Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

M-protease

Gene

aprE

Organism
Bacillus clausii (strain KSM-K16)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cofactori

Ca2+Note: Binds 2 calcium ions per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi113Calcium 11
Active sitei143Charge relay system1
Metal bindingi151Calcium 11
Active sitei173Charge relay system1
Metal bindingi184Calcium 1; via carbonyl oxygen1
Metal bindingi186Calcium 11
Metal bindingi188Calcium 1; via carbonyl oxygen1
Metal bindingi190Calcium 1; via carbonyl oxygen1
Metal bindingi274Calcium 2; via carbonyl oxygen1
Metal bindingi276Calcium 2; via carbonyl oxygen1
Metal bindingi279Calcium 2; via carbonyl oxygen1
Active sitei326Charge relay system1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Ligandi

Calcium, Metal-binding

Protein family/group databases

MEROPSiS08.010.

Names & Taxonomyi

Protein namesi
Recommended name:
M-protease (EC:3.4.21.-)
Gene namesi
Name:aprE
Ordered Locus Names:ABC0761
OrganismiBacillus clausii (strain KSM-K16)
Taxonomic identifieri66692 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001168 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
PropeptideiPRO_000002714228 – 1111 PublicationAdd BLAST84
ChainiPRO_0000027143112 – 380M-proteaseAdd BLAST269

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi66692.ABC0761.

Structurei

Secondary structure

1380
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi117 – 121Combined sources5
Helixi124 – 129Combined sources6
Beta strandi138 – 144Combined sources7
Beta strandi154 – 159Combined sources6
Beta strandi170 – 172Combined sources3
Helixi173 – 182Combined sources10
Beta strandi186 – 189Combined sources4
Beta strandi198 – 203Combined sources6
Helixi213 – 225Combined sources13
Beta strandi229 – 233Combined sources5
Beta strandi237 – 239Combined sources3
Helixi242 – 254Combined sources13
Beta strandi257 – 261Combined sources5
Turni273 – 275Combined sources3
Beta strandi279 – 285Combined sources7
Beta strandi289 – 291Combined sources3
Beta strandi301 – 306Combined sources6
Beta strandi308 – 314Combined sources7
Turni315 – 317Combined sources3
Beta strandi318 – 322Combined sources5
Helixi325 – 342Combined sources18
Helixi348 – 358Combined sources11
Helixi365 – 368Combined sources4
Helixi375 – 378Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MPTX-ray2.40A112-380[»]
1WSDX-ray1.50A112-380[»]
ProteinModelPortaliQ99405.
SMRiQ99405.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ99405.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini138 – 379Peptidase S8Add BLAST242

Sequence similaritiesi

Belongs to the peptidase S8 family.Curated
Contains 1 peptidase S8 domain.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105RX7. Bacteria.
COG1404. LUCA.
HOGENOMiHOG000199176.
KOiK01342.
OMAiPNDPYFK.
OrthoDBiPOG091H03VP.

Family and domain databases

Gene3Di3.30.70.80. 1 hit.
3.40.50.200. 1 hit.
InterProiIPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR009020. Propept_inh.
IPR010259. S8pro/Inhibitor_I9.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 1 hit.
PfamiPF00082. Peptidase_S8. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
PROSITEiPS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99405-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKPLGKIVA STALLISVAF SSSIASAAEE AKEKYLIGFN EQEAVSEFVE
60 70 80 90 100
QIEANDDVAI LSEEEEVEIE LLHEFETIPV LSVELSPEDV DALELDPTIS
110 120 130 140 150
YIEEDAEVTT MAQSVPWGIS RVQAPAAHNR GLTGSGVKVA VLDTGISTHP
160 170 180 190 200
DLNIRGGASF VPGEPSTQDG NGHGTHVAGT IAALNNSIGV LGVAPSAELY
210 220 230 240 250
AVKVLGASGS GSVSSIAQGL EWAGNNGMHV ANLSLGSPSP SATLEQAVNS
260 270 280 290 300
ATSRGVLVVA ASGNSGAGSI SYPARYANAM AVGATDQNNN RASFSQYGAG
310 320 330 340 350
LDIVAPGVNV QSTYPGSTYA SLNGTSMATP HVAGVAALVK QKNPSWSNVQ
360 370 380
IRNHLKNTAT GLGNTNLYGS GLVNAEAATR
Length:380
Mass (Da):38,881
Last modified:January 4, 2005 - v2
Checksum:i9A916CFACD8E43E9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006627 Genomic DNA. Translation: BAD63300.1.
RefSeqiWP_011245616.1. NC_006582.1.

Genome annotation databases

EnsemblBacteriaiBAD63300; BAD63300; ABC0761.
KEGGibcl:ABC0761.
PATRICi18920804. VBIBacCla58185_0807.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006627 Genomic DNA. Translation: BAD63300.1.
RefSeqiWP_011245616.1. NC_006582.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MPTX-ray2.40A112-380[»]
1WSDX-ray1.50A112-380[»]
ProteinModelPortaliQ99405.
SMRiQ99405.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi66692.ABC0761.

Protein family/group databases

MEROPSiS08.010.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD63300; BAD63300; ABC0761.
KEGGibcl:ABC0761.
PATRICi18920804. VBIBacCla58185_0807.

Phylogenomic databases

eggNOGiENOG4105RX7. Bacteria.
COG1404. LUCA.
HOGENOMiHOG000199176.
KOiK01342.
OMAiPNDPYFK.
OrthoDBiPOG091H03VP.

Miscellaneous databases

EvolutionaryTraceiQ99405.

Family and domain databases

Gene3Di3.30.70.80. 1 hit.
3.40.50.200. 1 hit.
InterProiIPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR009020. Propept_inh.
IPR010259. S8pro/Inhibitor_I9.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 1 hit.
PfamiPF00082. Peptidase_S8. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
PROSITEiPS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRTM_BACSK
AccessioniPrimary (citable) accession number: Q99405
Secondary accession number(s): Q5WK05
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 4, 2005
Last modified: November 2, 2016
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.