Q99417 (MYCBP_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
C-Myc-binding protein

Alternative name(s):
Associate of Myc 1
Short name=AMY-1

Gene names

Name:	MYCBP
Synonyms:	AMY1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	103 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May control the transcriptional activity of MYC. Stimulates the activation of E box-dependent transcription by MYC.
Subunit structure	Binds via its C-terminal region to the N-terminal region of MYC. Associates with AKAP1/S-AKAP84. Interacts with MYCBPAP. Ref.8 Ref.9
Subcellular location	Cytoplasm. Nucleus. Mitochondrion. Note: Translocates into the nucleus in the S phase of the cell cycle upon an increase of MYC expression. Found in the mitochondria when associated with AKAP1.
Tissue specificity	Highly expressed in heart, placenta, pancreas, skeletal muscle and kidney. Also present at low levels in lung.
Sequence similarities	Belongs to the AMY1 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Mitochondrion Nucleus
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	regulation of transcription, DNA-dependent Inferred from direct assay Ref.1. Source: UniProtKB spermatogenesis Inferred from expression pattern Ref.9. Source: UniProtKB transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	centrosome Inferred from direct assay. Source: HPA cytoplasm Inferred from direct assay Ref.9Ref.1. Source: UniProtKB mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from direct assay Ref.1. Source: UniProtKB
Molecular_function	transcription coactivator activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
AKAP1	Q92667-2	2	EBI-716185,EBI-2120060
ARFGEF1	Q9Y6D6	3	EBI-716185,EBI-1044254
ARFGEF2	Q9Y6D5	5	EBI-716185,EBI-2837511
MAATS1	Q7Z4T9-4	5	EBI-716185,EBI-2119735
MYCBPAP	Q8TBZ2	4	EBI-716185,EBI-1391585

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.7

Chain

2 – 103

102

C-Myc-binding protein

PRO_0000220980

Experimental info

Sequence conflict

65

1

L → V in BAA09338. Ref.1

Sequence conflict

65

1

L → V in BAA22408. Ref.1

Secondary structure

1

.

.

.

.

103

Helix Strand Turn

Details...

Helix

60 – 90

31

Turn

91 – 93

3

Sequences

	Sequence		Length	Mass (Da)
	Q99417 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 72B9BD179A6808D0	FASTA	103	11,967
	10 20 30 40 50 60 MAHYKAADSK REQFRRYLEK SGVLDTLTKV LVALYEEPEK PNSALDFLKH HLGAATPENP 70 80 90 100 EIELLRLELA EMKEKYEAIV EENKKLKAKL AQYEPPQEEK RAE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"AMY-1, a novel C-MYC binding protein that stimulates transcription activity of C-MYC." Taira T., Maeda J., Ohishi T., Kitaura H., Yoshida S., Kato H., Ikeda M., Tamai K., Iguchi-Ariga S.M.M., Ariga H. Genes Cells 3:549-565(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Placenta.
[2]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[4]	"Human protein factory for converting the transcriptome into an in vitro-expressed proteome." Goshima N., Kawamura Y., Fukumoto A., Miura A., Honma R., Satoh R., Wakamatsu A., Yamamoto J., Kimura K., Nishikawa T., Andoh T., Iida Y., Ishikawa K., Ito E., Kagawa N., Kaminaga C., Kanehori K., Kawakami B. , Kenmochi K., Kimura R., Kobayashi M., Kuroita T., Kuwayama H., Maruyama Y., Matsuo K., Minami K., Mitsubori M., Mori M., Morishita R., Murase A., Nishikawa A., Nishikawa S., Okamoto T., Sakagami N., Sakamoto Y., Sasaki Y., Seki T., Sono S., Sugiyama A., Sumiya T., Takayama T., Takayama Y., Takeda H., Togashi T., Yahata K., Yamada H., Yanagisawa Y., Endo Y., Imamoto F., Kisu Y., Tanaka S., Isogai T., Imai J., Watanabe S., Nomura N. Nat. Methods 5:1011-1017(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Eye.
[7]	"Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J. Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-11. Tissue: Platelet.
[8]	"AMY-1, a c-Myc-binding protein, is localized in the mitochondria of sperm by association with S-AKAP84, an anchor protein of cAMP-dependent protein kinase." Furusawa M., Ohnishi T., Taira T., Iguchi-Ariga S.M.M., Ariga H. J. Biol. Chem. 276:36647-36651(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH AKAP1.
[9]	"AMAP-1, a novel testis-specific AMY-1-binding protein, is differentially expressed during the course of spermatogenesis." Yukitake H., Furusawa M., Taira T., Iguchi-Ariga S.M.M., Ariga H. Biochim. Biophys. Acta 1577:126-132(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH MYCBPAP.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]	"Crystal structure of c-myc-1 binding protein domain from Homo sapiens." RIKEN structural genomics initiative (RSGI) Submitted (APR-2008) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 42-94.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D50692 mRNA. Translation: BAA09338.1.
BT007109 mRNA. Translation: AAP35773.1.
AB007191 mRNA. Translation: BAA22408.1.
AB451287 mRNA. Translation: BAG70101.1.
AB451419 mRNA. Translation: BAG70233.1.
AK311886 mRNA. Translation: BAG34827.1.
AL139260 Genomic DNA. Translation: CAI23050.1.
BC008686 mRNA. Translation: AAH08686.1.

IPI

IPI00871174.

RefSeq

NP_036465.2. NM_012333.4.

UniGene

Hs.591506.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2YY0	X-ray	2.40	A/B/C/D	42-94	[»]

ProteinModelPortal

Q99417.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q99417. 20 interactions.

MINT

MINT-1367805.

STRING

9606.ENSP00000380702.

PTM databases

PhosphoSite

Q99417.

Polymorphism databases

DMDM

33112671.

Proteomic databases

PaxDb

Q99417.

PeptideAtlas

Q99417.

PRIDE

Q99417.

Protocols and materials databases

DNASU

26292.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000397572; ENSP00000380702; ENSG00000214114.

GeneID

26292.

KEGG

hsa:26292.

UCSC

uc001ccs.3. human.

Organism-specific databases

CTD

26292.

GeneCards

GC01M039328.

HGNC

HGNC:7554. MYCBP.

HPA

HPA041188.

MIM

606535. gene.

neXtProt

NX_Q99417.

PharmGKB

PA31354.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG68420.

HOGENOM

HOG000260967.

HOVERGEN

HBG052546.

InParanoid

Q99417.

OMA

AQYEPLQ.

OrthoDB

EOG4N8R69.

Gene expression databases

Bgee

Q99417.

CleanEx

HS_MYCBP.

Genevestigator

Q99417.

GermOnline

ENSG00000131233. Homo sapiens.

Family and domain databases

InterPro

IPR026060. AMY1.
[Graphical view]

PANTHER

PTHR13168. PTHR13168. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q99417.

GenomeRNAi

26292.

NextBio

48639.

SOURCE

Search...

Entry information

Entry name

MYCBP_HUMAN

Accession

Primary (citable) accession number: Q99417
Secondary accession number(s): B2R4N0, Q5TA64, Q96HE2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 11, 2002
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 113 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.