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Protein

M-protease

Gene

aprE

Organism
Bacillus clausii (strain KSM-K16)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cofactori

Ca2+Note: Binds 2 calcium ions per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi113 – 1131Calcium 1
Active sitei143 – 1431Charge relay system
Metal bindingi151 – 1511Calcium 1
Active sitei173 – 1731Charge relay system
Metal bindingi184 – 1841Calcium 1; via carbonyl oxygen
Metal bindingi186 – 1861Calcium 1
Metal bindingi188 – 1881Calcium 1; via carbonyl oxygen
Metal bindingi190 – 1901Calcium 1; via carbonyl oxygen
Metal bindingi274 – 2741Calcium 2; via carbonyl oxygen
Metal bindingi276 – 2761Calcium 2; via carbonyl oxygen
Metal bindingi279 – 2791Calcium 2; via carbonyl oxygen
Active sitei326 – 3261Charge relay system

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BioCyciBCLA66692:GHMP-791-MONOMER.

Protein family/group databases

MEROPSiS08.010.

Names & Taxonomyi

Protein namesi
Recommended name:
M-protease (EC:3.4.21.-)
Gene namesi
Name:aprE
Ordered Locus Names:ABC0761
OrganismiBacillus clausii (strain KSM-K16)
Taxonomic identifieri66692 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001168 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence analysisAdd
BLAST
Propeptidei28 – 111841 PublicationPRO_0000027142Add
BLAST
Chaini112 – 380269M-proteasePRO_0000027143Add
BLAST

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi66692.ABC0761.

Structurei

Secondary structure

1
380
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi117 – 1215Combined sources
Helixi124 – 1296Combined sources
Beta strandi138 – 1447Combined sources
Beta strandi154 – 1596Combined sources
Beta strandi170 – 1723Combined sources
Helixi173 – 18210Combined sources
Beta strandi186 – 1894Combined sources
Beta strandi198 – 2036Combined sources
Helixi213 – 22513Combined sources
Beta strandi229 – 2335Combined sources
Beta strandi237 – 2393Combined sources
Helixi242 – 25413Combined sources
Beta strandi257 – 2615Combined sources
Turni273 – 2753Combined sources
Beta strandi279 – 2857Combined sources
Beta strandi289 – 2913Combined sources
Beta strandi301 – 3066Combined sources
Beta strandi308 – 3147Combined sources
Turni315 – 3173Combined sources
Beta strandi318 – 3225Combined sources
Helixi325 – 34218Combined sources
Helixi348 – 35811Combined sources
Helixi365 – 3684Combined sources
Helixi375 – 3784Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MPTX-ray2.40A112-380[»]
1WSDX-ray1.50A112-380[»]
ProteinModelPortaliQ99405.
SMRiQ99405. Positions 112-380.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ99405.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini138 – 379242Peptidase S8Add
BLAST

Sequence similaritiesi

Belongs to the peptidase S8 family.Curated
Contains 1 peptidase S8 domain.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105RX7. Bacteria.
COG1404. LUCA.
HOGENOMiHOG000199176.
KOiK01342.
OMAiPNDPYFK.
OrthoDBiEOG6KHG28.

Family and domain databases

Gene3Di3.30.70.80. 1 hit.
3.40.50.200. 1 hit.
InterProiIPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR009020. Propept_inh.
IPR010259. S8pro/Inhibitor_I9.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 1 hit.
PfamiPF00082. Peptidase_S8. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
PROSITEiPS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99405-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKPLGKIVA STALLISVAF SSSIASAAEE AKEKYLIGFN EQEAVSEFVE
60 70 80 90 100
QIEANDDVAI LSEEEEVEIE LLHEFETIPV LSVELSPEDV DALELDPTIS
110 120 130 140 150
YIEEDAEVTT MAQSVPWGIS RVQAPAAHNR GLTGSGVKVA VLDTGISTHP
160 170 180 190 200
DLNIRGGASF VPGEPSTQDG NGHGTHVAGT IAALNNSIGV LGVAPSAELY
210 220 230 240 250
AVKVLGASGS GSVSSIAQGL EWAGNNGMHV ANLSLGSPSP SATLEQAVNS
260 270 280 290 300
ATSRGVLVVA ASGNSGAGSI SYPARYANAM AVGATDQNNN RASFSQYGAG
310 320 330 340 350
LDIVAPGVNV QSTYPGSTYA SLNGTSMATP HVAGVAALVK QKNPSWSNVQ
360 370 380
IRNHLKNTAT GLGNTNLYGS GLVNAEAATR
Length:380
Mass (Da):38,881
Last modified:January 4, 2005 - v2
Checksum:i9A916CFACD8E43E9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006627 Genomic DNA. Translation: BAD63300.1.
RefSeqiWP_011245616.1. NC_006582.1.

Genome annotation databases

EnsemblBacteriaiBAD63300; BAD63300; ABC0761.
KEGGibcl:ABC0761.
PATRICi18920804. VBIBacCla58185_0807.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006627 Genomic DNA. Translation: BAD63300.1.
RefSeqiWP_011245616.1. NC_006582.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MPTX-ray2.40A112-380[»]
1WSDX-ray1.50A112-380[»]
ProteinModelPortaliQ99405.
SMRiQ99405. Positions 112-380.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi66692.ABC0761.

Protein family/group databases

MEROPSiS08.010.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD63300; BAD63300; ABC0761.
KEGGibcl:ABC0761.
PATRICi18920804. VBIBacCla58185_0807.

Phylogenomic databases

eggNOGiENOG4105RX7. Bacteria.
COG1404. LUCA.
HOGENOMiHOG000199176.
KOiK01342.
OMAiPNDPYFK.
OrthoDBiEOG6KHG28.

Enzyme and pathway databases

BioCyciBCLA66692:GHMP-791-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ99405.

Family and domain databases

Gene3Di3.30.70.80. 1 hit.
3.40.50.200. 1 hit.
InterProiIPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR009020. Propept_inh.
IPR010259. S8pro/Inhibitor_I9.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 1 hit.
PfamiPF00082. Peptidase_S8. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
PROSITEiPS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16."
    Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K.
    Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KSM-K16.
  2. "Purification and properties of an alkaline protease from alkalophilic Bacillus sp. KSM-K16."
    Kobayashi T., Hakamada Y., Adachi S., Hitomi J., Yoshimatsu T., Koike K., Kawai S., Ito S.
    Appl. Microbiol. Biotechnol. 43:473-481(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 112-134, CHARACTERIZATION.
  3. "Structure of a new alkaline serine protease (M-protease) from Bacillus sp. KSM-K16."
    Yamane T., Kani T., Hatanaka T., Suzuki A., Ashida T., Kobayashi T., Ito S., Yamashita O.
    Acta Crystallogr. D 51:199-206(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 112-380.

Entry informationi

Entry nameiPRTM_BACSK
AccessioniPrimary (citable) accession number: Q99405
Secondary accession number(s): Q5WK05
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 4, 2005
Last modified: July 6, 2016
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.