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Protein

M-protease

Gene

aprE

Organism
Bacillus clausii (strain KSM-K16)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cofactori

Ca2+Note: Binds 2 calcium ions per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi113Calcium 11
Active sitei143Charge relay system1
Metal bindingi151Calcium 11
Active sitei173Charge relay system1
Metal bindingi184Calcium 1; via carbonyl oxygen1
Metal bindingi186Calcium 11
Metal bindingi188Calcium 1; via carbonyl oxygen1
Metal bindingi190Calcium 1; via carbonyl oxygen1
Metal bindingi274Calcium 2; via carbonyl oxygen1
Metal bindingi276Calcium 2; via carbonyl oxygen1
Metal bindingi279Calcium 2; via carbonyl oxygen1
Active sitei326Charge relay system1

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
LigandCalcium, Metal-binding

Enzyme and pathway databases

BioCyciBCLA66692:G1G25-808-MONOMER

Protein family/group databases

MEROPSiS08.010

Names & Taxonomyi

Protein namesi
Recommended name:
M-protease (EC:3.4.21.-)
Gene namesi
Name:aprE
Ordered Locus Names:ABC0761
OrganismiBacillus clausii (strain KSM-K16)
Taxonomic identifieri66692 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001168 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
PropeptideiPRO_000002714228 – 1111 PublicationAdd BLAST84
ChainiPRO_0000027143112 – 380M-proteaseAdd BLAST269

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi66692.ABC0761

Structurei

Secondary structure

1380
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi117 – 121Combined sources5
Helixi124 – 129Combined sources6
Beta strandi138 – 144Combined sources7
Beta strandi154 – 159Combined sources6
Beta strandi170 – 172Combined sources3
Helixi173 – 182Combined sources10
Beta strandi186 – 189Combined sources4
Beta strandi198 – 203Combined sources6
Helixi213 – 225Combined sources13
Beta strandi229 – 233Combined sources5
Beta strandi237 – 239Combined sources3
Helixi242 – 254Combined sources13
Beta strandi257 – 261Combined sources5
Turni273 – 275Combined sources3
Beta strandi279 – 285Combined sources7
Beta strandi289 – 291Combined sources3
Beta strandi301 – 306Combined sources6
Beta strandi308 – 314Combined sources7
Turni315 – 317Combined sources3
Beta strandi318 – 322Combined sources5
Helixi325 – 342Combined sources18
Helixi348 – 358Combined sources11
Helixi365 – 368Combined sources4
Helixi375 – 378Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MPTX-ray2.40A112-380[»]
1WSDX-ray1.50A112-380[»]
ProteinModelPortaliQ99405
SMRiQ99405
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ99405

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini138 – 379Peptidase S8Add BLAST242

Sequence similaritiesi

Belongs to the peptidase S8 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105RX7 Bacteria
COG1404 LUCA
HOGENOMiHOG000199176
KOiK01342
OMAiNTDACSS
OrthoDBiPOG091H03VP

Family and domain databases

CDDicd07477 Peptidases_S8_Subtilisin_subse, 1 hit
Gene3Di3.30.70.80, 1 hit
3.40.50.200, 1 hit
InterProiView protein in InterPro
IPR000209 Peptidase_S8/S53_dom
IPR036852 Peptidase_S8/S53_dom_sf
IPR023827 Peptidase_S8_Asp-AS
IPR022398 Peptidase_S8_His-AS
IPR023828 Peptidase_S8_Ser-AS
IPR015500 Peptidase_S8_subtilisin-rel
IPR010259 S8pro/Inhibitor_I9
IPR037045 S8pro/Inhibitor_I9_sf
IPR034202 Subtilisin_Carlsberg-like
PfamiView protein in Pfam
PF05922 Inhibitor_I9, 1 hit
PF00082 Peptidase_S8, 1 hit
PRINTSiPR00723 SUBTILISIN
SUPFAMiSSF52743 SSF52743, 1 hit
PROSITEiView protein in PROSITE
PS00136 SUBTILASE_ASP, 1 hit
PS00137 SUBTILASE_HIS, 1 hit
PS00138 SUBTILASE_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99405-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKPLGKIVA STALLISVAF SSSIASAAEE AKEKYLIGFN EQEAVSEFVE
60 70 80 90 100
QIEANDDVAI LSEEEEVEIE LLHEFETIPV LSVELSPEDV DALELDPTIS
110 120 130 140 150
YIEEDAEVTT MAQSVPWGIS RVQAPAAHNR GLTGSGVKVA VLDTGISTHP
160 170 180 190 200
DLNIRGGASF VPGEPSTQDG NGHGTHVAGT IAALNNSIGV LGVAPSAELY
210 220 230 240 250
AVKVLGASGS GSVSSIAQGL EWAGNNGMHV ANLSLGSPSP SATLEQAVNS
260 270 280 290 300
ATSRGVLVVA ASGNSGAGSI SYPARYANAM AVGATDQNNN RASFSQYGAG
310 320 330 340 350
LDIVAPGVNV QSTYPGSTYA SLNGTSMATP HVAGVAALVK QKNPSWSNVQ
360 370 380
IRNHLKNTAT GLGNTNLYGS GLVNAEAATR
Length:380
Mass (Da):38,881
Last modified:January 4, 2005 - v2
Checksum:i9A916CFACD8E43E9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006627 Genomic DNA Translation: BAD63300.1
RefSeqiWP_011245616.1, NC_006582.1

Genome annotation databases

EnsemblBacteriaiBAD63300; BAD63300; ABC0761
GeneIDi34044654
KEGGibcl:ABC0761

Similar proteinsi

Entry informationi

Entry nameiPRTM_BACSK
AccessioniPrimary (citable) accession number: Q99405
Secondary accession number(s): Q5WK05
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 4, 2005
Last modified: March 28, 2018
This is version 130 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health