [Graphical view]
Gene3D	G3DSA:1.20.20.10. ATPase_F0/V0_c. 2 hits.
KO	K03661.
Pfam	PF00137. ATP-synt_C. 2 hits. [Graphical view]
PRINTS	PR00122. VACATPASE.
SUPFAM	SSF81333. ATPase_F0/V0_c. 2 hits.
ProtoNet	Search...
Other
NextBio	2211.
SOURCE	Search...

Entry information

Entry name

VATO_HUMAN

Accession

Primary (citable) accession number: Q99437

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	May 1, 1997
Last modified:	January 25, 2012
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.